Input and runtime details for job 7819033 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 7819033 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:17 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:46 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:05:24 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:05:32 CET 2018
	Job Completed		@ Fri Feb 16 21:05:57 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=7819033&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicC

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
2	3.183e-05	0.03921	b0889	lrp	-15.78	0.004789	150 -- 212	1 -- 70	leucine-responsive global transcriptional regulator	_____________149__________________________________________________________________________213\n_____________\|____________________________________________________________________________\|\n5'-GUG...CAGUG_____UC________ACA______A_G__A__AAU___________________________UAAU____G_____AAGAA...AGC-3'\n______________AUGUU__AGGGUCAG___GG_AGU_G_GA_GG____ACAGAGA___________GACAAUAA____GGUA_AUAGC\n______________\|\|\|\|\|__\|:::\|\|\|\|___\|\|_\|:\|_\|_:\|_\|\|____\|\|\|\|\|:\|___________\|\|\|\|\|\|\|\|____\|\|:\|_\|\|\|:\|\n______________UACAA__UUUUAGUC___CC_UUA_C_UU_CC____UGUCUUU___________CUGUUAUU____CCGU_UAUUG\n3'-UUU...AAAUA_____CC________A____G_____G__A__GGGU_______UAUUUUAGACA________U_______A_____-5'\n_____________\|___________________________________________________________________________\|\n_____________71__________________________________________________________________________1\n	192 -- 198	12 -- 18	-35.9997 kcal/mol	8.06188 kcal/mol	12.1618 kcal/mol	149 213@LB@ \| \|@LB@5'-GUG...CAGUG UC ACA A G A AAU UAAU G AAGAA...AGC-3'@LB@ AUGUU AGGGUCAG GG AGU G GA GG ACAGAGA GACAAUAA GGUA AUAGC@LB@ \|\|\|\|\| \|:::\|\|\|\| \|\| \|:\| \| :\| \|\| \|\|\|\|\|:\| \|\|\|\|\|\|\|\| \|\|:\| \|\|\|:\|@LB@ UACAA UUUUAGUC CC UUA C UU CC UGUCUUU CUGUUAUU CCGU UAUUG@LB@3'-UUU...AAAUA CC A G G A GGGU UAUUUUAGACA U A -5'@LB@ \| \|@LB@ 71 1@LB@@LB@@LB@Energy -15.78 kcal/mol@LB@Hybridization Energy -35.9997 kcal/mol@LB@Unfolding Energy - mRNA 8.06188 kcal/mol@LB@Unfolding Energy - sRNA 12.1618 kcal/mol@LB@@LB@Position - mRNA 150 -- 212@LB@Position - sRNA 1 -- 70@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 12 -- 18@LB@
4	6.145e-05	0.05167	b4217	ytfK	-14.33	0.011783	150 -- 205	11 -- 68	DUF1107 family protein	_____________149________________________________________________________________206\n_____________\|__________________________________________________________________\|\n5'-GAA...UAAUA______CC___________CC___CA___GUAAUAC______________________________AAUUU...AAU-3'\n______________GUUGGG__GAUU_GUGGCA__GCA__GGC_______UCAGCAGGAGAUA_________ACAAUGAA\n______________\|\|\|\|\|:__:\|\|:_\|\|\|\|\|\|__\|\|\|__\|\|\|_______:\|\|:\|\|\|:\|:\|\|\|_________\|\|\|\|\|:\|\|\n______________CAACCU__UUAG_CACCGU__CGU__CCG_______GGUUGUCUUUUAU_________UGUUAUUU\n3'-UUU...AUAUA______U_____U______UA___UA_______________________UUUAGACAC________CCGUA...UUG-5'\n_____________\|__________________________________________________________________\|\n_____________69_________________________________________________________________10\n	