Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
111	0.01894	0.5385	b2600	tyrA	-7.88	0.238290	191 -- 206	74 -- 89	fused chorismate mutase T/prephenate dehydrogenase	_____________190______________207\n_____________\|________________\|\n5'-UCG...CUUAA_________UA_____GCUGA...UGG-3'\n______________GAGGUUUAU__UGGUU\n______________:\|:\|\|\|:\|:__\|\|\|:\|\n______________UUUCAAGUG__ACCGA\n3'-CGC...GGCGU_________CA_____AAUCA...AAG-5'\n_____________\|________________\|\n_____________90_______________73\n	193 -- 199	81 -- 87	-12.4695 kcal/mol	4.06959 kcal/mol	0.517373 kcal/mol	190 207@LB@ \| \|@LB@5'-UCG...CUUAA UA GCUGA...UGG-3'@LB@ GAGGUUUAU UGGUU@LB@ :\|:\|\|\|:\|: \|\|\|:\|@LB@ UUUCAAGUG ACCGA@LB@3'-CGC...GGCGU CA AAUCA...AAG-5'@LB@ \| \|@LB@ 90 73@LB@@LB@@LB@Energy -7.88 kcal/mol@LB@Hybridization Energy -12.4695 kcal/mol@LB@Unfolding Energy - mRNA 4.06959 kcal/mol@LB@Unfolding Energy - sRNA 0.517373 kcal/mol@LB@@LB@Position - mRNA 191 -- 206@LB@Position - sRNA 74 -- 89@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 81 -- 87@LB@
58	0.01138	0.5385	b2595	bamD	-7.85	0.240744	21 -- 29	72 -- 80	BamABCDE complex OM biogenesis lipoprotein	_____________20________30\n_____________\|_________\|\n5'-CUG...ACCGA_________CGGAC...AUG-3'\n______________GUUGGUUUU\n______________\|\|\|\|\|:\|\|\|\n______________CAACCGAAA\n3'-CGC...AAGUG_________UCAAU...AAG-5'\n_____________\|_________\|\n_____________81________71\n	23 -- 29	72 -- 78	-10.2023 kcal/mol	2.1418 kcal/mol	0.205868 kcal/mol	20 30@LB@ \| \|@LB@5'-CUG...ACCGA CGGAC...AUG-3'@LB@ GUUGGUUUU@LB@ \|\|\|\|\|:\|\|\|@LB@ CAACCGAAA@LB@3'-CGC...AAGUG UCAAU...AAG-5'@LB@ \| \|@LB@ 81 71@LB@@LB@@LB@Energy -7.85 kcal/mol@LB@Hybridization Energy -10.2023 kcal/mol@LB@Unfolding Energy - mRNA 2.1418 kcal/mol@LB@Unfolding Energy - sRNA 0.205868 kcal/mol@LB@@LB@Position - mRNA 21 -- 29@LB@Position - sRNA 72 -- 80@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 72 -- 78@LB@
176	0.03112	0.5559	b2536	hcaT	-7.63	0.261550	240 -- 259	67 -- 85	putative 3-phenylpropionic transporter	_____________239__________________260\n_____________\|____________________\|\n5'-UCG...GCUAU_____AUACUU_________CGGCA...GGA-3'\n______________UUCAC______UUUUAGUUA\n______________\|\|\|\|\|______:\|\|\|\|\|\|\|\|\n______________AAGUG______GAAAUCAAU\n3'-CGC...UUUUC_____CAACC__________AAGCU...AAG-5'\n_____________\|____________________\|\n_____________86___________________66\n	253 -- 259	67 -- 73	-10.0019 kcal/mol	1.80629 kcal/mol	0.569003 kcal/mol	239 260@LB@ \| \|@LB@5'-UCG...GCUAU AUACUU CGGCA...GGA-3'@LB@ UUCAC UUUUAGUUA@LB@ \|\|\|\|\| :\|\|\|\|\|\|\|\|@LB@ AAGUG GAAAUCAAU@LB@3'-CGC...UUUUC CAACC AAGCU...AAG-5'@LB@ \| \|@LB@ 86 66@LB@@LB@@LB@Energy -7.63 kcal/mol@LB@Hybridization Energy -10.0019 kcal/mol@LB@Unfolding Energy - mRNA 1.80629 kcal/mol@LB@Unfolding Energy - sRNA 0.569003 kcal/mol@LB@@LB@Position - mRNA 240 -- 259@LB@Position - sRNA 67 -- 85@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 67 -- 73@LB@
