Input and runtime details for job 6038639 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6038639 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:35:57 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:19:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:35:30 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:35:37 CET 2018
	Job Completed		@ Fri Feb 16 21:35:51 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6038639&toolName=CopraRNA ( 30 days expiry )

Description of the job

IhtA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000117 Chlamydia trachomatis D/UW-3/CX

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
91	0.03287	0.2942	ct_674	yscC	-7.61	0.105180	32 -- 46	36 -- 57	type II secretion system protein	_____________31_____________________47\n_____________\|______________________\|\n5'-UGG...ACACA______________G_______AUUUG...CGG-3'\n______________CCCC_______AAA_GCUAGAG\n______________\|\|\|\|_______\|\|\|_:\|\|\|\|\|\|\n______________GGGG_______UUU_UGAUCUC\n3'-UUU...GAAGG____ACACAUG___G_______AGUCU...GAA-5'\n_____________\|______________________\|\n_____________58_____________________35\n	40 -- 46	36 -- 42	-18.3 kcal/mol	2.71277 kcal/mol	7.97448 kcal/mol	31 47@LB@ \| \|@LB@5'-UGG...ACACA G AUUUG...CGG-3'@LB@ CCCC AAA GCUAGAG@LB@ \|\|\|\| \|\|\| :\|\|\|\|\|\|@LB@ GGGG UUU UGAUCUC@LB@3'-UUU...GAAGG ACACAUG G AGUCU...GAA-5'@LB@ \| \|@LB@ 58 35@LB@@LB@@LB@Energy -7.61 kcal/mol@LB@Hybridization Energy -18.3 kcal/mol@LB@Unfolding Energy - mRNA 2.71277 kcal/mol@LB@Unfolding Energy - sRNA 7.97448 kcal/mol@LB@@LB@Position - mRNA 32 -- 46@LB@Position - sRNA 36 -- 57@LB@Position Seed - mRNA 40 -- 46@LB@Position Seed - sRNA 36 -- 42@LB@
92	0.03292	0.2942	ct_558	lipA	-15.60	0.000793	187 -- 244	3 -- 58	lipoyl synthase	_____________186_________________________________________________________245\n_____________\|___________________________________________________________\|\n5'-ACU...AUAUG_______CUAAA_____ACC__U______UCUC_______UAAAAAAUCU______C__CAGAU...AAA-3'\n______________CCCCCUG_____AAAUG___GA_UCAGAA____CUAUUCC___________AUACC_GC\n______________\|\|\|\|\|\|\|_____\|\|\|::___\|\|_\|\|\|\|\|\|____\|\|\|\|\|\|\|___________\|\|\|\|\|_:\|\n______________GGGGGAC_____UUUGU___CU_AGUCUU____GAUAAGG___________UAUGG_UG\n3'-UUU...UGAAG_______ACAUG_____GAU__C______C__________UACCGCAAUCU_____U__AA-5'\n_____________\|___________________________________________________________\|\n_____________59__________________________________________________________2\n	220 -- 226	22 -- 28	-35.9268 kcal/mol	3.77883 kcal/mol	16.5523 kcal/mol	186 245@LB@ \| \|@LB@5'-ACU...AUAUG CUAAA ACC U UCUC