| 131 |
0.01151 |
0.2883 |
bsu03110 |
ycgH |
-12.02 |
0.104581 |
161 -- 192 |
27 -- 50 |
transporter |
|
_____________160______________________________193\n_____________|________________________________|\n5'-UUU...GGACA____ACA____AACCUUA____AA________AAUAA...UCG-3'\n______________GAUC___AUCU_______GAUA__AGGGGUUG\n______________||||___||||_______||||__||||||||\n______________CUAG___UAGA_______CUAU__UCCCCAAC\n3'-UUU...AAAGC____AAC_______________C_________CCCUU...AUA-5'\n_____________|________________________________|\n_____________51_______________________________26\n |
186 -- 192 |
27 -- 33 |
-20.341 kcal/mol |
7.34553 kcal/mol |
0.975523 kcal/mol |
160 193@LB@ | |@LB@5'-UUU...GGACA ACA AACCUUA AA AAUAA...UCG-3'@LB@ GAUC AUCU GAUA AGGGGUUG@LB@ |||| |||| |||| ||||||||@LB@ CUAG UAGA CUAU UCCCCAAC@LB@3'-UUU...AAAGC AAC C CCCUU...AUA-5'@LB@ | |@LB@ 51 26@LB@@LB@@LB@Energy -12.02 kcal/mol@LB@Hybridization Energy -20.341 kcal/mol@LB@Unfolding Energy - mRNA 7.34553 kcal/mol@LB@Unfolding Energy - sRNA 0.975523 kcal/mol@LB@@LB@Position - mRNA 161 -- 192@LB@Position - sRNA 27 -- 50@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 27 -- 33@LB@ |
| 132 |
0.01159 |
0.2883 |
bsu33140 |
yvqJ |
-14.19 |
0.043338 |
185 -- 204 |
31 -- 52 |
MFS transporter |
|
_____________184____________________205\n_____________|______________________|\n5'-GAC...GGCAA____GG___C_________U__UGAAU...UUA-3'\n______________CGGG____G_UCUGAUGGA_GG\n______________|||:____|_||||||:||_||\n______________GCCU____C_AGACUAUCU_CC\n3'-UUU...AAAAA____AGAA_U_________C__CAACC...AUA-5'\n_____________|______________________|\n_____________53_____________________30\n |
195 -- 201 |
34 -- 40 |
-19.4408 kcal/mol |
4.27934 kcal/mol |
0.976414 kcal/mol |
184 205@LB@ | |@LB@5'-GAC...GGCAA GG C U UGAAU...UUA-3'@LB@ CGGG G UCUGAUGGA GG@LB@ |||: | ||||||:|| ||@LB@ GCCU C AGACUAUCU CC@LB@3'-UUU...AAAAA AGAA U C CAACC...AUA-5'@LB@ | |@LB@ 53 30@LB@@LB@@LB@Energy -14.19 kcal/mol@LB@Hybridization Energy -19.4408 kcal/mol@LB@Unfolding Energy - mRNA 4.27934 kcal/mol@LB@Unfolding Energy - sRNA 0.976414 kcal/mol@LB@@LB@Position - mRNA 185 -- 204@LB@Position - sRNA 31 -- 52@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 133 |
0.01175 |
0.2883 |
bsu11610 |
ppnK |
-12.45 |
0.088788 |
273 -- 294 |
32 -- 53 |
inorganic polyphosphate/ATP-NAD kinase 1 |
|
_____________272____________________295\n_____________|______________________|\n5'-CCA...AUUUA____________A___AACU__AUGAAA-3'\n______________UUGGAUUUUGAU_UGG____GG\n______________|:||||:|||||_||:____||\n______________AGCCUAGAACUA_ACU____CC\n3'-UUU...AAAAA____________G___AUCU__CCAAC...AUA-5'\n_____________|______________________|\n_____________54_____________________31\n |
278 -- 284 |
42 -- 48 |
-17.5759 kcal/mol |
4.14878 kcal/mol |
0.981755 kcal/mol |
272 295@LB@ | |@LB@5'-CCA...AUUUA A AACU AUGAAA-3'@LB@ UUGGAUUUUGAU UGG GG@LB@ |:||||:||||| ||: ||@LB@ AGCCUAGAACUA ACU CC@LB@3'-UUU...AAAAA G AUCU CCAAC...AUA-5'@LB@ | |@LB@ 54 31@LB@@LB@@LB@Energy -12.45 kcal/mol@LB@Hybridization Energy -17.5759 kcal/mol@LB@Unfolding Energy - mRNA 4.14878 kcal/mol@LB@Unfolding Energy - sRNA 0.981755 kcal/mol@LB@@LB@Position - mRNA 273 -- 294@LB@Position - sRNA 32 -- 53@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 134 |
0.01184 |
0.2883 |
bsu01450 |
cbiO |
-8.85 |
0.304208 |
221 -- 231 |
32 -- 43 |
energy-coupling factor transporter ATP-binding protein EcfA1 |
|
