Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
17	5.719e-05	0.01183	b3675	yidG	-12.05	0.010096	82 -- 130	1 -- 56	inner membrane protein		_____________81_____________________________________________________________131\n_____________\|______________________________________________________________\|\n5'-CGC...UUGCG_________CAAU_A_____CGAUGC__________GG________________________ACCAA...CGU-3'\n______________CUGGUUACG____G_AAAAG______GUCUGAAAGA___________ACCU____GCCCUAC\n______________\|:\|:\|\|\|\|\|____\|_\|\|\|\|\|______\|\|\|\|\|\|\|\|\|\|___________\|\|\|\|____:\|\|\|\|\|\|\n______________GGCUAAUGC____C_UUUUC______CAGACUUUCU___________UGGA____UGGGAUG\n3'-AUU...GUUUA_________ACUU_A_____________________AUCUUGUAGAA____GACA_______-5'\n_____________\|_____________________________________________________________\|\n_____________57____________________________________________________________1\n	124 -- 130	1 -- 7	-38.348 kcal/mol	13.6157 kcal/mol	12.7022 kcal/mol	81 131@LB@ \| \|@LB@5'-CGC...UUGCG CAAU A CGAUGC GG ACCAA...CGU-3'@LB@ CUGGUUACG G AAAAG GUCUGAAAGA ACCU GCCCUAC@LB@ \|:\|:\|\|\|\|\| \| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\| :\|\|\|\|\|\|@LB@ GGCUAAUGC C UUUUC CAGACUUUCU UGGA UGGGAUG@LB@3'-AUU...GUUUA ACUU A AUCUUGUAGAA GACA -5'@LB@ \| \|@LB@ 57 1@LB@@LB@@LB@Energy -12.05 kcal/mol@LB@Hybridization Energy -38.348 kcal/mol@LB@Unfolding Energy - mRNA 13.6157 kcal/mol@LB@Unfolding Energy - sRNA 12.7022 kcal/mol@LB@@LB@Position - mRNA 82 -- 130@LB@Position - sRNA 1 -- 56@LB@Position Seed - mRNA 124 -- 130@LB@Position Seed - sRNA 1 -- 7@LB@
41	0.000458	0.03766	b3679	yidK	-16.91	0.000078	119 -- 159	1 -- 60	putative transporter		_____________118__________________________________________________________160\n_____________\|____________________________________________________________\|\n5'-UUU...UUUAA________C_____________________CCCGAC________________________AUUCA...AAG-3'\n______________GAAUCUGA_UGC______________CUGA________GCAUUUUACCUCU___CCCUAU\n______________:\|\|\|\|:\|\|_\|:\|______________\|\|\|\|________:\|\|\|:\|\|\|\|\|\|\|\|___\|\|\|\|\|:\n______________UUUAGGCU_AUG______________GACU________UGUAGAAUGGAGA___GGGAUG\n3'-AUU...GUCGG________A___CACUUCAUUUUCCA____UUCUAUCU_____________CAU______-5'\n_____________\|___________________________________________________________\|\n_____________61__________________________________________________________1\n	147 -- 153	10 -- 16	-34.139 kcal/mol	4.55251 kcal/mol	12.7042 kcal/mol	118 160@LB@ \| \|@LB@5'-UUU...UUUAA C CCCGAC AUUCA...AAG-3'@LB@ GAAUCUGA UGC CUGA GCAUUUUACCUCU CCCUAU@LB@ :\|\|\|\|:\|\| \|:\| \|\|\|\| :\|\|\|:\|\|\|\|\|\|\|\| \|\|\|\|\|:@LB@ UUUAGGCU AUG GACU UGUAGAAUGGAGA GGGAUG@LB@3'-AUU...GUCGG A CACUUCAUUUUCCA UUCUAUCU CAU -5'@LB@ \| \|@LB@ 61 1@LB@@LB@@LB@Energy -16.91 kcal/mol@LB@Hybridization Energy -34.139 kcal/mol@LB@Unfolding Energy - mRNA 4.55251 kcal/mol@LB@Unfolding Energy - sRNA 12.7042 kcal/mol@LB@@LB@Position - mRNA 119 -- 159@LB@Position - sRNA 1 -- 60@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 10 -- 16@LB@
50	0.0006038	0.04249	b3755	yieP	-5.20	0.556147	189 -- 196	25 -- 32	putative transcriptional regulator		_____________188______197\n_____________\|________\|\n5'-UUC...AAAAA________AGUAA...CCG-3'\n______________GGAAGAUA\n______________\|:\|\|\|\|\|\|\n______________CUUUCUAU\n3'-AUU...CCAGA________CUUGU...AUG-5'\n_____________\|________\|\n_____________33_______24\n	190 -- 196	25 -- 31	-8.32385 kcal/mol	1.19727 kcal/mol	1.85262 kcal/mol	188 197@LB@ \| \|@LB@5'-UUC...AAAAA AGUAA...CCG-3'@LB@ GGAAGAUA@LB@ \|:\|\|\|\|\|\|@LB@ CUUUCUAU@LB@3'-AUU...CCAGA CUUGU...AUG-5'@LB@ \| \|@LB@ 33 24@LB@@LB@@LB@Energy -5.20 kcal/mol@LB@Hybridization Energy -8.32385 kcal/mol@LB@Unfolding Energy - mRNA 1.19727 kcal/mol@LB@Unfolding Energy - sRNA 1.85262 kcal/mol@LB@@LB@Position - mRNA 189 -- 196@LB@Position - sRNA 25 -- 32@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 25 -- 31@LB@
