Input and runtime details for job 6038639 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6038639 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:35:57 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:19:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:35:30 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:35:37 CET 2018
	Job Completed		@ Fri Feb 16 21:35:51 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6038639&toolName=CopraRNA ( 30 days expiry )

Description of the job

IhtA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000117 Chlamydia trachomatis D/UW-3/CX

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
171	0.07267	0.3448	ct_057	gcpE	-5.03	0.358241	27 -- 78	18 -- 60	4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)	_____________26____________________________________________________79\n_____________\|_____________________________________________________\|\n5'-AGA...GAACA____UCAA_______AAUU________AUUUUUAAAG_______UC_______AGACC...AAU-3'\n______________UCCC____CCC_GUG____UAUAAACA__________GGAAGUU__UCCAUGG\n______________\|\|\|\|____\|\|\|_\|\|\|____\|\|:\|\|\|\|\|__________:\|\|\|\|:\|__\|\|\|\|\|\|\|\n______________AGGG____GGG_CAC____AUGUUUGU__________UCUUCGA__AGGUACC\n3'-UUU...ACUGA___________A_______________GAUCUCAG_________UA_______GCAAU...GAA-5'\n_____________\|_____________________________________________________\|\n_____________61____________________________________________________17\n	72 -- 78	18 -- 24	-36.1086 kcal/mol	16.1769 kcal/mol	14.9054 kcal/mol	26 79@LB@ \| \|@LB@5'-AGA...GAACA UCAA AAUU AUUUUUAAAG UC AGACC...AAU-3'@LB@ UCCC CCC GUG UAUAAACA GGAAGUU UCCAUGG@LB@ \|\|\|\| \|\|\| \|\|\| \|\|:\|\|\|\|\| :\|\|\|\|:\| \|\|\|\|\|\|\|@LB@ AGGG GGG CAC AUGUUUGU UCUUCGA AGGUACC@LB@3'-UUU...ACUGA A GAUCUCAG UA GCAAU...GAA-5'@LB@ \| \|@LB@ 61 17@LB@@LB@@LB@Energy -5.03 kcal/mol@LB@Hybridization Energy -36.1086 kcal/mol@LB@Unfolding Energy - mRNA 16.1769 kcal/mol@LB@Unfolding Energy - sRNA 14.9054 kcal/mol@LB@@LB@Position - mRNA 27 -- 78@LB@Position - sRNA 18 -- 60@LB@Position Seed - mRNA 72 -- 78@LB@Position Seed - sRNA 18 -- 24@LB@
172	0.07285	0.3448	ct_090	lcrD	-6.84	0.155915	81 -- 102	18 -- 39	low calcium response protein D	_____________80______________________103\n_____________\|_______________________\|\n5'-AUU...GGAAA___________U________GA_ACCAC...UGU-3'\n______________AGAGUU_GAAG_UUAUUCCA__G\n______________\|\|\|\|\|:_\|\|\|\|_:\|\|\|\|\|\|\|__\|\n______________UCUCAG_CUUC_GAUAAGGU__C\n3'-UUU...UGUGA______U_____________AC_GCAAU...GAA-5'\n_____________\|_______________________\|\n_____________40______________________17\n	93 -- 99	21 -- 27	-20.344 kcal/mol	3.91171 kcal/mol	9.59078 kcal/mol	80 103@LB@ \| \|@LB@5'-AUU...GGAAA U GA ACCAC...UGU-3'@LB@ AGAGUU