_____________220__________232\n_____________|____________|\n5'-AUA...UUGAU____________AUAUC...AUG-3'\n______________AUC_GGUAGAGG\n______________|||_|:||||||\n______________UAG_CUAUCUCC\n3'-UUU...AGAAC___A________CCAAC...AUA-5'\n_____________|____________|\n_____________44___________31\n |
225 -- 231 |
32 -- 38 |
-12.7681 kcal/mol |
2.95776 kcal/mol |
0.959872 kcal/mol |
220 232@LB@ | |@LB@5'-AUA...UUGAU AUAUC...AUG-3'@LB@ AUC GGUAGAGG@LB@ ||| |:||||||@LB@ UAG CUAUCUCC@LB@3'-UUU...AGAAC A CCAAC...AUA-5'@LB@ | |@LB@ 44 31@LB@@LB@@LB@Energy -8.85 kcal/mol@LB@Hybridization Energy -12.7681 kcal/mol@LB@Unfolding Energy - mRNA 2.95776 kcal/mol@LB@Unfolding Energy - sRNA 0.959872 kcal/mol@LB@@LB@Position - mRNA 221 -- 231@LB@Position - sRNA 32 -- 43@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 135 |
0.01203 |
0.2883 |
bsu09920 |
yhaN |
-10.31 |
0.192319 |
8 -- 29 |
28 -- 47 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 16267290; Product type pe : enzyme' |
|
_____________7______________________30\n_____________|______________________|\n____5'-AACAAAC_______CUUUUUUU__C____UAUUU...ACG-3'\n______________CUUGAUC________GG_GGUU\n______________|||||||________||_||||\n______________GAACUAG________CC_CCAA\n3'-UUU...GCCUA_______ACUAUCU________CCCCU...AUA-5'\n_____________|______________________|\n_____________48_____________________27\n |
8 -- 14 |
41 -- 47 |
-14.5368 kcal/mol |
3.24797 kcal/mol |
0.974663 kcal/mol |
7 30@LB@ | |@LB@ 5'-AACAAAC CUUUUUUU C UAUUU...ACG-3'@LB@ CUUGAUC GG GGUU@LB@ ||||||| || ||||@LB@ GAACUAG CC CCAA@LB@3'-UUU...GCCUA ACUAUCU CCCCU...AUA-5'@LB@ | |@LB@ 48 27@LB@@LB@@LB@Energy -10.31 kcal/mol@LB@Hybridization Energy -14.5368 kcal/mol@LB@Unfolding Energy - mRNA 3.24797 kcal/mol@LB@Unfolding Energy - sRNA 0.974663 kcal/mol@LB@@LB@Position - mRNA 8 -- 29@LB@Position - sRNA 28 -- 47@LB@Position Seed - mRNA 8 -- 14@LB@Position Seed - sRNA 41 -- 47@LB@ |
| 136 |
0.01205 |
0.2883 |
bsu35319 |
yvzG |
-14.21 |
0.042782 |
258 -- 272 |
27 -- 43 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________257_______________273\n_____________|_________________|\n5'-AAG...UUACC_________________UGUUA...AUU-3'\n______________AUC_GA_AGAGGGGUUG\n______________|||_||_||||||||||\n______________UAG_CU_UCUCCCCAAC\n3'-UUU...AGAAC___A__A__________CCCUU...AUA-5'\n_____________|_________________|\n_____________44________________26\n |
266 -- 272 |
27 -- 33 |
-20.1473 kcal/mol |
4.96591 kcal/mol |
0.966729 kcal/mol |
257 273@LB@ | |@LB@5'-AAG...UUACC UGUUA...AUU-3'@LB@ AUC GA AGAGGGGUUG@LB@ ||| || ||||||||||@LB@ UAG CU UCUCCCCAAC@LB@3'-UUU...AGAAC A A CCCUU...AUA-5'@LB@ | |@LB@ 44 26@LB@@LB@@LB@Energy -14.21 kcal/mol@LB@Hybridization Energy -20.1473 kcal/mol@LB@Unfolding Energy - mRNA 4.96591 kcal/mol@LB@Unfolding Energy - sRNA 0.966729 kcal/mol@LB@@LB@Position - mRNA 258 -- 272@LB@Position - sRNA 27 -- 43@LB@Position Seed - mRNA 266 -- 272@LB@Position Seed - sRNA 27 -- 33@LB@ |
| 137 |
0.01211 |
0.2883 |
bsu27850 |
nadA |
-8.79 |
0.309443 |
168 -- 191 |
32 -- 52 |
quinolinate synthase A |
|
_____________167_______________________192\n_____________|_________________________|\n5'-UAC...UGUUA__UU___AAA__A____________AAUGC...UUA-3'\n______________CG__AUC___UG_AUCU_GUGGAGG\n______________||__|||___||_||||_:|:||||\n______________GC__UAG___AC_UAGA_UAUCUCC\n3'-UUU...AAAAA__C____A_________C_______CCAAC...AUA-5'\n_____________|_________________________|\n_____________53________________________31\n |
185 -- 191 |
32 -- 38 |
-13.8724 kcal/mol |
4.10431 kcal/mol |
