Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
151	0.0157	0.3349	b0586	entF	-8.26	0.051334	189 -- 199	37 -- 47	enterobactin synthase multienzyme complex component ATP-dependent	_____________188_________200\n_____________\|___________\|\n5'-CCC...CAGUU___________AAUGA...UUG-3'\n______________GCAGGAGGCAC\n______________\|\|\|:\|\|:\|\|\|\|\n______________CGUUCUUCGUG\n3'-UUU...GAAUU___________AAUUU...CAA-5'\n_____________\|___________\|\n_____________48__________36\n	193 -- 199	37 -- 43	-17.6877 kcal/mol	4.1913 kcal/mol	5.23944 kcal/mol	188 200@LB@ \| \|@LB@5'-CCC...CAGUU AAUGA...UUG-3'@LB@ GCAGGAGGCAC@LB@ \|\|\|:\|\|:\|\|\|\|@LB@ CGUUCUUCGUG@LB@3'-UUU...GAAUU AAUUU...CAA-5'@LB@ \| \|@LB@ 48 36@LB@@LB@@LB@Energy -8.26 kcal/mol@LB@Hybridization Energy -17.6877 kcal/mol@LB@Unfolding Energy - mRNA 4.1913 kcal/mol@LB@Unfolding Energy - sRNA 5.23944 kcal/mol@LB@@LB@Position - mRNA 189 -- 199@LB@Position - sRNA 37 -- 47@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 37 -- 43@LB@
153	0.016	0.3359	b2167	fruA	-4.10	0.476180	191 -- 199	37 -- 45	fused fructose-specific PTS enzymes: IIBcomponent/IIC components	_____________190_______200\n_____________\|_________\|\n5'-CGC...AGCAG_________AAUGA...AAA-3'\n______________GAGAGGCAU\n______________:\|\|\|:\|\|\|:\n______________UUCUUCGUG\n3'-UUU...AUUCG_________AAUUU...CAA-5'\n_____________\|_________\|\n_____________46________36\n	193 -- 199	37 -- 43	-10.9543 kcal/mol	1.97714 kcal/mol	4.8783 kcal/mol	190 200@LB@ \| \|@LB@5'-CGC...AGCAG AAUGA...AAA-3'@LB@ GAGAGGCAU@LB@ :\|\|\|:\|\|\|:@LB@ UUCUUCGUG@LB@3'-UUU...AUUCG AAUUU...CAA-5'@LB@ \| \|@LB@ 46 36@LB@@LB@@LB@Energy -4.10 kcal/mol@LB@Hybridization Energy -10.9543 kcal/mol@LB@Unfolding Energy - mRNA 1.97714 kcal/mol@LB@Unfolding Energy - sRNA 4.8783 kcal/mol@LB@@LB@Position - mRNA 191 -- 199@LB@Position - sRNA 37 -- 45@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 37 -- 43@LB@
167	0.01879	0.3614	b4383	deoB	-8.94	0.033088	90 -- 118	37 -- 59	phosphopentomutase	_____________89____________________________119\n_____________\|_____________________________\|\n5'-AGG...CAAUU________CGAUA_____CC__A______ACCAA...AUA-3'\n______________AAACUUGC_____AAGCA__GG_AAGCAC\n______________\|\|\|\|\|\|\|\|_____\|\|\|\|\|__:\|_\|\|\|\|\|\|\n______________UUUGAACG_____UUCGU__UC_UUCGUG\n3'-UUU...CCUAC________AA___________________AAUUU...CAA-5'\n_____________\|_____________________________\|\n_____________60____________________________36\n	91 -- 97	52 -- 58	-21.3309 kcal/mol	6.14475 kcal/mol	6.24627 kcal/mol	89 119@LB@ \| \|@LB@5'-AGG...CAAUU CGAUA CC A ACCAA...AUA-3'@LB@ AAACUUGC AAGCA GG AAGCAC@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\| :\| \|\|\|\|\|\|@LB@ UUUGAACG UUCGU UC UUCGUG@LB@3'-UUU...CCUAC AA AAUUU...CAA-5'@LB@ \| \|@LB@ 60 36@LB@@LB@@LB@Energy -8.94 kcal/mol@LB@Hybridization Energy -21.3309 kcal/mol@LB@Unfolding Energy - mRNA 6.14475 kcal/mol@LB@Unfolding Energy - sRNA 6.24627 kcal/mol@LB@@LB@Position - mRNA 90 -- 118@LB@Position - sRNA 37 -- 59@LB@Position Seed - mRNA 91 -- 97@LB@Position Seed - sRNA 52 -- 58@LB@
