Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
107	0.02168	0.6177	b1738	chbB	-9.32	0.056668	26 -- 34	47 -- 55	N N'-diacetylchitobiose-specific enzyme IIB component of PTS	_____________25________35\n_____________\|_________\|\n5'-AAU...CACAG_________AAACA...CGG-3'\n______________GUCAAGGAG\n______________\|\|\|\|\|:\|\|\|\n______________CAGUUUCUC\n3'-UUU...CCGGG_________CUUAA...ACA-5'\n_____________\|_________\|\n_____________56________46\n	28 -- 34	47 -- 53	-13.6925 kcal/mol	3.47679 kcal/mol	0.899516 kcal/mol	25 35@LB@ \| \|@LB@5'-AAU...CACAG AAACA...CGG-3'@LB@ GUCAAGGAG@LB@ \|\|\|\|\|:\|\|\|@LB@ CAGUUUCUC@LB@3'-UUU...CCGGG CUUAA...ACA-5'@LB@ \| \|@LB@ 56 46@LB@@LB@@LB@Energy -9.32 kcal/mol@LB@Hybridization Energy -13.6925 kcal/mol@LB@Unfolding Energy - mRNA 3.47679 kcal/mol@LB@Unfolding Energy - sRNA 0.899516 kcal/mol@LB@@LB@Position - mRNA 26 -- 34@LB@Position - sRNA 47 -- 55@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 47 -- 53@LB@
41	0.008018	0.6129	b1740	nadE	-11.27	0.022577	258 -- 272	38 -- 52	NAD synthetase NH3/glutamine-dependent	_____________257_____________273\n_____________\|_______________\|\n5'-UUC...AUGCU_________A_____CGUAG...ACC-3'\n______________GAAGAGGAA_UUCGU\n______________:\|\|\|\|\|\|\|\|_\|\|\|:\|\n______________UUUCUCCUU_AAGUA\n3'-UUU...GGCAG_________A_____AAAAA...ACA-5'\n_____________\|_______________\|\n_____________53______________37\n	260 -- 266	44 -- 50	-15.8385 kcal/mol	3.49717 kcal/mol	1.0693 kcal/mol	257 273@LB@ \| \|@LB@5'-UUC...AUGCU A CGUAG...ACC-3'@LB@ GAAGAGGAA UUCGU@LB@ :\|\|\|\|\|\|\|\| \|\|\|:\|@LB@ UUUCUCCUU AAGUA@LB@3'-UUU...GGCAG A AAAAA...ACA-5'@LB@ \| \|@LB@ 53 37@LB@@LB@@LB@Energy -11.27 kcal/mol@LB@Hybridization Energy -15.8385 kcal/mol@LB@Unfolding Energy - mRNA 3.49717 kcal/mol@LB@Unfolding Energy - sRNA 1.0693 kcal/mol@LB@@LB@Position - mRNA 258 -- 272@LB@Position - sRNA 38 -- 52@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 44 -- 50@LB@
176	0.03596	0.6177	b1747	astA	-4.82	0.399998	129 -- 139	46 -- 56	arginine succinyltransferase	_____________128_________140\n_____________\|___________\|\n5'-GGC...GUCAG__AA_______UGACG...AAG-3'\n______________CG__GAAGAGG\n______________\|\|__:\|\|\|\|\|\|\n______________GC__UUUCUCC\n3'-UUU...ACCGG__AG_______UUAAA...ACA-5'\n_____________\|___________\|\n_____________57__________45\n	133 -- 139	46 -- 52	-9.86744 kcal/mol	4.11365 kcal/mol	0.93204 kcal/mol	128 140@LB@ \| \|@LB@5'-GGC...GUCAG AA UGACG...AAG-3'@LB@ CG GAAGAGG@LB@ \|\| :\|\|\|\|\|\|@LB@ GC UUUCUCC@LB@3'-UUU...ACCGG AG UUAAA...ACA-5'@LB@ \| \|@LB@ 57 45@LB@@LB@@LB@Energy -4.82 kcal/mol@LB@Hybridization Energy -9.86744 kcal/mol@LB@Unfolding Energy - mRNA 4.11365 kcal/mol@LB@Unfolding Energy - sRNA 0.93204 kcal/mol@LB@@LB@Position - mRNA 129 -- 139@LB@Position - sRNA 46 -- 56@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 46 -- 52@LB@
26	0.004859	0.5992	b1753	ynjA	-8.90	0.068656	186 -- 195	43 -- 52	carboxymuconolactone decarboxylase family protein	_____________185________196\n_____________\|__________\|\n5'-ACU...CGCCA__________GCCUG...GGU-3'\n______________GAAGAGGAAU\n______________:\|\|\|\|\|\|\|\|\|\n______________UUUCUCCUUA\n3'-UUU...GGCAG__________AAGUA...ACA-5'\n_____________\|__________\|\n_____________53_________42\n	189 -- 195	43 -- 49	-11.6028 kcal/mol	1.83308 kcal/mol	0.865937 kcal/mol	185 196@LB@ \| \|@LB@5'-ACU...CGCCA GCCUG...GGU-3'@LB@ GAAGAGGAAU@LB@ :\|\|\|\|\|\|\|\|\|@LB@ UUUCUCCUUA@LB@3'-UUU...GGCAG AAGUA...ACA-5'@LB@ \| \|@LB@ 53 42@LB@@LB@@LB@Energy -8.90 kcal/mol@LB@Hybridization Energy -11.6028 kcal/mol@LB@Unfolding Energy - mRNA 1.83308 kcal/mol@LB@Unfolding Energy - sRNA 0.865937 kcal/mol@LB@@LB@Position - mRNA 186 -- 195@LB@Position - sRNA 43 -- 52@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 43 -- 49@LB@
