Input and runtime details for job 6038639 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6038639 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:35:57 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:19:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:35:30 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:35:37 CET 2018
	Job Completed		@ Fri Feb 16 21:35:51 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6038639&toolName=CopraRNA ( 30 days expiry )

Description of the job

IhtA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000117 Chlamydia trachomatis D/UW-3/CX

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
81	0.02893	0.29	ct_185	zwf	-6.95	0.147965	38 -- 47	51 -- 60	glucose-6-phosphate 1-dehydrogenase	_____________37_________48\n_____________\|__________\|\n5'-AAG...GCCUG__________AUCGU...UUU-3'\n______________UCCUCCCUGU\n______________\|\|\|:\|\|\|\|\|\|\n______________AGGGGGGACA\n3'-UUU...ACUGA__________CAUGU...GAA-5'\n_____________\|__________\|\n_____________61_________50\n	41 -- 47	51 -- 57	-17.9011 kcal/mol	1.87917 kcal/mol	9.07545 kcal/mol	37 48@LB@ \| \|@LB@5'-AAG...GCCUG AUCGU...UUU-3'@LB@ UCCUCCCUGU@LB@ \|\|\|:\|\|\|\|\|\|@LB@ AGGGGGGACA@LB@3'-UUU...ACUGA CAUGU...GAA-5'@LB@ \| \|@LB@ 61 50@LB@@LB@@LB@Energy -6.95 kcal/mol@LB@Hybridization Energy -17.9011 kcal/mol@LB@Unfolding Energy - mRNA 1.87917 kcal/mol@LB@Unfolding Energy - sRNA 9.07545 kcal/mol@LB@@LB@Position - mRNA 38 -- 47@LB@Position - sRNA 51 -- 60@LB@Position Seed - mRNA 41 -- 47@LB@Position Seed - sRNA 51 -- 57@LB@
181	0.07898	0.3587	ct_186	devB	-5.59	0.281766	201 -- 207	80 -- 86	6-phosphogluconolactonase	_____________200_____208\n_____________\|_______\|\n5'-GAA...UAUAC_______CCCUU...CAG-3'\n______________AUGGCUA\n______________\|:\|\|\|\|\|\n______________UGCCGAU\n3'-UUU...CUCUA_______UGAGG...GAA-5'\n_____________\|_______\|\n_____________87______79\n	201 -- 207	80 -- 86	-9.11344 kcal/mol	2.46127 kcal/mol	1.06449 kcal/mol	200 208@LB@ \| \|@LB@5'-GAA...UAUAC CCCUU...CAG-3'@LB@ AUGGCUA@LB@ \|:\|\|\|\|\|@LB@ UGCCGAU@LB@3'-UUU...CUCUA UGAGG...GAA-5'@LB@ \| \|@LB@ 87 79@LB@@LB@@LB@Energy -5.59 kcal/mol@LB@Hybridization Energy -9.11344 kcal/mol@LB@Unfolding Energy - mRNA 2.46127 kcal/mol@LB@Unfolding Energy - sRNA 1.06449 kcal/mol@LB@@LB@Position - mRNA 201 -- 207@LB@Position - sRNA 80 -- 86@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 80 -- 86@LB@