UAAAAAAUCU C CAGAU...AAA-3'@LB@ CCCCCUG AAAUG GA UCAGAA CUAUUCC AUACC GC@LB@ \|\|\|\|\|\|\| \|\|\|:: \|\| \|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| :\|@LB@ GGGGGAC UUUGU CU AGUCUU GAUAAGG UAUGG UG@LB@3'-UUU...UGAAG ACAUG GAU C C UACCGCAAUCU U AA-5'@LB@ \| \|@LB@ 59 2@LB@@LB@@LB@Energy -15.60 kcal/mol@LB@Hybridization Energy -35.9268 kcal/mol@LB@Unfolding Energy - mRNA 3.77883 kcal/mol@LB@Unfolding Energy - sRNA 16.5523 kcal/mol@LB@@LB@Position - mRNA 187 -- 244@LB@Position - sRNA 3 -- 58@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 22 -- 28@LB@
93	0.03342	0.2954	ct_815	mrsA_2	-4.95	0.370159	203 -- 227	23 -- 45	phosphoglucosamine mutase	_____________202_______________________228\n_____________\|_________________________\|\n5'-UAC...GCAAU_____GU__U__G___C________GGAAC...AGG-3'\n______________GACAC__GA_GU_AGA_AGCUAUUC\n______________:\|\|\|\|__\|\|_\|\|_\|\|\|_\|\|\|\|\|\|\|\|\n______________UUGUG__CU_CA_UCU_UCGAUAAG\n3'-UUU...CAUGU_____AU_____G____________GUACC...GAA-5'\n_____________\|_________________________\|\n_____________46________________________22\n	221 -- 227	23 -- 29	-22.8159 kcal/mol	6.25755 kcal/mol	11.6121 kcal/mol	202 228@LB@ \| \|@LB@5'-UAC...GCAAU GU U G C GGAAC...AGG-3'@LB@ GACAC GA GU AGA AGCUAUUC@LB@ :\|\|\|\| \|\| \|\| \|\|\| \|\|\|\|\|\|\|\|@LB@ UUGUG CU CA UCU UCGAUAAG@LB@3'-UUU...CAUGU AU G GUACC...GAA-5'@LB@ \| \|@LB@ 46 22@LB@@LB@@LB@Energy -4.95 kcal/mol@LB@Hybridization Energy -22.8159 kcal/mol@LB@Unfolding Energy - mRNA 6.25755 kcal/mol@LB@Unfolding Energy - sRNA 11.6121 kcal/mol@LB@@LB@Position - mRNA 203 -- 227@LB@Position - sRNA 23 -- 45@LB@Position Seed - mRNA 221 -- 227@LB@Position Seed - sRNA 23 -- 29@LB@
94	0.03394	0.2959	ct_249	N/A	-6.52	0.182571	223 -- 229	80 -- 86		_____________222_____230\n_____________\|_______\|\n5'-UUG...UGAUC_______UUGGG...CUG-3'\n______________AUGGCUG\n______________\|:\|\|\|\|:\n______________UGCCGAU\n3'-UUU...CUCUA_______UGAGG...GAA-5'\n_____________\|_______\|\n_____________87______79\n	223 -- 229	80 -- 86	-8.72307 kcal/mol	1.13798 kcal/mol	1.06449 kcal/mol	222 230@LB@ \| \|@LB@5'-UUG...UGAUC UUGGG...CUG-3'@LB@ AUGGCUG@LB@ \|:\|\|\|\|:@LB@ UGCCGAU@LB@3'-UUU...CUCUA UGAGG...GAA-5'@LB@ \| \|@LB@ 87 79@LB@@LB@@LB@Energy -6.52 kcal/mol@LB@Hybridization Energy -8.72307 kcal/mol@LB@Unfolding Energy - mRNA 1.13798 kcal/mol@LB@Unfolding Energy - sRNA 1.06449 kcal/mol@LB@@LB@Position - mRNA 223 -- 229@LB@Position - sRNA 80 -- 86@LB@Position Seed - mRNA 223 -- 229@LB@Position Seed - sRNA 80 -- 86@LB@