118	0.003907	0.1165	b3827	yigM	-8.32	0.129405	260 -- 268	1 -- 9	putative inner membrane EamA-like transporter		_____________259_______269\n_____________\|_________\|\n5'-GGA...GGCGA_________GGGGC...UGG-3'\n______________GUACCUUGC\n______________\|\|\|\|\|:\|:\|\n______________CAUGGGAUG\n3'-AUU...GGAGA_________-5'\n_____________\|________\|\n_____________10_______1\n	262 -- 268	1 -- 7	-12.9582 kcal/mol	2.53078 kcal/mol	2.12872 kcal/mol	259 269@LB@ \| \|@LB@5'-GGA...GGCGA GGGGC...UGG-3'@LB@ GUACCUUGC@LB@ \|\|\|\|\|:\|:\|@LB@ CAUGGGAUG@LB@3'-AUU...GGAGA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -8.32 kcal/mol@LB@Hybridization Energy -12.9582 kcal/mol@LB@Unfolding Energy - mRNA 2.53078 kcal/mol@LB@Unfolding Energy - sRNA 2.12872 kcal/mol@LB@@LB@Position - mRNA 260 -- 268@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 262 -- 268@LB@Position Seed - sRNA 1 -- 7@LB@
112	0.003594	0.1129	b3866	yihI	-3.87	0.795256	171 -- 194	53 -- 75	activator of Der GTPase		_____________170______________________195\n_____________\|________________________\|\n5'-GAA...CUAUA_____UAAAA________AGA___ACCAU...GUC-3'\n______________CUGAA_____CGGCUAAA___CGA\n______________\|\|:\|\|_____\|:\|\|:\|\|\|___\|\|\|\n______________GAUUU_____GUCGGUUU___GCU\n3'-AUU...CCGCC_____UAUAA________AG____AAUGC...AUG-5'\n_____________\|________________________\|\n_____________76_______________________52\n	181 -- 187	59 -- 65	-14.4152 kcal/mol	4.20142 kcal/mol	6.36326 kcal/mol	170 195@LB@ \| \|@LB@5'-GAA...CUAUA UAAAA AGA ACCAU...GUC-3'@LB@ CUGAA CGGCUAAA CGA@LB@ \|\|:\|\| \|:\|\|:\|\|\| \|\|\|@LB@ GAUUU GUCGGUUU GCU@LB@3'-AUU...CCGCC UAUAA AG AAUGC...AUG-5'@LB@ \| \|@LB@ 76 52@LB@@LB@@LB@Energy -3.87 kcal/mol@LB@Hybridization Energy -14.4152 kcal/mol@LB@Unfolding Energy - mRNA 4.20142 kcal/mol@LB@Unfolding Energy - sRNA 6.36326 kcal/mol@LB@@LB@Position - mRNA 171 -- 194@LB@Position - sRNA 53 -- 75@LB@Position Seed - mRNA 181 -- 187@LB@Position Seed - sRNA 59 -- 65@LB@
88	0.002339	0.09349	b3878	yihQ	-11.00	0.022508	260 -- 267	1 -- 8	putative sulpholipid alpha-glucosidase; alpha-glucosyl fluoride glucosidase	b3656	_____________259______268\n_____________\|________\|\n5'-AAC...AGUUU________AUUUU...GCA-3'\n______________UACCCUAC\n______________\|\|\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	261 -- 267	1 -- 7	-13.427 kcal/mol	0.33456 kcal/mol	2.11603 kcal/mol	259 268@LB@ \| \|@LB@5'-AAC...AGUUU AUUUU...GCA-3'@LB@ UACCCUAC@LB@ \|\|\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -11.00 kcal/mol@LB@Hybridization Energy -13.427 kcal/mol@LB@Unfolding Energy - mRNA 0.33456 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 260 -- 267@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 261 -- 267@LB@Position Seed - sRNA 1 -- 7@LB@
