Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
14	0.003078	0.593	b0886	cydC	-10.90	0.026971	183 -- 199	39 -- 56	glutathione/cysteine ABC transporter export permease/ATPase	_____________182________________200\n_____________\|__________________\|\n5'-GCG...CCCAU_____________A____AAUGC...UCG-3'\n______________CGUC_AGGAGGAG_UUUA\n______________\|\|\|\|_\|:\|\|\|\|\|:_\|\|:\|\n______________GCAG_UUCUCCUU_AAGU\n3'-UUU...ACCGG____U________A____AAAAA...ACA-5'\n_____________\|__________________\|\n_____________57_________________38\n	188 -- 194	44 -- 50	-16.2318 kcal/mol	4.17801 kcal/mol	1.15786 kcal/mol	182 200@LB@ \| \|@LB@5'-GCG...CCCAU A AAUGC...UCG-3'@LB@ CGUC AGGAGGAG UUUA@LB@ \|\|\|\| \|:\|\|\|\|\|: \|\|:\|@LB@ GCAG UUCUCCUU AAGU@LB@3'-UUU...ACCGG U A AAAAA...ACA-5'@LB@ \| \|@LB@ 57 38@LB@@LB@@LB@Energy -10.90 kcal/mol@LB@Hybridization Energy -16.2318 kcal/mol@LB@Unfolding Energy - mRNA 4.17801 kcal/mol@LB@Unfolding Energy - sRNA 1.15786 kcal/mol@LB@@LB@Position - mRNA 183 -- 199@LB@Position - sRNA 39 -- 56@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 44 -- 50@LB@
181	0.03769	0.6177	b0912	ihfB	-7.34	0.140491	192 -- 199	43 -- 50	integration host factor (IHF) DNA-binding protein beta subunit	_____________191______200\n_____________\|________\|\n5'-ACG...GAACC________CAUGA...UGG-3'\n______________GGAGGAAU\n______________:\|\|\|\|\|\|\|\n______________UCUCCUUA\n3'-UUU...CAGUU________AAGUA...ACA-5'\n_____________\|________\|\n_____________51_______42\n	193 -- 199	43 -- 49	-10.3812 kcal/mol	2.20002 kcal/mol	0.845564 kcal/mol	191 200@LB@ \| \|@LB@5'-ACG...GAACC CAUGA...UGG-3'@LB@ GGAGGAAU@LB@ :\|\|\|\|\|\|\|@LB@ UCUCCUUA@LB@3'-UUU...CAGUU AAGUA...ACA-5'@LB@ \| \|@LB@ 51 42@LB@@LB@@LB@Energy -7.34 kcal/mol@LB@Hybridization Energy -10.3812 kcal/mol@LB@Unfolding Energy - mRNA 2.20002 kcal/mol@LB@Unfolding Energy - sRNA 0.845564 kcal/mol@LB@@LB@Position - mRNA 192 -- 199@LB@Position - sRNA 43 -- 50@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 43 -- 49@LB@
113	0.02257	0.6177	b0915	lpxK	-7.55	0.127690	105 -- 116	46 -- 56	lipid A 4'kinase	_____________104__________117\n_____________\|____________\|\n5'-GAA...GUGGU_________U__UGUCA...GCU-3'\n______________CGUCGAGGA_GG\n______________\|\|\|\|:\|:\|\|_\|\|\n______________GCAGUUUCU_CC\n3'-UUU...ACCGG____________UUAAA...ACA-5'\n_____________\|____________\|\n_____________57___________45\n	107 -- 113	48 -- 54	-12.734 kcal/mol	4.25329 kcal/mol	0.93204 kcal/mol	104 117@LB@ \| \|@LB@5'-GAA...GUGGU U UGUCA...GCU-3'@LB@ CGUCGAGGA GG@LB@ \|\|\|\|:\|:\|\| \|\|@LB@ GCAGUUUCU CC@LB@3'-UUU...ACCGG UUAAA...ACA-5'@LB@ \| \|@LB@ 57 45@LB@@LB@@LB@Energy -7.55 kcal/mol@LB@Hybridization Energy -12.734 kcal/mol@LB@Unfolding Energy - mRNA 4.25329 kcal/mol@LB@Unfolding Energy - sRNA 0.93204 kcal/mol@LB@@LB@Position - mRNA 105 -- 116@LB@Position - sRNA 46 -- 56@LB@Position Seed - mRNA 107 -- 113@LB@Position Seed - sRNA 48 -- 54@LB@