106	0.02162	0.6177	b1831	proQ	-7.28	0.143813	159 -- 202	38 -- 75	RNA chaperone putative ProP translation regulator	_____________158_____________________________________________203\n_____________\|_______________________________________________\|\n5'-UUA...UUCAU___UG______U________A____CGUUGU__UC___A________GGAAA...CGC-3'\n______________GCC_____CGC_UGUUGGCU_CGUC______AA__AGG_AAUUUCAU\n______________\|\|\|_____\|\|\|_\|:\|\|\|\|\|:_\|\|\|\|______\|\|__\|\|\|_\|\|\|\|\|\|\|\|\n______________CGG_____GCG_AUAACCGG_GCAG______UU__UCC_UUAAAGUA\n3'-UUU...UUUAC___UUAUA_________________________UC____________AAAAA...ACA-5'\n_____________\|_______________________________________________\|\n_____________76______________________________________________37\n	196 -- 202	38 -- 44	-31.8607 kcal/mol	10.974 kcal/mol	13.6034 kcal/mol	158 203@LB@ \| \|@LB@5'-UUA...UUCAU UG U A CGUUGU UC A GGAAA...CGC-3'@LB@ GCC CGC UGUUGGCU CGUC AA AGG AAUUUCAU@LB@ \|\|\| \|\|\| \|:\|\|\|\|\|: \|\|\|\| \|\| \|\|\| \|\|\|\|\|\|\|\|@LB@ CGG GCG AUAACCGG GCAG UU UCC UUAAAGUA@LB@3'-UUU...UUUAC UUAUA UC AAAAA...ACA-5'@LB@ \| \|@LB@ 76 37@LB@@LB@@LB@Energy -7.28 kcal/mol@LB@Hybridization Energy -31.8607 kcal/mol@LB@Unfolding Energy - mRNA 10.974 kcal/mol@LB@Unfolding Energy - sRNA 13.6034 kcal/mol@LB@@LB@Position - mRNA 159 -- 202@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 38 -- 44@LB@
76	0.01599	0.6177	b1849	purT	-9.18	0.060248	187 -- 212	25 -- 50	phosphoribosylglycinamide formyltransferase 2	_____________186________________________213\n_____________\|__________________________\|\n5'-UUU...UAAUC______UA______AUGAC_______GGCAC...GUC-3'\n______________AGGGGA__UUCGUU_____GUUAUUA\n______________\|\|:\|\|\|__\|\|\|:\|\|_____:\|\|\|\|\|\|\n______________UCUCCU__AAGUAA_____UAAUAAU\n3'-UUU...CAGUU______UA______AAAAA_______ACGGC...ACA-5'\n_____________\|__________________________\|\n_____________51_________________________24\n	206 -- 212	25 -- 31	-15.1619 kcal/mol	4.88468 kcal/mol	1.09239 kcal/mol	186 213@LB@ \| \|@LB@5'-UUU...UAAUC UA AUGAC GGCAC...GUC-3'@LB@ AGGGGA UUCGUU GUUAUUA@LB@ \|\|:\|\|\| \|\|\|:\|\| :\|\|\|\|\|\|@LB@ UCUCCU AAGUAA UAAUAAU@LB@3'-UUU...CAGUU UA AAAAA ACGGC...ACA-5'@LB@ \| \|@LB@ 51 24@LB@@LB@@LB@Energy -9.18 kcal/mol@LB@Hybridization Energy -15.1619 kcal/mol@LB@Unfolding Energy - mRNA 4.88468 kcal/mol@LB@Unfolding Energy - sRNA 1.09239 kcal/mol@LB@@LB@Position - mRNA 187 -- 212@LB@Position - sRNA 25 -- 50@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 25 -- 31@LB@
6	0.0006545	0.351	b1857	znuA	-11.78	0.017745	58 -- 67	45 -- 54	zinc ABC transporter periplasmic binding protein	_____________57_________68\n_____________\|__________\|\n5'-GCG...ACACA__________CGCGG...AAC-3'\n______________UCAGAGAGGA\n______________\|\|\|:\|\|\|\|\|\|\n______________AGUUUCUCCU\n3'-UUU...CGGGC__________UAAAG...ACA-5'\n_____________\|__________\|\n_____________55_________44\n	61 -- 67	45 -- 51	-14.7441 kcal/mol	2.03423 kcal/mol	0.929144 kcal/mol	57 68@LB@ \| \|@LB@5'-GCG...ACACA CGCGG...AAC-3'@LB@ UCAGAGAGGA@LB@ \|\|\|:\|\|\|\|\|\|@LB@ AGUUUCUCCU@LB@3'-UUU...CGGGC UAAAG...ACA-5'@LB@ \| \|@LB@ 55 44@LB@@LB@@LB@Energy -11.78 kcal/mol@LB@Hybridization Energy -14.7441 kcal/mol@LB@Unfolding Energy - mRNA 2.03423 kcal/mol@LB@Unfolding Energy - sRNA 0.929144 kcal/mol@LB@@LB@Position - mRNA 58 -- 67@LB@Position - sRNA 45 -- 54@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 45 -- 51@LB@
171	0.0346	0.6177	b1865	nudB	-7.50	0.130652	189 -- 196	46 -- 53	dihydroneopterin triphosphate pyrophosphatase	_____________188______197\n_____________\|________\|\n5'-UGA...GUCAA________CAGCG...CCG-3'\n______________CGAAGAGG\n______________\|:\|\|\|\|\|\|\n______________GUUUCUCC\n3'-UUU...GGGCA________UUAAA...ACA-5'\n_____________\|________\|\n_____________54_______45\n	190 -- 196	46 -- 52	-10.7995 kcal/mol	2.39118 kcal/mol	0.910679 kcal/mol	188 197@LB@ \| \|@LB@5'-UGA...GUCAA CAGCG...CCG-3'@LB@ CGAAGAGG@LB@ \|:\|\|\|\|\|\|@LB@ GUUUCUCC@LB@3'-UUU...GGGCA UUAAA...ACA-5'@LB@ \| \|@LB@ 54 45@LB@@LB@@LB@Energy -7.50 kcal/mol@LB@Hybridization Energy -10.7995 kcal/mol@LB@Unfolding Energy - mRNA 2.39118 kcal/mol@LB@Unfolding Energy - sRNA 0.910679 kcal/mol@LB@@LB@Position - mRNA 189 -- 196@LB@Position - sRNA 46 -- 53@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 46 -- 52@LB@
33	0.006504	0.6129	b1937	fliE	-6.45	0.207626	119 -- 135	24 -- 40	flagellar basal-body component	_____________118_______________136\n_____________\|_________________\|\n5'-CUG...AAACC_____________C___UUAGC...GUU-3'\n______________CGUUUUUUUGUUG_UAU\n______________\|:\|\|\|\|\|\|\|:\|\|:_\|\|\|\n______________GUAAAAAAAUAAU_AUA\n3'-UUU...UUAAA_____________A___CGGCA...ACA-5'\n_____________\|_________________\|\n_____________41________________23\n	124 -- 130	29 -- 35	-10.2961 kcal/mol	2.85244 kcal/mol	0.998368 kcal/mol	118 136@LB@ \| \|@LB@5'-CUG...AAACC C UUAGC...GUU-3'@LB@ CGUUUUUUUGUUG UAU@LB@ \|:\|\|\|\|\|\|\|:\|\|: \|\|\|@LB@ GUAAAAAAAUAAU AUA@LB@3'-UUU...UUAAA A CGGCA...ACA-5'@LB@ \| \|@LB@ 41 23@LB@@LB@@LB@Energy -6.45 kcal/mol@LB@Hybridization Energy -10.2961 kcal/mol@LB@Unfolding Energy - mRNA 2.85244 kcal/mol@LB@Unfolding Energy - sRNA 0.998368 kcal/mol@LB@@LB@Position - mRNA 119 -- 135@LB@Position - sRNA 24 -- 40@LB@Position Seed - mRNA 124 -- 130@LB@Position Seed - sRNA 29 -- 35@LB@
31	0.005902	0.6127	b1951	rcsA	-8.09	0.100100	163 -- 178	37 -- 51	transcriptional regulator of colanic acid capsular biosynthesis	_____________162______________179\n_____________\|________________\|\n5'-GUU...CAGGU__________A_____ACGCA...UUG-3'\n______________AAGGGGAAUU_UCGUU\n______________\|\|\|:\|\|\|\|\|\|_\|\|:\|\|\n______________UUCUCCUUAA_AGUAA\n3'-UUU...GCAGU________________AAAAA...ACA-5'\n_____________\|________________\|\n_____________52_______________36\n	166 -- 172	42 -- 48	-14.3412 kcal/mol	5.18535 kcal/mol	1.07049 kcal/mol	162 179@LB@ \| \|@LB@5'-GUU...CAGGU A ACGCA...UUG-3'@LB@ AAGGGGAAUU UCGUU@LB@ \|\|\|:\|\|\|\|\|\| \|\|:\|\|@LB@ UUCUCCUUAA AGUAA@LB@3'-UUU...GCAGU AAAAA...ACA-5'@LB@ \| \|@LB@ 52 36@LB@@LB@@LB@Energy -8.09 kcal/mol@LB@Hybridization Energy -14.3412 kcal/mol@LB@Unfolding Energy - mRNA 5.18535 kcal/mol@LB@Unfolding Energy - sRNA 1.07049 kcal/mol@LB@@LB@Position - mRNA 163 -- 178@LB@Position - sRNA 37 -- 51@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 42 -- 48@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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