Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
77	0.004637	0.1935	b0407	yajC	-7.39	0.087827	147 -- 158	37 -- 48	SecYEG protein translocase auxillary subunit	_____________146__________159\n_____________\|____________\|\n5'-UCU...UCAGG____________CACCU...UUU-3'\n______________GGCGAGAAGUAC\n______________:\|\|:\|\|\|\|\|:\|\|\n______________UCGUUCUUCGUG\n3'-UUU...CGAAU____________AAUUU...CAA-5'\n_____________\|____________\|\n_____________49___________36\n	152 -- 158	37 -- 43	-17.5904 kcal/mol	4.37033 kcal/mol	5.83483 kcal/mol	146 159@LB@ \| \|@LB@5'-UCU...UCAGG CACCU...UUU-3'@LB@ GGCGAGAAGUAC@LB@ :\|\|:\|\|\|\|\|:\|\|@LB@ UCGUUCUUCGUG@LB@3'-UUU...CGAAU AAUUU...CAA-5'@LB@ \| \|@LB@ 49 36@LB@@LB@@LB@Energy -7.39 kcal/mol@LB@Hybridization Energy -17.5904 kcal/mol@LB@Unfolding Energy - mRNA 4.37033 kcal/mol@LB@Unfolding Energy - sRNA 5.83483 kcal/mol@LB@@LB@Position - mRNA 147 -- 158@LB@Position - sRNA 37 -- 48@LB@Position Seed - mRNA 152 -- 158@LB@Position Seed - sRNA 37 -- 43@LB@
83	0.005546	0.2146	b0221	fadE	-11.24	0.006780	20 -- 32	37 -- 49	acyl coenzyme A dehydrogenase	_____________19____________33\n_____________\|_____________\|\n5'-GUG...GCCAA_____________GGGCA...GGA-3'\n______________AAGCGAGAAGUAC\n______________\|\|\|\|:\|\|\|\|\|:\|\|\n______________UUCGUUCUUCGUG\n3'-UUU...ACGAA_____________AAUUU...CAA-5'\n_____________\|_____________\|\n_____________50____________36\n	26 -- 32	37 -- 43	-19.9972 kcal/mol	2.7283 kcal/mol	6.03369 kcal/mol	19 33@LB@ \| \|@LB@5'-GUG...GCCAA GGGCA...GGA-3'@LB@ AAGCGAGAAGUAC@LB@ \|\|\|\|:\|\|\|\|\|:\|\|@LB@ UUCGUUCUUCGUG@LB@3'-UUU...ACGAA AAUUU...CAA-5'@LB@ \| \|@LB@ 50 36@LB@@LB@@LB@Energy -11.24 kcal/mol@LB@Hybridization Energy -19.9972 kcal/mol@LB@Unfolding Energy - mRNA 2.7283 kcal/mol@LB@Unfolding Energy - sRNA 6.03369 kcal/mol@LB@@LB@Position - mRNA 20 -- 32@LB@Position - sRNA 37 -- 49@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 37 -- 43@LB@
92	0.006692	0.2343	b2421	cysM	-10.79	0.009324	40 -- 63	37 -- 60	cysteine synthase B (O-acetylserine sulfhydrolase B)	_____________39_______________________64\n_____________\|________________________\|\n5'-GCU...GAUGA__________CUC___CA______CUGAU...GCA-3'\n______________GGAACUUGCU___GCA__AAGCGC\n______________\|:\|\|\|\|\|\|\|\|___\|\|\|__\|\|\|\|:\|\n______________CUUUGAACGA___CGU__UUCGUG\n3'-UUU...CCCUA__________AUU___UC______AAUUU...CAA-5'\n_____________\|________________________\|\n_____________61_______________________36\n	43 -- 49	51 -- 57	-24.0401 kcal/mol	7.00094 kcal/mol	6.24651 kcal/mol	39 64@LB@ \| \|@LB@5'-GCU...GAUGA CUC CA CUGAU...GCA-3'@LB@ GGAACUUGCU GCA AAGCGC@LB@ \|:\|\|\|\|\|\|\|\| \|\|\| \|\|\|\|:\|@LB@ CUUUGAACGA CGU UUCGUG@LB@3'-UUU...CCCUA AUU UC AAUUU...CAA-5'@LB@ \| \|@LB@ 61 36@LB@@LB@@LB@Energy -10.79 kcal/mol@LB@Hybridization Energy -24.0401 kcal/mol@LB@Unfolding Energy - mRNA 7.00094 kcal/mol@LB@Unfolding Energy - sRNA 6.24651 kcal/mol@LB@@LB@Position - mRNA 40 -- 63@LB@Position - sRNA 37 -- 60@LB@Position Seed - mRNA 43 -- 49@LB@Position Seed - sRNA 51 -- 57@LB@
95	0.007016	0.2379	b0464	acrR	-8.52	0.043413	219 -- 228	37 -- 46	transcriptional repressor	_____________218________229\n_____________\|__________\|\n5'-AAG...CCAAA__________AAGAA...CGC-3'\n______________CAAGAAGCGC\n______________\|\|\|\|\|\|\|\|:\|\n______________GUUCUUCGUG\n3'-UUU...AAUUC__________AAUUU...CAA-5'\n_____________\|__________\|\n_____________47_________36\n	222 -- 228	37 -- 43	-15.2794 kcal/mol	1.54243 kcal/mol	5.21654 kcal/mol	218 229@LB@ \| \|@LB@5'-AAG...CCAAA AAGAA...CGC-3'@LB@ CAAGAAGCGC@LB@ \|\|\|\|\|\|\|\|:\|@LB@ GUUCUUCGUG@LB@3'-UUU...AAUUC AAUUU...CAA-5'@LB@ \| \|@LB@ 47 36@LB@@LB@@LB@Energy -8.52 kcal/mol@LB@Hybridization Energy -15.2794 kcal/mol@LB@Unfolding Energy - mRNA 1.54243 kcal/mol@LB@Unfolding Energy - sRNA 5.21654 kcal/mol@LB@@LB@Position - mRNA 219 -- 228@LB@Position - sRNA 37 -- 46@LB@Position Seed - mRNA 222 -- 228@LB@Position Seed - sRNA 37 -- 43@LB@
96	0.007122	0.239	b2060	wzc	-5.51	0.249431	285 -- 291	37 -- 43	colanic acid production tyrosine-protein kinase; autokinase; Ugd phosphorylase	_____________284_____292\n_____________\|_______\|\n5'-GAA...UCAUU_______GCUGG...GGG-3'\n______________GAAGCGC\n______________\|\|\|\|\|:\|\n______________CUUCGUG\n3'-UUU...UCGUU_______AAUUU...CAA-5'\n_____________\|_______\|\n_____________44______36\n	285 -- 291	37 -- 43	-9.9751 kcal/mol	0.803081 kcal/mol	3.6595 kcal/mol	284 292@LB@ \| \|@LB@5'-GAA...UCAUU GCUGG...GGG-3'@LB@ GAAGCGC@LB@ \|\|\|\|\|:\|@LB@ CUUCGUG@LB@3'-UUU...UCGUU AAUUU...CAA-5'@LB@ \| \|@LB@ 44 36@LB@@LB@@LB@Energy -5.51 kcal/mol@LB@Hybridization Energy -9.9751 kcal/mol@LB@Unfolding Energy - mRNA 0.803081 kcal/mol@LB@Unfolding Energy - sRNA 3.6595 kcal/mol@LB@@LB@Position - mRNA 285 -- 291@LB@Position - sRNA 37 -- 43@LB@Position Seed - mRNA 285 -- 291@LB@Position Seed - sRNA 37 -- 43@LB@