177	0.02045	0.3721	b3148	yraN	-6.85	0.120614	263 -- 276	37 -- 54	UPF0102 family protein	_____________262________________277\n_____________\|__________________\|\n5'-GGU...GGCGA__________________AAGCG...GCA-3'\n______________UGC____GUGGGAAGCAC\n______________\|\|\|____\|:::\|\|\|\|\|\|\|\n______________ACG____CGUUCUUCGUG\n3'-UUU...UUUGA___AAUU___________AAUUU...CAA-5'\n_____________\|__________________\|\n_____________55_________________36\n	270 -- 276	37 -- 43	-17.1481 kcal/mol	4.14332 kcal/mol	6.1584 kcal/mol	262 277@LB@ \| \|@LB@5'-GGU...GGCGA AAGCG...GCA-3'@LB@ UGC GUGGGAAGCAC@LB@ \|\|\| \|:::\|\|\|\|\|\|\|@LB@ ACG CGUUCUUCGUG@LB@3'-UUU...UUUGA AAUU AAUUU...CAA-5'@LB@ \| \|@LB@ 55 36@LB@@LB@@LB@Energy -6.85 kcal/mol@LB@Hybridization Energy -17.1481 kcal/mol@LB@Unfolding Energy - mRNA 4.14332 kcal/mol@LB@Unfolding Energy - sRNA 6.1584 kcal/mol@LB@@LB@Position - mRNA 263 -- 276@LB@Position - sRNA 37 -- 54@LB@Position Seed - mRNA 270 -- 276@LB@Position Seed - sRNA 37 -- 43@LB@
187	0.02188	0.3768	b3065	rpsU	-8.54	0.042773	188 -- 200	37 -- 49	30S ribosomal subunit protein S21	_____________187___________201\n_____________\|_____________\|\n5'-GAC...AUCAA_____________AUGCC...GUC-3'\n______________AGGUGAGAGGCAC\n______________\|:\|::\|\|\|:\|\|\|\|\n______________UUCGUUCUUCGUG\n3'-UUU...ACGAA_____________AAUUU...CAA-5'\n_____________\|_____________\|\n_____________50____________36\n	194 -- 200	37 -- 43	-18.635 kcal/mol	4.05763 kcal/mol	6.03369 kcal/mol	187 201@LB@ \| \|@LB@5'-GAC...AUCAA AUGCC...GUC-3'@LB@ AGGUGAGAGGCAC@LB@ \|:\|::\|\|\|:\|\|\|\|@LB@ UUCGUUCUUCGUG@LB@3'-UUU...ACGAA AAUUU...CAA-5'@LB@ \| \|@LB@ 50 36@LB@@LB@@LB@Energy -8.54 kcal/mol@LB@Hybridization Energy -18.635 kcal/mol@LB@Unfolding Energy - mRNA 4.05763 kcal/mol@LB@Unfolding Energy - sRNA 6.03369 kcal/mol@LB@@LB@Position - mRNA 188 -- 200@LB@Position - sRNA 37 -- 49@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 37 -- 43@LB@
188	0.02199	0.3768	b3774	ilvC	-7.43	0.085257	172 -- 181	37 -- 46	ketol-acid reductoisomerase NAD(P)-binding	_____________171________182\n_____________\|__________\|\n5'-AUG...AACAA__________AACAU...GCU-3'\n______________UAAGAAGCAC\n______________:\|\|\|\|\|\|\|\|\|\n______________GUUCUUCGUG\n3'-UUU...AAUUC__________AAUUU...CAA-5'\n_____________\|__________\|\n_____________47_________36\n	175 -- 181	37 -- 43	-13.663 kcal/mol	1.01181 kcal/mol	5.21654 kcal/mol	171 182@LB@ \| \|@LB@5'-AUG...AACAA AACAU...GCU-3'@LB@ UAAGAAGCAC@LB@ :\|\|\|\|\|\|\|\|\|@LB@ GUUCUUCGUG@LB@3'-UUU...AAUUC AAUUU...CAA-5'@LB@ \| \|@LB@ 47 36@LB@@LB@@LB@Energy -7.43 kcal/mol@LB@Hybridization Energy -13.663 kcal/mol@LB@Unfolding Energy - mRNA 1.01181 kcal/mol@LB@Unfolding Energy - sRNA 5.21654 kcal/mol@LB@@LB@Position - mRNA 172 -- 181@LB@Position - sRNA 37 -- 46@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 37 -- 43@LB@
30	0.0008402	0.09021	b2792	yqcC	-12.92	0.001907	10 -- 25	38 -- 53	DUF446 family protein	_____________9________________26\n_____________\|________________\|\n5'-AUC...GGGUG__G_____________AUAGU...AAU-3'\n______________GU_UGAGCAGGAAGCA\n______________\|:_\|:\|\|\|\|:\|\|\|\|\|\|\n______________CG_AUUCGUUCUUCGU\n3'-UUU...UUGAA__A_____________GAAUU...CAA-5'\n_____________\|________________\|\n_____________54_______________37\n	19 -- 25	38 -- 44	-21.2799 kcal/mol	2.27149 kcal/mol	6.09301 kcal/mol	9 26@LB@ \| \|@LB@5'-AUC...GGGUG G AUAGU...AAU-3'@LB@ GU UGAGCAGGAAGCA@LB@ \|: \|:\|\|\|\|:\|\|\|\|\|\|@LB@ CG AUUCGUUCUUCGU@LB@3'-UUU...UUGAA A GAAUU...CAA-5'@LB@ \| \|@LB@ 54 37@LB@@LB@@LB@Energy -12.92 kcal/mol@LB@Hybridization Energy -21.2799 kcal/mol@LB@Unfolding Energy - mRNA 2.27149 kcal/mol@LB@Unfolding Energy - sRNA 6.09301 kcal/mol@LB@@LB@Position - mRNA 10 -- 25@LB@Position - sRNA 38 -- 53@LB@Position Seed - mRNA 19 -- 25@LB@Position Seed - sRNA 38 -- 44@LB@
