Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
159	0.01739	0.3504	b2426	ucpA	-8.50	0.043854	215 -- 229	36 -- 49	furfural resistance protein putative short-chain oxidoreductase	_____________214_____________230\n_____________\|_______________\|\n5'-GUG...CUCAC________C______GAUUA...UAA-3'\n______________GGGCAAGA_AGCACU\n______________::\|\|\|\|\|\|_\|\|\|\|\|\|\n______________UUCGUUCU_UCGUGA\n3'-UUU...ACGAA_______________AUUUU...CAA-5'\n_____________\|_______________\|\n_____________50______________35\n	216 -- 222	42 -- 48	-19.0239 kcal/mol	4.4828 kcal/mol	6.03645 kcal/mol	214 230@LB@ \| \|@LB@5'-GUG...CUCAC C GAUUA...UAA-3'@LB@ GGGCAAGA AGCACU@LB@ ::\|\|\|\|\|\| \|\|\|\|\|\|@LB@ UUCGUUCU UCGUGA@LB@3'-UUU...ACGAA AUUUU...CAA-5'@LB@ \| \|@LB@ 50 35@LB@@LB@@LB@Energy -8.50 kcal/mol@LB@Hybridization Energy -19.0239 kcal/mol@LB@Unfolding Energy - mRNA 4.4828 kcal/mol@LB@Unfolding Energy - sRNA 6.03645 kcal/mol@LB@@LB@Position - mRNA 215 -- 229@LB@Position - sRNA 36 -- 49@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 42 -- 48@LB@
133	0.0127	0.3077	b2452	eutH	-4.80	0.352106	186 -- 194	38 -- 46	ethanolamine transporter	_____________185_______195\n_____________\|_________\|\n5'-UUG...ACUAA_________AAGGC...UCC-3'\n______________CAGGGAGUA\n______________\|\|:\|:\|\|:\|\n______________GUUCUUCGU\n3'-UUU...AAUUC_________GAAUU...CAA-5'\n_____________\|_________\|\n_____________47________37\n	188 -- 194	38 -- 44	-8.62764 kcal/mol	1.2009 kcal/mol	2.62609 kcal/mol	185 195@LB@ \| \|@LB@5'-UUG...ACUAA AAGGC...UCC-3'@LB@ CAGGGAGUA@LB@ \|\|:\|:\|\|:\|@LB@ GUUCUUCGU@LB@3'-UUU...AAUUC GAAUU...CAA-5'@LB@ \| \|@LB@ 47 37@LB@@LB@@LB@Energy -4.80 kcal/mol@LB@Hybridization Energy -8.62764 kcal/mol@LB@Unfolding Energy - mRNA 1.2009 kcal/mol@LB@Unfolding Energy - sRNA 2.62609 kcal/mol@LB@@LB@Position - mRNA 186 -- 194@LB@Position - sRNA 38 -- 46@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 38 -- 44@LB@
112	0.009574	0.2693	b2494	bepA	-6.13	0.179960	208 -- 220	29 -- 41	OM protein maintenance and assembly metalloprotease and chaperone periplasmic	_____________207___________221\n_____________\|_____________\|\n5'-UGA...GUUCA____G________AAACC...UGC-3'\n______________GGCA_UUGAAAAA\n______________:\|\|\|_\|\|:\|\|\|\|\|\n______________UCGU_AAUUUUUU\n3'-UUU...GUUCU____G________AAGCA...CAA-5'\n_____________\|_____________\|\n_____________42____________28\n	214 -- 220	29 -- 35	-11.3192 kcal/mol	3.5632 kcal/mol	1.62306 kcal/mol	207 221@LB@ \| \|@LB@5'-UGA...GUUCA G AAACC...UGC-3'@LB@ GGCA UUGAAAAA@LB@ :\|\|\| \|\|:\|\|\|\|\|@LB@ UCGU AAUUUUUU@LB@3'-UUU...GUUCU G AAGCA...CAA-5'@LB@ \| \|@LB@ 42 28@LB@@LB@@LB@Energy -6.13 kcal/mol@LB@Hybridization Energy -11.3192 kcal/mol@LB@Unfolding Energy - mRNA 3.5632 kcal/mol@LB@Unfolding Energy - sRNA 1.62306 kcal/mol@LB@@LB@Position - mRNA 208 -- 220@LB@Position - sRNA 29 -- 41@LB@Position Seed - mRNA 214 -- 220@LB@Position Seed - sRNA 29 -- 35@LB@
