Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
115	0.00375	0.1147	b1057	yceJ	-5.38	0.522888	37 -- 59	1 -- 23	putative cytochrome b561		_____________36_______________________60\n_____________\|________________________\|\n5'-UGA...CACCA_______C__AUG___A_______UAAAC...UGU-3'\n______________GACAUCU_UG___CUG_ACCC_AC\n______________:\|\|\|\|\|\|_\|:___\|\|\|_\|\|\|\|_\|\|\n______________UUGUAGA_AU___GAC_UGGG_UG\n3'-AUU...CUAUC__________GGA___A____A__-5'\n_____________\|_______________________\|\n_____________24______________________1\n	37 -- 43	17 -- 23	-17.2281 kcal/mol	3.85652 kcal/mol	8.01506 kcal/mol	36 60@LB@ \| \|@LB@5'-UGA...CACCA C AUG A UAAAC...UGU-3'@LB@ GACAUCU UG CUG ACCC AC@LB@ :\|\|\|\|\|\| \|: \|\|\| \|\|\|\| \|\|@LB@ UUGUAGA AU GAC UGGG UG@LB@3'-AUU...CUAUC GGA A A -5'@LB@ \| \|@LB@ 24 1@LB@@LB@@LB@Energy -5.38 kcal/mol@LB@Hybridization Energy -17.2281 kcal/mol@LB@Unfolding Energy - mRNA 3.85652 kcal/mol@LB@Unfolding Energy - sRNA 8.01506 kcal/mol@LB@@LB@Position - mRNA 37 -- 59@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 37 -- 43@LB@Position Seed - sRNA 17 -- 23@LB@
68	0.001174	0.05856	b1253	yciA	-8.30	0.130708	128 -- 145	3 -- 21	acyl-CoA esterase		_____________127__________________146\n_____________\|____________________\|\n5'-UUU...CGCUG______________UC____CAGGA...GGU-3'\n______________UAUCU__CCUCUGU__UCCU\n______________:\|\|\|\|__\|\|\|\|\|\|\|__:\|\|\|\n______________GUAGA__GGAGACA__GGGA\n3'-AUU...AUCUU_____AU_______U_____UG-5'\n_____________\|____________________\|\n_____________22___________________2\n	133 -- 139	8 -- 14	-17.321 kcal/mol	1.04882 kcal/mol	7.99052 kcal/mol	127 146@LB@ \| \|@LB@5'-UUU...CGCUG UC CAGGA...GGU-3'@LB@ UAUCU CCUCUGU UCCU@LB@ :\|\|\|\| \|\|\|\|\|\|\| :\|\|\|@LB@ GUAGA GGAGACA GGGA@LB@3'-AUU...AUCUU AU U UG-5'@LB@ \| \|@LB@ 22 2@LB@@LB@@LB@Energy -8.30 kcal/mol@LB@Hybridization Energy -17.321 kcal/mol@LB@Unfolding Energy - mRNA 1.04882 kcal/mol@LB@Unfolding Energy - sRNA 7.99052 kcal/mol@LB@@LB@Position - mRNA 128 -- 145@LB@Position - sRNA 3 -- 21@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 8 -- 14@LB@
5	1.055e-05	0.007422	b1287	yciW	-9.98	0.045809	266 -- 289	4 -- 27	putative oxidoreductase		_____________265______________________290\n_____________\|________________________\|\n5'-GUC...ACGGA___U______C________GU___GGAAG...CAA-3'\n______________GUA_GACGUU_UGCCUCUG__CCC\n______________:\|\|_:\|\|:\|:_\|:\|\|\|\|\|\|__\|\|\|\n______________UAU_UUGUAG_AUGGAGAC__GGG\n3'-AUU...CUUUC___C______A________AU___AUG-5'\n_____________\|________________________\|\n_____________28_______________________3\n	278 -- 284	9 -- 15	-26.8114 kcal/mol	8.80708 kcal/mol	8.0435 kcal/mol	265 290@LB@ \| \|@LB@5'-GUC...ACGGA U C GU GGAAG...CAA-3'@LB@ GUA GACGUU UGCCUCUG CCC@LB@ :\|\| :\|\|:\|: \|:\|\|\|\|\|\| \|\|\|@LB@ UAU UUGUAG AUGGAGAC GGG@LB@3'-AUU...CUUUC C A AU AUG-5'@LB@ \| \|@LB@ 28 3@LB@@LB@@LB@Energy -9.98 kcal/mol@LB@Hybridization Energy -26.8114 kcal/mol@LB@Unfolding Energy - mRNA 8.80708 kcal/mol@LB@Unfolding Energy - sRNA 8.0435 kcal/mol@LB@@LB@Position - mRNA 266 -- 289@LB@Position - sRNA 4 -- 27@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 9 -- 15@LB@
