Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
67	0.001147	0.05856	b2319	usg	-10.96	0.023185	203 -- 221	18 -- 36	putative semialdehyde dehydrogenase	_____________202_________________222\n_____________\|___________________\|\n5'-GAG...ACCAU_________C_________GCCGU...AAA-3'\n______________GUCUGAAGG_UGGAACAUU\n______________\|\|\|\|\|\|\|:\|_\|:\|\|\|\|\|\|:\n______________CAGACUUUC_AUCUUGUAG\n3'-AUU...UUUUC_________U_________AAUGG...AUG-5'\n_____________\|___________________\|\n_____________37__________________17\n	215 -- 221	18 -- 24	-22.956 kcal/mol	5.32254 kcal/mol	6.60045 kcal/mol	202 222@LB@ \| \|@LB@5'-GAG...ACCAU C GCCGU...AAA-3'@LB@ GUCUGAAGG UGGAACAUU@LB@ \|\|\|\|\|\|\|:\| \|:\|\|\|\|\|\|:@LB@ CAGACUUUC AUCUUGUAG@LB@3'-AUU...UUUUC U AAUGG...AUG-5'@LB@ \| \|@LB@ 37 17@LB@@LB@@LB@Energy -10.96 kcal/mol@LB@Hybridization Energy -22.956 kcal/mol@LB@Unfolding Energy - mRNA 5.32254 kcal/mol@LB@Unfolding Energy - sRNA 6.60045 kcal/mol@LB@@LB@Position - mRNA 203 -- 221@LB@Position - sRNA 18 -- 36@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 18 -- 24@LB@
38	0.0004278	0.03766	b3092	uxaC	-12.53	0.006845	206 -- 269	1 -- 57	uronate isomerase	_____________205_________________________________________________________________270\n_____________\|___________________________________________________________________\|\n5'-UUG...AUGAC____U_____AC_____AUUUC______UU_____CC____UUGCCC___G________ACGA____GCAAA...AUU-3'\n______________UCCG_UUAUG__UGAAG________CUG__AGAUA__GAAU______GCC_UCUGUAUC____CUAC\n______________\|\|\|\|_\|\|\|:\|__\|\|\|\|\|________\|\|\|__\|\|\|\|\|__\|\|\|:______:\|\|_\|\|\|\|\|\|:\|____\|\|\|\|\n______________AGGC_AAUGC__ACUUC________GAC__UCUAU__CUUG______UGG_AGACAUGG____GAUG\n3'-AUU...GGUUU____U____________AUUUUCCA___UU___________UAGAA_____________________-5'\n_____________\|__________________________________________________________________\|\n_____________58_________________________________________________________________1\n	255 -- 261	5 -- 11	-36.5836 kcal/mol	11.3711 kcal/mol	12.7026 kcal/mol	205 270@LB@ \| \|@LB@5'-UUG...AUGAC U AC AUUUC UU CC UUGCCC G ACGA GCAAA...AUU-3'@LB@ UCCG UUAUG UGAAG CUG AGAUA GAAU GCC UCUGUAUC CUAC@LB@ \|\|\|\| \|\|\|:\| \|\|\|\|\| \|\|\| \|\|\|\|\| \|\|\|: :\|\| \|\|\|\|\|\|:\| \|\|\|\|@LB@ AGGC AAUGC ACUUC GAC UCUAU CUUG UGG AGACAUGG GAUG@LB@3'-AUU...GGUUU U AUUUUCCA UU UAGAA -5'@LB@ \| \|@LB@ 58 1@LB@@LB@@LB@Energy -12.53 kcal/mol@LB@Hybridization Energy -36.5836 kcal/mol@LB@Unfolding Energy - mRNA 11.3711 kcal/mol@LB@Unfolding Energy - sRNA 12.7026 kcal/mol@LB@@LB@Position - mRNA 206 -- 269@LB@Position - sRNA 1 -- 57@LB@Position Seed - mRNA 255 -- 261@LB@Position Seed - sRNA 5 -- 11@LB@
