| 11 |
0.0001235 |
0.03747 |
bsu15610 |
sumT |
-12.35 |
0.092299 |
189 -- 208 |
22 -- 34 |
uroporphyrinogen-iii C-methyltransferase |
|
_____________188__________________209\n_____________|____________________|\n5'-GAA...AGAUC_____AAAUACGA_______AGUAU...AUG-3'\n______________GGGGG________UGGGGAA\n______________:||||________|||||||\n______________UCCCC________ACCCCUU\n3'-UUU...CUAUC_____A______________GUCUC...AUA-5'\n_____________|____________________|\n_____________35___________________21\n |
202 -- 208 |
22 -- 28 |
-16.2617 kcal/mol |
2.89451 kcal/mol |
1.02154 kcal/mol |
188 209@LB@ | |@LB@5'-GAA...AGAUC AAAUACGA AGUAU...AUG-3'@LB@ GGGGG UGGGGAA@LB@ :|||| |||||||@LB@ UCCCC ACCCCUU@LB@3'-UUU...CUAUC A GUCUC...AUA-5'@LB@ | |@LB@ 35 21@LB@@LB@@LB@Energy -12.35 kcal/mol@LB@Hybridization Energy -16.2617 kcal/mol@LB@Unfolding Energy - mRNA 2.89451 kcal/mol@LB@Unfolding Energy - sRNA 1.02154 kcal/mol@LB@@LB@Position - mRNA 189 -- 208@LB@Position - sRNA 22 -- 34@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 22 -- 28@LB@ |
| 12 |
0.0001633 |
0.0454 |
bsu11410 |
yjbA |
-19.25 |
0.003321 |
222 -- 243 |
30 -- 52 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type pf: factor' |
|
_____________221_______________________244\n_____________|_________________________|\n5'-GAA...AUGUG______AA_________________AAGAA...UUG-3'\n______________UGGGUC__UUGGUUUGA___AGGGG\n______________:||:||__|||:|:|||___|||||\n______________GCCUAG__AACUAGACU___UCCCC\n3'-UUU...AAAAA_________________AUC_____AACCC...AUA-5'\n_____________|_________________________|\n_____________53________________________29\n |
232 -- 238 |
38 -- 44 |
-23.9108 kcal/mol |
3.6845 kcal/mol |
0.979974 kcal/mol |
221 244@LB@ | |@LB@5'-GAA...AUGUG AA AAGAA...UUG-3'@LB@ UGGGUC UUGGUUUGA AGGGG@LB@ :||:|| |||:|:||| |||||@LB@ GCCUAG AACUAGACU UCCCC@LB@3'-UUU...AAAAA AUC AACCC...AUA-5'@LB@ | |@LB@ 53 29@LB@@LB@@LB@Energy -19.25 kcal/mol@LB@Hybridization Energy -23.9108 kcal/mol@LB@Unfolding Energy - mRNA 3.6845 kcal/mol@LB@Unfolding Energy - sRNA 0.979974 kcal/mol@LB@@LB@Position - mRNA 222 -- 243@LB@Position - sRNA 30 -- 52@LB@Position Seed - mRNA 232 -- 238@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 13 |
0.0002088 |
0.05257 |
bsu22150 |
ypvA |
-19.04 |
0.003759 |
161 -- 194 |
26 -- 53 |
ATP-dependent helicase YpvA |
|
_____________160____________________________________195\n_____________|______________________________________|\n5'-GUG...UAUUA________________AUGUCGAA___UU_________AACUA...CCG-3'\n______________UUGG____UGGUCUGA________UAG__GGGGGUUGG\n______________|:||____||:|||||________|||__:||||||||\n______________AGCC____ACUAGACU________AUC__UCCCCAACC\n3'-UUU...AAAAA____UAGA______________________________CCUUG...AUA-5'\n_____________|______________________________________|\n_____________54_____________________________________25\n |
188 -- 194 |
26 -- 32 |
-27.605 kcal/mol |
7.58324 kcal/mol |