59	0.0008996	0.0531	b1310	ycjN	-10.87	0.024752	113 -- 129	42 -- 59	putative ABC sugar transporter periplasmic binding protein		_____________112________________130\n_____________\|__________________\|\n5'-UUC...GUCGG__________________UGAUA...UUA-3'\n______________GAUCUGAUUA_GUGAAGU\n______________:\|\|\|:\|\|\|\|\|_\|\|\|\|\|\|\|\n______________UUAGGCUAAU_CACUUCA\n3'-AUU...UCGGU__________G_______UUUUC...AUG-5'\n_____________\|__________________\|\n_____________60_________________41\n	123 -- 129	42 -- 48	-20.0101 kcal/mol	5.87569 kcal/mol	3.27976 kcal/mol	112 130@LB@ \| \|@LB@5'-UUC...GUCGG UGAUA...UUA-3'@LB@ GAUCUGAUUA GUGAAGU@LB@ :\|\|\|:\|\|\|\|\| \|\|\|\|\|\|\|@LB@ UUAGGCUAAU CACUUCA@LB@3'-AUU...UCGGU G UUUUC...AUG-5'@LB@ \| \|@LB@ 60 41@LB@@LB@@LB@Energy -10.87 kcal/mol@LB@Hybridization Energy -20.0101 kcal/mol@LB@Unfolding Energy - mRNA 5.87569 kcal/mol@LB@Unfolding Energy - sRNA 3.27976 kcal/mol@LB@@LB@Position - mRNA 113 -- 129@LB@Position - sRNA 42 -- 59@LB@Position Seed - mRNA 123 -- 129@LB@Position Seed - sRNA 42 -- 48@LB@
175	0.007291	0.1466	b1498	ydeN	-8.19	0.139530	95 -- 124	45 -- 67	putative Ser-type periplasmic non-aryl sulfatase	b3678 b3801	_____________94_____________________________125\n_____________\|______________________________\|\n5'-AUU...CUUUU______________CUAUCUCU________CUAAC...UAA-3'\n______________UUUAGCUAAAUCUG________UUAUGUGA\n______________\|\|:\|\|\|:\|\|\|\|\|:\|________\|\|\|:\|\|\|\|\n______________AAGUCGGUUUAGGC________AAUGCACU\n3'-AUU...UUUAU______________U_______________UCAUU...AUG-5'\n_____________\|______________________________\|\n_____________68_____________________________44\n	118 -- 124	45 -- 51	-22.2428 kcal/mol	5.90754 kcal/mol	8.16749 kcal/mol	94 125@LB@ \| \|@LB@5'-AUU...CUUUU CUAUCUCU CUAAC...UAA-3'@LB@ UUUAGCUAAAUCUG UUAUGUGA@LB@ \|\|:\|\|\|:\|\|\|\|\|:\| \|\|\|:\|\|\|\|@LB@ AAGUCGGUUUAGGC AAUGCACU@LB@3'-AUU...UUUAU U UCAUU...AUG-5'@LB@ \| \|@LB@ 68 44@LB@@LB@@LB@Energy -8.19 kcal/mol@LB@Hybridization Energy -22.2428 kcal/mol@LB@Unfolding Energy - mRNA 5.90754 kcal/mol@LB@Unfolding Energy - sRNA 8.16749 kcal/mol@LB@@LB@Position - mRNA 95 -- 124@LB@Position - sRNA 45 -- 67@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 45 -- 51@LB@
66	0.001146	0.05856	b1657	ydhP	-3.95	0.782289	131 -- 139	49 -- 57	putative MFS transporter inner membrane protein		_____________130_______140\n_____________\|_________\|\n5'-UCG...UUUUU_G_______UGUCA...GCG-3'\n______________U_UGAUUGC\n______________\|_:\|\|\|\|:\|\n______________A_GCUAAUG\n3'-AUU...GGUUU_G_______CACUU...AUG-5'\n_____________\|_________\|\n_____________58________48\n	133 -- 139	49 -- 55	-6.30818 kcal/mol	2.04371 kcal/mol	0.318327 kcal/mol	130 140@LB@ \| \|@LB@5'-UCG...UUUUU G UGUCA...GCG-3'@LB@ U UGAUUGC@LB@ \| :\|\|\|\|:\|@LB@ A GCUAAUG@LB@3'-AUU...GGUUU G CACUU...AUG-5'@LB@ \| \|@LB@ 58 48@LB@@LB@@LB@Energy -3.95 kcal/mol@LB@Hybridization Energy -6.30818 kcal/mol@LB@Unfolding Energy - mRNA 2.04371 kcal/mol@LB@Unfolding Energy - sRNA 0.318327 kcal/mol@LB@@LB@Position - mRNA 131 -- 139@LB@Position - sRNA 49 -- 57@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 49 -- 55@LB@
113	0.003703	0.1147	b1728	ydjM	-2.69	0.937889	161 -- 167	1 -- 7	inner membrane protein regulated by LexA		_____________160_____168\n_____________\|_______\|\n5'-CGC...UAAAA_______ACUGU...AUA-3'\n______________AUCCUAU\n______________\|:\|\|\|\|:\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	161 -- 167	1 -- 7	-7.15055 kcal/mol	2.44298 kcal/mol	2.03949 kcal/mol	160 168@LB@ \| \|@LB@5'-CGC...UAAAA ACUGU...AUA-3'@LB@ AUCCUAU@LB@ \|:\|\|\|\|:@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -2.69 kcal/mol@LB@Hybridization Energy -7.15055 kcal/mol@LB@Unfolding Energy - mRNA 2.44298 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 161 -- 167@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 1 -- 7@LB@
