Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
168	0.008282	0.1723	b3822	recQ	-12.50	0.081546	124 -- 155	43 -- 77	ATP-dependent DNA helicase	_____________123___________________________________156\n_____________\|_____________________________________\|\n5'-CGC...UCUCA_______AAA____U_______C_________G____CGCUG...UUA-3'\n______________GAGACUA___GCAU_GCAGUUU______UCGC_CAGG\n______________:\|\|\|\|\|\|___:\|\|:_\|\|\|:\|\|:______\|\|:\|_\|\|\|\|\n______________UUCUGAU___UGUG_CGUUAAG______AGUG_GUCC\n3'-UUU...CCCGU_______G______U_______CAACAA____A____CCUAA...GCA-5'\n_____________\|_____________________________________\|\n_____________78____________________________________42\n	139 -- 145	58 -- 64	-25.7534 kcal/mol	8.90299 kcal/mol	4.3531 kcal/mol	123 156@LB@ \| \|@LB@5'-CGC...UCUCA AAA U C G CGCUG...UUA-3'@LB@ GAGACUA GCAU GCAGUUU UCGC CAGG@LB@ :\|\|\|\|\|\| :\|\|: \|\|\|:\|\|: \|\|:\| \|\|\|\|@LB@ UUCUGAU UGUG CGUUAAG AGUG GUCC@LB@3'-UUU...CCCGU G U CAACAA A CCUAA...GCA-5'@LB@ \| \|@LB@ 78 42@LB@@LB@@LB@Energy -12.50 kcal/mol@LB@Hybridization Energy -25.7534 kcal/mol@LB@Unfolding Energy - mRNA 8.90299 kcal/mol@LB@Unfolding Energy - sRNA 4.3531 kcal/mol@LB@@LB@Position - mRNA 124 -- 155@LB@Position - sRNA 43 -- 77@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 58 -- 64@LB@
99	0.004523	0.1538	b1516	lsrB	-10.33	0.215118	8 -- 28	39 -- 59	autoinducer 2-binding protein	_____________7______________________29\n_____________\|______________________\|\n____5'-ACCGCCA___CG___________U_____AUUUG...UUC-3'\n______________UUG__GUUUUAUU_AG_GGGAU\n______________\|:\|__\|\|\|\|:\|:\|_\|\|_\|\|\|\|\|\n______________AGC__CAAAGUGA_UC_CCCUA\n3'-UUU...CGUUA___AA________G________AAGGU...GCA-5'\n_____________\|______________________\|\n_____________60_____________________38\n	14 -- 20	47 -- 53	-16.4048 kcal/mol	3.58309 kcal/mol	2.49358 kcal/mol	7 29@LB@ \| \|@LB@ 5'-ACCGCCA CG U AUUUG...UUC-3'@LB@ UUG GUUUUAUU AG GGGAU@LB@ \|:\| \|\|\|\|:\|:\| \|\| \|\|\|\|\|@LB@ AGC CAAAGUGA UC CCCUA@LB@3'-UUU...CGUUA AA G AAGGU...GCA-5'@LB@ \| \|@LB@ 60 38@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -16.4048 kcal/mol@LB@Unfolding Energy - mRNA 3.58309 kcal/mol@LB@Unfolding Energy - sRNA 2.49358 kcal/mol@LB@@LB@Position - mRNA 8 -- 28@LB@Position - sRNA 39 -- 59@LB@Position Seed - mRNA 14 -- 20@LB@Position Seed - sRNA 47 -- 53@LB@
11	9.086e-05	0.0245	b3337	bfd	-12.11	0.098410	247 -- 280	34 -- 76	bacterioferritin-associated ferredoxin	_____________246_________________________________________281\n_____________\|___________________________________________\|\n5'-GCG...UCGUC___________________UCCCC___________________GCAAU...UUC-3'\n______________AGGCUGUGCGC__CAGUUU______UCACUCG______UUCCA\n______________\|\|:\|\|:::\|:\|__\|\|:\|\|:______\|\|\|\|\|\|:______\|\|\|\|\|\n______________UCUGAUGUGUG__GUUAAG______AGUGAGU______AAGGU\n3'-UUU...CCGUU___________UC______CAACAA_______CCCCUA_____ACGAA...GCA-5'\n_____________\|___________________________________________\|\n_____________77__________________________________________33\n	