97	0.007216	0.239	b2021	hisC	-5.42	0.261518	59 -- 66	37 -- 44	histidinol-phosphate aminotransferase	_____________58_______67\n_____________\|________\|\n5'-GUU...UUUCC________AUGAC...GGC-3'\n______________AGAAGCGC\n______________\|\|\|\|\|\|:\|\n______________UCUUCGUG\n3'-UUU...UUCGU________AAUUU...CAA-5'\n_____________\|________\|\n_____________45_______36\n	60 -- 66	37 -- 43	-11.6623 kcal/mol	1.85203 kcal/mol	4.39141 kcal/mol	58 67@LB@ \| \|@LB@5'-GUU...UUUCC AUGAC...GGC-3'@LB@ AGAAGCGC@LB@ \|\|\|\|\|\|:\|@LB@ UCUUCGUG@LB@3'-UUU...UUCGU AAUUU...CAA-5'@LB@ \| \|@LB@ 45 36@LB@@LB@@LB@Energy -5.42 kcal/mol@LB@Hybridization Energy -11.6623 kcal/mol@LB@Unfolding Energy - mRNA 1.85203 kcal/mol@LB@Unfolding Energy - sRNA 4.39141 kcal/mol@LB@@LB@Position - mRNA 59 -- 66@LB@Position - sRNA 37 -- 44@LB@Position Seed - mRNA 60 -- 66@LB@Position Seed - sRNA 37 -- 43@LB@
123	0.0108	0.2828	b2606	rplS	-6.81	0.122900	220 -- 252	37 -- 58	50S ribosomal subunit protein L19	_____________219_______________________________253\n_____________\|_________________________________\|\n5'-AUA...UAAGC______AACAAGAGCAGAUG________C____CUUCC...UUG-3'\n______________AACUUG______________AAGCAGGA_GUAC\n______________\|\|\|\|\|\|______________\|\|\|\|\|:\|\|_\|:\|\|\n______________UUGAAC______________UUCGUUCU_CGUG\n3'-UUU...CUACU______GAA___________________U____AAUUU...CAA-5'\n_____________\|_________________________________\|\n_____________59________________________________36\n	241 -- 247	42 -- 48	-17.5998 kcal/mol	4.54246 kcal/mol	6.24354 kcal/mol	219 253@LB@ \| \|@LB@5'-AUA...UAAGC AACAAGAGCAGAUG C CUUCC...UUG-3'@LB@ AACUUG AAGCAGGA GUAC@LB@ \|\|\|\|\|\| \|\|\|\|\|:\|\| \|:\|\|@LB@ UUGAAC UUCGUUCU CGUG@LB@3'-UUU...CUACU GAA U AAUUU...CAA-5'@LB@ \| \|@LB@ 59 36@LB@@LB@@LB@Energy -6.81 kcal/mol@LB@Hybridization Energy -17.5998 kcal/mol@LB@Unfolding Energy - mRNA 4.54246 kcal/mol@LB@Unfolding Energy - sRNA 6.24354 kcal/mol@LB@@LB@Position - mRNA 220 -- 252@LB@Position - sRNA 37 -- 58@LB@Position Seed - mRNA 241 -- 247@LB@Position Seed - sRNA 42 -- 48@LB@
140	0.01344	0.3093	b0426	yajQ	-3.92	0.510019	240 -- 246	37 -- 43	phage Phi6 host factor ATP/GTP binding protein	_____________239_____247\n_____________\|_______\|\n5'-UAA...UUCAG_______GUAAC...GUA-3'\n______________GAAGCAC\n______________\|\|\|\|\|\|\|\n______________CUUCGUG\n3'-UUU...UCGUU_______AAUUU...CAA-5'\n_____________\|_______\|\n_____________44______36\n	240 -- 246	37 -- 43	-10.1802 kcal/mol	2.59742 kcal/mol	3.6595 kcal/mol	239 247@LB@ \| \|@LB@5'-UAA...UUCAG GUAAC...GUA-3'@LB@ GAAGCAC@LB@ \|\|\|\|\|\|\|@LB@ CUUCGUG@LB@3'-UUU...UCGUU AAUUU...CAA-5'@LB@ \| \|@LB@ 44 36@LB@@LB@@LB@Energy -3.92 kcal/mol@LB@Hybridization Energy -10.1802 kcal/mol@LB@Unfolding Energy - mRNA 2.59742 kcal/mol@LB@Unfolding Energy - sRNA 3.6595 kcal/mol@LB@@LB@Position - mRNA 240 -- 246@LB@Position - sRNA 37 -- 43@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 37 -- 43@LB@