199 -- 205	11 -- 17	-43.2853 kcal/mol	16.8726 kcal/mol	12.0809 kcal/mol	149 206@LB@ \| \|@LB@5'-GAA...UAAUA CC CC CA GUAAUAC AAUUU...AAU-3'@LB@ GUUGGG GAUU GUGGCA GCA GGC UCAGCAGGAGAUA ACAAUGAA@LB@ \|\|\|\|\|: :\|\|: \|\|\|\|\|\| \|\|\| \|\|\| :\|\|:\|\|\|:\|:\|\|\| \|\|\|\|\|:\|\|@LB@ CAACCU UUAG CACCGU CGU CCG GGUUGUCUUUUAU UGUUAUUU@LB@3'-UUU...AUAUA U U UA UA UUUAGACAC CCGUA...UUG-5'@LB@ \| \|@LB@ 69 10@LB@@LB@@LB@Energy -14.33 kcal/mol@LB@Hybridization Energy -43.2853 kcal/mol@LB@Unfolding Energy - mRNA 16.8726 kcal/mol@LB@Unfolding Energy - sRNA 12.0809 kcal/mol@LB@@LB@Position - mRNA 150 -- 205@LB@Position - sRNA 11 -- 68@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 11 -- 17@LB@
5	0.0001064	0.07159	b1784	yeaH	-10.63	0.089613	150 -- 205	1 -- 57	UPF0229 family protein	_____________149________________________________________________________206\n_____________\|__________________________________________________________\|\n5'-UGU...UGAGA__C_____CA_______G_____UACAGUUGGCAAA___AGUACG_____________CUGGU...CGA-3'\n______________AG_GGCAA__CA__GGC_UAGCA_____________UGU______GGGGGCAUAUGAC\n______________\|\|_\|\|\|\|\|__\|\|__\|\|\|_:\|:\|\|_____________\|\|\|______:::\|\|\|\|\|\|\|:\|\|\n______________UC_CCGUU__GU__CCG_GUUGU_____________ACA______UUUCCGUAUAUUG\n3'-UUU...UUUAG__A_____AC__UA___G_____CUUUUAUUUUAG____CUGUUA_____________-5'\n_____________\|_________________________________________________________\|\n_____________58________________________________________________________1\n	198 -- 204	2 -- 8	-36.8439 kcal/mol	14.0882 kcal/mol	12.1233 kcal/mol	149 206@LB@ \| \|@LB@5'-UGU...UGAGA C CA G UACAGUUGGCAAA AGUACG CUGGU...CGA-3'@LB@ AG GGCAA CA GGC UAGCA UGU GGGGGCAUAUGAC@LB@ \|\| \|\|\|\|\| \|\| \|\|\| :\|:\|\| \|\|\| :::\|\|\|\|\|\|\|:\|\|@LB@ UC CCGUU GU CCG GUUGU ACA UUUCCGUAUAUUG@LB@3'-UUU...UUUAG A AC UA G CUUUUAUUUUAG CUGUUA -5'@LB@ \| \|@LB@ 58 1@LB@@LB@@LB@Energy -10.63 kcal/mol@LB@Hybridization Energy -36.8439 kcal/mol@LB@Unfolding Energy - mRNA 14.0882 kcal/mol@LB@Unfolding Energy - sRNA 12.1233 kcal/mol@LB@@LB@Position - mRNA 150 -- 205@LB@Position - sRNA 1 -- 57@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 2 -- 8@LB@
