Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
1	4.307e-10	1.387e-06	b2741	rpoS	-17.23	0.000044	75 -- 104	10 -- 40	RNA polymerase sigma S (sigma 38) factor	_____________74______________________________105\n_____________\|_______________________________\|\n5'-CGG...CGCUU______U______________AG________CGUAA...AGG-3'\n______________GCAUUU__GAAAUUCGUUACA__GGGAAAUC\n______________\|\|\|:\|\|__:\|\|\|\|\|\|\|\|\|\|\|\|__:\|\|\|\|\|\|\|\n______________CGUGAA__UUUUAAGCAAUGU__UCCUUUAG\n3'-UUU...UUCUU______UU_____________GG________ACUAC...CAA-5'\n_____________\|_______________________________\|\n_____________41______________________________9\n	98 -- 104	10 -- 16	-32.4258 kcal/mol	5.97429 kcal/mol	9.22048 kcal/mol	74 105@LB@ \| \|@LB@5'-CGG...CGCUU U AG CGUAA...AGG-3'@LB@ GCAUUU GAAAUUCGUUACA GGGAAAUC@LB@ \|\|\|:\|\| :\|\|\|\|\|\|\|\|\|\|\|\| :\|\|\|\|\|\|\|@LB@ CGUGAA UUUUAAGCAAUGU UCCUUUAG@LB@3'-UUU...UUCUU UU GG ACUAC...CAA-5'@LB@ \| \|@LB@ 41 9@LB@@LB@@LB@Energy -17.23 kcal/mol@LB@Hybridization Energy -32.4258 kcal/mol@LB@Unfolding Energy - mRNA 5.97429 kcal/mol@LB@Unfolding Energy - sRNA 9.22048 kcal/mol@LB@@LB@Position - mRNA 75 -- 104@LB@Position - sRNA 10 -- 40@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 10 -- 16@LB@
134	0.01281	0.308	b3316	rpsS	-8.71	0.038435	274 -- 288	39 -- 53	30S ribosomal subunit protein S19	_____________273______________289\n_____________\|________________\|\n5'-GCG...GGAAA__G____A________CCCUG...CUU-3'\n______________GC__GAG_CAAGAAGC\n______________\|\|__:\|\|_\|\|\|\|\|\|\|\|\n______________CG__UUC_GUUCUUCG\n3'-UUU...UUGAA__AA____________UGAAU...CAA-5'\n_____________\|________________\|\n_____________54_______________38\n	282 -- 288	39 -- 45	-16.1876 kcal/mol	1.46049 kcal/mol	6.01745 kcal/mol	273 289@LB@ \| \|@LB@5'-GCG...GGAAA G A CCCUG...CUU-3'@LB@ GC GAG CAAGAAGC@LB@ \|\| :\|\| \|\|\|\|\|\|\|\|@LB@ CG UUC GUUCUUCG@LB@3'-UUU...UUGAA AA UGAAU...CAA-5'@LB@ \| \|@LB@ 54 38@LB@@LB@@LB@Energy -8.71 kcal/mol@LB@Hybridization Energy -16.1876 kcal/mol@LB@Unfolding Energy - mRNA 1.46049 kcal/mol@LB@Unfolding Energy - sRNA 6.01745 kcal/mol@LB@@LB@Position - mRNA 274 -- 288@LB@Position - sRNA 39 -- 53@LB@Position Seed - mRNA 282 -- 288@LB@Position Seed - sRNA 39 -- 45@LB@
187	0.02188	0.3768	b3065	rpsU	-8.54	0.042773	188 -- 200	37 -- 49	30S ribosomal subunit protein S21	_____________187___________201\n_____________\|_____________\|\n5'-GAC...AUCAA_____________AUGCC...GUC-3'\n______________AGGUGAGAGGCAC\n______________\|:\|::\|\|\|:\|\|\|\|\n______________UUCGUUCUUCGUG\n3'-UUU...ACGAA_____________AAUUU...CAA-5'\n_____________\|_____________\|\n_____________50____________36\n	194 -- 200	37 -- 43	-18.635 kcal/mol	4.05763 kcal/mol	6.03369 kcal/mol	187 201@LB@ \| \|@LB@5'-GAC...AUCAA AUGCC...GUC-3'@LB@ AGGUGAGAGGCAC@LB@ \|:\|::\|\|\|:\|\|\|\|@LB@ UUCGUUCUUCGUG@LB@3'-UUU...ACGAA AAUUU...CAA-5'@LB@ \| \|@LB@ 50 36@LB@@LB@@LB@Energy -8.54 kcal/mol@LB@Hybridization Energy -18.635 kcal/mol@LB@Unfolding Energy - mRNA 4.05763 kcal/mol@LB@Unfolding Energy - sRNA 6.03369 kcal/mol@LB@@LB@Position - mRNA 188 -- 200@LB@Position - sRNA 37 -- 49@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 37 -- 43@LB@
32	0.0009536	0.09599	b0637	rsfS	-12.32	0.003012	197 -- 230	23 -- 59	ribosomal silencing factor	_____________196___________________________________231\n_____________\|_____________________________________\|\n5'-AUU...GGGGA________A_______________CC____U______AUCGA...UCA-3'\n______________AAACUUGC__GGGUA__AAGCACU__AGGA_UUUGUU\n______________\|\|\|\|\|\|\|\|__::\|:\|__\|\|\|\|\|\|\|__\|::\|_\|\|:\|\|\|\n______________UUUGAACG__UUCGU__UUCGUGA__UUUU_AAGCAA\n3'-UUU...CCUAC________AA_____UC_______AU____U______UGUGG...CAA-5'\n_____________\|_____________________________________\|\n_____________60____________________________________22\n	211 -- 217	36 -- 42	-25.7611 kcal/mol	7.16036 kcal/mol	6.27609 kcal/mol	196 231@LB@ \| \|@LB@5'-AUU...GGGGA A CC U AUCGA...UCA-3'@LB@ AAACUUGC GGGUA AAGCACU AGGA UUUGUU@LB@ \|\|\|\|\|\|\|\| ::\|:\| \|\|\|\|\|\|\| \|::\| \|\|:\|\|\|@LB@ UUUGAACG UUCGU UUCGUGA UUUU AAGCAA@LB@3'-UUU...CCUAC AA UC AU U UGUGG...CAA-5'@LB@ \| \|@LB@ 60 22@LB@@LB@@LB@Energy -12.32 kcal/mol@LB@Hybridization Energy -25.7611 kcal/mol@LB@Unfolding Energy - mRNA 7.16036 kcal/mol@LB@Unfolding Energy - sRNA 6.27609 kcal/mol@LB@@LB@Position - mRNA 197 -- 230@LB@Position - sRNA 23 -- 59@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 36 -- 42@LB@
72	0.003773	0.1688	b1860	ruvB	-7.81	0.067658	103 -- 111	38 -- 46	ATP-dependent DNA helicase component of RuvABC resolvasome	_____________102_______112\n_____________\|_________\|\n5'-ACG...AACCA_________AGCCG...GUC-3'\n______________CAAGAAGCA\n______________\|\|\|\|\|\|\|\|\|\n______________GUUCUUCGU\n3'-UUU...AAUUC_________GAAUU...CAA-5'\n_____________\|_________\|\n_____________47________37\n	105 -- 111	38 -- 44	-11.4188 kcal/mol	0.978333 kcal/mol	2.62609 kcal/mol	102 112@LB@ \| \|@LB@5'-ACG...AACCA AGCCG...GUC-3'@LB@ CAAGAAGCA@LB@ \|\|\|\|\|\|\|\|\|@LB@ GUUCUUCGU@LB@3'-UUU...AAUUC GAAUU...CAA-5'@LB@ \| \|@LB@ 47 37@LB@@LB@@LB@Energy -7.81 kcal/mol@LB@Hybridization Energy -11.4188 kcal/mol@LB@Unfolding Energy - mRNA 0.978333 kcal/mol@LB@Unfolding Energy - sRNA 2.62609 kcal/mol@LB@@LB@Position - mRNA 103 -- 111@LB@Position - sRNA 38 -- 46@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 38 -- 44@LB@
