Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
105	0.003348	0.1101	b2218	rcsC	-11.03	0.021992	174 -- 214	1 -- 37	hybrid sensory kinase in two-component regulatory system with RcsB and YojN	_____________173_______________________________________215\n_____________\|_________________________________________\|\n5'-AGC...CCAUC____ACCU____C_____U__GCC______AAA________UUCUU...CCG-3'\n______________GGUC____GAGG_GGAGC_UC___CCUUUG___UACCUUGC\n______________\|\|\|\|____:\|\|:_:\|\|:\|_\|\|___\|\|\|:\|\|___\|\|\|\|:\|:\|\n______________CCAG____UUCU_UCUUG_AG___GGAGAC___AUGGGAUG\n3'-AUU...AUUUU____ACU_____A_____U__AAU_________________-5'\n_____________\|________________________________________\|\n_____________38_______________________________________1\n	208 -- 214	1 -- 7	-33.3544 kcal/mol	10.9963 kcal/mol	11.3499 kcal/mol	173 215@LB@ \| \|@LB@5'-AGC...CCAUC ACCU C U GCC AAA UUCUU...CCG-3'@LB@ GGUC GAGG GGAGC UC CCUUUG UACCUUGC@LB@ \|\|\|\| :\|\|: :\|\|:\| \|\| \|\|\|:\|\| \|\|\|\|:\|:\|@LB@ CCAG UUCU UCUUG AG GGAGAC AUGGGAUG@LB@3'-AUU...AUUUU ACU A U AAU -5'@LB@ \| \|@LB@ 38 1@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -33.3544 kcal/mol@LB@Unfolding Energy - mRNA 10.9963 kcal/mol@LB@Unfolding Energy - sRNA 11.3499 kcal/mol@LB@@LB@Position - mRNA 174 -- 214@LB@Position - sRNA 1 -- 37@LB@Position Seed - mRNA 208 -- 214@LB@Position Seed - sRNA 1 -- 7@LB@
149	0.005967	0.1406	b0472	recR	-13.66	0.002580	134 -- 160	3 -- 29	gap repair protein	_____________133_________________________161\n_____________\|___________________________\|\n5'-GUG...AACGC___A____AAAAUG_____________CCGGA...UUC-3'\n______________AGA_AGAA______GCCUCUGUAUCCU\n______________\|\|\|_\|\|\|\|______:\|\|\|\|\|\|\|\|:\|\|\|\n______________UCU_UCUU______UGGAGACAUGGGA\n3'-AUU...GACUU___A____GUAGAA_____________UG-5'\n_____________\|___________________________\|\n_____________30__________________________2\n	154 -- 160	3 -- 9	-27.6106 kcal/mol	5.92472 kcal/mol	8.05021 kcal/mol	133 161@LB@ \| \|@LB@5'-GUG...AACGC A AAAAUG CCGGA...UUC-3'@LB@ AGA AGAA GCCUCUGUAUCCU@LB@ \|\|\| \|\|\|\| :\|\|\|\|\|\|\|\|:\|\|\|@LB@ UCU UCUU UGGAGACAUGGGA@LB@3'-AUU...GACUU A GUAGAA UG-5'@LB@ \| \|@LB@ 30 2@LB@@LB@@LB@Energy -13.66 kcal/mol@LB@Hybridization Energy -27.6106 kcal/mol@LB@Unfolding Energy - mRNA 5.92472 kcal/mol@LB@Unfolding Energy - sRNA 8.05021 kcal/mol@LB@@LB@Position - mRNA 134 -- 160@LB@Position - sRNA 3 -- 29@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 3 -- 9@LB@
30	0.0002612	0.03063	b3789	rffH	-9.15	0.078548	244 -- 254	48 -- 58	glucose-1-phosphate thymidylyltransferase	_____________243_________255\n_____________\|___________\|\n5'-GGC...AUUGC___________CGCGG...UGA-3'\n______________AUCCGAUUACG\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________UAGGCUAAUGC\n3'-AUU...CGGUU___________ACUUC...AUG-5'\n_____________\|___________\|\n_____________59__________47\n	248 -- 254	48 -- 54	-15.7126 kcal/mol	4.98099 kcal/mol	1.55987 kcal/mol	243 255@LB@ \| \|@LB@5'-GGC...AUUGC CGCGG...UGA-3'@LB@ AUCCGAUUACG@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ UAGGCUAAUGC@LB@3'-AUU...CGGUU ACUUC...AUG-5'@LB@ \| \|@LB@ 59 47@LB@@LB@@LB@Energy -9.15 kcal/mol@LB@Hybridization Energy -15.7126 kcal/mol@LB@Unfolding Energy - mRNA 4.98099 kcal/mol@LB@Unfolding Energy - sRNA 1.55987 kcal/mol@LB@@LB@Position - mRNA 244 -- 254@LB@Position - sRNA 48 -- 58@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 48 -- 54@LB@