95	0.03456	0.2959	ct_073	N/A	-12.13	0.007785	261 -- 279	41 -- 58	contains leader (19) peptide	_____________260_________________280\n_____________\|___________________\|\n5'-GAC...CUGAU_________AUU_______UGACA...GGG-3'\n______________CCCUCUGUG___CAAACAU\n______________\|\|\|:\|\|\|\|\|___\|\|\|\|\|\|:\n______________GGGGGACAC___GUUUGUG\n3'-UUU...UGAAG_________AU________AUCUC...GAA-5'\n_____________\|___________________\|\n_____________59__________________40\n	263 -- 269	50 -- 56	-22.185 kcal/mol	2.22773 kcal/mol	7.82865 kcal/mol	260 280@LB@ \| \|@LB@5'-GAC...CUGAU AUU UGACA...GGG-3'@LB@ CCCUCUGUG CAAACAU@LB@ \|\|\|:\|\|\|\|\| \|\|\|\|\|\|:@LB@ GGGGGACAC GUUUGUG@LB@3'-UUU...UGAAG AU AUCUC...GAA-5'@LB@ \| \|@LB@ 59 40@LB@@LB@@LB@Energy -12.13 kcal/mol@LB@Hybridization Energy -22.185 kcal/mol@LB@Unfolding Energy - mRNA 2.22773 kcal/mol@LB@Unfolding Energy - sRNA 7.82865 kcal/mol@LB@@LB@Position - mRNA 261 -- 279@LB@Position - sRNA 41 -- 58@LB@Position Seed - mRNA 263 -- 269@LB@Position Seed - sRNA 50 -- 56@LB@
96	0.03475	0.2959	ct_548	N/A	-8.10	0.081484	276 -- 282	54 -- 60		_____________275_____283\n_____________\|_______\|\n5'-ACA...UGCUC_______ACUAU...AAU-3'\n______________UCCCCCC\n______________\|\|\|\|\|\|\|\n______________AGGGGGG\n3'-UUU...ACUGA_______ACACA...GAA-5'\n_____________\|_______\|\n_____________61______53\n	276 -- 282	54 -- 60	-15.7273 kcal/mol	1.19056 kcal/mol	6.44182 kcal/mol	275 283@LB@ \| \|@LB@5'-ACA...UGCUC ACUAU...AAU-3'@LB@ UCCCCCC@LB@ \|\|\|\|\|\|\|@LB@ AGGGGGG@LB@3'-UUU...ACUGA ACACA...GAA-5'@LB@ \| \|@LB@ 61 53@LB@@LB@@LB@Energy -8.10 kcal/mol@LB@Hybridization Energy -15.7273 kcal/mol@LB@Unfolding Energy - mRNA 1.19056 kcal/mol@LB@Unfolding Energy - sRNA 6.44182 kcal/mol@LB@@LB@Position - mRNA 276 -- 282@LB@Position - sRNA 54 -- 60@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 54 -- 60@LB@
97	0.03496	0.2959	ct_539	trxA	-6.38	0.195495	87 -- 94	80 -- 87	thioredoxin	_____________86_______95\n_____________\|________\|\n5'-AUG...GAGGU________ACUGG...UGC-3'\n______________UAUGGCUA\n______________\|\|:\|\|\|\|\|\n______________AUGCCGAU\n3'-UUU...CCUCU________UGAGG...GAA-5'\n_____________\|________\|\n_____________88_______79\n	88 -- 94	80 -- 86	-10.4005 kcal/mol	2.9242 kcal/mol	1.09752 kcal/mol	86 95@LB@ \| \|@LB@5'-AUG...GAGGU ACUGG...UGC-3'@LB@ UAUGGCUA@LB@ \|\|:\|\|\|\|\|@LB@ AUGCCGAU@LB@3'-UUU...CCUCU UGAGG...GAA-5'@LB@ \| \|@LB@ 88 79@LB@@LB@@LB@Energy -6.38 kcal/mol@LB@Hybridization Energy -10.4005 kcal/mol@LB@Unfolding Energy - mRNA 2.9242 kcal/mol@LB@Unfolding Energy - sRNA 1.09752 kcal/mol@LB@@LB@Position - mRNA 87 -- 94@LB@Position - sRNA 80 -- 87@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 80 -- 86@LB@