143	0.01412	0.3181	b0642	leuS	-6.82	0.122159	244 -- 266	37 -- 58	leucyl-tRNA synthetase	_____________243_____________________267\n_____________\|_______________________\|\n5'-AAU...AGUAC_____CA_____A__________ACAUU...AGA-3'\n______________AGCUU__UUGGG_UGAGAAGCGC\n______________\|:\|\|\|__\|\|::\|_::\|\|\|\|\|\|:\|\n______________UUGAA__AAUUC_GUUCUUCGUG\n3'-UUU...CUACU_____CG________________AAUUU...CAA-5'\n_____________\|_______________________\|\n_____________59______________________36\n	260 -- 266	37 -- 43	-20.1861 kcal/mol	7.11823 kcal/mol	6.24354 kcal/mol	243 267@LB@ \| \|@LB@5'-AAU...AGUAC CA A ACAUU...AGA-3'@LB@ AGCUU UUGGG UGAGAAGCGC@LB@ \|:\|\|\| \|\|::\| ::\|\|\|\|\|\|:\|@LB@ UUGAA AAUUC GUUCUUCGUG@LB@3'-UUU...CUACU CG AAUUU...CAA-5'@LB@ \| \|@LB@ 59 36@LB@@LB@@LB@Energy -6.82 kcal/mol@LB@Hybridization Energy -20.1861 kcal/mol@LB@Unfolding Energy - mRNA 7.11823 kcal/mol@LB@Unfolding Energy - sRNA 6.24354 kcal/mol@LB@@LB@Position - mRNA 244 -- 266@LB@Position - sRNA 37 -- 58@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 37 -- 43@LB@
150	0.01547	0.3321	b3736	atpF	-5.86	0.208717	262 -- 272	37 -- 47	F0 sector of membrane-bound ATP synthase subunit b	_____________261_________273\n_____________\|___________\|\n5'-AUU...GUUCU___U_______GUAUG...UCG-3'\n______________GCA_GAAGUAC\n______________\|\|\|_\|\|\|\|:\|\|\n______________CGU_CUUCGUG\n3'-UUU...GAAUU___U_______AAUUU...CAA-5'\n_____________\|___________\|\n_____________48__________36\n	266 -- 272	37 -- 43	-12.6044 kcal/mol	1.50868 kcal/mol	5.23944 kcal/mol	261 273@LB@ \| \|@LB@5'-AUU...GUUCU U GUAUG...UCG-3'@LB@ GCA GAAGUAC@LB@ \|\|\| \|\|\|\|:\|\|@LB@ CGU CUUCGUG@LB@3'-UUU...GAAUU U AAUUU...CAA-5'@LB@ \| \|@LB@ 48 36@LB@@LB@@LB@Energy -5.86 kcal/mol@LB@Hybridization Energy -12.6044 kcal/mol@LB@Unfolding Energy - mRNA 1.50868 kcal/mol@LB@Unfolding Energy - sRNA 5.23944 kcal/mol@LB@@LB@Position - mRNA 262 -- 272@LB@Position - sRNA 37 -- 47@LB@Position Seed - mRNA 266 -- 272@LB@Position Seed - sRNA 37 -- 43@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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