138	0.004994	0.127	b3633	waaA	-9.37	0.068643	230 -- 259	48 -- 75	3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase)	_____________229____________________________260\n_____________\|______________________________\|\n5'-UCG...CUUCU___CCU__________CU_______A____GCUCU...AAC-3'\n______________CUA___UAUUCAGCCG__GAUCUGG_UACG\n______________\|\|\|___\|\|\|\|\|\|\|\|\|:__:\|\|\|:\|:_\|\|\|\|\n______________GAU___AUAAGUCGGU__UUAGGCU_AUGC\n3'-AUU...CCGCC___UUU___________________A____ACUUC...AUG-5'\n_____________\|______________________________\|\n_____________76_____________________________47\n	248 -- 254	53 -- 59	-25.3567 kcal/mol	8.33931 kcal/mol	7.67232 kcal/mol	229 260@LB@ \| \|@LB@5'-UCG...CUUCU CCU CU A GCUCU...AAC-3'@LB@ CUA UAUUCAGCCG GAUCUGG UACG@LB@ \|\|\| \|\|\|\|\|\|\|\|\|: :\|\|\|:\|: \|\|\|\|@LB@ GAU AUAAGUCGGU UUAGGCU AUGC@LB@3'-AUU...CCGCC UUU A ACUUC...AUG-5'@LB@ \| \|@LB@ 76 47@LB@@LB@@LB@Energy -9.37 kcal/mol@LB@Hybridization Energy -25.3567 kcal/mol@LB@Unfolding Energy - mRNA 8.33931 kcal/mol@LB@Unfolding Energy - sRNA 7.67232 kcal/mol@LB@@LB@Position - mRNA 230 -- 259@LB@Position - sRNA 48 -- 75@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 53 -- 59@LB@
44	0.000471	0.03766	b3624	waaZ	-7.10	0.250129	289 -- 295	1 -- 7	lipopolysaccharide KdoIII transferase; lipopolysaccharide core biosynthesis protein	_____________288_____296\n_____________\|_______\|\n5'-AGA...UUCGG_______AUCGC-3'\n______________GCCCUAC\n______________:\|\|\|\|\|\|\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	289 -- 295	1 -- 7	-11.6335 kcal/mol	2.52016 kcal/mol	2.03949 kcal/mol	288 296@LB@ \| \|@LB@5'-AGA...UUCGG AUCGC-3'@LB@ GCCCUAC@LB@ :\|\|\|\|\|\|@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -7.10 kcal/mol@LB@Hybridization Energy -11.6335 kcal/mol@LB@Unfolding Energy - mRNA 2.52016 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 289 -- 295@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 1 -- 7@LB@
116	0.003844	0.1165	b1004	wrbA	-12.42	0.007537	123 -- 145	10 -- 35	NAD(P)H:quinone oxidoreductase	_____________122________________________146\n_____________\|__________________________\|\n5'-UUA...UGCAC__________________________AAAUA...AAG-3'\n______________UCUGAAA___AGGACGUCUUAUCUUU\n______________\|\|\|\|\|\|\|___\|\|:\|\|:\|\|\|\|\|:\|\|:\|\n______________AGACUUU___UCUUGUAGAAUGGAGA\n3'-AUU...UUUCC_______CUA________________CAUGG...AUG-5'\n_____________\|__________________________\|\n_____________36_________________________9\n	133 -- 139	16 -- 22	-25.4666 kcal/mol	4.32254 kcal/mol	8.70229 kcal/mol	122 146@LB@ \| \|@LB@5'-UUA...UGCAC AAAUA...AAG-3'@LB@ UCUGAAA AGGACGUCUUAUCUUU@LB@ \|\|\|\|\|\|\| \|\|:\|\|:\|\|\|\|\|:\|\|:\|@LB@ AGACUUU UCUUGUAGAAUGGAGA@LB@3'-AUU...UUUCC CUA CAUGG...AUG-5'@LB@ \| \|@LB@ 36 9@LB@@LB@@LB@Energy -12.42 kcal/mol@LB@Hybridization Energy -25.4666 kcal/mol@LB@Unfolding Energy - mRNA 4.32254 kcal/mol@LB@Unfolding Energy - sRNA 8.70229 kcal/mol@LB@@LB@Position - mRNA 123 -- 145@LB@Position - sRNA 10 -- 35@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 16 -- 22@LB@