98	0.03528	0.2959	ct_676	N/A	-6.99	0.144558	38 -- 44	80 -- 86	protein-arginine kinase activator protein	_____________37______45\n_____________\|_______\|\n5'-GGG...GCUCA_______GAGCA...GUU-3'\n______________GCGGUUA\n______________:\|\|\|:\|\|\n______________UGCCGAU\n3'-UUU...CUCUA_______UGAGG...GAA-5'\n_____________\|_______\|\n_____________87______79\n	38 -- 44	80 -- 86	-8.44953 kcal/mol	0.39201 kcal/mol	1.06449 kcal/mol	37 45@LB@ \| \|@LB@5'-GGG...GCUCA GAGCA...GUU-3'@LB@ GCGGUUA@LB@ :\|\|\|:\|\|@LB@ UGCCGAU@LB@3'-UUU...CUCUA UGAGG...GAA-5'@LB@ \| \|@LB@ 87 79@LB@@LB@@LB@Energy -6.99 kcal/mol@LB@Hybridization Energy -8.44953 kcal/mol@LB@Unfolding Energy - mRNA 0.39201 kcal/mol@LB@Unfolding Energy - sRNA 1.06449 kcal/mol@LB@@LB@Position - mRNA 38 -- 44@LB@Position - sRNA 80 -- 86@LB@Position Seed - mRNA 38 -- 44@LB@Position Seed - sRNA 80 -- 86@LB@
99	0.03647	0.3007	ct_810	rl32	-4.11	0.507281	35 -- 46	46 -- 58	50S ribosomal protein L32	_____________34____________47\n_____________\|_____________\|\n5'-UUC...CAAGU_____________CGUUU...GCA-3'\n______________CUUCCUG_GUAUA\n______________\|::\|\|\|\|_\|\|\|:\|\n______________GGGGGAC_CAUGU\n3'-UUU...UGAAG_______A_____UUGUG...GAA-5'\n_____________\|_____________\|\n_____________59____________45\n	35 -- 41	52 -- 58	-13.0221 kcal/mol	1.6119 kcal/mol	7.29598 kcal/mol	34 47@LB@ \| \|@LB@5'-UUC...CAAGU CGUUU...GCA-3'@LB@ CUUCCUG GUAUA@LB@ \|::\|\|\|\| \|\|\|:\|@LB@ GGGGGAC CAUGU@LB@3'-UUU...UGAAG A UUGUG...GAA-5'@LB@ \| \|@LB@ 59 45@LB@@LB@@LB@Energy -4.11 kcal/mol@LB@Hybridization Energy -13.0221 kcal/mol@LB@Unfolding Energy - mRNA 1.6119 kcal/mol@LB@Unfolding Energy - sRNA 7.29598 kcal/mol@LB@@LB@Position - mRNA 35 -- 46@LB@Position - sRNA 46 -- 58@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 52 -- 58@LB@
100	0.03658	0.3007	ct_875	N/A	-4.31	0.472369	272 -- 278	28 -- 34		_____________271_____279\n_____________\|_______\|\n5'-GGG...AAUCG_______CAAAA...AUA-3'\n______________CAGAAGU\n______________\|\|\|\|\|\|:\n______________GUCUUCG\n3'-UUU...UCUCA_______AUAAG...GAA-5'\n_____________\|_______\|\n_____________35______27\n	272 -- 278	28 -- 34	-8.98313 kcal/mol	0.5541 kcal/mol	4.11458 kcal/mol	271 279@LB@ \| \|@LB@5'-GGG...AAUCG CAAAA...AUA-3'@LB@ CAGAAGU@LB@ \|\|\|\|\|\|:@LB@ GUCUUCG@LB@3'-UUU...UCUCA AUAAG...GAA-5'@LB@ \| \|@LB@ 35 27@LB@@LB@@LB@Energy -4.31 kcal/mol@LB@Hybridization Energy -8.98313 kcal/mol@LB@Unfolding Energy - mRNA 0.5541 kcal/mol@LB@Unfolding Energy - sRNA 4.11458 kcal/mol@LB@@LB@Position - mRNA 272 -- 278@LB@Position - sRNA 28 -- 34@LB@Position Seed - mRNA 272 -- 278@LB@Position Seed - sRNA 28 -- 34@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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