193	0.08525	0.3631	ct_196	N/A	-5.32	0.316248	140 -- 149	20 -- 29	inclusion membrane protein	_____________139________150\n_____________\|__________\|\n5'-UUU...AUAAU__________UUCUU...AAC-3'\n______________GCUAUUUCAU\n______________\|\|\|\|\|\|:\|\|\|\n______________CGAUAAGGUA\n3'-UUU...GUCUU__________CCGCA...GAA-5'\n_____________\|__________\|\n_____________30_________19\n	141 -- 147	22 -- 28	-11.6207 kcal/mol	1.68905 kcal/mol	4.60882 kcal/mol	139 150@LB@ \| \|@LB@5'-UUU...AUAAU UUCUU...AAC-3'@LB@ GCUAUUUCAU@LB@ \|\|\|\|\|\|:\|\|\|@LB@ CGAUAAGGUA@LB@3'-UUU...GUCUU CCGCA...GAA-5'@LB@ \| \|@LB@ 30 19@LB@@LB@@LB@Energy -5.32 kcal/mol@LB@Hybridization Energy -11.6207 kcal/mol@LB@Unfolding Energy - mRNA 1.68905 kcal/mol@LB@Unfolding Energy - sRNA 4.60882 kcal/mol@LB@@LB@Position - mRNA 140 -- 149@LB@Position - sRNA 20 -- 29@LB@Position Seed - mRNA 141 -- 147@LB@Position Seed - sRNA 22 -- 28@LB@
1	6.997e-05	0.03358	ct_198	oppA_3	-8.59	0.062291	189 -- 206	17 -- 33	oligopeptide ABC transporter substrate-binding protein	_____________188________________207\n_____________\|__________________\|\n5'-AAA...UCUAA______C________C__AAGAU...CUC-3'\n______________GGAGGU_AUUCCAUG_GC\n______________:\|\|:\|:_\|\|\|\|\|\|\|\|_\|\|\n______________UCUUCG_UAAGGUAC_CG\n3'-UUU...CUCAG______A___________CAAUC...GAA-5'\n_____________\|__________________\|\n_____________34_________________16\n	197 -- 203	19 -- 25	-19.6111 kcal/mol	6.04348 kcal/mol	4.97804 kcal/mol	188 207@LB@ \| \|@LB@5'-AAA...UCUAA C C AAGAU...CUC-3'@LB@ GGAGGU AUUCCAUG GC@LB@ :\|\|:\|: \|\|\|\|\|\|\|\| \|\|@LB@ UCUUCG UAAGGUAC CG@LB@3'-UUU...CUCAG A CAAUC...GAA-5'@LB@ \| \|@LB@ 34 16@LB@@LB@@LB@Energy -8.59 kcal/mol@LB@Hybridization Energy -19.6111 kcal/mol@LB@Unfolding Energy - mRNA 6.04348 kcal/mol@LB@Unfolding Energy - sRNA 4.97804 kcal/mol@LB@@LB@Position - mRNA 189 -- 206@LB@Position - sRNA 17 -- 33@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 19 -- 25@LB@
150	0.06394	0.3424	ct_208	gseA	-4.42	0.454874	290 -- 300	19 -- 29	3-deoxy-D-manno-octulosonic acid transferase	_____________289________300\n_____________\|__________\|\n5'-UUG...AAAGU___U_______-3'\n______________GUU_UUCCAUG\n______________\|:\|_\|\|\|\|\|\|\|\n______________CGA_AAGGUAC\n3'-UUU...GUCUU___U_______CGCAA...GAA-5'\n_____________\|___________\|\n_____________30__________18\n	294 -- 300	19 -- 25	-10.2082 kcal/mol	1.1238 kcal/mol	4.66719 kcal/mol	289 300@LB@ \| \|@LB@5'-UUG...AAAGU U -3'@LB@ GUU UUCCAUG@LB@ \|:\| \|\|\|\|\|\|\|@LB@ CGA AAGGUAC@LB@3'-UUU...GUCUU U CGCAA...GAA-5'@LB@ \| \|@LB@ 30 18@LB@@LB@@LB@Energy -4.42 kcal/mol@LB@Hybridization Energy -10.2082 kcal/mol@LB@Unfolding Energy - mRNA 1.1238 kcal/mol@LB@Unfolding Energy - sRNA 4.66719 kcal/mol@LB@@LB@Position - mRNA 290 -- 300@LB@Position - sRNA 19 -- 29@LB@Position Seed - mRNA 294 -- 300@LB@Position Seed - sRNA 19 -- 25@LB@