22	0.0001652	0.02641	b3566	xylF	-9.94	0.047349	202 -- 240	1 -- 33	D-xylose transporter subunit	_____________201_____________________________________241\n_____________\|_______________________________________\|\n5'-CGA...CACCA_____AUAA________C__CUCAC______C____C__UCCUG...AUG-3'\n______________UGAAA____AGAACAUU_UA_____CCUUUG_ACCU_AC\n______________\|\|\|\|\|____\|\|\|\|\|\|\|:_\|\|_____\|\|\|:\|\|_\|\|\|:_\|\|\n______________ACUUU____UCUUGUAG_AU_____GGAGAC_UGGG_UG\n3'-AUU...UCCAG_____CUA_________A_____________A____A__-5'\n_____________\|______________________________________\|\n_____________34_____________________________________1\n	212 -- 218	18 -- 24	-22.447 kcal/mol	3.48977 kcal/mol	9.04529 kcal/mol	201 241@LB@ \| \|@LB@5'-CGA...CACCA AUAA C CUCAC C C UCCUG...AUG-3'@LB@ UGAAA AGAACAUU UA CCUUUG ACCU AC@LB@ \|\|\|\|\| \|\|\|\|\|\|\|: \|\| \|\|\|:\|\| \|\|\|: \|\|@LB@ ACUUU UCUUGUAG AU GGAGAC UGGG UG@LB@3'-AUU...UCCAG CUA A A A -5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -9.94 kcal/mol@LB@Hybridization Energy -22.447 kcal/mol@LB@Unfolding Energy - mRNA 3.48977 kcal/mol@LB@Unfolding Energy - sRNA 9.04529 kcal/mol@LB@@LB@Position - mRNA 202 -- 240@LB@Position - sRNA 1 -- 33@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 18 -- 24@LB@
194	0.008663	0.1558	b0213	yafS	-5.39	0.521143	17 -- 34	51 -- 73	putative S-adenosyl-L-methionine-dependent methyltransferase	_____________16______________________35\n_____________\|_______________________\|\n5'-UGG...GGUGA_______________________GCCAG...UCA-3'\n______________GAAAUAU__GGCC___UCCGGUU\n______________:\|\|\|\|\|\|__:\|\|\|___\|\|\|\|:\|\|\n______________UUUUAUA__UCGG___AGGCUAA\n3'-AUU...GCCGA_______AG____UUU_______UGCAC...AUG-5'\n_____________\|_______________________\|\n_____________74______________________50\n	28 -- 34	51 -- 57	-15.2768 kcal/mol	3.69768 kcal/mol	6.213 kcal/mol	16 35@LB@ \| \|@LB@5'-UGG...GGUGA GCCAG...UCA-3'@LB@ GAAAUAU GGCC UCCGGUU@LB@ :\|\|\|\|\|\| :\|\|\| \|\|\|\|:\|\|@LB@ UUUUAUA UCGG AGGCUAA@LB@3'-AUU...GCCGA AG UUU UGCAC...AUG-5'@LB@ \| \|@LB@ 74 50@LB@@LB@@LB@Energy -5.39 kcal/mol@LB@Hybridization Energy -15.2768 kcal/mol@LB@Unfolding Energy - mRNA 3.69768 kcal/mol@LB@Unfolding Energy - sRNA 6.213 kcal/mol@LB@@LB@Position - mRNA 17 -- 34@LB@Position - sRNA 51 -- 73@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 51 -- 57@LB@
160	0.006479	0.1413	b0391	yaiE	-12.00	0.010520	148 -- 162	1 -- 15	UPF0345 family protein	_____________147_____________163\n_____________\|_______________\|\n5'-GAU...CCAGC_______GC______AGUGA...GCG-3'\n______________GCCUCUG__CCCUAU\n______________:\|\|\|\|\|\|__\|\|\|\|\|:\n______________UGGAGAC__GGGAUG\n3'-AUU...UAGAA_______AU______-5'\n_____________\|______________\|\n_____________16_____________1\n	148 -- 154	9 -- 15	-21.6181 kcal/mol	3.81195 kcal/mol	5.82808 kcal/mol	147 163@LB@ \| \|@LB@5'-GAU...CCAGC GC AGUGA...GCG-3'@LB@ GCCUCUG CCCUAU@LB@ :\|\|\|\|\|\| \|\|\|\|\|:@LB@ UGGAGAC GGGAUG@LB@3'-AUU...UAGAA AU -5'@LB@ \| \|@LB@ 16 1@LB@@LB@@LB@Energy -12.00 kcal/mol@LB@Hybridization Energy -21.6181 kcal/mol@LB@Unfolding Energy - mRNA 3.81195 kcal/mol@LB@Unfolding Energy - sRNA 5.82808 kcal/mol@LB@@LB@Position - mRNA 148 -- 162@LB@Position - sRNA 1 -- 15@LB@Position Seed - mRNA 148 -- 154@LB@Position Seed - sRNA 9 -- 15@LB@
