Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
29	0.000834	0.09021	b2798	ygdG	-11.41	0.005967	211 -- 245	24 -- 57	Ssb-binding protein misidentified as ExoIX	_____________210_________________________________246\n_____________\|___________________________________\|\n5'-CCA...UGUUC_________UUGUC__U_____GAAUCUU______CGCAU...UCG-3'\n______________AUUUGCUUA_____GA_GCACU_______AUUCGU\n______________\|:\|\|\|\|\|\|\|_____\|\|_\|\|\|\|\|_______\|\|\|\|\|\|\n______________UGAACGAAU_____CU_CGUGA_______UAAGCA\n3'-UUU...UACUU_________UCGUU__U_____AUUUUU_______AUGUG...CAA-5'\n_____________\|___________________________________\|\n_____________58__________________________________23\n	213 -- 219	49 -- 55	-23.2646 kcal/mol	5.57825 kcal/mol	6.27607 kcal/mol	210 246@LB@ \| \|@LB@5'-CCA...UGUUC UUGUC U GAAUCUU CGCAU...UCG-3'@LB@ AUUUGCUUA GA GCACU AUUCGU@LB@ \|:\|\|\|\|\|\|\| \|\| \|\|\|\|\| \|\|\|\|\|\|@LB@ UGAACGAAU CU CGUGA UAAGCA@LB@3'-UUU...UACUU UCGUU U AUUUUU AUGUG...CAA-5'@LB@ \| \|@LB@ 58 23@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -23.2646 kcal/mol@LB@Unfolding Energy - mRNA 5.57825 kcal/mol@LB@Unfolding Energy - sRNA 6.27607 kcal/mol@LB@@LB@Position - mRNA 211 -- 245@LB@Position - sRNA 24 -- 57@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 49 -- 55@LB@
130	0.01234	0.3033	b2958	yggN	-5.61	0.236844	227 -- 233	35 -- 41	DUF2884 family putative periplasmic protein	_____________226_____234\n_____________\|_______\|\n5'-ACG...GCGGC_______UCAGU...UUG-3'\n______________AGCACUU\n______________\|\|\|\|\|\|\|\n______________UCGUGAA\n3'-UUU...GUUCU_______UUUUU...CAA-5'\n_____________\|_______\|\n_____________42______34\n	227 -- 233	35 -- 41	-8.1936 kcal/mol	0.976472 kcal/mol	1.6033 kcal/mol	226 234@LB@ \| \|@LB@5'-ACG...GCGGC UCAGU...UUG-3'@LB@ AGCACUU@LB@ \|\|\|\|\|\|\|@LB@ UCGUGAA@LB@3'-UUU...GUUCU UUUUU...CAA-5'@LB@ \| \|@LB@ 42 34@LB@@LB@@LB@Energy -5.61 kcal/mol@LB@Hybridization Energy -8.1936 kcal/mol@LB@Unfolding Energy - mRNA 0.976472 kcal/mol@LB@Unfolding Energy - sRNA 1.6033 kcal/mol@LB@@LB@Position - mRNA 227 -- 233@LB@Position - sRNA 35 -- 41@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 35 -- 41@LB@
46	0.002046	0.1433	b3106	yhaK	-10.10	0.015122	97 -- 120	7 -- 28	redox-sensitive bicupin	_____________96_______________________121\n_____________\|________________________\|\n5'-UCC...GCCAU______CU__U_____________ACAUA...AAU-3'\n______________UUCGUU__CA_UCAGGAAAUUUGA\n______________\|\|\|\|\|\|__\|\|_:\|\|\|\|\|\|\|\|:\|\|\|\n______________AAGCAA__GU_GGUCCUUUAGACU\n3'-UUU...UUUUU______U_________________ACACAA-5'\n_____________\|________________________\|\n_____________29_______________________6\n	111 -- 117	10 -- 16	-23.1033 kcal/mol	4.40095 kcal/mol	8.59864 kcal/mol	96 121@LB@ \| \|@LB@5'-UCC...GCCAU CU U ACAUA...AAU-3'@LB@ UUCGUU CA UCAGGAAAUUUGA@LB@ \|\|\|\|\|\| \|\| :\|\|\|\|\|\|\|\|:\|\|\|@LB@ AAGCAA GU GGUCCUUUAGACU@LB@3'-UUU...UUUUU U ACACAA-5'@LB@ \| \|@LB@ 29 6@LB@@LB@@LB@Energy -10.10 kcal/mol@LB@Hybridization Energy -23.1033 kcal/mol@LB@Unfolding Energy - mRNA 4.40095 kcal/mol@LB@Unfolding Energy - sRNA 8.59864 kcal/mol@LB@@LB@Position - mRNA 97 -- 120@LB@Position - sRNA 7 -- 28@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 10 -- 16@LB@
25	0.0007384	0.09021	b3434	yhgN	-11.94	0.004015	159 -- 197	7 -- 41	UPF0056 family inner membrane protein	_____________158_____________________________________198\n_____________\|_______________________________________\|\n5'-UUU...UUGUU_____GC_______CCG__GCAAU_______________GUUAU...CGA-3'\n______________AGCAC__AGAAAGU___CG_____UAUCAGGGAAUUUGA\n______________\|\|\|\|\|__\|:\|\|\|:\|___\|\|_____:\|:\|\|\|\|:\|\|\|:\|\|\|\n______________UCGUG__UUUUUUA___GC_____GUGGUCCUUUAGACU\n3'-UUU...GUUCU_____AA_______A____AAU_________________ACACAA-5'\n_____________\|_______________________________________\|\n_____________42______________________________________6\n	188 -- 194	10 -- 16	-30.001 kcal/mol	8.83552 kcal/mol	9.22092 kcal/mol	158 198@LB@ \| \|@LB@5'-UUU...UUGUU GC CCG GCAAU GUUAU...CGA-3'@LB@ AGCAC AGAAAGU CG UAUCAGGGAAUUUGA@LB@ \|\|\|\|\| \|:\|\|\|:\| \|\| :\|:\|\|\|\|:\|\|\|:\|\|\|@LB@ UCGUG UUUUUUA GC GUGGUCCUUUAGACU@LB@3'-UUU...GUUCU AA A AAU ACACAA-5'@LB@ \| \|@LB@ 42 6@LB@@LB@@LB@Energy -11.94 kcal/mol@LB@Hybridization Energy -30.001 kcal/mol@LB@Unfolding Energy - mRNA 8.83552 kcal/mol@LB@Unfolding Energy - sRNA 9.22092 kcal/mol@LB@@LB@Position - mRNA 159 -- 197@LB@Position - sRNA 7 -- 41@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 10 -- 16@LB@
52	0.002466	0.1527	b3527	yhjJ	-8.40	0.046956	207 -- 220	25 -- 41	putative periplasmic M16 family chaperone	_____________206_______________221\n_____________\|_________________\|\n5'-CUC...UGCAG_________________ACUUU...GGC-3'\n______________GGCAC___AAAAAUUCG\n______________:\|\|\|\|___\|\|\|\|\|\|\|\|\|\n______________UCGUG___UUUUUAAGC\n3'-UUU...GUUCU_____AAU_________AAUGU...CAA-5'\n_____________\|_________________\|\n_____________42________________24\n	214 -- 220	25 -- 31	-13.4116 kcal/mol	3.36563 kcal/mol	1.64839 kcal/mol	206 221@LB@ \| \|@LB@5'-CUC...UGCAG ACUUU...GGC-3'@LB@ GGCAC AAAAAUUCG@LB@ :\|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ UCGUG UUUUUAAGC@LB@3'-UUU...GUUCU AAU AAUGU...CAA-5'@LB@ \| \|@LB@ 42 24@LB@@LB@@LB@Energy -8.40 kcal/mol@LB@Hybridization Energy -13.4116 kcal/mol@LB@Unfolding Energy - mRNA 3.36563 kcal/mol@LB@Unfolding Energy - sRNA 1.64839 kcal/mol@LB@@LB@Position - mRNA 207 -- 220@LB@Position - sRNA 25 -- 41@LB@Position Seed - mRNA 214 -- 220@LB@Position Seed - sRNA 25 -- 31@LB@
