Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
63	0.003338	0.1645	b2671	ygaC	-8.88	0.034356	231 -- 246	25 -- 40	uncharacterized protein	_____________230______________247\n_____________\|________________\|\n5'-UAU...CGCGC____________G___GUUUU...AAA-3'\n______________GUACUUGAAAAA_UCG\n______________\|:\|\|\|\|:\|\|\|\|\|_\|\|\|\n______________CGUGAAUUUUUU_AGC\n3'-UUU...UUCUU____________A___AAUGU...CAA-5'\n_____________\|________________\|\n_____________41_______________24\n	231 -- 237	34 -- 40	-15.9773 kcal/mol	5.44668 kcal/mol	1.64821 kcal/mol	230 247@LB@ \| \|@LB@5'-UAU...CGCGC G GUUUU...AAA-3'@LB@ GUACUUGAAAAA UCG@LB@ \|:\|\|\|\|:\|\|\|\|\| \|\|\|@LB@ CGUGAAUUUUUU AGC@LB@3'-UUU...UUCUU A AAUGU...CAA-5'@LB@ \| \|@LB@ 41 24@LB@@LB@@LB@Energy -8.88 kcal/mol@LB@Hybridization Energy -15.9773 kcal/mol@LB@Unfolding Energy - mRNA 5.44668 kcal/mol@LB@Unfolding Energy - sRNA 1.64821 kcal/mol@LB@@LB@Position - mRNA 231 -- 246@LB@Position - sRNA 25 -- 40@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 34 -- 40@LB@
90	0.006331	0.2266	b1710	btuE	-7.57	0.078405	256 -- 270	25 -- 39	glutathione peroxidase	_____________255_____________271\n_____________\|_______________\|\n5'-GCA...GACCA____G__________CCGGU...UCG-3'\n______________CGCU_GAGAAGUUCG\n______________\|:\|\|_:\|:\|\|:\|\|\|\|\n______________GUGA_UUUUUUAAGC\n3'-UUU...UCUUC____A__________AAUGU...CAA-5'\n_____________\|_______________\|\n_____________40______________24\n	264 -- 270	25 -- 31	-13.949 kcal/mol	4.72897 kcal/mol	1.64678 kcal/mol	255 271@LB@ \| \|@LB@5'-GCA...GACCA G CCGGU...UCG-3'@LB@ CGCU GAGAAGUUCG@LB@ \|:\|\| :\|:\|\|:\|\|\|\|@LB@ GUGA UUUUUUAAGC@LB@3'-UUU...UCUUC A AAUGU...CAA-5'@LB@ \| \|@LB@ 40 24@LB@@LB@@LB@Energy -7.57 kcal/mol@LB@Hybridization Energy -13.949 kcal/mol@LB@Unfolding Energy - mRNA 4.72897 kcal/mol@LB@Unfolding Energy - sRNA 1.64678 kcal/mol@LB@@LB@Position - mRNA 256 -- 270@LB@Position - sRNA 25 -- 39@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 25 -- 31@LB@
93	0.006966	0.2379	b0436	tig	-6.81	0.123164	173 -- 190	25 -- 42	peptidyl-prolyl cis/trans isomerase (trigger factor)	_____________172________________191\n_____________\|__________________\|\n5'-UAC...UGACC_______GU__UUU____AGGUA...UCA-3'\n______________GAGCACU__GA___UUUG\n______________:\|\|\|\|\|\|__:\|___\|\|:\|\n______________UUCGUGA__UU___AAGC\n3'-UUU...CGUUC_______AU__UUU____AAUGU...CAA-5'\n_____________\|__________________\|\n_____________43_________________24\n	173 -- 179	36 -- 42	-10.673 kcal/mol	2.21437 kcal/mol	1.64855 kcal/mol	172 191@LB@ \| \|@LB@5'-UAC...UGACC