164	0.02889	0.5559	b1512	lsrR	-7.59	0.264791	265 -- 277	71 -- 84	lsr operon transcriptional repressor	_____________264____________278\n_____________\|______________\|\n5'-GCU...GCGGA______________CUAUC...AGA-3'\n______________UCGCG_UGGUUUUA\n______________\|\|:\|\|_\|\|\|:\|\|\|\|\n______________AGUGC_ACCGAAAU\n3'-CGC...UUUCA_____A________CAAUA...AAG-5'\n_____________\|______________\|\n_____________85_____________70\n	271 -- 277	71 -- 77	-14.8882 kcal/mol	6.73904 kcal/mol	0.5567 kcal/mol	264 278@LB@ \| \|@LB@5'-GCU...GCGGA CUAUC...AGA-3'@LB@ UCGCG UGGUUUUA@LB@ \|\|:\|\| \|\|\|:\|\|\|\|@LB@ AGUGC ACCGAAAU@LB@3'-CGC...UUUCA A CAAUA...AAG-5'@LB@ \| \|@LB@ 85 70@LB@@LB@@LB@Energy -7.59 kcal/mol@LB@Hybridization Energy -14.8882 kcal/mol@LB@Unfolding Energy - mRNA 6.73904 kcal/mol@LB@Unfolding Energy - sRNA 0.5567 kcal/mol@LB@@LB@Position - mRNA 265 -- 277@LB@Position - sRNA 71 -- 84@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 71 -- 77@LB@
127	0.02181	0.5385	b3057	bacA	-7.57	0.267037	62 -- 69	75 -- 82	undecaprenyl pyrophosphate phosphatase	_____________61_______70\n_____________\|________\|\n5'-UUC...GGCGA________GUAAU...UUG-3'\n______________AUGUUGGC\n______________\|:\|\|\|\|\|\|\n______________UGCAACCG\n3'-CGC...UCAAG________AAAUC...AAG-5'\n_____________\|________\|\n_____________83_______74\n	63 -- 69	75 -- 81	-12.0605 kcal/mol	4.15225 kcal/mol	0.339284 kcal/mol	61 70@LB@ \| \|@LB@5'-UUC...GGCGA GUAAU...UUG-3'@LB@ AUGUUGGC@LB@ \|:\|\|\|\|\|\|@LB@ UGCAACCG@LB@3'-CGC...UCAAG AAAUC...AAG-5'@LB@ \| \|@LB@ 83 74@LB@@LB@@LB@Energy -7.57 kcal/mol@LB@Hybridization Energy -12.0605 kcal/mol@LB@Unfolding Energy - mRNA 4.15225 kcal/mol@LB@Unfolding Energy - sRNA 0.339284 kcal/mol@LB@@LB@Position - mRNA 62 -- 69@LB@Position - sRNA 75 -- 82@LB@Position Seed - mRNA 63 -- 69@LB@Position Seed - sRNA 75 -- 81@LB@
186	0.03285	0.5559	b1003	yccJ	-7.53	0.270746	41 -- 54	75 -- 88	uncharacterized protein	_____________40_____________55\n_____________\|______________\|\n5'-CCU...UUCAC_________GC___ACGCC...GGU-3'\n______________AGGUUCGCG__GGU\n______________\|:\|\|\|\|:\|\|__\|\|:\n______________UUCAAGUGC__CCG\n3'-CGC...GCGUU_________AA___AAAUC...AAG-5'\n_____________\|______________\|\n_____________89_____________74\n	43 -- 49	80 -- 86	-16.6206 kcal/mol	8.57413 kcal/mol	0.515884 kcal/mol	40 55@LB@ \| \|@LB@5'-CCU...UUCAC GC ACGCC...GGU-3'@LB@ AGGUUCGCG GGU@LB@ \|:\|\|\|\|:\|\| \|\|:@LB@ UUCAAGUGC CCG@LB@3'-CGC...GCGUU AA AAAUC...AAG-5'@LB@ \| \|@LB@ 89 74@LB@@LB@@LB@Energy -7.53 kcal/mol@LB@Hybridization Energy -16.6206 kcal/mol@LB@Unfolding Energy - mRNA 8.57413 kcal/mol@LB@Unfolding Energy - sRNA 0.515884 kcal/mol@LB@@LB@Position - mRNA 41 -- 54@LB@Position - sRNA 75 -- 88@LB@Position Seed - mRNA 43 -- 49@LB@Position Seed - sRNA 80 -- 86@LB@
196	0.0344	0.5559	b0954	fabA	-7.50	0.274211	100 -- 110	74 -- 84	beta-hydroxydecanoyl thioester dehydrase	_____________99__________111\n_____________\|___________\|\n5'-GUG...GGCCA___________GAACU...ACA-3'\n______________UUACGUUGGCU\n______________\|:\|\|\|\|\|\|\|\|\|\n______________AGUGCAACCGA\n3'-CGC...UUUCA___________AAUCA...AAG-5'\n_____________\|___________\|\n_____________85__________73\n	104 -- 110	74 -- 80	-16.8122 kcal/mol	8.80896 kcal/mol	0.508159 kcal/mol	99 111@LB@ \| \|@LB@5'-GUG...GGCCA GAACU...ACA-3'@LB@ UUACGUUGGCU@LB@ \|:\|\|\|\|\|\|\|\|\|@LB@ AGUGCAACCGA@LB@3'-CGC...UUUCA AAUCA...AAG-5'@LB@ \| \|@LB@ 85 73@LB@@LB@@LB@Energy -7.50 kcal/mol@LB@Hybridization Energy -16.8122 kcal/mol@LB@Unfolding Energy - mRNA 8.80896 kcal/mol@LB@Unfolding Energy - sRNA 0.508159 kcal/mol@LB@@LB@Position - mRNA 100 -- 110@LB@Position - sRNA 74 -- 84@LB@Position Seed - mRNA 104 -- 110@LB@Position Seed - sRNA 74 -- 80@LB@
