Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
83	0.001985	0.08414	b1901	araF	-10.36	0.035498	164 -- 171	1 -- 8	L-arabinose ABC transporter periplasmic binding protein		_____________163______172\n_____________\|________\|\n5'-UCC...GUUUU________ACAAA...AGC-3'\n______________UGCCCUAC\n______________\|:\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	165 -- 171	1 -- 7	-13.292 kcal/mol	0.840759 kcal/mol	2.11603 kcal/mol	163 172@LB@ \| \|@LB@5'-UCC...GUUUU ACAAA...AGC-3'@LB@ UGCCCUAC@LB@ \|:\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -10.36 kcal/mol@LB@Hybridization Energy -13.292 kcal/mol@LB@Unfolding Energy - mRNA 0.840759 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 164 -- 171@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 1 -- 7@LB@
119	0.003968	0.1172	b2765	queD	-8.07	0.149030	164 -- 172	1 -- 9	6-pyruvoyl tetrahydrobiopterin synthase (PTPS)		_____________163_______173\n_____________\|_________\|\n5'-AAC...UUCCG_________GGCGG...AUU-3'\n______________GUACUCUAC\n______________\|\|\|\|:\|\|\|\|\n______________CAUGGGAUG\n3'-AUU...GGAGA_________-5'\n_____________\|________\|\n_____________10_______1\n	166 -- 172	1 -- 7	-12.1088 kcal/mol	1.92954 kcal/mol	2.12872 kcal/mol	163 173@LB@ \| \|@LB@5'-AAC...UUCCG GGCGG...AUU-3'@LB@ GUACUCUAC@LB@ \|\|\|\|:\|\|\|\|@LB@ CAUGGGAUG@LB@3'-AUU...GGAGA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -8.07 kcal/mol@LB@Hybridization Energy -12.1088 kcal/mol@LB@Unfolding Energy - mRNA 1.92954 kcal/mol@LB@Unfolding Energy - sRNA 2.12872 kcal/mol@LB@@LB@Position - mRNA 164 -- 172@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 1 -- 7@LB@
182	0.007896	0.1519	b0686	ybfF	-7.34	0.221050	164 -- 170	2 -- 8	acyl-CoA esterase		_____________163_____171\n_____________\|_______\|\n5'-CUU...CAUAA_______ACCCG...GCG-3'\n______________UACCCUA\n______________\|\|\|\|\|\|\|\n______________AUGGGAU\n3'-AUU...GAGAC_______G-5'\n_____________\|_______\|\n_____________9_______1\n	164 -- 170	2 -- 8	-9.7212 kcal/mol	0.292492 kcal/mol	2.11083 kcal/mol	163 171@LB@ \| \|@LB@5'-CUU...CAUAA ACCCG...GCG-3'@LB@ UACCCUA@LB@ \|\|\|\|\|\|\|@LB@ AUGGGAU@LB@3'-AUU...GAGAC G-5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -7.34 kcal/mol@LB@Hybridization Energy -9.7212 kcal/mol@LB@Unfolding Energy - mRNA 0.292492 kcal/mol@LB@Unfolding Energy - sRNA 2.11083 kcal/mol@LB@@LB@Position - mRNA 164 -- 170@LB@Position - sRNA 2 -- 8@LB@Position Seed - mRNA 164 -- 170@LB@Position Seed - sRNA 2 -- 8@LB@
11	3.674e-05	0.01134	b2099	yegU	-10.44	0.033453	166 -- 229	3 -- 59	ADP-ribosylglycohydrolase family protein		_____________165_________________________________________________________________230\n_____________\|___________________________________________________________________\|\n5'-GUG...UCGAU____G____AU____U___CAC______AAGUUAAA_____AC________AU______G_______UUAUG...CAC-3'\n______________GAUC_CGAU__UGCG_UGA___AAGGGG________UGAAA___AGAACGU____UCUC_GUGCUCU\n______________:\|\|\|_\|\|\|\|__\|:\|\|_\|\|\|___\|\|::\|\|________\|\|\|\|\|___\|\|\|\|\|:\|____:\|\|\|_\|\|:\|:\|\|\n______________UUAG_GCUA__AUGC_ACU___UUUUCC________ACUUU___UCUUGUA____GGAG_CAUGGGA\n3'-AUU...UCGGU___________________UCA______AG___________CUA_______GAAU____A_______UG-5'\n_____________\|___________________________________________________________________\|\n_____________60__________________________________________________________________2\n	223 -- 229	3 -- 9	-36.2809 kcal/mol	13.1575 kcal/mol	12.7026 kcal/mol	165 230@LB@ \| \|@LB@5'-GUG...UCGAU G AU U CAC AAGUUAAA AC AU G UUAUG...CAC-3'@LB@ GAUC CGAU UGCG UGA AAGGGG UGAAA AGAACGU UCUC GUGCUCU@LB@ :\|\|\| \|\|\|\| \|:\|\| \|\|\| \|\|::\|\| \|\|\|\|\| \|\|\|\|\|:\| :\|\|\| \|\|:\|:\|\|@LB@ UUAG GCUA AUGC ACU UUUUCC ACUUU UCUUGUA GGAG CAUGGGA@LB@3'-AUU...UCGGU UCA AG CUA GAAU A UG-5'@LB@ \| \|@LB@ 60 2@LB@@LB@@LB@Energy -10.44 kcal/mol@LB@Hybridization Energy -36.2809 kcal/mol@LB@Unfolding Energy - mRNA 13.1575 kcal/mol@LB@Unfolding Energy - sRNA 12.7026 kcal/mol@LB@@LB@Position - mRNA 166 -- 229@LB@Position - sRNA 3 -- 59@LB@Position Seed - mRNA 223 -- 229@LB@Position Seed - sRNA 3 -- 9@LB@
53	0.0007581	0.04961	b1302	puuE	-16.93	0.000076	166 -- 207	20 -- 57	4-aminobutyrate aminotransferase PLP-dependent	b2662	_____________165_________________________________________208\n_____________\|___________________________________________\|\n5'-AGC...UAAUU________AC_______AGUC_______G_____C________ACAAU...CGC-3'\n______________UCCGAUUA__CGUGAAG____AAAAGGU_UGAAA_AU_GAGCA\n______________\|\|\|\|\|\|\|\|__\|\|\|\|\|\|\|____\|\|\|\|\|\|\|_\|\|\|\|\|_\|\|_\|\|:\|\|\n______________AGGCUAAU__GCACUUC____UUUUCCA_ACUUU_UA_CUUGU\n3'-AUU...GGUUU_________________A__________G_____C__U_____AGAAU...AUG-5'\n_____________\|___________________________________________\|\n_____________58__________________________________________19\n	176 -- 182	43 -- 49	-36.956 kcal/mol	7.85943 kcal/mol	12.1551 kcal/mol	165 208@LB@ \| \|@LB@5'-AGC...UAAUU AC AGUC G C ACAAU...CGC-3'@LB@ UCCGAUUA CGUGAAG AAAAGGU UGAAA AU GAGCA@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\| \|\|:\|\|@LB@ AGGCUAAU GCACUUC UUUUCCA ACUUU UA CUUGU@LB@3'-AUU...GGUUU A G C U AGAAU...AUG-5'@LB@ \| \|@LB@ 58 19@LB@@LB@@LB@Energy -16.93 kcal/mol@LB@Hybridization Energy -36.956 kcal/mol@LB@Unfolding Energy - mRNA 7.85943 kcal/mol@LB@Unfolding Energy - sRNA 12.1551 kcal/mol@LB@@LB@Position - mRNA 166 -- 207@LB@Position - sRNA 20 -- 57@LB@Position Seed - mRNA 176 -- 182@LB@Position Seed - sRNA 43 -- 49@LB@
86	0.002239	0.09158	b0434	yajG	-10.16	0.040736	167 -- 223	4 -- 56	putative lipoprotein		_____________166____________________________________________________________224\n_____________\|______________________________________________________________\|\n5'-UGG...UUUUA__AAAC___CU_________UC________CCC______G__CAAAAAAAU_____U_____GUUAG...UGC-3'\n______________CC____ACG_____UAAGAG__UCUGAGAG___GAACAU_UU_________CCUCU___CCC\n______________\|\|____\|\|\|_____\|\|\|:\|\|__\|\|\|\|\|:\|\|___\|\|\|\|\|\|_\|\|_________\|\|\|\|\|___\|\|\|\n______________GG____UGC_____AUUUUC__AGACUUUC___CUUGUA_AA_________GGAGA___GGG\n3'-AUU...GUUUA__CUAA___ACUUC______C_________UAU______G__U_____________CAU___AUG-5'\n_____________\|______________________________________________________________\|\n_____________57_____________________________________________________________3\n	187 -- 193	28 -- 34	-29.9553 kcal/mol	7.1187 kcal/mol	12.7017 kcal/mol	166 224@LB@ \| \|@LB@5'-UGG...UUUUA AAAC CU UC CCC G CAAAAAAAU U GUUAG...UGC-3'@LB@ CC ACG UAAGAG UCUGAGAG GAACAU UU CCUCU CCC@LB@ \|\| \|\|\| \|\|\|:\|\| \|\|\|\|\|:\|\| \|\|\|\|\|\| \|\| \|\|\|\|\| \|\|\|@LB@ GG UGC AUUUUC AGACUUUC CUUGUA AA GGAGA GGG@LB@3'-AUU...GUUUA CUAA ACUUC C UAU G U CAU AUG-5'@LB@ \| \|@LB@ 57 3@LB@@LB@@LB@Energy -10.16 kcal/mol@LB@Hybridization Energy -29.9553 kcal/mol@LB@Unfolding Energy - mRNA 7.1187 kcal/mol@LB@Unfolding Energy - sRNA 12.7017 kcal/mol@LB@@LB@Position - mRNA 167 -- 223@LB@Position - sRNA 4 -- 56@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 28 -- 34@LB@
34	0.0003499	0.03519	b0333	prpC	-9.01	0.085770	169 -- 175	1 -- 7	2-methylcitrate synthase		_____________168_____176\n_____________\|_______\|\n5'-ACU...UCUCG_______AAAUG...GCA-3'\n______________ACCCUAC\n______________\|\|\|\|\|\|\|\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	169 -- 175	1 -- 7	-11.7504 kcal/mol	0.722985 kcal/mol	2.03949 kcal/mol	168 176@LB@ \| \|@LB@5'-ACU...UCUCG AAAUG...GCA-3'@LB@ ACCCUAC@LB@ \|\|\|\|\|\|\|@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -9.01 kcal/mol@LB@Hybridization Energy -11.7504 kcal/mol@LB@Unfolding Energy - mRNA 0.722985 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 169 -- 175@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 169 -- 175@LB@Position Seed - sRNA 1 -- 7@LB@
112	0.003594	0.1129	b3866	yihI	-3.87	0.795256	171 -- 194	53 -- 75	activator of Der GTPase		_____________170______________________195\n_____________\|________________________\|\n5'-GAA...CUAUA_____UAAAA________AGA___ACCAU...GUC-3'\n______________CUGAA_____CGGCUAAA___CGA\n______________\|\|:\|\|_____\|:\|\|:\|\|\|___\|\|\|\n______________GAUUU_____GUCGGUUU___GCU\n3'-AUU...CCGCC_____UAUAA________AG____AAUGC...AUG-5'\n_____________\|________________________\|\n_____________76_______________________52\n	181 -- 187	59 -- 65	-14.4152 kcal/mol	4.20142 kcal/mol	6.36326 kcal/mol	170 195@LB@ \| \|@LB@5'-GAA...CUAUA UAAAA AGA ACCAU...GUC-3'@LB@ CUGAA CGGCUAAA CGA@LB@ \|\|:\|\| \|:\|\|:\|\|\| \|\|\|@LB@ GAUUU GUCGGUUU GCU@LB@3'-AUU...CCGCC UAUAA AG AAUGC...AUG-5'@LB@ \| \|@LB@ 76 52@LB@@LB@@LB@Energy -3.87 kcal/mol@LB@Hybridization Energy -14.4152 kcal/mol@LB@Unfolding Energy - mRNA 4.20142 kcal/mol@LB@Unfolding Energy - sRNA 6.36326 kcal/mol@LB@@LB@Position - mRNA 171 -- 194@LB@Position - sRNA 53 -- 75@LB@Position Seed - mRNA 181 -- 187@LB@Position Seed - sRNA 59 -- 65@LB@
132	0.004657	0.1239	b1200	dhaK	-7.52	0.201505	172 -- 179	1 -- 8	dihydroxyacetone kinase PTS-dependent dihydroxyacetone-binding subunit		_____________171______180\n_____________\|________\|\n5'-UUC...AUCCA________CGUAA...UGU-3'\n______________UGCCCUAC\n______________\|:\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	173 -- 179	1 -- 7	-12.1513 kcal/mol	2.5392 kcal/mol	2.11603 kcal/mol	171 180@LB@ \| \|@LB@5'-UUC...AUCCA CGUAA...UGU-3'@LB@ UGCCCUAC@LB@ \|:\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -7.52 kcal/mol@LB@Hybridization Energy -12.1513 kcal/mol@LB@Unfolding Energy - mRNA 2.5392 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 172 -- 179@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 173 -- 179@LB@Position Seed - sRNA 1 -- 7@LB@
168	0.006917	0.1448	b3070	yqjH	-8.25	0.134865	172 -- 181	49 -- 58	putative siderophore interacting protein		_____________171________182\n_____________\|__________\|\n5'-ACU...GAUAU__________CAUCA...AGC-3'\n______________AUCUGAUUAC\n______________\|\|\|:\|\|\|\|\|\|\n______________UAGGCUAAUG\n3'-AUU...CGGUU__________CACUU...AUG-5'\n_____________\|__________\|\n_____________59_________48\n	175 -- 181	49 -- 55	-10.8722 kcal/mol	2.31397 kcal/mol	0.318367 kcal/mol	171 182@LB@ \| \|@LB@5'-ACU...GAUAU CAUCA...AGC-3'@LB@ AUCUGAUUAC@LB@ \|\|\|:\|\|\|\|\|\|@LB@ UAGGCUAAUG@LB@3'-AUU...CGGUU CACUU...AUG-5'@LB@ \| \|@LB@ 59 48@LB@@LB@@LB@Energy -8.25 kcal/mol@LB@Hybridization Energy -10.8722 kcal/mol@LB@Unfolding Energy - mRNA 2.31397 kcal/mol@LB@Unfolding Energy - sRNA 0.318367 kcal/mol@LB@@LB@Position - mRNA 172 -- 181@LB@Position - sRNA 49 -- 58@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 49 -- 55@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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