269 -- 275	45 -- 51	-24.9474 kcal/mol	8.02269 kcal/mol	4.816 kcal/mol	246 281@LB@ \| \|@LB@5'-GCG...UCGUC UCCCC GCAAU...UUC-3'@LB@ AGGCUGUGCGC CAGUUU UCACUCG UUCCA@LB@ \|\|:\|\|:::\|:\| \|\|:\|\|: \|\|\|\|\|\|: \|\|\|\|\|@LB@ UCUGAUGUGUG GUUAAG AGUGAGU AAGGU@LB@3'-UUU...CCGUU UC CAACAA CCCCUA ACGAA...GCA-5'@LB@ \| \|@LB@ 77 33@LB@@LB@@LB@Energy -12.11 kcal/mol@LB@Hybridization Energy -24.9474 kcal/mol@LB@Unfolding Energy - mRNA 8.02269 kcal/mol@LB@Unfolding Energy - sRNA 4.816 kcal/mol@LB@@LB@Position - mRNA 247 -- 280@LB@Position - sRNA 34 -- 76@LB@Position Seed - mRNA 269 -- 275@LB@Position Seed - sRNA 45 -- 51@LB@
103	0.004595	0.1538	b3554	yiaF	-10.61	0.191588	8 -- 38	46 -- 75	barrier effect co-colonization resistance factor; DUF3053 family lipoprotein	_____________7_________________________________39\n_____________\|_________________________________\|\n____5'-AAUCUCU_______AAAAA_________GCACUA______UUUAA...AGC-3'\n______________GGCUACA_____GGCAA__CG______UCAUUU\n______________\|:\|\|\|\|\|_____:\|\|\|\|__\|\|______\|\|\|:\|:\n______________CUGAUGU_____UCGUU__GC______AGUGAG\n3'-UUU...CGUUU_______GUG_______AA__AACAA_______UCCCC...GCA-5'\n_____________\|_________________________________\|\n_____________76________________________________45\n	8 -- 14	69 -- 75	-17.9003 kcal/mol	3.5618 kcal/mol	3.72616 kcal/mol	7 39@LB@ \| \|@LB@ 5'-AAUCUCU AAAAA GCACUA UUUAA...AGC-3'@LB@ GGCUACA GGCAA CG UCAUUU@LB@ \|:\|\|\|\|\| :\|\|\|\| \|\| \|\|\|:\|:@LB@ CUGAUGU UCGUU GC AGUGAG@LB@3'-UUU...CGUUU GUG AA AACAA UCCCC...GCA-5'@LB@ \| \|@LB@ 76 45@LB@@LB@@LB@Energy -10.61 kcal/mol@LB@Hybridization Energy -17.9003 kcal/mol@LB@Unfolding Energy - mRNA 3.5618 kcal/mol@LB@Unfolding Energy - sRNA 3.72616 kcal/mol@LB@@LB@Position - mRNA 8 -- 38@LB@Position - sRNA 46 -- 75@LB@Position Seed - mRNA 8 -- 14@LB@Position Seed - sRNA 69 -- 75@LB@
196	0.01003	0.1776	b0344	lacZ	-8.86	0.369200	215 -- 237	48 -- 76	beta-D-galactosidase	_____________214___________________________238\n_____________\|_____________________________\|\n5'-ACC...AUUAC_____________________C_______AACGU...CUU-3'\n______________GGAUU_CAC_UGGC____CGU_GUUUUAC\n______________:\|\|:\|_\|\|\|_::\|\|____\|\|\|_\|\|\|\|:\|\|\n______________UCUGA_GUG_GUCG____GCA_CAAAGUG\n3'-UUU...CCGUU_____U___U____UUAA___A_______AGUCC...GCA-5'\n_____________\|_____________________________\|\n_____________77____________________________47\n	231 -- 237	48 -- 54	-17.9156 kcal/mol	5.37156 kcal/mol	3.68714 kcal/mol	214 238@LB@ \| \|@LB@5'-ACC...AUUAC C AACGU...CUU-3'@LB@ GGAUU CAC UGGC CGU GUUUUAC@LB@ :\|\|:\| \|\|\| ::\|\| \|\|\| \|\|\|\|:\|\|@LB@ UCUGA GUG GUCG GCA CAAAGUG@LB@3'-UUU...CCGUU U U UUAA A AGUCC...GCA-5'@LB@ \| \|@LB@ 77 47@LB@@LB@@LB@Energy -8.86 kcal/mol@LB@Hybridization Energy -17.9156 kcal/mol@LB@Unfolding Energy - mRNA 5.37156 kcal/mol@LB@Unfolding Energy - sRNA 3.68714 kcal/mol@LB@@LB@Position - mRNA 215 -- 237@LB@Position - sRNA 48 -- 76@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 48 -- 54@LB@