GU UUU AGGUA...UCA-3'@LB@ GAGCACU GA UUUG@LB@ :\|\|\|\|\|\| :\| \|\|:\|@LB@ UUCGUGA UU AAGC@LB@3'-UUU...CGUUC AU UUU AAUGU...CAA-5'@LB@ \| \|@LB@ 43 24@LB@@LB@@LB@Energy -6.81 kcal/mol@LB@Hybridization Energy -10.673 kcal/mol@LB@Unfolding Energy - mRNA 2.21437 kcal/mol@LB@Unfolding Energy - sRNA 1.64855 kcal/mol@LB@@LB@Position - mRNA 173 -- 190@LB@Position - sRNA 25 -- 42@LB@Position Seed - mRNA 173 -- 179@LB@Position Seed - sRNA 36 -- 42@LB@
104	0.008606	0.2665	b0956	matP	-4.56	0.392859	215 -- 228	25 -- 38	Ter macrodomain organizer matS-binding protein	_____________214____________229\n_____________\|______________\|\n5'-UUU...CAACA_________UC___AAAGC...AAG-3'\n______________ACUUGAAAA__UUG\n______________\|\|\|\|:\|\|\|\|__\|:\|\n______________UGAAUUUUU__AGC\n3'-UUU...CUUCG_________UA___AAUGU...CAA-5'\n_____________\|______________\|\n_____________39_____________24\n	216 -- 222	31 -- 37	-8.38693 kcal/mol	2.39574 kcal/mol	1.4343 kcal/mol	214 229@LB@ \| \|@LB@5'-UUU...CAACA UC AAAGC...AAG-3'@LB@ ACUUGAAAA UUG@LB@ \|\|\|\|:\|\|\|\| \|:\|@LB@ UGAAUUUUU AGC@LB@3'-UUU...CUUCG UA AAUGU...CAA-5'@LB@ \| \|@LB@ 39 24@LB@@LB@@LB@Energy -4.56 kcal/mol@LB@Hybridization Energy -8.38693 kcal/mol@LB@Unfolding Energy - mRNA 2.39574 kcal/mol@LB@Unfolding Energy - sRNA 1.4343 kcal/mol@LB@@LB@Position - mRNA 215 -- 228@LB@Position - sRNA 25 -- 38@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 31 -- 37@LB@
115	0.009659	0.2693	b0155	clcA	-5.80	0.214551	108 -- 124	25 -- 40	H(+)/Cl(-) exchange transporter	_____________107_______________125\n_____________\|_________________\|\n5'-AGU...UUUGU__________C___A__CUUUG...UAG-3'\n______________GCAUUUGAAA_AUU_CG\n______________\|\|\|:\|\|:\|\|\|_\|\|\|_\|\|\n______________CGUGAAUUUU_UAA_GC\n3'-UUU...UUCUU__________U______AAUGU...CAA-5'\n_____________\|_________________\|\n_____________41________________24\n	109 -- 115	33 -- 39	-11.0899 kcal/mol	3.63771 kcal/mol	1.64821 kcal/mol	107 125@LB@ \| \|@LB@5'-AGU...UUUGU C A CUUUG...UAG-3'@LB@ GCAUUUGAAA AUU CG@LB@ \|\|\|:\|\|:\|\|\| \|\|\| \|\|@LB@ CGUGAAUUUU UAA GC@LB@3'-UUU...UUCUU U AAUGU...CAA-5'@LB@ \| \|@LB@ 41 24@LB@@LB@@LB@Energy -5.80 kcal/mol@LB@Hybridization Energy -11.0899 kcal/mol@LB@Unfolding Energy - mRNA 3.63771 kcal/mol@LB@Unfolding Energy - sRNA 1.64821 kcal/mol@LB@@LB@Position - mRNA 108 -- 124@LB@Position - sRNA 25 -- 40@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 33 -- 39@LB@