178	0.03627	0.6177	b0924	mukB	-9.59	0.049840	186 -- 195	44 -- 53	chromosome condensin MukBEF ATPase and DNA-binding subunit	_____________185________196\n_____________\|__________\|\n5'-UGG...GAUAG__________GAAUA...CUG-3'\n______________CGGAGAGGAA\n______________\|::\|\|\|\|\|\|\|\n______________GUUUCUCCUU\n3'-UUU...GGGCA__________AAAGU...ACA-5'\n_____________\|__________\|\n_____________54_________43\n	189 -- 195	44 -- 50	-13.8471 kcal/mol	3.32824 kcal/mol	0.927997 kcal/mol	185 196@LB@ \| \|@LB@5'-UGG...GAUAG GAAUA...CUG-3'@LB@ CGGAGAGGAA@LB@ \|::\|\|\|\|\|\|\|@LB@ GUUUCUCCUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -13.8471 kcal/mol@LB@Unfolding Energy - mRNA 3.32824 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 186 -- 195@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 44 -- 50@LB@
16	0.003381	0.593	b0956	matP	-12.11	0.015217	269 -- 280	42 -- 53	Ter macrodomain organizer matS-binding protein	_____________268__________281\n_____________\|____________\|\n5'-UUU...CAUCG____________AAUCA...AAG-3'\n______________CGAAGGGGAGUU\n______________\|:\|\|\|:\|\|\|:\|\|\n______________GUUUCUCCUUAA\n3'-UUU...GGGCA____________AGUAA...ACA-5'\n_____________\|____________\|\n_____________54___________41\n	274 -- 280	42 -- 48	-15.3716 kcal/mol	2.33274 kcal/mol	0.933904 kcal/mol	268 281@LB@ \| \|@LB@5'-UUU...CAUCG AAUCA...AAG-3'@LB@ CGAAGGGGAGUU@LB@ \|:\|\|\|:\|\|\|:\|\|@LB@ GUUUCUCCUUAA@LB@3'-UUU...GGGCA AGUAA...ACA-5'@LB@ \| \|@LB@ 54 41@LB@@LB@@LB@Energy -12.11 kcal/mol@LB@Hybridization Energy -15.3716 kcal/mol@LB@Unfolding Energy - mRNA 2.33274 kcal/mol@LB@Unfolding Energy - sRNA 0.933904 kcal/mol@LB@@LB@Position - mRNA 269 -- 280@LB@Position - sRNA 42 -- 53@LB@Position Seed - mRNA 274 -- 280@LB@Position Seed - sRNA 42 -- 48@LB@
155	0.03087	0.6177	b1003	yccJ	-9.03	0.064630	130 -- 145	43 -- 56	uncharacterized protein	_____________129______________146\n_____________\|________________\|\n5'-CCU...UUGCU_____UC_________AUGUC...GGU-3'\n______________CGUUA__AAGGGGAAU\n______________\|\|\|:\|__\|\|\|:\|\|\|\|\|\n______________GCAGU__UUCUCCUUA\n3'-UUU...ACCGG________________AAGUA...ACA-5'\n_____________\|________________\|\n_____________57_______________42\n	139 -- 145	43 -- 49	-13.8314 kcal/mol	3.84343 kcal/mol	0.953958 kcal/mol	129 146@LB@ \| \|@LB@5'-CCU...UUGCU UC AUGUC...GGU-3'@LB@ CGUUA AAGGGGAAU@LB@ \|\|\|:\| \|\|\|:\|\|\|\|\|@LB@ GCAGU UUCUCCUUA@LB@3'-UUU...ACCGG AAGUA...ACA-5'@LB@ \| \|@LB@ 57 42@LB@@LB@@LB@Energy -9.03 kcal/mol@LB@Hybridization Energy -13.8314 kcal/mol@LB@Unfolding Energy - mRNA 3.84343 kcal/mol@LB@Unfolding Energy - sRNA 0.953958 kcal/mol@LB@@LB@Position - mRNA 130 -- 145@LB@Position - sRNA 43 -- 56@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 43 -- 49@LB@
4	0.0002658	0.2138	b1040	csgD	-11.06	0.024985	185 -- 199	38 -- 52	csgBAC operon transcriptional regulator	_____________184_____________200\n_____________\|_______________\|\n5'-UUA...UAAAA____C__________CAUGU...CGC-3'\n______________AAAG_GGGGUUUCAU\n______________\|\|\|\|_\|\|::\|\|\|\|\|\|\n______________UUUC_CCUUAAAGUA\n3'-UUU...GGCAG____U__________AAAAA...ACA-5'\n_____________\|_______________\|\n_____________53______________37\n	193 -- 199	38 -- 44	-14.3947 kcal/mol	2.26766 kcal/mol	1.0693 kcal/mol	184 200@LB@ \| \|@LB@5'-UUA...UAAAA C CAUGU...CGC-3'@LB@ AAAG GGGGUUUCAU@LB@ \|\|\|\| \|\|::\|\|\|\|\|\|@LB@ UUUC CCUUAAAGUA@LB@3'-UUU...GGCAG U AAAAA...ACA-5'@LB@ \| \|@LB@ 53 37@LB@@LB@@LB@Energy -11.06 kcal/mol@LB@Hybridization Energy -14.3947 kcal/mol@LB@Unfolding Energy - mRNA 2.26766 kcal/mol@LB@Unfolding Energy - sRNA 1.0693 kcal/mol@LB@@LB@Position - mRNA 185 -- 199@LB@Position - sRNA 38 -- 52@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 38 -- 44@LB@