167	0.00683	0.1439	b0387	yaiI	-9.54	0.061403	53 -- 70	51 -- 65	UPF0178 family protein	_____________52_________________71\n_____________\|__________________\|\n5'-CAC...UGCCG_______UAAAA______UUCUU...CAA-3'\n______________CAGCCAA_____CCGAUU\n______________\|\|\|\|\|\|\|_____\|\|\|\|\|\|\n______________GUCGGUU_____GGCUAA\n3'-AUU...UAUAA_______UA_________UGCAC...AUG-5'\n_____________\|__________________\|\n_____________66_________________50\n	53 -- 59	59 -- 65	-16.8495 kcal/mol	1.14267 kcal/mol	6.18987 kcal/mol	52 71@LB@ \| \|@LB@5'-CAC...UGCCG UAAAA UUCUU...CAA-3'@LB@ CAGCCAA CCGAUU@LB@ \|\|\|\|\|\|\| \|\|\|\|\|\|@LB@ GUCGGUU GGCUAA@LB@3'-AUU...UAUAA UA UGCAC...AUG-5'@LB@ \| \|@LB@ 66 50@LB@@LB@@LB@Energy -9.54 kcal/mol@LB@Hybridization Energy -16.8495 kcal/mol@LB@Unfolding Energy - mRNA 1.14267 kcal/mol@LB@Unfolding Energy - sRNA 6.18987 kcal/mol@LB@@LB@Position - mRNA 53 -- 70@LB@Position - sRNA 51 -- 65@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 59 -- 65@LB@
86	0.002239	0.09158	b0434	yajG	-10.16	0.040736	167 -- 223	4 -- 56	putative lipoprotein	_____________166____________________________________________________________224\n_____________\|______________________________________________________________\|\n5'-UGG...UUUUA__AAAC___CU_________UC________CCC______G__CAAAAAAAU_____U_____GUUAG...UGC-3'\n______________CC____ACG_____UAAGAG__UCUGAGAG___GAACAU_UU_________CCUCU___CCC\n______________\|\|____\|\|\|_____\|\|\|:\|\|__\|\|\|\|\|:\|\|___\|\|\|\|\|\|_\|\|_________\|\|\|\|\|___\|\|\|\n______________GG____UGC_____AUUUUC__AGACUUUC___CUUGUA_AA_________GGAGA___GGG\n3'-AUU...GUUUA__CUAA___ACUUC______C_________UAU______G__U_____________CAU___AUG-5'\n_____________\|______________________________________________________________\|\n_____________57_____________________________________________________________3\n	187 -- 193	28 -- 34	-29.9553 kcal/mol	7.1187 kcal/mol	12.7017 kcal/mol	166 224@LB@ \| \|@LB@5'-UGG...UUUUA AAAC CU UC CCC G CAAAAAAAU U GUUAG...UGC-3'@LB@ CC ACG UAAGAG UCUGAGAG GAACAU UU CCUCU CCC@LB@ \|\| \|\|\| \|\|\|:\|\| \|\|\|\|\|:\|\| \|\|\|\|\|\| \|\| \|\|\|\|\| \|\|\|@LB@ GG UGC AUUUUC AGACUUUC CUUGUA AA GGAGA GGG@LB@3'-AUU...GUUUA CUAA ACUUC C UAU G U CAU AUG-5'@LB@ \| \|@LB@ 57 3@LB@@LB@@LB@Energy -10.16 kcal/mol@LB@Hybridization Energy -29.9553 kcal/mol@LB@Unfolding Energy - mRNA 7.1187 kcal/mol@LB@Unfolding Energy - sRNA 12.7017 kcal/mol@LB@@LB@Position - mRNA 167 -- 223@LB@Position - sRNA 4 -- 56@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 28 -- 34@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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