53	0.001859	0.1218	b0529	folD	-11.41	0.136116	200 -- 226	61 -- 85	bifunctional 5 10-methylene-tetrahydrofolate dehydrogenase/ 5 10-methylene-tetrahydrofolate cyclohydrolase	_____________199_________________________227\n_____________\|___________________________\|\n5'-CAA...UCUCU_____CA___________UG_______AACGA...CAC-3'\n______________GAUGG__GCAAAGAUUAU__ACGGUAA\n______________\|\|\|\|\|__\|\|\|\|\|\|\|:\|\|:__\|\|:\|:\|\|\n______________CUACC__CGUUUCUGAUG__UGUCGUU\n3'-UUU...ACCCU__________________UG_______AAGCA...GCA-5'\n_____________\|___________________________\|\n_____________86__________________________60\n	220 -- 226	61 -- 67	-27.4215 kcal/mol	5.77154 kcal/mol	10.2439 kcal/mol	199 227@LB@ \| \|@LB@5'-CAA...UCUCU CA UG AACGA...CAC-3'@LB@ GAUGG GCAAAGAUUAU ACGGUAA@LB@ \|\|\|\|\| \|\|\|\|\|\|\|:\|\|: \|\|:\|:\|\|@LB@ CUACC CGUUUCUGAUG UGUCGUU@LB@3'-UUU...ACCCU UG AAGCA...GCA-5'@LB@ \| \|@LB@ 86 60@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -27.4215 kcal/mol@LB@Unfolding Energy - mRNA 5.77154 kcal/mol@LB@Unfolding Energy - sRNA 10.2439 kcal/mol@LB@@LB@Position - mRNA 200 -- 226@LB@Position - sRNA 61 -- 85@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 61 -- 67@LB@
114	0.005004	0.1545	b0025	ribF	-11.63	0.123109	210 -- 237	43 -- 71	bifunctional riboflavin kinase/FAD synthetase	_____________209___________________________238\n_____________\|_____________________________\|\n5'-UGG...AGCUG__________UACAUAAUC____GC____CCCCG...GCG-3'\n______________AUACGCGGCA__________UCA__CAGG\n______________\|:\|\|:\|:\|\|\|__________\|\|\|__\|\|\|\|\n______________UGUGUGUCGU__________AGU__GUCC\n3'-UUU...UCUGA__________UAAGCAACAA___GA____CCUAA...GCA-5'\n_____________\|_____________________________\|\n_____________72____________________________42\n	213 -- 219	62 -- 68	-21.1915 kcal/mol	5.23887 kcal/mol	4.32409 kcal/mol	209 238@LB@ \| \|@LB@5'-UGG...AGCUG UACAUAAUC GC CCCCG...GCG-3'@LB@ AUACGCGGCA UCA CAGG@LB@ \|:\|\|:\|:\|\|\| \|\|\| \|\|\|\|@LB@ UGUGUGUCGU AGU GUCC@LB@3'-UUU...UCUGA UAAGCAACAA GA CCUAA...GCA-5'@LB@ \| \|@LB@ 72 42@LB@@LB@@LB@Energy -11.63 kcal/mol@LB@Hybridization Energy -21.1915 kcal/mol@LB@Unfolding Energy - mRNA 5.23887 kcal/mol@LB@Unfolding Energy - sRNA 4.32409 kcal/mol@LB@@LB@Position - mRNA 210 -- 237@LB@Position - sRNA 43 -- 71@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 62 -- 68@LB@