144	0.01453	0.3231	b3614	yibQ	-4.49	0.404320	261 -- 267	34 -- 40	putative polysaccharide deacetylase	_____________260_____268\n_____________\|_______\|\n5'-UGG...CCCCC_______CUGGC...UUG-3'\n______________GUACUUG\n______________\|:\|\|\|\|:\n______________CGUGAAU\n3'-UUU...UUCUU_______UUUUU...CAA-5'\n_____________\|_______\|\n_____________41______33\n	261 -- 267	34 -- 40	-7.10274 kcal/mol	0.998159 kcal/mol	1.61352 kcal/mol	260 268@LB@ \| \|@LB@5'-UGG...CCCCC CUGGC...UUG-3'@LB@ GUACUUG@LB@ \|:\|\|\|\|:@LB@ CGUGAAU@LB@3'-UUU...UUCUU UUUUU...CAA-5'@LB@ \| \|@LB@ 41 33@LB@@LB@@LB@Energy -4.49 kcal/mol@LB@Hybridization Energy -7.10274 kcal/mol@LB@Unfolding Energy - mRNA 0.998159 kcal/mol@LB@Unfolding Energy - sRNA 1.61352 kcal/mol@LB@@LB@Position - mRNA 261 -- 267@LB@Position - sRNA 34 -- 40@LB@Position Seed - mRNA 261 -- 267@LB@Position Seed - sRNA 34 -- 40@LB@
12	0.0001753	0.04706	b3676	yidH	-12.46	0.002725	279 -- 300	7 -- 26	DUF202 family inner membrane protein	_____________278___________________300\n_____________\|_____________________\|\n5'-GCC...GGAUC___AC__________UU_____-3'\n______________CGU__UGCACUAGGA__UCUGG\n______________\|\|\|__\|:\|\|\|:\|\|\|\|__\|\|\|\|:\n______________GCA__AUGUGGUCCU__AGACU\n3'-UUU...UUUAA_______________UU_____ACACAA-5'\n_____________\|______________________\|\n_____________27_____________________6\n	286 -- 292	15 -- 21	-23.8048 kcal/mol	2.98352 kcal/mol	8.36616 kcal/mol	278 300@LB@ \| \|@LB@5'-GCC...GGAUC AC UU -3'@LB@ CGU UGCACUAGGA UCUGG@LB@ \|\|\| \|:\|\|\|:\|\|\|\| \|\|\|\|:@LB@ GCA AUGUGGUCCU AGACU@LB@3'-UUU...UUUAA UU ACACAA-5'@LB@ \| \|@LB@ 27 6@LB@@LB@@LB@Energy -12.46 kcal/mol@LB@Hybridization Energy -23.8048 kcal/mol@LB@Unfolding Energy - mRNA 2.98352 kcal/mol@LB@Unfolding Energy - sRNA 8.36616 kcal/mol@LB@@LB@Position - mRNA 279 -- 300@LB@Position - sRNA 7 -- 26@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 15 -- 21@LB@
139	0.01338	0.3093	b3765	yifB	-8.91	0.033732	103 -- 139	26 -- 57	magnesium chelatase family protein and putative transcriptional regulator	_____________102______________________________________140\n_____________\|________________________________________\|\n5'-AAA...ACUUC______ACAAA___A_____AUC__C______________ACGAA...CGA-3'\n______________ACUUGC_____UAA_CAGGA___AG_CACU___AAAAUUC\n______________\|\|\|\|\|\|_____\|\|\|_\|\|:\|\|___\|\|_\|\|\|\|___\|\|\|\|\|\|\|\n______________UGAACG_____AUU_GUUCU___UC_GUGA___UUUUAAG\n3'-UUU...UACUU______A_______C_______________AUU_______CAAUG...CAA-5'\n_____________\|________________________________________\|\n_____________58_______________________________________25\n	133 -- 139	26 -- 32	-18.0428 kcal/mol	2.85625 kcal/mol	6.27605 kcal/mol	102 140@LB@ \| \|@LB@5'-AAA...ACUUC