163	0.0182	0.3566	b0397	sbcC	-8.10	0.056577	196 -- 210	26 -- 43	exonuclease dsDNA ATP-dependent	_____________195________________211\n_____________\|__________________\|\n5'-GAA...AACAC__________________UCAGC...UUG-3'\n______________GAAGCAU___GAAAAUUC\n______________\|\|\|\|\|\|:___:\|\|\|\|\|\|\|\n______________CUUCGUG___UUUUUAAG\n3'-UUU...UCGUU_______AAU________CAAUG...CAA-5'\n_____________\|__________________\|\n_____________44_________________25\n	204 -- 210	26 -- 32	-13.6528 kcal/mol	1.79109 kcal/mol	3.75931 kcal/mol	195 211@LB@ \| \|@LB@5'-GAA...AACAC UCAGC...UUG-3'@LB@ GAAGCAU GAAAAUUC@LB@ \|\|\|\|\|\|: :\|\|\|\|\|\|\|@LB@ CUUCGUG UUUUUAAG@LB@3'-UUU...UCGUU AAU CAAUG...CAA-5'@LB@ \| \|@LB@ 44 25@LB@@LB@@LB@Energy -8.10 kcal/mol@LB@Hybridization Energy -13.6528 kcal/mol@LB@Unfolding Energy - mRNA 1.79109 kcal/mol@LB@Unfolding Energy - sRNA 3.75931 kcal/mol@LB@@LB@Position - mRNA 196 -- 210@LB@Position - sRNA 26 -- 43@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 26 -- 32@LB@
190	0.02239	0.3796	b0724	sdhB	-6.89	0.117799	143 -- 176	24 -- 60	succinate dehydrogenase FeS subunit	_____________142____________________________________177\n_____________\|______________________________________\|\n5'-UUU...GAACC_________GCCC____UUCC____C____________ACUUA...UGA-3'\n______________GAAAC_UGC____GGCA_____CGC___GAAGAUUCGU\n______________\|\|\|\|\|_\|\|\|____:\|\|\|_____\|:\|___:\|\|:\|\|\|\|\|\|\n______________CUUUG_ACG____UCGU_____GUG___UUUUUAAGCA\n3'-UUU...CCCUA_____A___AAU_____UCUUC___AAU__________AUGUG...CAA-5'\n_____________\|______________________________________\|\n_____________61_____________________________________23\n	170 -- 176	24 -- 30	-22.9017 kcal/mol	9.73835 kcal/mol	6.27609 kcal/mol	142 177@LB@ \| \|@LB@5'-UUU...GAACC GCCC UUCC C ACUUA...UGA-3'@LB@ GAAAC UGC GGCA CGC GAAGAUUCGU@LB@ \|\|\|\|\| \|\|\| :\|\|\| \|:\| :\|\|:\|\|\|\|\|\|@LB@ CUUUG ACG UCGU GUG UUUUUAAGCA@LB@3'-UUU...CCCUA A AAU UCUUC AAU AUGUG...CAA-5'@LB@ \| \|@LB@ 61 23@LB@@LB@@LB@Energy -6.89 kcal/mol@LB@Hybridization Energy -22.9017 kcal/mol@LB@Unfolding Energy - mRNA 9.73835 kcal/mol@LB@Unfolding Energy - sRNA 6.27609 kcal/mol@LB@@LB@Position - mRNA 143 -- 176@LB@Position - sRNA 24 -- 60@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 24 -- 30@LB@
13	0.0002543	0.06302	b3583	sgbE	-8.82	0.035786	171 -- 191	39 -- 61	L-ribulose-5-phosphate 4-epimerase	_____________170_____________________192\n_____________\|_______________________\|\n5'-CAA...UGGAU__________GUAU_________UGGAU...ACG-3'\n______________UGAAGC__GC____GCAGGAGGC\n______________\|\|\|\|:\|__\|\|____\|\|\|:\|\|:\|\|\n______________ACUUUG__CG____CGUUCUUCG\n3'-UUU...GCCCU______AA__AAUU_________UGAAU...CAA-5'\n_____________\|_______________________\|\n_____________62______________________38\n	185 -- 191	39 -- 45	-21.2799 kcal/mol	5.20492 kcal/mol	7.25518 kcal/mol	170 192@LB@ \| \|@LB@5'-CAA...UGGAU GUAU UGGAU...ACG-3'@LB@ UGAAGC GC GCAGGAGGC@LB@ \|\|\|\|:\| \|\| \|\|\|:\|\|:\|\|@LB@ ACUUUG CG CGUUCUUCG@LB@3'-UUU...GCCCU AA AAUU UGAAU...CAA-5'@LB@ \| \|@LB@ 62 38@LB@@LB@@LB@Energy -8.82 kcal/mol@LB@Hybridization Energy -21.2799 kcal/mol@LB@Unfolding Energy - mRNA 5.20492 kcal/mol@LB@Unfolding Energy - sRNA 7.25518 kcal/mol@LB@@LB@Position - mRNA 171 -- 191@LB@Position - sRNA 39 -- 61@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 39 -- 45@LB@
165	0.01827	0.3566	b2937	speB	-7.30	0.092452	5 -- 22	25 -- 41	agmatinase	_____________4__________________23\n_____________\|__________________\|\n_______5'-CAAC___UC_____________AGGCA...CAG-3'\n______________AGU__CUUGAAGAGUUCG\n______________\|\|:__\|\|\|:\|\|:\|:\|\|\|\|\n______________UCG__GAAUUUUUUAAGC\n3'-UUU...GUUCU___U______________AAUGU...CAA-5'\n_____________\|__________________\|\n_____________42_________________24\n	16 -- 22	25 -- 31	-14.9284 kcal/mol	5.98061 kcal/mol	1.64839 kcal/mol	4 23@LB@ \| \|@LB@ 5'-CAAC UC AGGCA...CAG-3'@LB@ AGU CUUGAAGAGUUCG@LB@ \|\|: \|\|\|:\|\|:\|:\|\|\|\|@LB@ UCG GAAUUUUUUAAGC@LB@3'-UUU...GUUCU U AAUGU...CAA-5'@LB@ \| \|@LB@ 42 24@LB@@LB@@LB@Energy -7.30 kcal/mol@LB@Hybridization Energy -14.9284 kcal/mol@LB@Unfolding Energy - mRNA 5.98061 kcal/mol@LB@Unfolding Energy - sRNA 1.64839 kcal/mol@LB@@LB@Position - mRNA 5 -- 22@LB@Position - sRNA 25 -- 41@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 25 -- 31@LB@
68	0.003533	0.1645	b3650	spoT	-9.27	0.026581	116 -- 140	36 -- 60	bifunctional (p)ppGpp synthetase II/ guanosine-3' 5'-bis pyrophosphate 3'-pyrophosphohydrolase	_____________115________________________141\n_____________\|__________________________\|\n5'-UAG...CGCCA_____A___AA___________C___GAAUU...CUC-3'\n______________GGAAC__GC__GAGCAGGAAGC_GCU\n______________\|:\|\|\|__\|\|__:\|\|\|\|:\|\|\|\|\|_:\|\|\n______________CUUUG__CG__UUCGUUCUUCG_UGA\n3'-UUU...CCCUA_____AA__AA_______________AUUUU...CAA-5'\n_____________\|__________________________\|\n_____________61_________________________35\n	130 -- 136	39 -- 45	-24.6059 kcal/mol	9.08429 kcal/mol	6.24973 kcal/mol	115 141@LB@ \| \|@LB@5'-UAG...CGCCA A AA C GAAUU...CUC-3'@LB@ GGAAC GC GAGCAGGAAGC GCU@LB@ \|:\|\|\| \|\| :\|\|\|\|:\|\|\|\|\| :\|\|@LB@ CUUUG CG UUCGUUCUUCG UGA@LB@3'-UUU...CCCUA AA AA AUUUU...CAA-5'@LB@ \| \|@LB@ 61 35@LB@@LB@@LB@Energy -9.27 kcal/mol@LB@Hybridization Energy -24.6059 kcal/mol@LB@Unfolding Energy - mRNA 9.08429 kcal/mol@LB@Unfolding Energy - sRNA 6.24973 kcal/mol@LB@@LB@Position - mRNA 116 -- 140@LB@Position - sRNA 36 -- 60@LB@Position Seed - mRNA 130 -- 136@LB@Position Seed - sRNA 39 -- 45@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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