45	0.001912	0.1369	b0080	cra	-5.81	0.213706	183 -- 197	38 -- 52	transcriptional repressor-activator for carbon metabolism	_____________182_____________198\n_____________\|_______________\|\n5'-GGC...UACAU____C__________AUUGU...AAA-3'\n______________UUUA_GCAAGGGGCA\n______________:\|\|\|_\|\|\|\|\|::\|\|\|\n______________GAAU_CGUUCUUCGU\n3'-UUU...UGAAC____U__________GAAUU...CAA-5'\n_____________\|_______________\|\n_____________53______________37\n	191 -- 197	38 -- 44	-16.0644 kcal/mol	4.16124 kcal/mol	6.09169 kcal/mol	182 198@LB@ \| \|@LB@5'-GGC...UACAU C AUUGU...AAA-3'@LB@ UUUA GCAAGGGGCA@LB@ :\|\|\| \|\|\|\|\|::\|\|\|@LB@ GAAU CGUUCUUCGU@LB@3'-UUU...UGAAC U GAAUU...CAA-5'@LB@ \| \|@LB@ 53 37@LB@@LB@@LB@Energy -5.81 kcal/mol@LB@Hybridization Energy -16.0644 kcal/mol@LB@Unfolding Energy - mRNA 4.16124 kcal/mol@LB@Unfolding Energy - sRNA 6.09169 kcal/mol@LB@@LB@Position - mRNA 183 -- 197@LB@Position - sRNA 38 -- 52@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 38 -- 44@LB@
71	0.00365	0.1656	b4478	dgoD	-6.42	0.153775	171 -- 195	38 -- 57	D-galactonate dehydratase	_____________170_______________________196\n_____________\|_________________________\|\n5'-AAA...GCAGC_______A____GUAUC________GUGCA...UGA-3'\n______________AUUUGUU_AGGC_____GAGAGGCA\n______________\|:\|\|\|:\|_\|:\|\|_____:\|\|\|:\|\|\|\n______________UGAACGA_UUCG_____UUCUUCGU\n3'-UUU...UACUU_______A_________________GAAUU...CAA-5'\n_____________\|_________________________\|\n_____________58________________________37\n	189 -- 195	38 -- 44	-17.6382 kcal/mol	5.02573 kcal/mol	6.19395 kcal/mol	170 196@LB@ \| \|@LB@5'-AAA...GCAGC A GUAUC GUGCA...UGA-3'@LB@ AUUUGUU AGGC GAGAGGCA@LB@ \|:\|\|\|:\| \|:\|\| :\|\|\|:\|\|\|@LB@ UGAACGA UUCG UUCUUCGU@LB@3'-UUU...UACUU A GAAUU...CAA-5'@LB@ \| \|@LB@ 58 37@LB@@LB@@LB@Energy -6.42 kcal/mol@LB@Hybridization Energy -17.6382 kcal/mol@LB@Unfolding Energy - mRNA 5.02573 kcal/mol@LB@Unfolding Energy - sRNA 6.19395 kcal/mol@LB@@LB@Position - mRNA 171 -- 195@LB@Position - sRNA 38 -- 57@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 38 -- 44@LB@
72	0.003773	0.1688	b1860	ruvB	-7.81	0.067658	103 -- 111	38 -- 46	ATP-dependent DNA helicase component of RuvABC resolvasome	_____________102_______112\n_____________\|_________\|\n5'-ACG...AACCA_________AGCCG...GUC-3'\n______________CAAGAAGCA\n______________\|\|\|\|\|\|\|\|\|\n______________GUUCUUCGU\n3'-UUU...AAUUC_________GAAUU...CAA-5'\n_____________\|_________\|\n_____________47________37\n	105 -- 111	38 -- 44	-11.4188 kcal/mol	0.978333 kcal/mol	2.62609 kcal/mol	102 112@LB@ \| \|@LB@5'-ACG...AACCA AGCCG...GUC-3'@LB@ CAAGAAGCA@LB@ \|\|\|\|\|\|\|\|\|@LB@ GUUCUUCGU@LB@3'-UUU...AAUUC GAAUU...CAA-5'@LB@ \| \|@LB@ 47 37@LB@@LB@@LB@Energy -7.81 kcal/mol@LB@Hybridization Energy -11.4188 kcal/mol@LB@Unfolding Energy - mRNA 0.978333 kcal/mol@LB@Unfolding Energy - sRNA 2.62609 kcal/mol@LB@@LB@Position - mRNA 103 -- 111@LB@Position - sRNA 38 -- 46@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 38 -- 44@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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