67	0.005347	0.2645	b4278	insG	-9.22	0.173962	151 -- 164	45 -- 58	IS4 transposase	_____________150____________165\n_____________\|______________\|\n5'-GUU...CGAUC_______U______GAAUC...CUC-3'\n______________CAGUGGC_GUGUAA\n______________\|\|\|\|\|\|\|_:\|\|:\|\|\n______________GUCACCG_UACGUU\n3'-UUU...UUUUA_______U______ACCGG...UUG-5'\n_____________\|______________\|\n_____________59_____________44\n	151 -- 157	52 -- 58	-18.8848 kcal/mol	6.17717 kcal/mol	3.48403 kcal/mol	150 165@LB@ \| \|@LB@5'-GUU...CGAUC U GAAUC...CUC-3'@LB@ CAGUGGC GUGUAA@LB@ \|\|\|\|\|\|\| :\|\|:\|\|@LB@ GUCACCG UACGUU@LB@3'-UUU...UUUUA U ACCGG...UUG-5'@LB@ \| \|@LB@ 59 44@LB@@LB@@LB@Energy -9.22 kcal/mol@LB@Hybridization Energy -18.8848 kcal/mol@LB@Unfolding Energy - mRNA 6.17717 kcal/mol@LB@Unfolding Energy - sRNA 3.48403 kcal/mol@LB@@LB@Position - mRNA 151 -- 164@LB@Position - sRNA 45 -- 58@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 52 -- 58@LB@
34	0.002116	0.2088	b0081	mraZ	-11.86	0.047767	154 -- 189	9 -- 59	RsmH methytransferase inhibitor	_____________153___________________________________________________190\n_____________\|_____________________________________________________\|\n5'-GAU...UCCUU_______G__AU_________AA_____________________G________GUGAG...AAA-3'\n______________UCAGUGG_AA__UGUGG_GGC__A________________GUGG_AAUAAGGG\n______________\|\|\|\|\|\|\|_\|\|__\|\|:::_\|\|\|__\|________________\|\|\|:_\|\|\|\|\|:\|\|\n______________AGUCACC_UU__ACGUU_CCG__U________________CACU_UUAUUUCC\n3'-UUU...CUUUU_______G_________A___GG_UGUCUUUUAUUUUAGA____G________GUAUAUUG-5'\n_____________\|_____________________________________________________\|\n_____________60____________________________________________________8\n	183 -- 189	9 -- 15	-29.7093 kcal/mol	6.7157 kcal/mol	11.1344 kcal/mol	153 190@LB@ \| \|@LB@5'-GAU...UCCUU G AU AA G GUGAG...AAA-3'@LB@ UCAGUGG AA UGUGG GGC A GUGG AAUAAGGG@LB@ \|\|\|\|\|\|\| \|\| \|\|::: \|\|\| \| \|\|\|: \|\|\|\|\|:\|\|@LB@ AGUCACC UU ACGUU CCG U CACU UUAUUUCC@LB@3'-UUU...CUUUU G A GG UGUCUUUUAUUUUAGA G GUAUAUUG-5'@LB@ \| \|@LB@ 60 8@LB@@LB@@LB@Energy -11.86 kcal/mol@LB@Hybridization Energy -29.7093 kcal/mol@LB@Unfolding Energy - mRNA 6.7157 kcal/mol@LB@Unfolding Energy - sRNA 11.1344 kcal/mol@LB@@LB@Position - mRNA 154 -- 189@LB@Position - sRNA 9 -- 59@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 9 -- 15@LB@
66	0.005339	0.2645	b1476	fdnI	-10.79	0.082799	156 -- 194	9 -- 48	formate dehydrogenase-N cytochrome B556 (gamma) subunit nitrate-inducible	_____________155________________________________________195\n_____________\|__________________________________________\|\n5'-CGU...CAUCG___U________AUA_____________GA______G_____AUCAU...UUU-3'\n______________GUA_UGGCCCGA___AGGAAGUGGA__C__UGACGA__GAGG\n______________\|:\|_\|\|\|\|\|\|:\|___\|\|:\|\|:\|::\|__\|__\|\|\|\|:\|__:\|\|\|\n______________CGU_ACCGGGUU___UCUUUUAUUU__G__ACUGUU__UUCC\n3'-UUU...CGUUA___U________G____________UA_AC______AU____GUAUAUUG-5'\n_____________\|__________________________________________\|\n_____________49_________________________________________8\n	161 -- 167	37 -- 43	-34.1322 kcal/mol	12.2398 kcal/mol	11.1008 kcal/mol	155 195@LB@ \| \|@LB@5'-CGU...CAUCG U AUA GA G AUCAU...UUU-3'@LB@ GUA UGGCCCGA AGGAAGUGGA C UGACGA