125	0.01132	0.2916	b4152	frdC	-8.74	0.037729	137 -- 154	25 -- 41	fumarate reductase (anaerobic) membrane anchor subunit	_____________136________________155\n_____________\|__________________\|\n5'-AGA...AGCAG____AAG___________AAAGA...CGG-3'\n______________GGCA___UAGAAAGUUCG\n______________:\|\|\|___\|\|:\|\|\|:\|\|\|\|\n______________UCGU___AUUUUUUAAGC\n3'-UUU...GUUCU____GA____________AAUGU...CAA-5'\n_____________\|__________________\|\n_____________42_________________24\n	148 -- 154	25 -- 31	-13.3883 kcal/mol	3.00162 kcal/mol	1.64839 kcal/mol	136 155@LB@ \| \|@LB@5'-AGA...AGCAG AAG AAAGA...CGG-3'@LB@ GGCA UAGAAAGUUCG@LB@ :\|\|\| \|\|:\|\|\|:\|\|\|\|@LB@ UCGU AUUUUUUAAGC@LB@3'-UUU...GUUCU GA AAUGU...CAA-5'@LB@ \| \|@LB@ 42 24@LB@@LB@@LB@Energy -8.74 kcal/mol@LB@Hybridization Energy -13.3883 kcal/mol@LB@Unfolding Energy - mRNA 3.00162 kcal/mol@LB@Unfolding Energy - sRNA 1.64839 kcal/mol@LB@@LB@Position - mRNA 137 -- 154@LB@Position - sRNA 25 -- 41@LB@Position Seed - mRNA 148 -- 154@LB@Position Seed - sRNA 25 -- 31@LB@
164	0.01824	0.3566	b1854	pykA	-7.33	0.090768	1 -- 31	25 -- 60	pyruvate kinase II	______________1___________________________________32\n______________\|___________________________________\|\n___________5'-_____GG__CA_____________________A___CGUUU...ACG-3'\n______________GAAGC__GU______AAGAAGCGCU_GAAGGA_UCG\n______________\|\|\|:\|__\|:______\|\|\|\|\|\|\|:\|\|_:\|\|::\|_\|\|\|\n______________CUUUG__CG______UUCUUCGUGA_UUUUUU_AGC\n3'-UUU...CCCUA_____AA__AAUUCG__________A______A___AAUGU...CAA-5'\n_____________\|____________________________________\|\n_____________61___________________________________24\n	15 -- 21	36 -- 42	-23.5606 kcal/mol	9.95433 kcal/mol	6.27609 kcal/mol	1 32@LB@ \| \|@LB@ 5'- GG CA A CGUUU...ACG-3'@LB@ GAAGC GU AAGAAGCGCU GAAGGA UCG@LB@ \|\|\|:\| \|: \|\|\|\|\|\|\|:\|\| :\|\|::\| \|\|\|@LB@ CUUUG CG UUCUUCGUGA UUUUUU AGC@LB@3'-UUU...CCCUA AA AAUUCG A A AAUGU...CAA-5'@LB@ \| \|@LB@ 61 24@LB@@LB@@LB@Energy -7.33 kcal/mol@LB@Hybridization Energy -23.5606 kcal/mol@LB@Unfolding Energy - mRNA 9.95433 kcal/mol@LB@Unfolding Energy - sRNA 6.27609 kcal/mol@LB@@LB@Position - mRNA 1 -- 31@LB@Position - sRNA 25 -- 60@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 36 -- 42@LB@
165	0.01827	0.3566	b2937	speB	-7.30	0.092452	5 -- 22	25 -- 41	agmatinase	_____________4__________________23\n_____________\|__________________\|\n_______5'-CAAC___UC_____________AGGCA...CAG-3'\n______________AGU__CUUGAAGAGUUCG\n______________\|\|:__\|\|\|:\|\|:\|:\|\|\|\|\n______________UCG__GAAUUUUUUAAGC\n3'-UUU...GUUCU___U______________AAUGU...CAA-5'\n_____________\|__________________\|\n_____________42_________________24\n	16 -- 22	25 -- 31	