Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
51	0.002326	0.1469	b2780	pyrG	-6.37	0.158020	19 -- 28	33 -- 42	CTP synthetase	_____________18_________29\n_____________\|__________\|\n5'-UGU...AUGAC__________UUUGC...GCC-3'\n______________AAGCGCUUGA\n______________\|\|\|\|:\|\|\|:\|\n______________UUCGUGAAUU\n3'-UUU...CGUUC__________UUUUA...CAA-5'\n_____________\|__________\|\n_____________43_________32\n	22 -- 28	33 -- 39	-10.9983 kcal/mol	3.00927 kcal/mol	1.61944 kcal/mol	18 29@LB@ \| \|@LB@5'-UGU...AUGAC UUUGC...GCC-3'@LB@ AAGCGCUUGA@LB@ \|\|\|\|:\|\|\|:\|@LB@ UUCGUGAAUU@LB@3'-UUU...CGUUC UUUUA...CAA-5'@LB@ \| \|@LB@ 43 32@LB@@LB@@LB@Energy -6.37 kcal/mol@LB@Hybridization Energy -10.9983 kcal/mol@LB@Unfolding Energy - mRNA 3.00927 kcal/mol@LB@Unfolding Energy - sRNA 1.61944 kcal/mol@LB@@LB@Position - mRNA 19 -- 28@LB@Position - sRNA 33 -- 42@LB@Position Seed - mRNA 22 -- 28@LB@Position Seed - sRNA 33 -- 39@LB@
30	0.0008402	0.09021	b2792	yqcC	-12.92	0.001907	10 -- 25	38 -- 53	DUF446 family protein	_____________9________________26\n_____________\|________________\|\n5'-AUC...GGGUG__G_____________AUAGU...AAU-3'\n______________GU_UGAGCAGGAAGCA\n______________\|:_\|:\|\|\|\|:\|\|\|\|\|\|\n______________CG_AUUCGUUCUUCGU\n3'-UUU...UUGAA__A_____________GAAUU...CAA-5'\n_____________\|________________\|\n_____________54_______________37\n	19 -- 25	38 -- 44	-21.2799 kcal/mol	2.27149 kcal/mol	6.09301 kcal/mol	9 26@LB@ \| \|@LB@5'-AUC...GGGUG G AUAGU...AAU-3'@LB@ GU UGAGCAGGAAGCA@LB@ \|: \|:\|\|\|\|:\|\|\|\|\|\|@LB@ CG AUUCGUUCUUCGU@LB@3'-UUU...UUGAA A GAAUU...CAA-5'@LB@ \| \|@LB@ 54 37@LB@@LB@@LB@Energy -12.92 kcal/mol@LB@Hybridization Energy -21.2799 kcal/mol@LB@Unfolding Energy - mRNA 2.27149 kcal/mol@LB@Unfolding Energy - sRNA 6.09301 kcal/mol@LB@@LB@Position - mRNA 10 -- 25@LB@Position - sRNA 38 -- 53@LB@Position Seed - mRNA 19 -- 25@LB@Position Seed - sRNA 38 -- 44@LB@
29	0.000834	0.09021	b2798	ygdG	-11.41	0.005967	211 -- 245	24 -- 57	Ssb-binding protein misidentified as ExoIX	_____________210_________________________________246\n_____________\|___________________________________\|\n5'-CCA...UGUUC_________UUGUC__U_____GAAUCUU______CGCAU...UCG-3'\n______________AUUUGCUUA_____GA_GCACU_______AUUCGU\n______________\|:\|\|\|\|\|\|\|_____\|\|_\|\|\|\|\|_______\|\|\|\|\|\|\n______________UGAACGAAU_____CU_CGUGA_______UAAGCA\n3'-UUU...UACUU_________UCGUU__U_____AUUUUU_______AUGUG...CAA-5'\n_____________\|___________________________________\|\n_____________58__________________________________23\n	213 -- 219	49 -- 55	-23.2646 kcal/mol	5.57825 kcal/mol	6.27607 kcal/mol	210 246@LB@ \| \|@LB@5'-CCA...UGUUC UUGUC U GAAUCUU CGCAU...UCG-3'@LB@ AUUUGCUUA GA GCACU AUUCGU@LB@ \|:\|\|\|\|\|\|\| \|\| \|\|\|\|\| \|\|\|\|\|\|@LB@ UGAACGAAU CU CGUGA UAAGCA@LB@3'-UUU...UACUU UCGUU U AUUUUU AUGUG...CAA-5'@LB@ \| \|@LB@ 58 23@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -23.2646 kcal/mol@LB@Unfolding Energy - mRNA 5.57825 kcal/mol@LB@Unfolding Energy - sRNA 6.27607 kcal/mol@LB@@LB@Position - mRNA 211 -- 245@LB@Position - sRNA 24 -- 57@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 49 -- 55@LB@
