Input and runtime details for job 7819033 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 7819033 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:17 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:46 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:05:24 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:05:32 CET 2018
	Job Completed		@ Fri Feb 16 21:05:57 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=7819033&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicC

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
135	0.01531	0.3774	b3224	nanT	-10.34	0.103537	56 -- 77	28 -- 54	sialic acid transporter	_____________55__________________________78\n_____________\|___________________________\|\n5'-CGC...CUGAA_____________________UU____CAAGA...GUU-3'\n______________GGC___GC__UGGCCCAGCAG__GAUG\n______________\|\|\|___\|\|__\|\|\|\|\|\|\|:\|\|\|__:\|\|:\n______________CCG___CG__ACCGGGUUGUC__UUAU\n3'-UUU...AGUCA___UUA__UU___________UU____UUUAG...UUG-5'\n_____________\|___________________________\|\n_____________55__________________________27\n	63 -- 69	36 -- 42	-28.0045 kcal/mol	8.68418 kcal/mol	8.98285 kcal/mol	55 78@LB@ \| \|@LB@5'-CGC...CUGAA UU CAAGA...GUU-3'@LB@ GGC GC UGGCCCAGCAG GAUG@LB@ \|\|\| \|\| \|\|\|\|\|\|\|:\|\|\| :\|\|:@LB@ CCG CG ACCGGGUUGUC UUAU@LB@3'-UUU...AGUCA UUA UU UU UUUAG...UUG-5'@LB@ \| \|@LB@ 55 27@LB@@LB@@LB@Energy -10.34 kcal/mol@LB@Hybridization Energy -28.0045 kcal/mol@LB@Unfolding Energy - mRNA 8.68418 kcal/mol@LB@Unfolding Energy - sRNA 8.98285 kcal/mol@LB@@LB@Position - mRNA 56 -- 77@LB@Position - sRNA 28 -- 54@LB@Position Seed - mRNA 63 -- 69@LB@Position Seed - sRNA 36 -- 42@LB@
161	0.01858	0.3864	b3237	argR	-5.47	0.658327	53 -- 70	1 -- 21	l-arginine-responsive arginine metabolism regulon transcriptional regulator	_____________52____________________71\n_____________\|_____________________\|\n5'-ACA...AUGCC______C___________UU_UCAAU...UGC-3'\n______________GUGACG____AGGCAUGU__C\n______________\|\|\|\|\|:____\|\|\|\|\|\|:\|__\|\n______________CACUGU____UCCGUAUA__G\n3'-UUU...UUAGA______UAUU________UU_-5'\n_____________\|____________________\|\n_____________22___________________1\n	61 -- 67	4 -- 10	-16.8661 kcal/mol	6.97949 kcal/mol	4.41334 kcal/mol	52 71@LB@ \| \|@LB@5'-ACA...AUGCC C UU UCAAU...UGC-3'@LB@ GUGACG AGGCAUGU C@LB@ \|\|\|\|\|: \|\|\|\|\|\|:\| \|@LB@ CACUGU UCCGUAUA G@LB@3'-UUU...UUAGA UAUU UU -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -5.47 kcal/mol@LB@Hybridization Energy -16.8661 kcal/mol@LB@Unfolding Energy - mRNA 6.97949 kcal/mol@LB@Unfolding Energy - sRNA 4.41334 kcal/mol@LB@@LB@Position - mRNA 53 -- 70@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 4 -- 10@LB@