ACAAA A AUC C ACGAA...CGA-3'@LB@ ACUUGC UAA CAGGA AG CACU AAAAUUC@LB@ \|\|\|\|\|\| \|\|\| \|\|:\|\| \|\| \|\|\|\| \|\|\|\|\|\|\|@LB@ UGAACG AUU GUUCU UC GUGA UUUUAAG@LB@3'-UUU...UACUU A C AUU CAAUG...CAA-5'@LB@ \| \|@LB@ 58 25@LB@@LB@@LB@Energy -8.91 kcal/mol@LB@Hybridization Energy -18.0428 kcal/mol@LB@Unfolding Energy - mRNA 2.85625 kcal/mol@LB@Unfolding Energy - sRNA 6.27605 kcal/mol@LB@@LB@Position - mRNA 103 -- 139@LB@Position - sRNA 26 -- 57@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 26 -- 32@LB@
197	0.0237	0.3864	b4001	yjaH	-6.58	0.140477	149 -- 175	29 -- 57	DUF1481 family putative lipoprotein	_____________148____________________________176\n_____________\|______________________________\|\n5'-UGG...CCGGC________CU____________________CGCAG...AUA-3'\n______________AUUUGUUU__GGCA__AGGCACU_GAAAGA\n______________\|:\|\|\|:\|\|__:\|\|\|__\|:\|\|\|\|\|_:\|\|\|:\|\n______________UGAACGAA__UCGU__UUCGUGA_UUUUUU\n3'-UUU...UACUU________U_____UC_______A______AAGCA...CAA-5'\n_____________\|______________________________\|\n_____________58_____________________________28\n	163 -- 169	36 -- 42	-19.7163 kcal/mol	6.91297 kcal/mol	6.22375 kcal/mol	148 176@LB@ \| \|@LB@5'-UGG...CCGGC CU CGCAG...AUA-3'@LB@ AUUUGUUU GGCA AGGCACU GAAAGA@LB@ \|:\|\|\|:\|\| :\|\|\| \|:\|\|\|\|\| :\|\|\|:\|@LB@ UGAACGAA UCGU UUCGUGA UUUUUU@LB@3'-UUU...UACUU U UC A AAGCA...CAA-5'@LB@ \| \|@LB@ 58 28@LB@@LB@@LB@Energy -6.58 kcal/mol@LB@Hybridization Energy -19.7163 kcal/mol@LB@Unfolding Energy - mRNA 6.91297 kcal/mol@LB@Unfolding Energy - sRNA 6.22375 kcal/mol@LB@@LB@Position - mRNA 149 -- 175@LB@Position - sRNA 29 -- 57@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 36 -- 42@LB@
84	0.005596	0.2146	b4065	yjcE	-5.39	0.265673	197 -- 210	9 -- 22	putative cation/proton antiporter	_____________196____________211\n_____________\|______________\|\n5'-UAU...AGGGA____AU________UCUUC...UGC-3'\n______________ACGC__GGAAAUCU\n______________\|\|:\|__\|\|\|\|\|\|\|\|\n______________UGUG__CCUUUAGA\n3'-UUU...AGCAA____GU________CUACACAA-5'\n_____________\|______________\|\n_____________23_____________8\n	203 -- 209	10 -- 16	-15.8377 kcal/mol	2.89158 kcal/mol	7.5588 kcal/mol	196 211@LB@ \| \|@LB@5'-UAU...AGGGA AU UCUUC...UGC-3'@LB@ ACGC GGAAAUCU@LB@ \|\|:\| \|\|\|\|\|\|\|\|@LB@ UGUG CCUUUAGA@LB@3'-UUU...AGCAA GU CUACACAA-5'@LB@ \| \|@LB@ 23 8@LB@@LB@@LB@Energy -5.39 kcal/mol@LB@Hybridization Energy -15.8377 kcal/mol@LB@Unfolding Energy - mRNA 2.89158 kcal/mol@LB@Unfolding Energy - sRNA 7.5588 kcal/mol@LB@@LB@Position - mRNA 197 -- 210@LB@Position - sRNA 9 -- 22@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 10 -- 16@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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