101	0.00315	0.1097	b1822	rlmA	-7.85	0.169074	182 -- 190	1 -- 9	23S rRNA m(1)G745 methyltransferase SAM-dependent	_____________181_______191\n_____________\|_________\|\n5'-AAG...CUUUA_________CGGAG...GGU-3'\n______________GUAUCCUGC\n______________\|\|\|:\|\|\|:\|\n______________CAUGGGAUG\n3'-AUU...GGAGA_________-5'\n_____________\|________\|\n_____________10_______1\n	184 -- 190	1 -- 7	-13.1594 kcal/mol	3.20778 kcal/mol	2.12872 kcal/mol	181 191@LB@ \| \|@LB@5'-AAG...CUUUA CGGAG...GGU-3'@LB@ GUAUCCUGC@LB@ \|\|\|:\|\|\|:\|@LB@ CAUGGGAUG@LB@3'-AUU...GGAGA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -7.85 kcal/mol@LB@Hybridization Energy -13.1594 kcal/mol@LB@Unfolding Energy - mRNA 3.20778 kcal/mol@LB@Unfolding Energy - sRNA 2.12872 kcal/mol@LB@@LB@Position - mRNA 182 -- 190@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 1 -- 7@LB@
144	0.005273	0.1288	b1652	rnt	-8.26	0.134019	203 -- 213	48 -- 58	RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair	_____________202_________214\n_____________\|___________\|\n5'-AAG...CAGAU_______A___CUCAA...CCG-3'\n______________GUCCGAU_ACG\n______________:\|\|\|\|\|\|_\|\|\|\n______________UAGGCUA_UGC\n3'-AUU...CGGUU_______A___ACUUC...AUG-5'\n_____________\|___________\|\n_____________59__________47\n	203 -- 209	52 -- 58	-12.2442 kcal/mol	2.4043 kcal/mol	1.55987 kcal/mol	202 214@LB@ \| \|@LB@5'-AAG...CAGAU A CUCAA...CCG-3'@LB@ GUCCGAU ACG@LB@ :\|\|\|\|\|\| \|\|\|@LB@ UAGGCUA UGC@LB@3'-AUU...CGGUU A ACUUC...AUG-5'@LB@ \| \|@LB@ 59 47@LB@@LB@@LB@Energy -8.26 kcal/mol@LB@Hybridization Energy -12.2442 kcal/mol@LB@Unfolding Energy - mRNA 2.4043 kcal/mol@LB@Unfolding Energy - sRNA 1.55987 kcal/mol@LB@@LB@Position - mRNA 203 -- 213@LB@Position - sRNA 48 -- 58@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 52 -- 58@LB@
195	0.008707	0.1558	b3318	rplW	-7.58	0.194751	202 -- 213	43 -- 55	50S ribosomal subunit protein L23	_____________201___________214\n_____________\|_____________\|\n5'-GCA...UGGCA_____________AACGU...AAG-3'\n______________UGAUU_CGUGAAG\n______________:\|\|\|\|_\|\|\|\|\|\|\|\n______________GCUAA_GCACUUC\n3'-AUU...UUUAG_____U_______AUUUU...AUG-5'\n_____________\|_____________\|\n_____________56____________42\n	207 -- 213	43 -- 49	-13.4464 kcal/mol	2.60751 kcal/mol	3.2693 kcal/mol	201 214@LB@ \| \|@LB@5'-GCA...UGGCA AACGU...AAG-3'@LB@ UGAUU CGUGAAG@LB@ :\|\|\|\| \|\|\|\|\|\|\|@LB@ GCUAA GCACUUC@LB@3'-AUU...UUUAG U AUUUU...AUG-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -7.58 kcal/mol@LB@Hybridization Energy -13.4464 kcal/mol@LB@Unfolding Energy - mRNA 2.60751 kcal/mol@LB@Unfolding Energy - sRNA 3.2693 kcal/mol@LB@@LB@Position - mRNA 202 -- 213@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 207 -- 213@LB@Position Seed - sRNA 43 -- 49@LB@
42	0.0004615	0.03766	b3341	rpsG	-11.13	0.020425	227 -- 249	53 -- 76	30S ribosomal subunit protein S7	_____________226______________________250\n_____________\|________________________\|\n5'-CGG...GGUCA__GU________U___________AGUUC...AUG-3'\n______________GC__AAA_AUUC_GCCGGAUCCGA\n______________\|\|__\|\|\|_\|\|\|\|_\|\|\|::\|\|\|\|\|\|\n______________CG__UUU_UAAG_CGGUUUAGGCU\n3'-AUU...CCCGC__AU___A____U___________AAUGC...AUG-5'\n_____________\|________________________\|\n_____________77_______________________52\n	243 -- 249	53 -- 59	-23.8099 kcal/mol	6.33566 kcal/mol	6.36489 kcal/mol	226 250@LB@ \| \|@LB@5'-CGG...GGUCA GU U AGUUC...AUG-3'@LB@ GC AAA AUUC GCCGGAUCCGA@LB@ \|\| \|\|\| \|\|\|\| \|\|\|::\|\|\|\|\|\|@LB@ CG UUU UAAG CGGUUUAGGCU@LB@3'-AUU...CCCGC AU A U AAUGC...AUG-5'@LB@ \| \|@LB@ 77 52@LB@@LB@@LB@Energy -11.13 kcal/mol@LB@Hybridization Energy -23.8099 kcal/mol@LB@Unfolding Energy - mRNA 6.33566 kcal/mol@LB@Unfolding Energy - sRNA 6.36489 kcal/mol@LB@@LB@Position - mRNA 227 -- 249@LB@Position - sRNA 53 -- 76@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 53 -- 59@LB@
81	0.001761	0.07647	b3311	rpsQ	-9.62	0.058411	217 -- 225	1 -- 9	30S ribosomal subunit protein S17	_____________216_______226\n_____________\|_________\|\n5'-GGU...AAUCC_________AAGGU...UCU-3'\n______________GUACUCUGC\n______________\|\|\|\|:\|\|:\|\n______________CAUGGGAUG\n3'-AUU...GGAGA_________-5'\n_____________\|________\|\n_____________10_______1\n	219 -- 225	1 -- 7	-13.7932 kcal/mol	2.07071 kcal/mol	2.12872 kcal/mol	216 226@LB@ \| \|@LB@5'-GGU...AAUCC AAGGU...UCU-3'@LB@ GUACUCUGC@LB@ \|\|\|\|:\|\|:\|@LB@ CAUGGGAUG@LB@3'-AUU...GGAGA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -9.62 kcal/mol@LB@Hybridization Energy -13.7932 kcal/mol@LB@Unfolding Energy - mRNA 2.07071 kcal/mol@LB@Unfolding Energy - sRNA 2.12872 kcal/mol@LB@@LB@Position - mRNA 217 -- 225@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 219 -- 225@LB@Position Seed - sRNA 1 -- 7@LB@
184	0.00812	0.1553	b4371	rsmC	-7.50	0.203534	191 -- 216	4 -- 25	16S rRNA m(2)G1207 methyltransferase SAM-dependent	_____________190________________________217\n_____________\|__________________________\|\n5'-AUC...AUUGA________CC__G____CAUU_____CGGCA...UGC-3'\n______________GGAGCAUU__AU_UCUG____UACCC\n______________:\|\|:\|\|\|:__\|:_\|\|\|\|____\|\|\|\|\|\n______________UCUUGUAG__UG_AGAC____AUGGG\n3'-AUU...UUCUA________AA__G_____________AUG-5'\n_____________\|__________________________\|\n_____________26_________________________3\n	192 -- 198	18 -- 24	-20.5319 kcal/mol	5.03265 kcal/mol	8.0209 kcal/mol	190 217@LB@ \| \|@LB@5'-AUC...AUUGA CC G CAUU CGGCA...UGC-3'@LB@ GGAGCAUU AU UCUG UACCC@LB@ :\|\|:\|\|\|: \|: \|\|\|\| \|\|\|\|\|@LB@ UCUUGUAG UG AGAC AUGGG@LB@3'-AUU...UUCUA AA G AUG-5'@LB@ \| \|@LB@ 26 3@LB@@LB@@LB@Energy -7.50 kcal/mol@LB@Hybridization Energy -20.5319 kcal/mol@LB@Unfolding Energy - mRNA 5.03265 kcal/mol@LB@Unfolding Energy - sRNA 8.0209 kcal/mol@LB@@LB@Position - mRNA 191 -- 216@LB@Position - sRNA 4 -- 25@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 18 -- 24@LB@
76	0.001511	0.06993	b3465	rsmD	-10.43	0.033857	227 -- 244	49 -- 65	16S rRNA m(2)G966 methyltransferase SAM-dependent	_____________226________________245\n_____________\|__________________\|\n5'-UUG...GGCAG____________C_____UGGCG...GCC-3'\n______________CGGCCAAAUCCG_AUUAU\n______________\|:\|\|\|\|\|\|\|\|\|\|_\|\|\|\|:\n______________GUCGGUUUAGGC_UAAUG\n3'-AUU...UAUAA__________________CACUU...AUG-5'\n_____________\|__________________\|\n_____________66_________________48\n	232 -- 238	54 -- 60	-22.129 kcal/mol	5.5281 kcal/mol	6.19707 kcal/mol	226 245@LB@ \| \|@LB@5'-UUG...GGCAG C UGGCG...GCC-3'@LB@ CGGCCAAAUCCG AUUAU@LB@ \|:\|\|\|\|\|\|\|\|\|\| \|\|\|\|:@LB@ GUCGGUUUAGGC UAAUG@LB@3'-AUU...UAUAA CACUU...AUG-5'@LB@ \| \|@LB@ 66 48@LB@@LB@@LB@Energy -10.43 kcal/mol@LB@Hybridization Energy -22.129 kcal/mol@LB@Unfolding Energy - mRNA 5.5281 kcal/mol@LB@Unfolding Energy - sRNA 6.19707 kcal/mol@LB@@LB@Position - mRNA 227 -- 244@LB@Position - sRNA 49 -- 65@LB@Position Seed - mRNA 232 -- 238@LB@Position Seed - sRNA 54 -- 60@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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