12	0.0004505	0.1262	b3248	yhdE	-11.12	0.070056	184 -- 205	1 -- 21	dTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo-UTP pyrophosphatase	_____________183_____________________206\n_____________\|_______________________\|\n5'-UUA...UUGCA______________UUC______UUCUC...AGC-3'\n______________GUG_CAAUAAAGGU___UAUGAC\n______________\|\|\|_\|\|\|\|\|\|\|\|\|:___\|\|\|:\|\|\n______________CAC_GUUAUUUCCG___AUAUUG\n3'-UUU...UUAGA___U__________U________-5'\n_____________\|______________________\|\n_____________22_____________________1\n	190 -- 196	8 -- 14	-19.9684 kcal/mol	4.43203 kcal/mol	4.41334 kcal/mol	183 206@LB@ \| \|@LB@5'-UUA...UUGCA UUC UUCUC...AGC-3'@LB@ GUG CAAUAAAGGU UAUGAC@LB@ \|\|\| \|\|\|\|\|\|\|\|\|: \|\|\|:\|\|@LB@ CAC GUUAUUUCCG AUAUUG@LB@3'-UUU...UUAGA U U -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -11.12 kcal/mol@LB@Hybridization Energy -19.9684 kcal/mol@LB@Unfolding Energy - mRNA 4.43203 kcal/mol@LB@Unfolding Energy - sRNA 4.41334 kcal/mol@LB@@LB@Position - mRNA 184 -- 205@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 8 -- 14@LB@
183	0.02285	0.4143	b3262	yhdJ	-12.72	0.029953	3 -- 23	44 -- 69	DNA adenine methyltransferase SAM-dependent	_____________2__________________________24\n_____________\|__________________________\|\n_________5'-CC__________________________ACACC...GUG-3'\n______________UGUUGGA____CA_UGGUGAUGCAAU\n______________\|\|\|\|\|\|\|____\|\|_\|\|\|::\|\|\|\|\|\|\|\n______________ACAACCU____GU_ACCGUUACGUUA\n3'-UUU...AAUAU_______UUUA__C____________CCGGG...UUG-5'\n_____________\|__________________________\|\n_____________70_________________________43\n	17 -- 23	44 -- 50	-23.8856 kcal/mol	6.69358 kcal/mol	4.47358 kcal/mol	2 24@LB@ \| \|@LB@ 5'-CC ACACC...GUG-3'@LB@ UGUUGGA CA UGGUGAUGCAAU@LB@ \|\|\|\|\|\|\| \|\| \|\|\|::\|\|\|\|\|\|\|@LB@ ACAACCU GU ACCGUUACGUUA@LB@3'-UUU...AAUAU UUUA C CCGGG...UUG-5'@LB@ \| \|@LB@ 70 43@LB@@LB@@LB@Energy -12.72 kcal/mol@LB@Hybridization Energy -23.8856 kcal/mol@LB@Unfolding Energy - mRNA 6.69358 kcal/mol@LB@Unfolding Energy - sRNA 4.47358 kcal/mol@LB@@LB@Position - mRNA 3 -- 23@LB@Position - sRNA 44 -- 69@LB@Position Seed - mRNA 17 -- 23@LB@Position Seed - sRNA 44 -- 50@LB@
74	0.006275	0.2822	b3298	rpsM	-14.03	0.014091	187 -- 207	39 -- 57	30S ribosomal subunit protein S13	_____________186___________________208\n_____________\|_____________________\|\n5'-GUC...UAAAU___A_G______UA_______GUAUA...UCC-3'\n______________AGU_G_AGUGCA__GUGGCCC\n______________\|\|\|_\|_\|:\|\|\|\|__:\|\|\|\|\|\|\n______________UCA_C_UUACGU__UACCGGG\n3'-UUU...UUUAG___C_G_______________UUGUC...UUG-5'\n_____________\|_____________________\|\n_____________58____________________38\n	201 -- 207	39 -- 45	-23.7565 kcal/mol	4.54468 kcal/mol	5.17956 kcal/mol	186 208@LB@ \| \|@LB@5'-GUC...UAAAU A G UA GUAUA...UCC-3'@LB@ AGU G AGUGCA GUGGCCC@LB@ \|\|\| \| \|:\|\|\|\| :\|\|\|\|\|\|@LB@ UCA C UUACGU UACCGGG@LB@3'-UUU...UUUAG C G UUGUC...UUG-5'@LB@ \| \|@LB@ 58 38@LB@@LB@@LB@Energy -14.03 kcal/mol@LB@Hybridization Energy -23.7565 kcal/mol@LB@Unfolding Energy - mRNA 4.54468 kcal/mol@LB@Unfolding Energy - sRNA 5.17956 kcal/mol@LB@@LB@Position - mRNA 187 -- 207@LB@Position - sRNA 39 -- 57@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 39 -- 45@LB@