0.986187 kcal/mol |
160 195@LB@ | |@LB@5'-GUG...UAUUA AUGUCGAA UU AACUA...CCG-3'@LB@ UUGG UGGUCUGA UAG GGGGGUUGG@LB@ |:|| ||:||||| ||| :||||||||@LB@ AGCC ACUAGACU AUC UCCCCAACC@LB@3'-UUU...AAAAA UAGA CCUUG...AUA-5'@LB@ | |@LB@ 54 25@LB@@LB@@LB@Energy -19.04 kcal/mol@LB@Hybridization Energy -27.605 kcal/mol@LB@Unfolding Energy - mRNA 7.58324 kcal/mol@LB@Unfolding Energy - sRNA 0.986187 kcal/mol@LB@@LB@Position - mRNA 161 -- 194@LB@Position - sRNA 26 -- 53@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 26 -- 32@LB@ |
| 14 |
0.0002247 |
0.05257 |
bsu30160 |
ytcQ |
-17.33 |
0.009670 |
159 -- 223 |
10 -- 51 |
peptide ABC transporter substrate-binding protein |
|
_____________158__________________________________________________________________224\n_____________|____________________________________________________________________|\n5'-UUG...UGCUG___________AAAGGAUAAAGAAAUA___AAC________ACGA____A___AAAU___________UUUGA...GCA-3'\n______________GGAUCU__GUC________________UGA___GGAGGGGU____UGGG_AAC____GGAGGGU_GCU\n______________||||||__:||________________|||___:|||||||____||||_|||____:|||:||_|||\n______________CCUAGA__UAG________________ACU___UCUCCCCA____ACCC_UUG____UCUCUCA_CGA\n3'-UUU...AAAAG______AC______________________A__________________C______________U___AGAGA...AUA-5'\n_____________|____________________________________________________________________|\n_____________52___________________________________________________________________9\n |
214 -- 220 |
14 -- 20 |
-40.5 kcal/mol |
13.2168 kcal/mol |
9.95655 kcal/mol |
158 224@LB@ | |@LB@5'-UUG...UGCUG AAAGGAUAAAGAAAUA AAC ACGA A AAAU UUUGA...GCA-3'@LB@ GGAUCU GUC UGA GGAGGGGU UGGG AAC GGAGGGU GCU@LB@ |||||| :|| ||| :||||||| |||| ||| :|||:|| |||@LB@ CCUAGA UAG ACU UCUCCCCA ACCC UUG UCUCUCA CGA@LB@3'-UUU...AAAAG AC A C U AGAGA...AUA-5'@LB@ | |@LB@ 52 9@LB@@LB@@LB@Energy -17.33 kcal/mol@LB@Hybridization Energy -40.5 kcal/mol@LB@Unfolding Energy - mRNA 13.2168 kcal/mol@LB@Unfolding Energy - sRNA 9.95655 kcal/mol@LB@@LB@Position - mRNA 159 -- 223@LB@Position - sRNA 10 -- 51@LB@Position Seed - mRNA 214 -- 220@LB@Position Seed - sRNA 14 -- 20@LB@ |
| 15 |
0.0002364 |
0.05257 |
bsu19290 |
yozO |
-13.57 |
0.056229 |
184 -- 195 |
28 -- 39 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________183__________196\n_____________|____________|\n5'-AAA...AUGCA__A_________CUUCA...UGG-3'\n______________GA_AGAGGGGUU\n______________||_|||||||||\n______________CU_UCUCCCCAA\n3'-UUU...CUAGA__A_________CCCCU...AUA-5'\n_____________|____________|\n_____________40___________27\n |
189 -- 195 |
28 -- 34 |
-16.9963 kcal/mol |
2.64187 kcal/mol |
0.77983 kcal/mol |
183 196@LB@ | |@LB@5'-AAA...AUGCA A CUUCA...UGG-3'@LB@ GA AGAGGGGUU@LB@ || |||||||||@LB@ CU UCUCCCCAA@LB@3'-UUU...CUAGA A CCCCU...AUA-5'@LB@ | |@LB@ 40 27@LB@@LB@@LB@Energy -13.57 kcal/mol@LB@Hybridization Energy -16.9963 kcal/mol@LB@Unfolding Energy - mRNA 2.64187 kcal/mol@LB@Unfolding Energy - sRNA 0.77983 kcal/mol@LB@@LB@Position - mRNA 184 -- 195@LB@Position - sRNA 28 -- 39@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 16 |