171	0.01957	0.3668	b2498	upp	-5.84	0.210430	244 -- 265	42 -- 61	uracil phosphoribosyltransferase	_____________243____________________266\n_____________\|______________________\|\n5'-GGC...CAAGC_______GA___G_________UAUCA...CCG-3'\n______________UGGGACU__UGC_UGAGCAAGA\n______________\|\|::\|\|\|__\|\|\|_\|:\|\|\|\|\|\|\|\n______________ACUUUGA__ACG_AUUCGUUCU\n3'-UUU...GCCCU____________A_________UCGUG...CAA-5'\n_____________\|______________________\|\n_____________62_____________________41\n	259 -- 265	42 -- 48	-21.1169 kcal/mol	8.03919 kcal/mol	7.23689 kcal/mol	243 266@LB@ \| \|@LB@5'-GGC...CAAGC GA G UAUCA...CCG-3'@LB@ UGGGACU UGC UGAGCAAGA@LB@ \|\|::\|\|\| \|\|\| \|:\|\|\|\|\|\|\|@LB@ ACUUUGA ACG AUUCGUUCU@LB@3'-UUU...GCCCU A UCGUG...CAA-5'@LB@ \| \|@LB@ 62 41@LB@@LB@@LB@Energy -5.84 kcal/mol@LB@Hybridization Energy -21.1169 kcal/mol@LB@Unfolding Energy - mRNA 8.03919 kcal/mol@LB@Unfolding Energy - sRNA 7.23689 kcal/mol@LB@@LB@Position - mRNA 244 -- 265@LB@Position - sRNA 42 -- 61@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 42 -- 48@LB@
56	0.002803	0.1597	b2514	hisS	-5.93	0.201249	279 -- 295	24 -- 41	histidyl tRNA synthetase	_____________278_________________296\n_____________\|___________________\|\n5'-AUG...UUGAA_____AC____________GCUCG-3'\n______________GGCAC__UGAAAAA__CGU\n______________:\|\|\|\|__\|:\|\|\|\|\|__\|\|\|\n______________UCGUG__AUUUUUU__GCA\n3'-UUU...GUUCU_____A________AA___AUGUG...CAA-5'\n_____________\|___________________\|\n_____________42__________________23\n	286 -- 292	29 -- 35	-10.1195 kcal/mol	2.54562 kcal/mol	1.64881 kcal/mol	278 296@LB@ \| \|@LB@5'-AUG...UUGAA AC GCUCG-3'@LB@ GGCAC UGAAAAA CGU@LB@ :\|\|\|\| \|:\|\|\|\|\| \|\|\|@LB@ UCGUG AUUUUUU GCA@LB@3'-UUU...GUUCU A AA AUGUG...CAA-5'@LB@ \| \|@LB@ 42 23@LB@@LB@@LB@Energy -5.93 kcal/mol@LB@Hybridization Energy -10.1195 kcal/mol@LB@Unfolding Energy - mRNA 2.54562 kcal/mol@LB@Unfolding Energy - sRNA 1.64881 kcal/mol@LB@@LB@Position - mRNA 279 -- 295@LB@Position - sRNA 24 -- 41@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 29 -- 35@LB@
132	0.01257	0.3067	b2516	rodZ	-8.98	0.032240	231 -- 240	34 -- 43	MreB assembly cytoskeletal protein	_____________230________241\n_____________\|__________\|\n5'-UAA...AAAAU__________CUACC...UUG-3'\n______________GAAGCACUUA\n______________\|\|\|\|\|\|\|\|\|\|\n______________CUUCGUGAAU\n3'-UUU...UCGUU__________UUUUU...CAA-5'\n_____________\|__________\|\n_____________44_________33\n	234 -- 240	34 -- 40	-13.7965 kcal/mol	1.10051 kcal/mol	3.71643 kcal/mol	230 241@LB@ \| \|@LB@5'-UAA...AAAAU CUACC...UUG-3'@LB@ GAAGCACUUA@LB@ \|\|\|\|\|\|\|\|\|\|@LB@ CUUCGUGAAU@LB@3'-UUU...UCGUU UUUUU...CAA-5'@LB@ \| \|@LB@ 44 33@LB@@LB@@LB@Energy -8.98 kcal/mol@LB@Hybridization Energy -13.7965 kcal/mol@LB@Unfolding Energy - mRNA 1.10051 kcal/mol@LB@Unfolding Energy - sRNA 3.71643 kcal/mol@LB@@LB@Position - mRNA 231 -- 240@LB@Position - sRNA 34 -- 43@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 34 -- 40@LB@
24	0.000694	0.09021	b2531	iscR	-7.21	0.097486	53 -- 65	27 -- 41	isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress- and iron starvation-inducible; autorepressor	_____________52______________66\n_____________\|_______________\|\n5'-AUG...AAAAU_______________AAAUA...AAC-3'\n______________GGCACU__GAAGGUU\n______________:\|\|\|\|\|__:\|\|::\|\|\n______________UCGUGA__UUUUUAA\n3'-UUU...GUUCU______AU_______GCAAU...CAA-5'\n_____________\|_______________\|\n_____________42______________26\n	59 -- 65	27 -- 33	-10.1429 kcal/mol	1.29 kcal/mol	1.6427 kcal/mol	52 66@LB@ \| \|@LB@5'-AUG...AAAAU AAAUA...AAC-3'@LB@ GGCACU GAAGGUU@LB@ :\|\|\|\|\| :\|\|::\|\|@LB@ UCGUGA UUUUUAA@LB@3'-UUU...GUUCU AU GCAAU...CAA-5'@LB@ \| \|@LB@ 42 26@LB@@LB@@LB@Energy -7.21 kcal/mol@LB@Hybridization Energy -10.1429 kcal/mol@LB@Unfolding Energy - mRNA 1.29 kcal/mol@LB@Unfolding Energy - sRNA 1.6427 kcal/mol@LB@@LB@Position - mRNA 53 -- 65@LB@Position - sRNA 27 -- 41@LB@Position Seed - mRNA 59 -- 65@LB@Position Seed - sRNA 27 -- 33@LB@