197	0.02537	0.4256	b3317	rplB	-11.01	0.074049	182 -- 205	41 -- 62	50S ribosomal subunit protein L2	_____________181______________________206\n_____________\|________________________\|\n5'-GCA...UGAGU_______AG_A_____A_______AGUUG...UGC-3'\n______________AAGUCGG__G_GUAAU_CAAUGGC\n______________\|\|:\|\|:\|__\|_\|:\|\|\|_\|\|\|\|\|\|\|\n______________UUUAGUC__C_CGUUA_GUUACCG\n3'-UUU...AACCU_______A________C_______GGUUG...UUG-5'\n_____________\|________________________\|\n_____________63_______________________40\n	199 -- 205	41 -- 47	-19.8959 kcal/mol	4.6063 kcal/mol	4.27585 kcal/mol	181 206@LB@ \| \|@LB@5'-GCA...UGAGU AG A A AGUUG...UGC-3'@LB@ AAGUCGG G GUAAU CAAUGGC@LB@ \|\|:\|\|:\| \| \|:\|\|\| \|\|\|\|\|\|\|@LB@ UUUAGUC C CGUUA GUUACCG@LB@3'-UUU...AACCU A C GGUUG...UUG-5'@LB@ \| \|@LB@ 63 40@LB@@LB@@LB@Energy -11.01 kcal/mol@LB@Hybridization Energy -19.8959 kcal/mol@LB@Unfolding Energy - mRNA 4.6063 kcal/mol@LB@Unfolding Energy - sRNA 4.27585 kcal/mol@LB@@LB@Position - mRNA 182 -- 205@LB@Position - sRNA 41 -- 62@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 41 -- 47@LB@
45	0.002879	0.2151	b3319	rplD	-8.41	0.246804	191 -- 204	42 -- 54	50S ribosomal subunit protein L4	_____________190____________205\n_____________\|______________\|\n5'-UCA...CGUAA__A___A_______AAUUA...UUG-3'\n______________GG_GAU_GCAAUGG\n______________\|\|_:\|\|_\|\|\|\|\|\|\|\n______________CC_UUA_CGUUACC\n3'-UUU...AGUCA__G___________GGGUU...UUG-5'\n_____________\|______________\|\n_____________55_____________41\n	198 -- 204	42 -- 48	-13.9027 kcal/mol	1.99374 kcal/mol	3.49932 kcal/mol	190 205@LB@ \| \|@LB@5'-UCA...CGUAA A A AAUUA...UUG-3'@LB@ GG GAU GCAAUGG@LB@ \|\| :\|\| \|\|\|\|\|\|\|@LB@ CC UUA CGUUACC@LB@3'-UUU...AGUCA G GGGUU...UUG-5'@LB@ \| \|@LB@ 55 41@LB@@LB@@LB@Energy -8.41 kcal/mol@LB@Hybridization Energy -13.9027 kcal/mol@LB@Unfolding Energy - mRNA 1.99374 kcal/mol@LB@Unfolding Energy - sRNA 3.49932 kcal/mol@LB@@LB@Position - mRNA 191 -- 204@LB@Position - sRNA 42 -- 54@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 42 -- 48@LB@
77	0.006658	0.2822	b3347	fkpA	-8.74	0.214444	80 -- 111	4 -- 28	FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase)	_____________79________________________________112\n_____________\|_________________________________\|\n5'-UGG...CAAAA________AAAAUAGA_________________CUGUA...CAG-3'\n______________AGAGUCUG________UGAUAAU_AGGGCGUGU\n______________\|:\|:\|\|\|\|________\|\|\|:\|\|\|_\|:\|\|\|:\|:\|\n______________UUUUAGAC________ACUGUUA_UUCCGUAUA\n3'-UUU...UUUUA_______________________U_________UUG-5'\n_____________\|_________________________________\|\n_____________29________________________________3\n	104 -- 110	5 -- 11	-22.6947 kcal/mol	9.05802 kcal/mol	4.89231 kcal/mol	79 112@LB@ \| \|@LB@5'-UGG...CAAAA AAAAUAGA CUGUA...CAG-3'@LB@ AGAGUCUG UGAUAAU AGGGCGUGU@LB@ \|:\|:\|\|\|\| \|\|\|:\|\|\| \|:\|\|\|:\|:\|@LB@ UUUUAGAC ACUGUUA UUCCGUAUA@LB@3'-UUU...UUUUA U UUG-5'@LB@ \| \|@LB@ 29 3@LB@@LB@@LB@Energy -8.74 kcal/mol@LB@Hybridization Energy -22.6947 kcal/mol@LB@Unfolding Energy - mRNA 9.05802 kcal/mol@LB@Unfolding Energy - sRNA 4.89231 kcal/mol@LB@@LB@Position - mRNA 80 -- 111@LB@Position - sRNA 4 -- 28@LB@Position Seed - mRNA 104 -- 110@LB@Position Seed - sRNA 5 -- 11@LB@