GAAG UUAUUCCA G@LB@ \|\|\|\|\|: \|\|\|\| :\|\|\|\|\|\|\| \|@LB@ UCUCAG CUUC GAUAAGGU C@LB@3'-UUU...UGUGA U AC GCAAU...GAA-5'@LB@ \| \|@LB@ 40 17@LB@@LB@@LB@Energy -6.84 kcal/mol@LB@Hybridization Energy -20.344 kcal/mol@LB@Unfolding Energy - mRNA 3.91171 kcal/mol@LB@Unfolding Energy - sRNA 9.59078 kcal/mol@LB@@LB@Position - mRNA 81 -- 102@LB@Position - sRNA 18 -- 39@LB@Position Seed - mRNA 93 -- 99@LB@Position Seed - sRNA 21 -- 27@LB@
173	0.07335	0.3448	ct_543	hisS	-6.45	0.189015	1 -- 7	82 -- 88	histidine--tRNA ligase	______________1______8\n______________\|______\|\n___________5'-_______GUAUU...AGC-3'\n______________AUGCGGC\n______________\|\|:\|\|\|\|\n______________UAUGCCG\n3'-UUU...GCCUC_______AUUGA...GAA-5'\n_____________\|_______\|\n_____________89______81\n	1 -- 7	82 -- 88	-9.9342 kcal/mol	2.38776 kcal/mol	1.09753 kcal/mol	1 8@LB@ \| \|@LB@ 5'- GUAUU...AGC-3'@LB@ AUGCGGC@LB@ \|\|:\|\|\|\|@LB@ UAUGCCG@LB@3'-UUU...GCCUC AUUGA...GAA-5'@LB@ \| \|@LB@ 89 81@LB@@LB@@LB@Energy -6.45 kcal/mol@LB@Hybridization Energy -9.9342 kcal/mol@LB@Unfolding Energy - mRNA 2.38776 kcal/mol@LB@Unfolding Energy - sRNA 1.09753 kcal/mol@LB@@LB@Position - mRNA 1 -- 7@LB@Position - sRNA 82 -- 88@LB@Position Seed - mRNA 1 -- 7@LB@Position Seed - sRNA 82 -- 88@LB@
174	0.07335	0.3448	ct_777	bioF	-7.25	0.126962	76 -- 92	19 -- 34	8-amino-7-oxononanoate synthase	_____________75________________93\n_____________\|_________________\|\n5'-CUG...UCAGG_______G_____U___UUGGG...UUU-3'\n______________UAGAAGC_AUUCC_AUG\n______________:\|\|\|\|\|\|_\|\|\|\|\|_\|\|\|\n______________GUCUUCG_UAAGG_UAC\n3'-UUU...UCUCA_______A_________CGCAA...GAA-5'\n_____________\|_________________\|\n_____________35________________18\n	76 -- 82	28 -- 34	-16.4612 kcal/mol	4.23516 kcal/mol	4.97732 kcal/mol	75 93@LB@ \| \|@LB@5'-CUG...UCAGG G U UUGGG...UUU-3'@LB@ UAGAAGC AUUCC AUG@LB@ :\|\|\|\|\|\| \|\|\|\|\| \|\|\|@LB@ GUCUUCG UAAGG UAC@LB@3'-UUU...UCUCA A CGCAA...GAA-5'@LB@ \| \|@LB@ 35 18@LB@@LB@@LB@Energy -7.25 kcal/mol@LB@Hybridization Energy -16.4612 kcal/mol@LB@Unfolding Energy - mRNA 4.23516 kcal/mol@LB@Unfolding Energy - sRNA 4.97732 kcal/mol@LB@@LB@Position - mRNA 76 -- 92@LB@Position - sRNA 19 -- 34@LB@Position Seed - mRNA 76 -- 82@LB@Position Seed - sRNA 28 -- 34@LB@
175	0.07342	0.3448	ct_738	yycJ	-7.78	0.096435	160 -- 204	18 -- 50	Zn-dependent hydrolase	_____________159____________________________________________205\n_____________\|______________________________________________\|\n5'-CAU...ACCGC_____________UCCAAUUAUCAAA______C_A___A_______AAGGU...AAC-3'\n______________GU_CAAAUACUAG_____________UCAGGA_G_UUA_UCCAUGG\n______________\|\|_\|\|\|\|:\|\|\|\|\|_____________\|\|\|\|:\|_\|_:\|\|_\|\|\|\|\|\|\|\n______________CA_GUUUGUGAUC_____________AGUCUU_C_GAU_AGGUACC\n3'-UUU...GGACA__U__________UC_______________________A_______GCAAU...GAA-5'\n_____________\|______________________________________________\|\n_____________51_____________________________________________17\n	