28	0.0008326	0.09021	b2563	acpS	-7.40	0.086911	170 -- 193	37 -- 61	holo-[acyl-carrier-protein] synthase 1	_____________169_______________________194\n_____________\|_________________________\|\n5'-GCC...AGAAA______GU___GAU__U________GUGGC...GCG-3'\n______________UGAAGC___CU___GC_GGAAGCGC\n______________\|\|\|\|:\|___\|\|___\|\|_:\|\|\|\|\|:\|\n______________ACUUUG___GA___CG_UCUUCGUG\n3'-UUU...GCCCU______AAC__AUU__U________AAUUU...CAA-5'\n_____________\|_________________________\|\n_____________62________________________36\n	187 -- 193	37 -- 43	-21.5547 kcal/mol	6.80255 kcal/mol	7.3495 kcal/mol	169 194@LB@ \| \|@LB@5'-GCC...AGAAA GU GAU U GUGGC...GCG-3'@LB@ UGAAGC CU GC GGAAGCGC@LB@ \|\|\|\|:\| \|\| \|\| :\|\|\|\|\|:\|@LB@ ACUUUG GA CG UCUUCGUG@LB@3'-UUU...GCCCU AAC AUU U AAUUU...CAA-5'@LB@ \| \|@LB@ 62 36@LB@@LB@@LB@Energy -7.40 kcal/mol@LB@Hybridization Energy -21.5547 kcal/mol@LB@Unfolding Energy - mRNA 6.80255 kcal/mol@LB@Unfolding Energy - sRNA 7.3495 kcal/mol@LB@@LB@Position - mRNA 170 -- 193@LB@Position - sRNA 37 -- 61@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 37 -- 43@LB@
36	0.001384	0.121	b2573	rpoE	-5.96	0.197133	163 -- 178	25 -- 41	RNA polymerase sigma E factor	_____________162_______________179\n_____________\|_________________\|\n5'-ACA...UCGAU_____GU__________GUUUG...UGG-3'\n______________AGCGC___GGAAAUUUG\n______________\|\|\|:\|___::\|\|\|\|\|:\|\n______________UCGUG___UUUUUAAGC\n3'-UUU...GUUCU_____AAU_________AAUGU...CAA-5'\n_____________\|_________________\|\n_____________42________________24\n	172 -- 178	25 -- 31	-12.5895 kcal/mol	4.97735 kcal/mol	1.64839 kcal/mol	162 179@LB@ \| \|@LB@5'-ACA...UCGAU GU GUUUG...UGG-3'@LB@ AGCGC GGAAAUUUG@LB@ \|\|\|:\| ::\|\|\|\|\|:\|@LB@ UCGUG UUUUUAAGC@LB@3'-UUU...GUUCU AAU AAUGU...CAA-5'@LB@ \| \|@LB@ 42 24@LB@@LB@@LB@Energy -5.96 kcal/mol@LB@Hybridization Energy -12.5895 kcal/mol@LB@Unfolding Energy - mRNA 4.97735 kcal/mol@LB@Unfolding Energy - sRNA 1.64839 kcal/mol@LB@@LB@Position - mRNA 163 -- 178@LB@Position - sRNA 25 -- 41@LB@Position Seed - mRNA 172 -- 178@LB@Position Seed - sRNA 25 -- 31@LB@
154	0.01606	0.3359	b2587	kgtP	-4.55	0.394910	6 -- 12	52 -- 58	alpha-ketoglutarate transporter	_____________5_______13\n_____________\|_______\|\n______5'-UCCCC_______CCACU...UCG-3'\n______________AACUUGC\n______________\|\|\|\|\|\|\|\n______________UUGAACG\n3'-UUU...CUACU_______AAUUC...CAA-5'\n_____________\|_______\|\n_____________59______51\n	6 -- 12	52 -- 58	-7.82918 kcal/mol	0.15004 kcal/mol	3.13411 kcal/mol	5 13@LB@ \| \|@LB@ 5'-UCCCC CCACU...UCG-3'@LB@ AACUUGC@LB@ \|\|\|\|\|\|\|@LB@ UUGAACG@LB@3'-UUU...CUACU AAUUC...CAA-5'@LB@ \| \|@LB@ 59 51@LB@@LB@@LB@Energy -4.55 kcal/mol@LB@Hybridization Energy -7.82918 kcal/mol@LB@Unfolding Energy - mRNA 0.15004 kcal/mol@LB@Unfolding Energy - sRNA 3.13411 kcal/mol@LB@@LB@Position - mRNA 6 -- 12@LB@Position - sRNA 52 -- 58@LB@Position Seed - mRNA 6 -- 12@LB@Position Seed - sRNA 52 -- 58@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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