0.976384 kcal/mol |
167 192@LB@ | |@LB@5'-UAC...UGUUA UU AAA A AAUGC...UUA-3'@LB@ CG AUC UG AUCU GUGGAGG@LB@ || ||| || |||| :|:||||@LB@ GC UAG AC UAGA UAUCUCC@LB@3'-UUU...AAAAA C A C CCAAC...AUA-5'@LB@ | |@LB@ 53 31@LB@@LB@@LB@Energy -8.79 kcal/mol@LB@Hybridization Energy -13.8724 kcal/mol@LB@Unfolding Energy - mRNA 4.10431 kcal/mol@LB@Unfolding Energy - sRNA 0.976384 kcal/mol@LB@@LB@Position - mRNA 168 -- 191@LB@Position - sRNA 32 -- 52@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 138 |
0.01215 |
0.2883 |
bsu17940 |
yneI |
-15.06 |
0.029368 |
176 -- 197 |
30 -- 52 |
plasmid maintenance protein CcdB |
|
_____________175_____________________198\n_____________|_______________________|\n5'-GAA...AUCUG____AA_C____GU_________AAUAU...CGG-3'\n______________UGGA__U_GAUU___UGGAGGGG\n______________:|||__|_|||:___|:||||||\n______________GCCU__A_CUAG___AUCUCCCC\n3'-UUU...AAAAA____AG_A____ACU________AACCC...AUA-5'\n_____________|_______________________|\n_____________53______________________29\n |
191 -- 197 |
30 -- 36 |
-21.3361 kcal/mol |
5.29839 kcal/mol |
0.979974 kcal/mol |
175 198@LB@ | |@LB@5'-GAA...AUCUG AA C GU AAUAU...CGG-3'@LB@ UGGA U GAUU UGGAGGGG@LB@ :||| | |||: |:||||||@LB@ GCCU A CUAG AUCUCCCC@LB@3'-UUU...AAAAA AG A ACU AACCC...AUA-5'@LB@ | |@LB@ 53 29@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -21.3361 kcal/mol@LB@Unfolding Energy - mRNA 5.29839 kcal/mol@LB@Unfolding Energy - sRNA 0.979974 kcal/mol@LB@@LB@Position - mRNA 176 -- 197@LB@Position - sRNA 30 -- 52@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 139 |
0.01217 |
0.2883 |
bsu30960 |
glgD |
-11.28 |
0.137516 |
171 -- 191 |
30 -- 50 |
glycogen biosynthesis protein GlgD |
|
_____________170____________________192\n_____________|______________________|\n5'-ACU...GAAUU_____AAUUA____________GCAGA...UUG-3'\n______________GAUUU_____CUGA_AGAGGGG\n______________|||:|_____||||_|||||||\n______________CUAGA_____GACU_UCUCCCC\n3'-UUU...AAAGC_____ACUA_____A_______AACCC...AUA-5'\n_____________|______________________|\n_____________51_____________________29\n |
185 -- 191 |
30 -- 36 |
-19.1028 kcal/mol |
6.84884 kcal/mol |
0.974272 kcal/mol |
170 192@LB@ | |@LB@5'-ACU...GAAUU AAUUA GCAGA...UUG-3'@LB@ GAUUU CUGA AGAGGGG@LB@ |||:| |||| |||||||@LB@ CUAGA GACU UCUCCCC@LB@3'-UUU...AAAGC ACUA A AACCC...AUA-5'@LB@ | |@LB@ 51 29@LB@@LB@@LB@Energy -11.28 kcal/mol@LB@Hybridization Energy -19.1028 kcal/mol@LB@Unfolding Energy - mRNA 6.84884 kcal/mol@LB@Unfolding Energy - sRNA 0.974272 kcal/mol@LB@@LB@Position - mRNA 171 -- 191@LB@Position - sRNA 30 -- 50@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 140 |
0.01224 |
0.2883 |
bsu36480 |
ywoD |
-6.33 |
0.569704 |
31 -- 37 |
66 -- 72 |
MFS transporter |
|
_____________30______38\n_____________|_______|\n5'-AUU...AUAUC_______AAAGC...AUA-3'\n______________GGGGUGG\n______________|||||:|\n______________CCCCAUC\n3'-UUU...ACCGC_______GCGGU...AUA-5'\n_____________|_______|\n_____________73______65\n |
31 -- 37 |
66 -- 72 |
-11.1749 kcal/mol |
4.83937 kcal/mol |
0.00366414 kcal/mol |
30 38@LB@ | |@LB@5'-AUU...AUAUC AAAGC...AUA-3'@LB@ GGGGUGG@LB@ |||||:|@LB@ CCCCAUC@LB@3'-UUU...ACCGC GCGGU...AUA-5'@LB@ | |@LB@ 73 65@LB@@LB@@LB@Energy -6.33 kcal/mol@LB@Hybridization Energy -11.1749 kcal/mol@LB@Unfolding Energy - mRNA 4.83937 kcal/mol@LB@Unfolding Energy - sRNA 0.00366414 kcal/mol@LB@@LB@Position - mRNA 31 -- 37@LB@Position - sRNA 66 -- 72@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 66 -- 72@LB@ |