0.0002979 |
0.06212 |
bsu28440 |
sdhA |
-14.86 |
0.032148 |
176 -- 195 |
24 -- 44 |
succinate dehydrogenase flavoprotein subunit |
|
_____________175___________________196\n_____________|_____________________|\n5'-GUG...UACUA____AC________AGA____CUAUC...CCA-3'\n______________GAUU___UAGAGGG___GGGG\n______________|||:___|||||||___||||\n______________CUAG___AUCUCCC___CCCC\n3'-UUU...UAGAA____ACU_______CAA____UUGUC...AUA-5'\n_____________|_____________________|\n_____________45____________________23\n |
182 -- 188 |
31 -- 37 |
-20.465 kcal/mol |
4.62868 kcal/mol |
0.977523 kcal/mol |
175 196@LB@ | |@LB@5'-GUG...UACUA AC AGA CUAUC...CCA-3'@LB@ GAUU UAGAGGG GGGG@LB@ |||: ||||||| ||||@LB@ CUAG AUCUCCC CCCC@LB@3'-UUU...UAGAA ACU CAA UUGUC...AUA-5'@LB@ | |@LB@ 45 23@LB@@LB@@LB@Energy -14.86 kcal/mol@LB@Hybridization Energy -20.465 kcal/mol@LB@Unfolding Energy - mRNA 4.62868 kcal/mol@LB@Unfolding Energy - sRNA 0.977523 kcal/mol@LB@@LB@Position - mRNA 176 -- 195@LB@Position - sRNA 24 -- 44@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 17 |
0.0003284 |
0.06216 |
bsu08690 |
ygaD |
-15.59 |
0.022949 |
198 -- 218 |
30 -- 49 |
ABC transporter ATP-binding protein |
|
_____________197___________________219\n_____________|_____________________|\n5'-UGA...CACAC___A____AAA__G_______GAAUC...UCG-3'\n______________AUC_UGAU___GA_AGAGGGG\n______________|||_||||___||_|||||||\n______________UAG_ACUA___CU_UCUCCCC\n3'-UUU...AAGCC___A____GA___A_______AACCC...AUA-5'\n_____________|_____________________|\n_____________50____________________29\n |
212 -- 218 |
30 -- 36 |
-19.3788 kcal/mol |
2.8161 kcal/mol |
0.974265 kcal/mol |
197 219@LB@ | |@LB@5'-UGA...CACAC A AAA G GAAUC...UCG-3'@LB@ AUC UGAU GA AGAGGGG@LB@ ||| |||| || |||||||@LB@ UAG ACUA CU UCUCCCC@LB@3'-UUU...AAGCC A GA A AACCC...AUA-5'@LB@ | |@LB@ 50 29@LB@@LB@@LB@Energy -15.59 kcal/mol@LB@Hybridization Energy -19.3788 kcal/mol@LB@Unfolding Energy - mRNA 2.8161 kcal/mol@LB@Unfolding Energy - sRNA 0.974265 kcal/mol@LB@@LB@Position - mRNA 198 -- 218@LB@Position - sRNA 30 -- 49@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 18 |
0.00036 |
0.06216 |
bsu24510 |
yqhO |
-21.34 |
0.000873 |
175 -- 205 |
13 -- 44 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________174_______________________________206\n_____________|_________________________________|\n5'-UAU...UAAAC____G__________GA____________U___UAUUG...GAG-3'\n______________GAUC_GAUGGAGGGG___GGGAAU_GAGA_GUA\n______________||||_|||:||||||___|||||:_||||_|||\n______________CUAG_CUAUCUCCCC___CCCUUG_CUCU_CAU\n3'-UUU...UAGAA____A__________AAC______U________CGAAG...AUA-5'\n_____________|_________________________________|\n_____________45________________________________12\n |
183 -- 189 |
30 -- 36 |