8	0.001474	0.5928	b1048	opgG	-11.67	0.018726	159 -- 168	44 -- 53	OPG biosynthetic periplasmic beta-1 6 branching glycosyltransferase	_____________158________169\n_____________\|__________\|\n5'-ACA...GCACA__________AGUGC...AAU-3'\n______________CAAAGGGGGA\n______________\|\|\|\|\|:\|\|:\|\n______________GUUUCUCCUU\n3'-UUU...GGGCA__________AAAGU...ACA-5'\n_____________\|__________\|\n_____________54_________43\n	162 -- 168	44 -- 50	-12.9473 kcal/mol	0.352192 kcal/mol	0.927997 kcal/mol	158 169@LB@ \| \|@LB@5'-ACA...GCACA AGUGC...AAU-3'@LB@ CAAAGGGGGA@LB@ \|\|\|\|\|:\|\|:\|@LB@ GUUUCUCCUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -11.67 kcal/mol@LB@Hybridization Energy -12.9473 kcal/mol@LB@Unfolding Energy - mRNA 0.352192 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 159 -- 168@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 44 -- 50@LB@
198	0.04148	0.6177	b1067	yceH	-6.24	0.226874	185 -- 202	38 -- 56	UPF0502 family protein	_____________184_________________203\n_____________\|___________________\|\n5'-GCA...CCGGC__C___________C____GAAAU...GUG-3'\n______________CG_UAAGGAG_AAU_UCAU\n______________\|\|_:\|\|:\|\|\|_\|\|\|_\|\|\|\|\n______________GC_GUUUCUC_UUA_AGUA\n3'-UUU...ACCGG__A_______C___A____AAAAA...ACA-5'\n_____________\|___________________\|\n_____________57__________________37\n	188 -- 194	47 -- 53	-10.8881 kcal/mol	3.47135 kcal/mol	1.17918 kcal/mol	184 203@LB@ \| \|@LB@5'-GCA...CCGGC C C GAAAU...GUG-3'@LB@ CG UAAGGAG AAU UCAU@LB@ \|\| :\|\|:\|\|\| \|\|\| \|\|\|\|@LB@ GC GUUUCUC UUA AGUA@LB@3'-UUU...ACCGG A C A AAAAA...ACA-5'@LB@ \| \|@LB@ 57 37@LB@@LB@@LB@Energy -6.24 kcal/mol@LB@Hybridization Energy -10.8881 kcal/mol@LB@Unfolding Energy - mRNA 3.47135 kcal/mol@LB@Unfolding Energy - sRNA 1.17918 kcal/mol@LB@@LB@Position - mRNA 185 -- 202@LB@Position - sRNA 38 -- 56@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 47 -- 53@LB@
25	0.004737	0.5992	b1093	fabG	-11.25	0.022809	188 -- 202	38 -- 52	3-oxoacyl-[acyl-carrier-protein] reductase	_____________187_____________203\n_____________\|_______________\|\n5'-UAC...CUUUA_________AA____GAAUU...UUG-3'\n______________AAAGAGGAA__UCAU\n______________\|\|\|\|\|\|\|\|\|__\|\|\|\|\n______________UUUCUCCUU__AGUA\n3'-UUU...GGCAG_________AA____AAAAA...ACA-5'\n_____________\|_______________\|\n_____________53______________37\n	190 -- 196	44 -- 50	-14.2885 kcal/mol	1.96871 kcal/mol	1.0693 kcal/mol	187 203@LB@ \| \|@LB@5'-UAC...CUUUA AA GAAUU...UUG-3'@LB@ AAAGAGGAA UCAU@LB@ \|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ UUUCUCCUU AGUA@LB@3'-UUU...GGCAG AA AAAAA...ACA-5'@LB@ \| \|@LB@ 53 37@LB@@LB@@LB@Energy -11.25 kcal/mol@LB@Hybridization Energy -14.2885 kcal/mol@LB@Unfolding Energy - mRNA 1.96871 kcal/mol@LB@Unfolding Energy - sRNA 1.0693 kcal/mol@LB@@LB@Position - mRNA 188 -- 202@LB@Position - sRNA 38 -- 52@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 44 -- 50@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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