165	0.01827	0.3566	b2937	speB	-7.30	0.092452	5 -- 22	25 -- 41	agmatinase	_____________4__________________23\n_____________\|__________________\|\n_______5'-CAAC___UC_____________AGGCA...CAG-3'\n______________AGU__CUUGAAGAGUUCG\n______________\|\|:__\|\|\|:\|\|:\|:\|\|\|\|\n______________UCG__GAAUUUUUUAAGC\n3'-UUU...GUUCU___U______________AAUGU...CAA-5'\n_____________\|__________________\|\n_____________42_________________24\n	16 -- 22	25 -- 31	-14.9284 kcal/mol	5.98061 kcal/mol	1.64839 kcal/mol	4 23@LB@ \| \|@LB@ 5'-CAAC UC AGGCA...CAG-3'@LB@ AGU CUUGAAGAGUUCG@LB@ \|\|: \|\|\|:\|\|:\|:\|\|\|\|@LB@ UCG GAAUUUUUUAAGC@LB@3'-UUU...GUUCU U AAUGU...CAA-5'@LB@ \| \|@LB@ 42 24@LB@@LB@@LB@Energy -7.30 kcal/mol@LB@Hybridization Energy -14.9284 kcal/mol@LB@Unfolding Energy - mRNA 5.98061 kcal/mol@LB@Unfolding Energy - sRNA 1.64839 kcal/mol@LB@@LB@Position - mRNA 5 -- 22@LB@Position - sRNA 25 -- 41@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 25 -- 31@LB@
130	0.01234	0.3033	b2958	yggN	-5.61	0.236844	227 -- 233	35 -- 41	DUF2884 family putative periplasmic protein	_____________226_____234\n_____________\|_______\|\n5'-ACG...GCGGC_______UCAGU...UUG-3'\n______________AGCACUU\n______________\|\|\|\|\|\|\|\n______________UCGUGAA\n3'-UUU...GUUCU_______UUUUU...CAA-5'\n_____________\|_______\|\n_____________42______34\n	227 -- 233	35 -- 41	-8.1936 kcal/mol	0.976472 kcal/mol	1.6033 kcal/mol	226 234@LB@ \| \|@LB@5'-ACG...GCGGC UCAGU...UUG-3'@LB@ AGCACUU@LB@ \|\|\|\|\|\|\|@LB@ UCGUGAA@LB@3'-UUU...GUUCU UUUUU...CAA-5'@LB@ \| \|@LB@ 42 34@LB@@LB@@LB@Energy -5.61 kcal/mol@LB@Hybridization Energy -8.1936 kcal/mol@LB@Unfolding Energy - mRNA 0.976472 kcal/mol@LB@Unfolding Energy - sRNA 1.6033 kcal/mol@LB@@LB@Position - mRNA 227 -- 233@LB@Position - sRNA 35 -- 41@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 35 -- 41@LB@
107	0.009263	0.2693	b3034	nudF	-7.86	0.065629	202 -- 214	42 -- 54	ADP-ribose pyrophosphatase	_____________201___________215\n_____________\|_____________\|\n5'-AUU...AACAA_________C___CAACC...UUU-3'\n______________UGCUUAAGC_AGA\n______________\|\|\|\|\|\|\|\|\|_\|\|\|\n______________ACGAAUUCG_UCU\n3'-UUU...UUUGA_________U___UCGUG...CAA-5'\n_____________\|_____________\|\n_____________55____________41\n	204 -- 210	46 -- 52	-15.7149 kcal/mol	1.83586 kcal/mol	6.01534 kcal/mol	201 215@LB@ \| \|@LB@5'-AUU...AACAA C CAACC...UUU-3'@LB@ UGCUUAAGC AGA@LB@ \|\|\|\|\|\|\|\|\| \|\|\|@LB@ ACGAAUUCG UCU@LB@3'-UUU...UUUGA U UCGUG...CAA-5'@LB@ \| \|@LB@ 55 41@LB@@LB@@LB@Energy -7.86 kcal/mol@LB@Hybridization Energy -15.7149 kcal/mol@LB@Unfolding Energy - mRNA 1.83586 kcal/mol@LB@Unfolding Energy - sRNA 6.01534 kcal/mol@LB@@LB@Position - mRNA 202 -- 214@LB@Position - sRNA 42 -- 54@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 46 -- 52@LB@
187	0.02188	0.3768	b3065	rpsU	-8.54	0.042773	188 -- 200	37 -- 49	30S ribosomal subunit protein S21	_____________187___________201\n_____________\|_____________\|\n5'-GAC...AUCAA_____________AUGCC...GUC-3'\n______________AGGUGAGAGGCAC\n______________\|:\|::\|\|\|:\|\|\|\|\n______________UUCGUUCUUCGUG\n3'-UUU...ACGAA_____________AAUUU...CAA-5'\n_____________\|_____________\|\n_____________50____________36\n	194 -- 200	37 -- 43	-18.635 kcal/mol	4.05763 kcal/mol	6.03369 kcal/mol	187 201@LB@ \| \|@LB@5'-GAC...AUCAA AUGCC...GUC-3'@LB@ AGGUGAGAGGCAC@LB@ \|:\|::\|\|\|:\|\|\|\|@LB@ UUCGUUCUUCGUG@LB@3'-UUU...ACGAA AAUUU...CAA-5'@LB@ \| \|@LB@ 50 36@LB@@LB@@LB@Energy -8.54 kcal/mol@LB@Hybridization Energy -18.635 kcal/mol@LB@Unfolding Energy - mRNA 4.05763 kcal/mol@LB@Unfolding Energy - sRNA 6.03369 kcal/mol@LB@@LB@Position - mRNA 188 -- 200@LB@Position - sRNA 37 -- 49@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 37 -- 43@LB@