52	0.0006736	0.04557	b3964	yijD	-9.23	0.074829	177 -- 192	43 -- 57	DUF1422 family inner membrane protein		_____________176______________193\n_____________\|________________\|\n5'-ACU...AUUAU_____GAA________GACGA...UGC-3'\n______________UCCGG___AUGUGAAG\n______________\|\|\|\|:___\|:\|\|\|\|\|\|\n______________AGGCU___UGCACUUC\n3'-AUU...GGUUU_____AA_________AUUUU...AUG-5'\n_____________\|________________\|\n_____________58_______________42\n	186 -- 192	43 -- 49	-16.7307 kcal/mol	4.24116 kcal/mol	3.27382 kcal/mol	176 193@LB@ \| \|@LB@5'-ACU...AUUAU GAA GACGA...UGC-3'@LB@ UCCGG AUGUGAAG@LB@ \|\|\|\|: \|:\|\|\|\|\|\|@LB@ AGGCU UGCACUUC@LB@3'-AUU...GGUUU AA AUUUU...AUG-5'@LB@ \| \|@LB@ 58 42@LB@@LB@@LB@Energy -9.23 kcal/mol@LB@Hybridization Energy -16.7307 kcal/mol@LB@Unfolding Energy - mRNA 4.24116 kcal/mol@LB@Unfolding Energy - sRNA 3.27382 kcal/mol@LB@@LB@Position - mRNA 177 -- 192@LB@Position - sRNA 43 -- 57@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 43 -- 49@LB@
178	0.007695	0.1511	b4567	yjjZ	-7.25	0.231371	185 -- 218	2 -- 33	uncharacterized protein		_____________184_________________________________219\n_____________\|___________________________________\|\n5'-CGG...AUCAA_______GAU___AU___G____AA______G___GGCAG...GGA-3'\n______________UGAAAGA___GAA__CAU_UUGC__C_GUAC_CUG\n______________\|\|\|\|\|\|\|___\|\|\|__\|\|\|_\|\|:\|__\|_\|\|\|\|_\|\|:\n______________ACUUUCU___CUU__GUA_AAUG__G_CAUG_GAU\n3'-AUU...UCCAG_______AU_________G____GA_A____G___G-5'\n_____________\|___________________________________\|\n_____________34__________________________________1\n	185 -- 191	27 -- 33	-22.8177 kcal/mol	6.54395 kcal/mol	9.04499 kcal/mol	184 219@LB@ \| \|@LB@5'-CGG...AUCAA GAU AU G AA G GGCAG...GGA-3'@LB@ UGAAAGA GAA CAU UUGC C GUAC CUG@LB@ \|\|\|\|\|\|\| \|\|\| \|\|\| \|\|:\| \| \|\|\|\| \|\|:@LB@ ACUUUCU CUU GUA AAUG G CAUG GAU@LB@3'-AUU...UCCAG AU G GA A G G-5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -7.25 kcal/mol@LB@Hybridization Energy -22.8177 kcal/mol@LB@Unfolding Energy - mRNA 6.54395 kcal/mol@LB@Unfolding Energy - sRNA 9.04499 kcal/mol@LB@@LB@Position - mRNA 185 -- 218@LB@Position - sRNA 2 -- 33@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 27 -- 33@LB@
103	0.003241	0.1101	b0837	yliI	-8.40	0.123505	223 -- 230	4 -- 11	soluble aldose sugar dehydrogenase		_____________222______231\n_____________\|________\|\n5'-GUC...UUUCC________CUUAU...AAC-3'\n______________UUGUGCCC\n______________:\|\|\|:\|\|\|\n______________GACAUGGG\n3'-AUU...AUGGA________AUG-5'\n_____________\|________\|\n_____________12_______3\n	224 -- 230	4 -- 10	-12.324 kcal/mol	0.491988 kcal/mol	3.45361 kcal/mol	222 231@LB@ \| \|@LB@5'-GUC...UUUCC CUUAU...AAC-3'@LB@ UUGUGCCC@LB@ :\|\|\|:\|\|\|@LB@ GACAUGGG@LB@3'-AUU...AUGGA AUG-5'@LB@ \| \|@LB@ 12 3@LB@@LB@@LB@Energy -8.40 kcal/mol@LB@Hybridization Energy -12.324 kcal/mol@LB@Unfolding Energy - mRNA 0.491988 kcal/mol@LB@Unfolding Energy - sRNA 3.45361 kcal/mol@LB@@LB@Position - mRNA 223 -- 230@LB@Position - sRNA 4 -- 11@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 4 -- 10@LB@
168	0.006917	0.1448	b3070	yqjH	-8.25	0.134865	172 -- 181	49 -- 58	putative siderophore interacting protein		_____________171________182\n_____________\|__________\|\n5'-ACU...GAUAU__________CAUCA...AGC-3'\n______________AUCUGAUUAC\n______________\|\|\|:\|\|\|\|\|\|\n______________UAGGCUAAUG\n3'-AUU...CGGUU__________CACUU...AUG-5'\n_____________\|__________\|\n_____________59_________48\n	175 -- 181	49 -- 55	-10.8722 kcal/mol	2.31397 kcal/mol	0.318367 kcal/mol	171 182@LB@ \| \|@LB@5'-ACU...GAUAU CAUCA...AGC-3'@LB@ AUCUGAUUAC@LB@ \|\|\|:\|\|\|\|\|\|@LB@ UAGGCUAAUG@LB@3'-AUU...CGGUU CACUU...AUG-5'@LB@ \| \|@LB@ 59 48@LB@@LB@@LB@Energy -8.25 kcal/mol@LB@Hybridization Energy -10.8722 kcal/mol@LB@Unfolding Energy - mRNA 2.31397 kcal/mol@LB@Unfolding Energy - sRNA 0.318367 kcal/mol@LB@@LB@Position - mRNA 172 -- 181@LB@Position - sRNA 49 -- 58@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 49 -- 55@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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