73	0.02714	0.29	ct_211	N/A	-9.30	0.041995	126 -- 170	20 -- 59		_____________125____________________________________________171\n_____________\|______________________________________________\|\n5'-AGA...UCAAG_____AUUCU_______AAGUC____UUUGAU_________C____ACGAA...UUU-3'\n______________CCCCU_____CUGUGUG_____CAUU_______GAAGCUAU_CCAU\n______________\|\|\|\|:_____\|\|\|\|\|\|:_____\|\|:\|_______\|\|\|\|\|\|\|\|_\|\|\|\|\n______________GGGGG_____GACACAU_____GUGA_______CUUCGAUA_GGUA\n3'-UUU...CUGAA_________________GUUU_____UCUCAGU________A____CCGCA...GAA-5'\n_____________\|______________________________________________\|\n_____________60_____________________________________________19\n	159 -- 165	25 -- 31	-32.2967 kcal/mol	9.12092 kcal/mol	13.8789 kcal/mol	125 171@LB@ \| \|@LB@5'-AGA...UCAAG AUUCU AAGUC UUUGAU C ACGAA...UUU-3'@LB@ CCCCU CUGUGUG CAUU GAAGCUAU CCAU@LB@ \|\|\|\|: \|\|\|\|\|\|: \|\|:\| \|\|\|\|\|\|\|\| \|\|\|\|@LB@ GGGGG GACACAU GUGA CUUCGAUA GGUA@LB@3'-UUU...CUGAA GUUU UCUCAGU A CCGCA...GAA-5'@LB@ \| \|@LB@ 60 19@LB@@LB@@LB@Energy -9.30 kcal/mol@LB@Hybridization Energy -32.2967 kcal/mol@LB@Unfolding Energy - mRNA 9.12092 kcal/mol@LB@Unfolding Energy - sRNA 13.8789 kcal/mol@LB@@LB@Position - mRNA 126 -- 170@LB@Position - sRNA 20 -- 59@LB@Position Seed - mRNA 159 -- 165@LB@Position Seed - sRNA 25 -- 31@LB@
157	0.06639	0.3424	ct_213	rpiA	-7.48	0.112460	245 -- 274	28 -- 57	ribose-5-phosphate isomerase A	_____________244____________________________275\n_____________\|______________________________\|\n5'-GAA...UUUUU____AUCAA_____A_____C_C_______AGUAG...UGU-3'\n______________CCCU_____GCAGA_ACUAG_G_UAGAGGC\n______________\|\|\|:_____:\|\|:\|_\|\|\|\|\|_\|_:\|\|\|:\|\|\n______________GGGG_____UGUUU_UGAUC_C_GUCUUCG\n3'-UUU...GAAGG____ACACA_____G_____U_A_______AUAAG...GAA-5'\n_____________\|______________________________\|\n_____________58_____________________________27\n	268 -- 274	28 -- 34	-25.4315 kcal/mol	5.87242 kcal/mol	12.0748 kcal/mol	244 275@LB@ \| \|@LB@5'-GAA...UUUUU AUCAA A C C AGUAG...UGU-3'@LB@ CCCU GCAGA ACUAG G UAGAGGC@LB@ \|\|\|: :\|\|:\| \|\|\|\|\| \| :\|\|\|:\|\|@LB@ GGGG UGUUU UGAUC C GUCUUCG@LB@3'-UUU...GAAGG ACACA G U A AUAAG...GAA-5'@LB@ \| \|@LB@ 58 27@LB@@LB@@LB@Energy -7.48 kcal/mol@LB@Hybridization Energy -25.4315 kcal/mol@LB@Unfolding Energy - mRNA 5.87242 kcal/mol@LB@Unfolding Energy - sRNA 12.0748 kcal/mol@LB@@LB@Position - mRNA 245 -- 274@LB@Position - sRNA 28 -- 57@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 28 -- 34@LB@