147	0.01713	0.3828	b3354	yheU	-14.93	0.008191	201 -- 276	7 -- 70	UPF0270 family protein	_____________200_____________________________________________________________________________277\n_____________\|_______________________________________________________________________________\|\n5'-CUG...AAAUC______AU_____C_______GACCUCUCC_____AACGC________UU____GAAAGCUU____UUAC_________CCGAU...GUA-3'\n______________AUGUUG_____UC_GUGGCAA_________CCCGA_____UGGAAAAU__AAUU________UGUG____GUGAAGGCA\n______________\|\|\|\|\|\|_____\|\|_\|\|\|\|\|\|\|_________\|\|\|:\|_____::\|\|\|\|\|\|__\|\|\|:________\|\|\|\|____:\|:\|\|\|\|\|\|\n______________UACAAC_____AG_CACCGUU_________GGGUU_____GUCUUUUA__UUAG________ACAC____UAUUUCCGU\n3'-UUU...AAAUA______CUUUU__U_______ACGUUACC___________________UU________________UGU__________AUAUUG-5'\n_____________\|_______________________________________________________________________________\|\n_____________71______________________________________________________________________________6\n	270 -- 276	7 -- 13	-40.9535 kcal/mol	13.8654 kcal/mol	12.1606 kcal/mol	200 277@LB@ \| \|@LB@5'-CUG...AAAUC AU C GACCUCUCC AACGC UU GAAAGCUU UUAC CCGAU...GUA-3'@LB@ AUGUUG UC GUGGCAA CCCGA UGGAAAAU AAUU UGUG GUGAAGGCA@LB@ \|\|\|\|\|\| \|\| \|\|\|\|\|\|\| \|\|\|:\| ::\|\|\|\|\|\| \|\|\|: \|\|\|\| :\|:\|\|\|\|\|\|@LB@ UACAAC AG CACCGUU GGGUU GUCUUUUA UUAG ACAC UAUUUCCGU@LB@3'-UUU...AAAUA CUUUU U ACGUUACC UU UGU AUAUUG-5'@LB@ \| \|@LB@ 71 6@LB@@LB@@LB@Energy -14.93 kcal/mol@LB@Hybridization Energy -40.9535 kcal/mol@LB@Unfolding Energy - mRNA 13.8654 kcal/mol@LB@Unfolding Energy - sRNA 12.1606 kcal/mol@LB@@LB@Position - mRNA 201 -- 276@LB@Position - sRNA 7 -- 70@LB@Position Seed - mRNA 270 -- 276@LB@Position Seed - sRNA 7 -- 13@LB@
91	0.007817	0.2889	b3380	yhfW	-10.82	0.081759	179 -- 205	41 -- 67	phosphopentomutase-related metalloenzyme superfamily protein	_____________178_________________________206\n_____________\|___________________________\|\n5'-CUC...CAGUC_______C__UC___G__C________GCGAU...GUG-3'\n______________UUGGAAA_UC__UGG_GG_GUAAUGGC\n______________\|\|\|\|\|\|\|_\|\|__\|\|\|_::_\|:\|\|\|\|\|\|\n______________AACCUUU_AG__ACC_UU_CGUUACCG\n3'-UUU...UAUAC_______U__UC___G__A________GGUUG...UUG-5'\n_____________\|___________________________\|\n_____________68__________________________40\n	199 -- 205	41 -- 47	-26.7792 kcal/mol	10.1319 kcal/mol	5.83043 kcal/mol	178 206@LB@ \| \|@LB@5'-CUC...CAGUC C UC G C GCGAU...GUG-3'@LB@ UUGGAAA UC UGG GG GUAAUGGC@LB@ \|\|\|\|\|\|\| \|\| \|\|\| :: \|:\|\|\|\|\|\|@LB@ AACCUUU AG ACC UU CGUUACCG@LB@3'-UUU...UAUAC U UC G A GGUUG...UUG-5'@LB@ \| \|@LB@ 68 40@LB@@LB@@LB@Energy -10.82 kcal/mol@LB@Hybridization Energy -26.7792 kcal/mol@LB@Unfolding Energy - mRNA 10.1319 kcal/mol@LB@Unfolding Energy - sRNA 5.83043 kcal/mol@LB@@LB@Position - mRNA 179 -- 205@LB@Position - sRNA 41 -- 67@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 41 -- 47@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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