198 -- 204	18 -- 24	-28.1471 kcal/mol	7.4527 kcal/mol	12.9164 kcal/mol	159 205@LB@ \| \|@LB@5'-CAU...ACCGC UCCAAUUAUCAAA C A A AAGGU...AAC-3'@LB@ GU CAAAUACUAG UCAGGA G UUA UCCAUGG@LB@ \|\| \|\|\|\|:\|\|\|\|\| \|\|\|\|:\| \| :\|\| \|\|\|\|\|\|\|@LB@ CA GUUUGUGAUC AGUCUU C GAU AGGUACC@LB@3'-UUU...GGACA U UC A GCAAU...GAA-5'@LB@ \| \|@LB@ 51 17@LB@@LB@@LB@Energy -7.78 kcal/mol@LB@Hybridization Energy -28.1471 kcal/mol@LB@Unfolding Energy - mRNA 7.4527 kcal/mol@LB@Unfolding Energy - sRNA 12.9164 kcal/mol@LB@@LB@Position - mRNA 160 -- 204@LB@Position - sRNA 18 -- 50@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 18 -- 24@LB@
176	0.07518	0.3511	ct_481	N/A	-6.18	0.214715	76 -- 94	18 -- 35		_____________75__________________95\n_____________\|___________________\|\n5'-AUA...CCAUU__U___AU___________AAACA...AUU-3'\n______________UC_GAA__CUGUUCUAUGG\n______________\|\|_\|\|\|__\|\|:\|\|\|:\|\|\|\|\n______________AG_CUU__GAUAAGGUACC\n3'-UUU...AUCUC__U___C____________GCAAU...GAA-5'\n_____________\|___________________\|\n_____________36__________________17\n	88 -- 94	18 -- 24	-16.4052 kcal/mol	5.24588 kcal/mol	4.97803 kcal/mol	75 95@LB@ \| \|@LB@5'-AUA...CCAUU U AU AAACA...AUU-3'@LB@ UC GAA CUGUUCUAUGG@LB@ \|\| \|\|\| \|\|:\|\|\|:\|\|\|\|@LB@ AG CUU GAUAAGGUACC@LB@3'-UUU...AUCUC U C GCAAU...GAA-5'@LB@ \| \|@LB@ 36 17@LB@@LB@@LB@Energy -6.18 kcal/mol@LB@Hybridization Energy -16.4052 kcal/mol@LB@Unfolding Energy - mRNA 5.24588 kcal/mol@LB@Unfolding Energy - sRNA 4.97803 kcal/mol@LB@@LB@Position - mRNA 76 -- 94@LB@Position - sRNA 18 -- 35@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 18 -- 24@LB@
177	0.07605	0.3532	ct_428	ubiE	-3.67	0.587989	9 -- 17	20 -- 28	demethylmenaquinone methyltransferase	_____________8_________18\n_____________\|_________\|\n___5'-UUCUUCAA_________UUCAG...AUA-3'\n______________CUAUUUCAU\n______________\|\|\|\|\|:\|\|\|\n______________GAUAAGGUA\n3'-UUU...UCUUC_________CCGCA...GAA-5'\n_____________\|_________\|\n_____________29________19\n	10 -- 16	21 -- 27	-8.26525 kcal/mol	0.050804 kcal/mol	4.54807 kcal/mol	8 18@LB@ \| \|@LB@ 5'-UUCUUCAA UUCAG...AUA-3'@LB@ CUAUUUCAU@LB@ \|\|\|\|\|:\|\|\|@LB@ GAUAAGGUA@LB@3'-UUU...UCUUC CCGCA...GAA-5'@LB@ \| \|@LB@ 29 19@LB@@LB@@LB@Energy -3.67 kcal/mol@LB@Hybridization Energy -8.26525 kcal/mol@LB@Unfolding Energy - mRNA 0.050804 kcal/mol@LB@Unfolding Energy - sRNA 4.54807 kcal/mol@LB@@LB@Position - mRNA 9 -- 17@LB@Position - sRNA 20 -- 28@LB@Position Seed - mRNA 10 -- 16@LB@Position Seed - sRNA 21 -- 27@LB@