-39.4543 kcal/mol |
8.17177 kcal/mol |
9.94171 kcal/mol |
174 206@LB@ | |@LB@5'-UAU...UAAAC G GA U UAUUG...GAG-3'@LB@ GAUC GAUGGAGGGG GGGAAU GAGA GUA@LB@ |||| |||:|||||| |||||: |||| |||@LB@ CUAG CUAUCUCCCC CCCUUG CUCU CAU@LB@3'-UUU...UAGAA A AAC U CGAAG...AUA-5'@LB@ | |@LB@ 45 12@LB@@LB@@LB@Energy -21.34 kcal/mol@LB@Hybridization Energy -39.4543 kcal/mol@LB@Unfolding Energy - mRNA 8.17177 kcal/mol@LB@Unfolding Energy - sRNA 9.94171 kcal/mol@LB@@LB@Position - mRNA 175 -- 205@LB@Position - sRNA 13 -- 44@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 19 |
0.0003652 |
0.06216 |
bsu29920 |
ytmP |
-16.30 |
0.016289 |
3 -- 31 |
25 -- 51 |
phosphotransferase YtmP |
|
_____________2_____________________________32\n_____________|_____________________________|\n_________5'-AU_____GAACA______U__ACU_______ACAAU...GUA-3'\n______________GGGUU_____UUGAUA_GA___GGUUGGG\n______________||:|:_____:|||||_||___|||||||\n______________CCUAG_____GACUAU_CU___CCAACCC\n3'-UUU...AAAAG_____AACUA_________CC________CUUGU...AUA-5'\n_____________|_____________________________|\n_____________52____________________________24\n |
25 -- 31 |
25 -- 31 |
-23.4751 kcal/mol |
6.19942 kcal/mol |
0.976067 kcal/mol |
2 32@LB@ | |@LB@ 5'-AU GAACA U ACU ACAAU...GUA-3'@LB@ GGGUU UUGAUA GA GGUUGGG@LB@ ||:|: :||||| || |||||||@LB@ CCUAG GACUAU CU CCAACCC@LB@3'-UUU...AAAAG AACUA CC CUUGU...AUA-5'@LB@ | |@LB@ 52 24@LB@@LB@@LB@Energy -16.30 kcal/mol@LB@Hybridization Energy -23.4751 kcal/mol@LB@Unfolding Energy - mRNA 6.19942 kcal/mol@LB@Unfolding Energy - sRNA 0.976067 kcal/mol@LB@@LB@Position - mRNA 3 -- 31@LB@Position - sRNA 25 -- 51@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 25 -- 31@LB@ |
| 20 |
0.0003727 |
0.06216 |
bsu04690 |
rsbT |
-12.89 |
0.074349 |
161 -- 193 |
22 -- 51 |
serine/threonine protein kinase |
|
_____________160________________________________194\n_____________|__________________________________|\n5'-UUG...GAGCA__GG_____GACAU______C___AA________AUAGC...CGG-3'\n______________GG__CUUGA_____UGA_AG_GGG__UUGGGGGA\n______________||__|||||_____|||_||_|||__||||||:|\n______________CC__GAACU_____ACU_UC_CCC__AACCCCUU\n3'-UUU...AAAAG__UA_____AG______A__U___C_________GUCUC...AUA-5'\n_____________|__________________________________|\n_____________52_________________________________21\n |
187 -- 193 |
22 -- 28 |
-26.679 kcal/mol |
11.9064 kcal/mol |
1.87888 kcal/mol |
160 194@LB@ | |@LB@5'-UUG...GAGCA GG GACAU C AA AUAGC...CGG-3'@LB@ GG CUUGA UGA AG GGG UUGGGGGA@LB@ || ||||| ||| || ||| ||||||:|@LB@ CC GAACU ACU UC CCC AACCCCUU@LB@3'-UUU...AAAAG UA AG A U C GUCUC...AUA-5'@LB@ | |@LB@ 52 21@LB@@LB@@LB@Energy -12.89 kcal/mol@LB@Hybridization Energy -26.679 kcal/mol@LB@Unfolding Energy - mRNA 11.9064 kcal/mol@LB@Unfolding Energy - sRNA 1.87888 kcal/mol@LB@@LB@Position - mRNA 161 -- 193@LB@Position - sRNA 22 -- 51@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 22 -- 28@LB@ |