GAGG@LB@ \|:\| \|\|\|\|\|\|:\| \|\|:\|\|:\|::\| \| \|\|\|\|:\| :\|\|\|@LB@ CGU ACCGGGUU UCUUUUAUUU G ACUGUU UUCC@LB@3'-UUU...CGUUA U G UA AC AU GUAUAUUG-5'@LB@ \| \|@LB@ 49 8@LB@@LB@@LB@Energy -10.79 kcal/mol@LB@Hybridization Energy -34.1322 kcal/mol@LB@Unfolding Energy - mRNA 12.2398 kcal/mol@LB@Unfolding Energy - sRNA 11.1008 kcal/mol@LB@@LB@Position - mRNA 156 -- 194@LB@Position - sRNA 9 -- 48@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 37 -- 43@LB@
83	0.007014	0.2822	b0815	opgE	-11.11	0.070482	164 -- 204	2 -- 58	OPG biosynthetic transmembrane phosphoethanolamine transferase	_____________163________________________________________________________205\n_____________\|__________________________________________________________\|\n5'-UUU...GACGC_____________C__________________________U_______U_________AUUUA...GCU-3'\n______________CAG_GGUAA_GCA_AUGGC_______________GUUUGU_ACGAUAG_GGCAUAUGA\n______________\|\|\|_\|\|:\|\|_\|\|\|_\|\|\|\|\|_______________:\|:\|\|\|_\|\|:\|\|\|:_\|\|\|\|\|\|\|:\|\n______________GUC_CCGUU_CGU_UACCG_______________UAGACA_UGUUAUU_CCGUAUAUU\n3'-UUU...UUUUA___A_____A_________GGUUGUCUUUUAUUU______C_______U_________G-5'\n_____________\|__________________________________________________________\|\n_____________59_________________________________________________________1\n	197 -- 203	3 -- 9	-40.5172 kcal/mol	17.2586 kcal/mol	12.1475 kcal/mol	163 205@LB@ \| \|@LB@5'-UUU...GACGC C U U AUUUA...GCU-3'@LB@ CAG GGUAA GCA AUGGC GUUUGU ACGAUAG GGCAUAUGA@LB@ \|\|\| \|\|:\|\| \|\|\| \|\|\|\|\| :\|:\|\|\| \|\|:\|\|\|: \|\|\|\|\|\|\|:\|@LB@ GUC CCGUU CGU UACCG UAGACA UGUUAUU CCGUAUAUU@LB@3'-UUU...UUUUA A A GGUUGUCUUUUAUUU C U G-5'@LB@ \| \|@LB@ 59 1@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -40.5172 kcal/mol@LB@Unfolding Energy - mRNA 17.2586 kcal/mol@LB@Unfolding Energy - sRNA 12.1475 kcal/mol@LB@@LB@Position - mRNA 164 -- 204@LB@Position - sRNA 2 -- 58@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 3 -- 9@LB@
87	0.007653	0.2889	b1881	cheZ	-13.56	0.018568	164 -- 190	2 -- 28	chemotaxis regulator protein phosphatase for CheY	_____________163_________________________191\n_____________\|___________________________\|\n5'-AUU...UCAAC_______U___G__ACU__________GGAUG...GGG-3'\n______________AAAAUCU_UGA_AA___GGGCAUGUGA\n______________\|\|\|\|\|\|\|_\|\|\|_\|\|___:\|\|\|\|\|:\|:\|\n______________UUUUAGA_ACU_UU___UCCGUAUAUU\n3'-UUU...UUUUA_______C___G__AUU__________G-5'\n_____________\|___________________________\|\n_____________29__________________________1\n	183 -- 189	3 -- 9	-22.696 kcal/mol	4.24203 kcal/mol	4.89261 kcal/mol	163 191@LB@ \| \|@LB@5'-AUU...UCAAC U G ACU GGAUG...GGG-3'@LB@ AAAAUCU UGA AA GGGCAUGUGA@LB@ \|\|\|\|\|\|\| \|\|\| \|\| :\|\|\|\|\|:\|:\|@LB@ UUUUAGA ACU UU UCCGUAUAUU@LB@3'-UUU...UUUUA C G AUU G-5'@LB@ \| \|@LB@ 29 1@LB@@LB@@LB@Energy -13.56 kcal/mol@LB@Hybridization Energy -22.696 kcal/mol@LB@Unfolding Energy - mRNA 4.24203 kcal/mol@LB@Unfolding Energy - sRNA 4.89261 kcal/mol@LB@@LB@Position - mRNA 164 -- 190@LB@Position - sRNA 2 -- 28@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 3 -- 9@LB@