181	0.007802	0.1516	b1797	yeaR	-7.07	0.254063	142 -- 149	2 -- 9	DUF1971 family protein nitrate-inducible		_____________141______150\n_____________\|________\|\n5'-UGC...CAGGC________AAACG...AAG-3'\n______________GUACCCUG\n______________\|\|\|\|\|\|\|:\n______________CAUGGGAU\n3'-AUU...GGAGA________G-5'\n_____________\|________\|\n_____________10_______1\n	143 -- 149	2 -- 8	-12.2602 kcal/mol	3.09143 kcal/mol	2.12684 kcal/mol	141 150@LB@ \| \|@LB@5'-UGC...CAGGC AAACG...AAG-3'@LB@ GUACCCUG@LB@ \|\|\|\|\|\|\|:@LB@ CAUGGGAU@LB@3'-AUU...GGAGA G-5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -7.07 kcal/mol@LB@Hybridization Energy -12.2602 kcal/mol@LB@Unfolding Energy - mRNA 3.09143 kcal/mol@LB@Unfolding Energy - sRNA 2.12684 kcal/mol@LB@@LB@Position - mRNA 142 -- 149@LB@Position - sRNA 2 -- 9@LB@Position Seed - mRNA 143 -- 149@LB@Position Seed - sRNA 2 -- 8@LB@
9	1.988e-05	0.007771	b1985	yeeO	-11.51	0.015356	224 -- 255	1 -- 39	putative multdrug exporter MATE family		_____________223_____________________________________256\n_____________\|_______________________________________\|\n5'-GAU...CAAUU______GGCUUU___________________________GCCAG...GCU-3'\n______________AAGGUC_________GAAUAUCU__CCUCUG__CUUUAC\n______________\|\|\|\|\|\|_________\|\|\|:\|\|\|\|__\|\|\|\|\|\|__\|::\|\|\|\n______________UUCCAG_________CUUGUAGA__GGAGAC__GGGAUG\n3'-AUU...UCAUU______ACUUUCUAU________AU______AU______-5'\n_____________\|______________________________________\|\n_____________40_____________________________________1\n	237 -- 243	17 -- 23	-28.0657 kcal/mol	5.21805 kcal/mol	11.3576 kcal/mol	223 256@LB@ \| \|@LB@5'-GAU...CAAUU GGCUUU GCCAG...GCU-3'@LB@ AAGGUC GAAUAUCU CCUCUG CUUUAC@LB@ \|\|\|\|\|\| \|\|\|:\|\|\|\| \|\|\|\|\|\| \|::\|\|\|@LB@ UUCCAG CUUGUAGA GGAGAC GGGAUG@LB@3'-AUU...UCAUU ACUUUCUAU AU AU -5'@LB@ \| \|@LB@ 40 1@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -28.0657 kcal/mol@LB@Unfolding Energy - mRNA 5.21805 kcal/mol@LB@Unfolding Energy - sRNA 11.3576 kcal/mol@LB@@LB@Position - mRNA 224 -- 255@LB@Position - sRNA 1 -- 39@LB@Position Seed - mRNA 237 -- 243@LB@Position Seed - sRNA 17 -- 23@LB@
131	0.004483	0.1204	b2069	yegD	-6.32	0.360285	213 -- 222	48 -- 57	Hsp70 chaperone family protein		_____________212________223\n_____________\|__________\|\n5'-CAC...UUGGU__________GUACA...UGC-3'\n______________UUUGAUUACG\n______________\|::\|\|\|\|\|\|\|\n______________AGGCUAAUGC\n3'-AUU...GGUUU__________ACUUC...AUG-5'\n_____________\|__________\|\n_____________58_________47\n	216 -- 222	48 -- 54	-10.2333 kcal/mol	2.33693 kcal/mol	1.55976 kcal/mol	212 223@LB@ \| \|@LB@5'-CAC...UUGGU GUACA...UGC-3'@LB@ UUUGAUUACG@LB@ \|::\|\|\|\|\|\|\|@LB@ AGGCUAAUGC@LB@3'-AUU...GGUUU ACUUC...AUG-5'@LB@ \| \|@LB@ 58 47@LB@@LB@@LB@Energy -6.32 kcal/mol@LB@Hybridization Energy -10.2333 kcal/mol@LB@Unfolding Energy - mRNA 2.33693 kcal/mol@LB@Unfolding Energy - sRNA 1.55976 kcal/mol@LB@@LB@Position - mRNA 213 -- 222@LB@Position - sRNA 48 -- 57@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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