86	0.03096	0.29	ct_221	yqfU	-7.15	0.133289	38 -- 44	80 -- 86		_____________37______45\n_____________\|_______\|\n5'-AUA...GCUCA_______GAGCA...GUG-3'\n______________GCGGUUA\n______________:\|\|\|:\|\|\n______________UGCCGAU\n3'-UUU...CUCUA_______UGAGG...GAA-5'\n_____________\|_______\|\n_____________87______79\n	38 -- 44	80 -- 86	-8.46136 kcal/mol	0.243281 kcal/mol	1.06449 kcal/mol	37 45@LB@ \| \|@LB@5'-AUA...GCUCA GAGCA...GUG-3'@LB@ GCGGUUA@LB@ :\|\|\|:\|\|@LB@ UGCCGAU@LB@3'-UUU...CUCUA UGAGG...GAA-5'@LB@ \| \|@LB@ 87 79@LB@@LB@@LB@Energy -7.15 kcal/mol@LB@Hybridization Energy -8.46136 kcal/mol@LB@Unfolding Energy - mRNA 0.243281 kcal/mol@LB@Unfolding Energy - sRNA 1.06449 kcal/mol@LB@@LB@Position - mRNA 38 -- 44@LB@Position - sRNA 80 -- 86@LB@Position Seed - mRNA 38 -- 44@LB@Position Seed - sRNA 80 -- 86@LB@
117	0.04834	0.3262	ct_237	fabG	-8.76	0.056710	143 -- 164	34 -- 58	3-oxoacyl-[acyl-carrier-protein] reductase	_____________142________________________165\n_____________\|__________________________\|\n5'-CGU...GAGUU_________________U________UAUGG...AUG-3'\n______________CCCCUUGU____AAACA_UUAGGGUC\n______________\|\|\|\|:\|\|\|____\|\|\|\|\|_:\|\|\|:\|\|\|\n______________GGGGGACA____UUUGU_GAUCUCAG\n3'-UUU...UGAAG________CAUG______________UCUUC...GAA-5'\n_____________\|__________________________\|\n_____________59_________________________33\n	144 -- 150	51 -- 57	-24.3621 kcal/mol	7.62467 kcal/mol	7.97757 kcal/mol	142 165@LB@ \| \|@LB@5'-CGU...GAGUU U UAUGG...AUG-3'@LB@ CCCCUUGU AAACA UUAGGGUC@LB@ \|\|\|\|:\|\|\| \|\|\|\|\| :\|\|\|:\|\|\|@LB@ GGGGGACA UUUGU GAUCUCAG@LB@3'-UUU...UGAAG CAUG UCUUC...GAA-5'@LB@ \| \|@LB@ 59 33@LB@@LB@@LB@Energy -8.76 kcal/mol@LB@Hybridization Energy -24.3621 kcal/mol@LB@Unfolding Energy - mRNA 7.62467 kcal/mol@LB@Unfolding Energy - sRNA 7.97757 kcal/mol@LB@@LB@Position - mRNA 143 -- 164@LB@Position - sRNA 34 -- 58@LB@Position Seed - mRNA 144 -- 150@LB@Position Seed - sRNA 51 -- 57@LB@
147	0.05913	0.3307	ct_243	lpxD	-5.18	0.335797	179 -- 192	18 -- 32	UDP-3-O-acylglucosamine N-acyltransferase	_____________178_____________193\n_____________\|_______________\|\n5'-UCA...CAUGC_______G____A__AGUGC...UCG-3'\n______________GAAGCUA__CCG_GG\n______________\|\|\|\|\|\|\|__\|\|:_\|\|\n______________CUUCGAU__GGU_CC\n3'-UUU...UCAGU_______AA___A__GCAAU...GAA-5'\n_____________\|_______________\|\n_____________33______________17\n	179 -- 185	26 -- 32	-15.005 kcal/mol	4.85774 kcal/mol	4.96566 kcal/mol	178 193@LB@ \| \|@LB@5'-UCA...CAUGC G A AGUGC...UCG-3'@LB@ GAAGCUA CCG GG@LB@ \|\|\|\|\|\|\| \|\|: \|\|@LB@ CUUCGAU GGU CC@LB@3'-UUU...UCAGU AA A GCAAU...GAA-5'@LB@ \| \|@LB@ 33 17@LB@@LB@@LB@Energy -5.18 kcal/mol@LB@Hybridization Energy -15.005 kcal/mol@LB@Unfolding Energy - mRNA 4.85774 kcal/mol@LB@Unfolding Energy - sRNA 4.96566 kcal/mol@LB@@LB@Position - mRNA 179 -- 192@LB@Position - sRNA 18 -- 32@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 26 -- 32@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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