-14.9284 kcal/mol	5.98061 kcal/mol	1.64839 kcal/mol	4 23@LB@ \| \|@LB@ 5'-CAAC UC AGGCA...CAG-3'@LB@ AGU CUUGAAGAGUUCG@LB@ \|\|: \|\|\|:\|\|:\|:\|\|\|\|@LB@ UCG GAAUUUUUUAAGC@LB@3'-UUU...GUUCU U AAUGU...CAA-5'@LB@ \| \|@LB@ 42 24@LB@@LB@@LB@Energy -7.30 kcal/mol@LB@Hybridization Energy -14.9284 kcal/mol@LB@Unfolding Energy - mRNA 5.98061 kcal/mol@LB@Unfolding Energy - sRNA 1.64839 kcal/mol@LB@@LB@Position - mRNA 5 -- 22@LB@Position - sRNA 25 -- 41@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 25 -- 31@LB@
168	0.01885	0.3614	b1792	yeaO	-5.97	0.195997	133 -- 142	25 -- 34	DUF488 family protein	_____________132________143\n_____________\|__________\|\n5'-UGU...AGACA__________CUAAU...CCG-3'\n______________AAGAAAUUCG\n______________\|\|:\|\|\|\|\|\|\|\n______________UUUUUUAAGC\n3'-UUU...GUGAA__________AAUGU...CAA-5'\n_____________\|__________\|\n_____________35_________24\n	136 -- 142	25 -- 31	-8.43117 kcal/mol	1.28773 kcal/mol	1.16883 kcal/mol	132 143@LB@ \| \|@LB@5'-UGU...AGACA CUAAU...CCG-3'@LB@ AAGAAAUUCG@LB@ \|\|:\|\|\|\|\|\|\|@LB@ UUUUUUAAGC@LB@3'-UUU...GUGAA AAUGU...CAA-5'@LB@ \| \|@LB@ 35 24@LB@@LB@@LB@Energy -5.97 kcal/mol@LB@Hybridization Energy -8.43117 kcal/mol@LB@Unfolding Energy - mRNA 1.28773 kcal/mol@LB@Unfolding Energy - sRNA 1.16883 kcal/mol@LB@@LB@Position - mRNA 133 -- 142@LB@Position - sRNA 25 -- 34@LB@Position Seed - mRNA 136 -- 142@LB@Position Seed - sRNA 25 -- 31@LB@
169	0.01902	0.3625	b0858	ybjO	-5.59	0.239654	140 -- 156	25 -- 43	DUF2593 family inner membrane protein	_____________139__________________157\n_____________\|____________________\|\n5'-AUG...UGGCU____A_______________CCAAC...UUA-3'\n______________GAAG_CACU___AAAAUUUG\n______________\|\|\|\|_\|\|\|\|___\|\|\|\|\|\|:\|\n______________CUUC_GUGA___UUUUAAGC\n3'-UUU...UCGUU_________AUU________AAUGU...CAA-5'\n_____________\|____________________\|\n_____________44___________________24\n	150 -- 156	25 -- 31	-11.6219 kcal/mol	2.26879 kcal/mol	3.76226 kcal/mol	139 157@LB@ \| \|@LB@5'-AUG...UGGCU A CCAAC...UUA-3'@LB@ GAAG CACU AAAAUUUG@LB@ \|\|\|\| \|\|\|\| \|\|\|\|\|\|:\|@LB@ CUUC GUGA UUUUAAGC@LB@3'-UUU...UCGUU AUU AAUGU...CAA-5'@LB@ \| \|@LB@ 44 24@LB@@LB@@LB@Energy -5.59 kcal/mol@LB@Hybridization Energy -11.6219 kcal/mol@LB@Unfolding Energy - mRNA 2.26879 kcal/mol@LB@Unfolding Energy - sRNA 3.76226 kcal/mol@LB@@LB@Position - mRNA 140 -- 156@LB@Position - sRNA 25 -- 43@LB@Position Seed - mRNA 150 -- 156@LB@Position Seed - sRNA 25 -- 31@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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