27	0.001616	0.2012	b0085	murE	-13.53	0.018874	165 -- 198	1 -- 33	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso- diaminopimelate ligase	_____________164_________________________________199\n_____________\|___________________________________\|\n5'-UGC...CGAUA_________________U___C______AGGG___AGGUG...CGG-3'\n______________AAAAU_GAAUUUGUGAU_AAU_AAGGCG____GAC\n______________\|\|\|\|\|_:\|\|\|:\|\|\|\|\|:_\|\|\|_\|\|\|\|\|:____:\|\|\n______________UUUUA_UUUAGACACUG_UUA_UUCCGU____UUG\n3'-UUU...UUGUC_____U_______________U______AUA____-5'\n_____________\|__________________________________\|\n_____________34_________________________________1\n	174 -- 180	17 -- 23	-22.2929 kcal/mol	3.71145 kcal/mol	5.04823 kcal/mol	164 199@LB@ \| \|@LB@5'-UGC...CGAUA U C AGGG AGGUG...CGG-3'@LB@ AAAAU GAAUUUGUGAU AAU AAGGCG GAC@LB@ \|\|\|\|\| :\|\|\|:\|\|\|\|\|: \|\|\| \|\|\|\|\|: :\|\|@LB@ UUUUA UUUAGACACUG UUA UUCCGU UUG@LB@3'-UUU...UUGUC U U AUA -5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -13.53 kcal/mol@LB@Hybridization Energy -22.2929 kcal/mol@LB@Unfolding Energy - mRNA 3.71145 kcal/mol@LB@Unfolding Energy - sRNA 5.04823 kcal/mol@LB@@LB@Position - mRNA 165 -- 198@LB@Position - sRNA 1 -- 33@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 17 -- 23@LB@
165	0.01953	0.3981	b0603	ybdO	-12.89	0.027143	166 -- 206	40 -- 73	putative DNA-binding transcriptional regulator	_____________165_________________________________________207\n_____________\|___________________________________________\|\n5'-UCA...GUGAA_____CA_______AAAAUAUAAC___A_______________AAUCU...CAC-3'\n______________UAUAU__UGGAAGA__________CGG__GUAGUGUA_UGGCC\n______________\|\|\|\|\|__\|\|\|\|\|:\|__________\|:\|__\|:\|:\|\|:\|_\|\|\|\|\|\n______________AUAUA__ACCUUUU__________GUC__CGUUACGU_ACCGG\n3'-UUU...GAAAA_____CA_______A____________AC________U_____GUUGU...UUG-5'\n_____________\|___________________________________________\|\n_____________74__________________________________________39\n	195 -- 201	46 -- 52	-24.6879 kcal/mol	4.92423 kcal/mol	6.86861 kcal/mol	165 207@LB@ \| \|@LB@5'-UCA...GUGAA CA AAAAUAUAAC A AAUCU...CAC-3'@LB@ UAUAU UGGAAGA CGG GUAGUGUA UGGCC@LB@ \|\|\|\|\| \|\|\|\|\|:\| \|:\| \|:\|:\|\|:\| \|\|\|\|\|@LB@ AUAUA ACCUUUU GUC CGUUACGU ACCGG@LB@3'-UUU...GAAAA CA A AC U GUUGU...UUG-5'@LB@ \| \|@LB@ 74 39@LB@@LB@@LB@Energy -12.89 kcal/mol@LB@Hybridization Energy -24.6879 kcal/mol@LB@Unfolding Energy - mRNA 4.92423 kcal/mol@LB@Unfolding Energy - sRNA 6.86861 kcal/mol@LB@@LB@Position - mRNA 166 -- 206@LB@Position - sRNA 40 -- 73@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 46 -- 52@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching