Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
13	4.485e-05	0.01183	b0511	ybbW	-13.60	0.002717	173 -- 210	18 -- 58	putative allantoin transporter		_____________172________________________________________211\n_____________\|__________________________________________\|\n5'-UGG...ACGUU_______________AU____________UUU__________AGAGA...UUA-3'\n______________AUUCGA_UACGUGAA__UAAGAGG___GA___AUGGAACAUC\n______________\|\|:\|\|\|_\|\|\|\|\|\|\|\|__\|\|\|:\|\|\|___\|\|___\|\|:\|\|\|\|\|\|\|\n______________UAGGCU_AUGCACUU__AUUUUCC___CU___UAUCUUGUAG\n3'-AUU...CGGUU______A________C________AGA__UUC__________AAUGG...AUG-5'\n_____________\|__________________________________________\|\n_____________59_________________________________________17\n	204 -- 210	18 -- 24	-34.0515 kcal/mol	8.25655 kcal/mol	12.1809 kcal/mol	172 211@LB@ \| \|@LB@5'-UGG...ACGUU AU UUU AGAGA...UUA-3'@LB@ AUUCGA UACGUGAA UAAGAGG GA AUGGAACAUC@LB@ \|\|:\|\|\| \|\|\|\|\|\|\|\| \|\|\|:\|\|\| \|\| \|\|:\|\|\|\|\|\|\|@LB@ UAGGCU AUGCACUU AUUUUCC CU UAUCUUGUAG@LB@3'-AUU...CGGUU A C AGA UUC AAUGG...AUG-5'@LB@ \| \|@LB@ 59 17@LB@@LB@@LB@Energy -13.60 kcal/mol@LB@Hybridization Energy -34.0515 kcal/mol@LB@Unfolding Energy - mRNA 8.25655 kcal/mol@LB@Unfolding Energy - sRNA 12.1809 kcal/mol@LB@@LB@Position - mRNA 173 -- 210@LB@Position - sRNA 18 -- 58@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 18 -- 24@LB@
25	0.0002052	0.02687	b0885	aat	-14.70	0.000956	173 -- 191	42 -- 59	leucyl/phenylalanyl-tRNA-protein transferase		_____________172_________________192\n_____________\|___________________\|\n5'-CGU...UGAAC_______C___________UUUGC...UUA-3'\n______________GAUCCGA_UUGCGUGGAGU\n______________:\|\|\|\|\|\|_\|\|:\|\|\|\|:\|\|\|\n______________UUAGGCU_AAUGCACUUCA\n3'-AUU...UCGGU___________________UUUUC...AUG-5'\n_____________\|___________________\|\n_____________60__________________41\n	185 -- 191	42 -- 48	-23.9715 kcal/mol	6.00825 kcal/mol	3.27976 kcal/mol	172 192@LB@ \| \|@LB@5'-CGU...UGAAC C UUUGC...UUA-3'@LB@ GAUCCGA UUGCGUGGAGU@LB@ :\|\|\|\|\|\| \|\|:\|\|\|\|:\|\|\|@LB@ UUAGGCU AAUGCACUUCA@LB@3'-AUU...UCGGU UUUUC...AUG-5'@LB@ \| \|@LB@ 60 41@LB@@LB@@LB@Energy -14.70 kcal/mol@LB@Hybridization Energy -23.9715 kcal/mol@LB@Unfolding Energy - mRNA 6.00825 kcal/mol@LB@Unfolding Energy - sRNA 3.27976 kcal/mol@LB@@LB@Position - mRNA 173 -- 191@LB@Position - sRNA 42 -- 59@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 42 -- 48@LB@
49	0.0005477	0.03933	b2715	ascF	-9.59	0.059292	173 -- 180	1 -- 8	cellobiose/arbutin/salicin-specific PTS enzymes IIB and IC components		_____________172______181\n_____________\|________\|\n5'-UGC...ACAUC________UGAUA...GCU-3'\n______________UACCCUAC\n______________\|\|\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	174 -- 180	1 -- 7	-12.1729 kcal/mol	0.486168 kcal/mol	2.11603 kcal/mol	172 181@LB@ \| \|@LB@5'-UGC...ACAUC UGAUA...GCU-3'@LB@ UACCCUAC@LB@ \|\|\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -12.1729 kcal/mol@LB@Unfolding Energy - mRNA 0.486168 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 173 -- 180@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 1 -- 7@LB@
105	0.003348	0.1101	b2218	rcsC	-11.03	0.021992	174 -- 214	1 -- 37	hybrid sensory kinase in two-component regulatory system with RcsB and YojN		_____________173_______________________________________215\n_____________\|_________________________________________\|\n5'-AGC...CCAUC____ACCU____C_____U__GCC______AAA________UUCUU...CCG-3'\n______________GGUC____GAGG_GGAGC_UC___CCUUUG___UACCUUGC\n______________\|\|\|\|____:\|\|:_:\|\|:\|_\|\|___\|\|\|:\|\|___\|\|\|\|:\|:\|\n______________CCAG____UUCU_UCUUG_AG___GGAGAC___AUGGGAUG\n3'-AUU...AUUUU____ACU_____A_____U__AAU_________________-5'\n_____________\|________________________________________\|\n_____________38_______________________________________1\n	208 -- 214	1 -- 7	-33.3544 kcal/mol	10.9963 kcal/mol	11.3499 kcal/mol	173 215@LB@ \| \|@LB@5'-AGC...CCAUC ACCU C U GCC AAA UUCUU...CCG-3'@LB@ GGUC GAGG GGAGC UC CCUUUG UACCUUGC@LB@ \|\|\|\| :\|\|: :\|\|:\| \|\| \|\|\|:\|\| \|\|\|\|:\|:\|@LB@ CCAG UUCU UCUUG AG GGAGAC AUGGGAUG@LB@3'-AUU...AUUUU ACU A U AAU -5'@LB@ \| \|@LB@ 38 1@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -33.3544 kcal/mol@LB@Unfolding Energy - mRNA 10.9963 kcal/mol@LB@Unfolding Energy - sRNA 11.3499 kcal/mol@LB@@LB@Position - mRNA 174 -- 214@LB@Position - sRNA 1 -- 37@LB@Position Seed - mRNA 208 -- 214@LB@Position Seed - sRNA 1 -- 7@LB@
117	0.003878	0.1165	b3078	ygjI	-10.25	0.038289	174 -- 181	1 -- 8	putative transporter	b0899	_____________173______182\n_____________\|________\|\n5'-GGA...ACCGC________UCAUU...UUG-3'\n______________UACCCUAC\n______________\|\|\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	175 -- 181	1 -- 7	-12.7576 kcal/mol	0.416118 kcal/mol	2.11603 kcal/mol	173 182@LB@ \| \|@LB@5'-GGA...ACCGC UCAUU...UUG-3'@LB@ UACCCUAC@LB@ \|\|\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -10.25 kcal/mol@LB@Hybridization Energy -12.7576 kcal/mol@LB@Unfolding Energy - mRNA 0.416118 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 174 -- 181@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 1 -- 7@LB@
170	0.007042	0.1457	b3110	yhaO	-10.02	0.044877	174 -- 180	1 -- 7	putative transporter		_____________173_____181\n_____________\|_______\|\n5'-AAU...AAUAA_______AAAAU...UCG-3'\n______________GCCCUAC\n______________:\|\|\|\|\|\|\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	174 -- 180	1 -- 7	-12.6531 kcal/mol	0.621925 kcal/mol	2.03949 kcal/mol	173 181@LB@ \| \|@LB@5'-AAU...AAUAA AAAAU...UCG-3'@LB@ GCCCUAC@LB@ :\|\|\|\|\|\|@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -10.02 kcal/mol@LB@Hybridization Energy -12.6531 kcal/mol@LB@Unfolding Energy - mRNA 0.621925 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 174 -- 180@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 1 -- 7@LB@
177	0.007672	0.1511	b2307	hisM	-10.48	0.032509	175 -- 237	1 -- 57	histidine ABC transporter permease		_____________174__________________________________________________________________238\n_____________\|____________________________________________________________________\|\n5'-CUC...GCUAC____UGGG___________________CCUGU___C___________AUGAAUA___GAA___G____UGUGG...CGG-3'\n______________UCCG____UGUGAAG___AGGG_CUGA_____GAU_GAA_AUCUUAC_______CUG___ACC_CUGC\n______________\|\|\|\|____:\|\|\|\|\|\|___\|:\|\|_\|\|\|\|_____\|\|\|_\|\|\|_\|\|\|\|\|\|\|_______\|\|\|___\|\|\|_\|\|:\|\n______________AGGC____GCACUUC___UUCC_GACU_____CUA_CUU_UAGAAUG_______GAC___UGG_GAUG\n3'-AUU...GGUUU____UAAU_______AUU____A____UU______U___G_______GA________A__________-5'\n_____________\|___________________________________________________________________\|\n_____________58__________________________________________________________________1\n	210 -- 216	14 -- 20	-38.5224 kcal/mol	15.358 kcal/mol	12.7026 kcal/mol	174 238@LB@ \| \|@LB@5'-CUC...GCUAC UGGG CCUGU C AUGAAUA GAA G UGUGG...CGG-3'@LB@ UCCG UGUGAAG AGGG CUGA GAU GAA AUCUUAC CUG ACC CUGC@LB@ \|\|\|\| :\|\|\|\|\|\| \|:\|\| \|\|\|\| \|\|\| \|\|\| \|\|\|\|\|\|\| \|\|\| \|\|\| \|\|:\|@LB@ AGGC GCACUUC UUCC GACU CUA CUU UAGAAUG GAC UGG GAUG@LB@3'-AUU...GGUUU UAAU AUU A UU U G GA A -5'@LB@ \| \|@LB@ 58 1@LB@@LB@@LB@Energy -10.48 kcal/mol@LB@Hybridization Energy -38.5224 kcal/mol@LB@Unfolding Energy - mRNA 15.358 kcal/mol@LB@Unfolding Energy - sRNA 12.7026 kcal/mol@LB@@LB@Position - mRNA 175 -- 237@LB@Position - sRNA 1 -- 57@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 14 -- 20@LB@