87	0.005861	0.217	b3083	higB	-4.01	0.492619	220 -- 233	28 -- 42	mRNA interferase toxin of the HigB-HigA toxin-antitoxin system	_____________219_____________234\n_____________\|_______________\|\n5'-GCA...UCAAA_______________GCUGC...GAU-3'\n______________AAGCAUU_GAAAGAU\n______________\|\|\|\|\|:\|_:\|\|\|:\|\|\n______________UUCGUGA_UUUUUUA\n3'-UUU...CGUUC_______A_______AGCAA...CAA-5'\n_____________\|_______________\|\n_____________43______________27\n	220 -- 226	36 -- 42	-9.16043 kcal/mol	3.51035 kcal/mol	1.63711 kcal/mol	219 234@LB@ \| \|@LB@5'-GCA...UCAAA GCUGC...GAU-3'@LB@ AAGCAUU GAAAGAU@LB@ \|\|\|\|\|:\| :\|\|\|:\|\|@LB@ UUCGUGA UUUUUUA@LB@3'-UUU...CGUUC A AGCAA...CAA-5'@LB@ \| \|@LB@ 43 27@LB@@LB@@LB@Energy -4.01 kcal/mol@LB@Hybridization Energy -9.16043 kcal/mol@LB@Unfolding Energy - mRNA 3.51035 kcal/mol@LB@Unfolding Energy - sRNA 1.63711 kcal/mol@LB@@LB@Position - mRNA 220 -- 233@LB@Position - sRNA 28 -- 42@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 36 -- 42@LB@
46	0.002046	0.1433	b3106	yhaK	-10.10	0.015122	97 -- 120	7 -- 28	redox-sensitive bicupin	_____________96_______________________121\n_____________\|________________________\|\n5'-UCC...GCCAU______CU__U_____________ACAUA...AAU-3'\n______________UUCGUU__CA_UCAGGAAAUUUGA\n______________\|\|\|\|\|\|__\|\|_:\|\|\|\|\|\|\|\|:\|\|\|\n______________AAGCAA__GU_GGUCCUUUAGACU\n3'-UUU...UUUUU______U_________________ACACAA-5'\n_____________\|________________________\|\n_____________29_______________________6\n	111 -- 117	10 -- 16	-23.1033 kcal/mol	4.40095 kcal/mol	8.59864 kcal/mol	96 121@LB@ \| \|@LB@5'-UCC...GCCAU CU U ACAUA...AAU-3'@LB@ UUCGUU CA UCAGGAAAUUUGA@LB@ \|\|\|\|\|\| \|\| :\|\|\|\|\|\|\|\|:\|\|\|@LB@ AAGCAA GU GGUCCUUUAGACU@LB@3'-UUU...UUUUU U ACACAA-5'@LB@ \| \|@LB@ 29 6@LB@@LB@@LB@Energy -10.10 kcal/mol@LB@Hybridization Energy -23.1033 kcal/mol@LB@Unfolding Energy - mRNA 4.40095 kcal/mol@LB@Unfolding Energy - sRNA 8.59864 kcal/mol@LB@@LB@Position - mRNA 97 -- 120@LB@Position - sRNA 7 -- 28@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 10 -- 16@LB@
177	0.02045	0.3721	b3148	yraN	-6.85	0.120614	263 -- 276	37 -- 54	UPF0102 family protein	_____________262________________277\n_____________\|__________________\|\n5'-GGU...GGCGA__________________AAGCG...GCA-3'\n______________UGC____GUGGGAAGCAC\n______________\|\|\|____\|:::\|\|\|\|\|\|\|\n______________ACG____CGUUCUUCGUG\n3'-UUU...UUUGA___AAUU___________AAUUU...CAA-5'\n_____________\|__________________\|\n_____________55_________________36\n	270 -- 276	37 -- 43	-17.1481 kcal/mol	4.14332 kcal/mol	6.1584 kcal/mol	262 277@LB@ \| \|@LB@5'-GGU...GGCGA AAGCG...GCA-3'@LB@ UGC GUGGGAAGCAC@LB@ \|\|\| \|:::\|\|\|\|\|\|\|@LB@ ACG CGUUCUUCGUG@LB@3'-UUU...UUUGA AAUU AAUUU...CAA-5'@LB@ \| \|@LB@ 55 36@LB@@LB@@LB@Energy -6.85 kcal/mol@LB@Hybridization Energy -17.1481 kcal/mol@LB@Unfolding Energy - mRNA 4.14332 kcal/mol@LB@Unfolding Energy - sRNA 6.1584 kcal/mol@LB@@LB@Position - mRNA 263 -- 276@LB@Position - sRNA 37 -- 54@LB@Position Seed - mRNA 270 -- 276@LB@Position Seed - sRNA 37 -- 43@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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