178	0.07667	0.3541	ct_386	N/A	-3.82	0.559485	134 -- 140	82 -- 88		_____________133_____141\n_____________\|_______\|\n5'-CUU...UUUGG_______ACUAC...AGA-3'\n______________AUAUGGU\n______________\|\|\|:\|\|:\n______________UAUGCCG\n3'-UUU...GCCUC_______AUUGA...GAA-5'\n_____________\|_______\|\n_____________89______81\n	134 -- 140	82 -- 88	-6.88346 kcal/mol	1.9622 kcal/mol	1.09753 kcal/mol	133 141@LB@ \| \|@LB@5'-CUU...UUUGG ACUAC...AGA-3'@LB@ AUAUGGU@LB@ \|\|\|:\|\|:@LB@ UAUGCCG@LB@3'-UUU...GCCUC AUUGA...GAA-5'@LB@ \| \|@LB@ 89 81@LB@@LB@@LB@Energy -3.82 kcal/mol@LB@Hybridization Energy -6.88346 kcal/mol@LB@Unfolding Energy - mRNA 1.9622 kcal/mol@LB@Unfolding Energy - sRNA 1.09753 kcal/mol@LB@@LB@Position - mRNA 134 -- 140@LB@Position - sRNA 82 -- 88@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 82 -- 88@LB@
179	0.07718	0.3544	ct_781	lysS	-5.99	0.234962	194 -- 201	79 -- 86	lysine--tRNA ligase	_____________193______202\n_____________\|________\|\n5'-AAA...AAAGG________UGUCU...AUC-3'\n______________ACGGUUGA\n______________\|\|\|\|:\|:\|\n______________UGCCGAUU\n3'-UUU...CUCUA________GAGGU...GAA-5'\n_____________\|________\|\n_____________87_______78\n	195 -- 201	79 -- 85	-9.06205 kcal/mol	1.97593 kcal/mol	1.09752 kcal/mol	193 202@LB@ \| \|@LB@5'-AAA...AAAGG UGUCU...AUC-3'@LB@ ACGGUUGA@LB@ \|\|\|\|:\|:\|@LB@ UGCCGAUU@LB@3'-UUU...CUCUA GAGGU...GAA-5'@LB@ \| \|@LB@ 87 78@LB@@LB@@LB@Energy -5.99 kcal/mol@LB@Hybridization Energy -9.06205 kcal/mol@LB@Unfolding Energy - mRNA 1.97593 kcal/mol@LB@Unfolding Energy - sRNA 1.09752 kcal/mol@LB@@LB@Position - mRNA 194 -- 201@LB@Position - sRNA 79 -- 86@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 79 -- 85@LB@
180	0.07821	0.3571	ct_831	murB	-7.63	0.104376	230 -- 248	17 -- 33	UDP-N-acetylenolpyruvoylglucosamine reductase	_____________229_________________249\n_____________\|___________________\|\n5'-GUU...GUUCA____U_________UAA__CGCUU...UAA-3'\n______________AGAA_CUGUUCCAU___GC\n______________\|\|\|\|_\|\|:\|\|\|\|\|\|___\|\|\n______________UCUU_GAUAAGGUA___CG\n3'-UUU...CUCAG____C_________C____CAAUC...GAA-5'\n_____________\|___________________\|\n_____________34__________________16\n	237 -- 243	20 -- 26	-14.8604 kcal/mol	2.25495 kcal/mol	4.97804 kcal/mol	229 249@LB@ \| \|@LB@5'-GUU...GUUCA U UAA CGCUU...UAA-3'@LB@ AGAA CUGUUCCAU GC@LB@ \|\|\|\| \|\|:\|\|\|\|\|\| \|\|@LB@ UCUU GAUAAGGUA CG@LB@3'-UUU...CUCAG C C CAAUC...GAA-5'@LB@ \| \|@LB@ 34 16@LB@@LB@@LB@Energy -7.63 kcal/mol@LB@Hybridization Energy -14.8604 kcal/mol@LB@Unfolding Energy - mRNA 2.25495 kcal/mol@LB@Unfolding Energy - sRNA 4.97804 kcal/mol@LB@@LB@Position - mRNA 230 -- 248@LB@Position - sRNA 17 -- 33@LB@Position Seed - mRNA 237 -- 243@LB@Position Seed - sRNA 20 -- 26@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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