52	0.0006736	0.04557	b3964	yijD	-9.23	0.074829	177 -- 192	43 -- 57	DUF1422 family inner membrane protein		_____________176______________193\n_____________\|________________\|\n5'-ACU...AUUAU_____GAA________GACGA...UGC-3'\n______________UCCGG___AUGUGAAG\n______________\|\|\|\|:___\|:\|\|\|\|\|\|\n______________AGGCU___UGCACUUC\n3'-AUU...GGUUU_____AA_________AUUUU...AUG-5'\n_____________\|________________\|\n_____________58_______________42\n	186 -- 192	43 -- 49	-16.7307 kcal/mol	4.24116 kcal/mol	3.27382 kcal/mol	176 193@LB@ \| \|@LB@5'-ACU...AUUAU GAA GACGA...UGC-3'@LB@ UCCGG AUGUGAAG@LB@ \|\|\|\|: \|:\|\|\|\|\|\|@LB@ AGGCU UGCACUUC@LB@3'-AUU...GGUUU AA AUUUU...AUG-5'@LB@ \| \|@LB@ 58 42@LB@@LB@@LB@Energy -9.23 kcal/mol@LB@Hybridization Energy -16.7307 kcal/mol@LB@Unfolding Energy - mRNA 4.24116 kcal/mol@LB@Unfolding Energy - sRNA 3.27382 kcal/mol@LB@@LB@Position - mRNA 177 -- 192@LB@Position - sRNA 43 -- 57@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 43 -- 49@LB@
90	0.002406	0.09404	b0551	quuD	-13.47	0.003063	177 -- 196	40 -- 58	DLP12 prophage; putative antitermination protein		_____________176__________________197\n_____________\|____________________\|\n5'-ACU...UUCUG________UU__________GUUGA...GAU-3'\n______________GUCUGAUU__UGUGGAGUAA\n______________:\|\|:\|\|\|\|__:\|\|\|:\|\|\|\|\|\n______________UAGGCUAA__GCACUUCAUU\n3'-AUU...CGGUU________U___________UUCCA...AUG-5'\n_____________\|____________________\|\n_____________59___________________39\n	190 -- 196	40 -- 46	-19.625 kcal/mol	2.82746 kcal/mol	3.34877 kcal/mol	176 197@LB@ \| \|@LB@5'-ACU...UUCUG UU GUUGA...GAU-3'@LB@ GUCUGAUU UGUGGAGUAA@LB@ :\|\|:\|\|\|\| :\|\|\|:\|\|\|\|\|@LB@ UAGGCUAA GCACUUCAUU@LB@3'-AUU...CGGUU U UUCCA...AUG-5'@LB@ \| \|@LB@ 59 39@LB@@LB@@LB@Energy -13.47 kcal/mol@LB@Hybridization Energy -19.625 kcal/mol@LB@Unfolding Energy - mRNA 2.82746 kcal/mol@LB@Unfolding Energy - sRNA 3.34877 kcal/mol@LB@@LB@Position - mRNA 177 -- 196@LB@Position - sRNA 40 -- 58@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 40 -- 46@LB@
93	0.002634	0.09947	b2202	napC	-9.04	0.084220	177 -- 197	34 -- 54	quinol dehydrogenase electron source for NapAB		_____________176___________________198\n_____________\|_____________________\|\n5'-ACG...CAAAA_______G___A_________AUUAU...GUU-3'\n______________GGUUACG_GAA_UAAGAGGUU\n______________\|:\|\|\|\|\|_\|\|\|_\|\|\|:\|\|\|\|:\n______________CUAAUGC_CUU_AUUUUCCAG\n3'-AUU...UUAGG_______A___C_________ACUUU...AUG-5'\n_____________\|_____________________\|\n_____________55____________________33\n	177 -- 183	48 -- 54	-21.2793 kcal/mol	4.43682 kcal/mol	7.71857 kcal/mol	176 198@LB@ \| \|@LB@5'-ACG...CAAAA G A AUUAU...GUU-3'@LB@ GGUUACG GAA UAAGAGGUU@LB@ \|:\|\|\|\|\| \|\|\| \|\|\|:\|\|\|\|:@LB@ CUAAUGC CUU AUUUUCCAG@LB@3'-AUU...UUAGG A C ACUUU...AUG-5'@LB@ \| \|@LB@ 55 33@LB@@LB@@LB@Energy -9.04 kcal/mol@LB@Hybridization Energy -21.2793 kcal/mol@LB@Unfolding Energy - mRNA 4.43682 kcal/mol@LB@Unfolding Energy - sRNA 7.71857 kcal/mol@LB@@LB@Position - mRNA 177 -- 197@LB@Position - sRNA 34 -- 54@LB@Position Seed - mRNA 177 -- 183@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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