140	0.02329	0.5385	b3026	qseC	-7.47	0.277092	174 -- 202	52 -- 78	quorum sensing sensory histidine kinase in two-component regulatory system with QseB	_____________173___________________________203\n_____________\|_____________________________\|\n5'-AAA...GUGCA____A_______CA_AUU__G________GAAAU...GGA-3'\n______________UGGU_UUGGUUA__C___AG_UGAGAAAU\n______________\|\|\|:_\|\|:\|\|\|\|__\|___\|\|_\|\|\|\|\|\|\|:\n______________ACCG_AAUCAAU__G___UC_ACUCUUUG\n3'-CGC...GUGCA____A_______AA_C____A________AGAGC...AAG-5'\n_____________\|_____________________________\|\n_____________79____________________________51\n	196 -- 202	52 -- 58	-18.6962 kcal/mol	6.8409 kcal/mol	4.38944 kcal/mol	173 203@LB@ \| \|@LB@5'-AAA...GUGCA A CA AUU G GAAAU...GGA-3'@LB@ UGGU UUGGUUA C AG UGAGAAAU@LB@ \|\|\|: \|\|:\|\|\|\| \| \|\| \|\|\|\|\|\|\|:@LB@ ACCG AAUCAAU G UC ACUCUUUG@LB@3'-CGC...GUGCA A AA C A AGAGC...AAG-5'@LB@ \| \|@LB@ 79 51@LB@@LB@@LB@Energy -7.47 kcal/mol@LB@Hybridization Energy -18.6962 kcal/mol@LB@Unfolding Energy - mRNA 6.8409 kcal/mol@LB@Unfolding Energy - sRNA 4.38944 kcal/mol@LB@@LB@Position - mRNA 174 -- 202@LB@Position - sRNA 52 -- 78@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 52 -- 58@LB@
191	0.03353	0.5559	b2129	yehX	-7.36	0.287226	59 -- 67	69 -- 77	putative ABC transporter ATPase	_____________58________68\n_____________\|_________\|\n5'-GGU...CAGGC_________GCGCU...UUG-3'\n______________GGUUUUGGU\n______________\|\|:\|\|\|:\|\|\n______________CCGAAAUCA\n3'-CGC...UGCAA_________AUAAG...AAG-5'\n_____________\|_________\|\n_____________78________68\n	61 -- 67	69 -- 75	-10.3561 kcal/mol	2.74802 kcal/mol	0.243235 kcal/mol	58 68@LB@ \| \|@LB@5'-GGU...CAGGC GCGCU...UUG-3'@LB@ GGUUUUGGU@LB@ \|\|:\|\|\|:\|\|@LB@ CCGAAAUCA@LB@3'-CGC...UGCAA AUAAG...AAG-5'@LB@ \| \|@LB@ 78 68@LB@@LB@@LB@Energy -7.36 kcal/mol@LB@Hybridization Energy -10.3561 kcal/mol@LB@Unfolding Energy - mRNA 2.74802 kcal/mol@LB@Unfolding Energy - sRNA 0.243235 kcal/mol@LB@@LB@Position - mRNA 59 -- 67@LB@Position - sRNA 69 -- 77@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 69 -- 75@LB@
141	0.02331	0.5385	b1844	exoX	-7.22	0.302733	200 -- 217	62 -- 83	exodeoxyribonuclease 10; DNA exonuclease X	_____________199_____________________218\n_____________\|_______________________\|\n5'-UUA...CAUGC_______C__A____________UACAG...UGA-3'\n______________CAUGUUG_GC_UUA_____UCGA\n______________\|\|:\|\|\|\|_\|\|_\|\|\|_____\|\|\|\|\n______________GUGCAAC_CG_AAU_____AGCU\n3'-CGC...UUCAA__________A___CAAUA____CAACU...AAG-5'\n_____________\|_______________________\|\n_____________84______________________61\n	200 -- 206	77 -- 83	-13.618 kcal/mol	4.77882 kcal/mol	1.62352 kcal/mol	199 218@LB@ \| \|@LB@5'-UUA...CAUGC C A UACAG...UGA-3'@LB@ CAUGUUG GC UUA UCGA@LB@ \|\|:\|\|\|\| \|\| \|\|\| \|\|\|\|@LB@ GUGCAAC CG AAU AGCU@LB@3'-CGC...UUCAA A CAAUA CAACU...AAG-5'@LB@ \| \|@LB@ 84 61@LB@@LB@@LB@Energy -7.22 kcal/mol@LB@Hybridization Energy -13.618 kcal/mol@LB@Unfolding Energy - mRNA 4.77882 kcal/mol@LB@Unfolding Energy - sRNA 1.62352 kcal/mol@LB@@LB@Position - mRNA 200 -- 217@LB@Position - sRNA 62 -- 83@LB@Position Seed - mRNA 200 -- 206@LB@Position Seed - sRNA 77 -- 83@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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