67	0.002719	0.1417	b3454	livF	-11.67	0.120634	106 -- 122	40 -- 58	branched-chain amino acid ABC transporter ATPase	_____________105_________________123\n_____________\|___________________\|\n5'-AGA...AUUUA___GG_____A________CGCCG...UUG-3'\n______________CGU____UCA_UCAGGGGA\n______________\|\|\|____\|\|\|_\|\|\|\|\|\|\|\|\n______________GCA____AGU_AGUCCCCU\n3'-UUU...GUUAA___ACAA___G________AAAGG...GCA-5'\n_____________\|___________________\|\n_____________59__________________39\n	116 -- 122	40 -- 46	-19.8465 kcal/mol	5.75116 kcal/mol	2.42241 kcal/mol	105 123@LB@ \| \|@LB@5'-AGA...AUUUA GG A CGCCG...UUG-3'@LB@ CGU UCA UCAGGGGA@LB@ \|\|\| \|\|\| \|\|\|\|\|\|\|\|@LB@ GCA AGU AGUCCCCU@LB@3'-UUU...GUUAA ACAA G AAAGG...GCA-5'@LB@ \| \|@LB@ 59 39@LB@@LB@@LB@Energy -11.67 kcal/mol@LB@Hybridization Energy -19.8465 kcal/mol@LB@Unfolding Energy - mRNA 5.75116 kcal/mol@LB@Unfolding Energy - sRNA 2.42241 kcal/mol@LB@@LB@Position - mRNA 106 -- 122@LB@Position - sRNA 40 -- 58@LB@Position Seed - mRNA 116 -- 122@LB@Position Seed - sRNA 40 -- 46@LB@
140	0.006445	0.1605	b0325	yahK	-13.93	0.038719	67 -- 79	43 -- 55	broad specificity NADPH-dependent aldehyde reductase Zn-containing	_____________66____________80\n_____________\|_____________\|\n5'-UUA...UGCUC_____________CUCGC...AAA-3'\n______________UGUUUCAUUCAGG\n______________\|\|\|\|\|\|\|:\|\|\|\|\|\n______________ACAAAGUGAGUCC\n3'-UUU...AAGCA_____________CCUAA...GCA-5'\n_____________\|_____________\|\n_____________56____________42\n	73 -- 79	43 -- 49	-18.9036 kcal/mol	3.47822 kcal/mol	1.49792 kcal/mol	66 80@LB@ \| \|@LB@5'-UUA...UGCUC CUCGC...AAA-3'@LB@ UGUUUCAUUCAGG@LB@ \|\|\|\|\|\|\|:\|\|\|\|\|@LB@ ACAAAGUGAGUCC@LB@3'-UUU...AAGCA CCUAA...GCA-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -13.93 kcal/mol@LB@Hybridization Energy -18.9036 kcal/mol@LB@Unfolding Energy - mRNA 3.47822 kcal/mol@LB@Unfolding Energy - sRNA 1.49792 kcal/mol@LB@@LB@Position - mRNA 67 -- 79@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 43 -- 49@LB@
124	0.005471	0.1553	b1249	clsA	-8.47	0.418250	164 -- 196	34 -- 71	cardiolipin synthase 1	_____________163____________________________________197\n_____________\|______________________________________\|\n5'-GUC...CGUAA__U____A____G__C_______AAA____________AUCUA...CAG-3'\n______________AC_CAUA_CAAU_CG___UUUCA______GGAUUUCUA\n______________\|\|_\|\|:\|_\|\|\|\|_\|\|___\|\|\|\|\|______\|\|\|\|\|\|\|:\|\n______________UG_GUGU_GUUA_GC___AAAGU______CCUAAAGGU\n3'-UUU...UCUGA__U____C____A__AAC_____GAGUCC_________ACGAA...GCA-5'\n_____________\|______________________________________\|\n_____________72_____________________________________33\n	190 -- 196	34 -- 40	-20.3655 kcal/mol	7.10457 kcal/mol	4.79237 kcal/mol	163 197@LB@ \| \|@LB@5'-GUC...CGUAA U A G C AAA AUCUA...CAG-3'@LB@ AC CAUA CAAU CG UUUCA GGAUUUCUA@LB@ \|\| \|\|:\| \|\|\|\| \|\| \|\|\|\|\| \|\|\|\|\|\|\|:\|@LB@ UG GUGU GUUA GC AAAGU CCUAAAGGU@LB@3'-UUU...UCUGA U C A AAC GAGUCC ACGAA...GCA-5'@LB@ \| \|@LB@ 72 33@LB@@LB@@LB@Energy -8.47 kcal/mol@LB@Hybridization Energy -20.3655 kcal/mol@LB@Unfolding Energy - mRNA 7.10457 kcal/mol@LB@Unfolding Energy - sRNA 4.79237 kcal/mol@LB@@LB@Position - mRNA 164 -- 196@LB@Position - sRNA 34 -- 71@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 34 -- 40@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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