Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
22	0.0001899	0.02926	b2562	yfhL	-14.77	0.023959	28 -- 42	42 -- 56	putative 4Fe-4S cluster-containing protein		_____________27______________43\n_____________\|_______________\|\n5'-AGC...UCUGC____CA_________AAUGU...UUA-3'\n______________UUGU__CGCUCAGGG\n______________\|\|\|\|__\|:\|\|\|\|\|\|\|\n______________AACA__GUGAGUCCC\n3'-UUU...UAAGC____AA_________CUAAA...GCA-5'\n_____________\|_______________\|\n_____________57______________41\n	36 -- 42	42 -- 48	-19.6539 kcal/mol	2.98712 kcal/mol	1.89561 kcal/mol	27 43@LB@ \| \|@LB@5'-AGC...UCUGC CA AAUGU...UUA-3'@LB@ UUGU CGCUCAGGG@LB@ \|\|\|\| \|:\|\|\|\|\|\|\|@LB@ AACA GUGAGUCCC@LB@3'-UUU...UAAGC AA CUAAA...GCA-5'@LB@ \| \|@LB@ 57 41@LB@@LB@@LB@Energy -14.77 kcal/mol@LB@Hybridization Energy -19.6539 kcal/mol@LB@Unfolding Energy - mRNA 2.98712 kcal/mol@LB@Unfolding Energy - sRNA 1.89561 kcal/mol@LB@@LB@Position - mRNA 28 -- 42@LB@Position - sRNA 42 -- 56@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 42 -- 48@LB@
160	0.007693	0.1668	b1671	ydhX	-11.21	0.148807	210 -- 235	39 -- 74	putative 4Fe-4S ferridoxin-type protein; FNR Nar NarP-regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex	b4072	_____________209__________________________________236\n_____________\|____________________________________\|\n5'-CAC...CCUUC___CG________________________U______GGGAA...AGG-3'\n______________ACU__ACGCA__AAUUUGU________UC_GGGGAU\n______________\|\|\|__\|\|:\|\|__\|\|\|\|:\|\|________\|\|_\|\|\|\|\|\|\n______________UGA__UGUGU__UUAAGCA________AG_CCCCUA\n3'-UUU...GUUUC___UG_____CG_______ACAAAGUG__U______AAGGU...GCA-5'\n_____________\|____________________________________\|\n_____________75___________________________________38\n	220 -- 226	56 -- 62	-21.5478 kcal/mol	5.56457 kcal/mol	4.77831 kcal/mol	209 236@LB@ \| \|@LB@5'-CAC...CCUUC CG U GGGAA...AGG-3'@LB@ ACU ACGCA AAUUUGU UC GGGGAU@LB@ \|\|\| \|\|:\|\| \|\|\|\|:\|\| \|\| \|\|\|\|\|\|@LB@ UGA UGUGU UUAAGCA AG CCCCUA@LB@3'-UUU...GUUUC UG CG ACAAAGUG U AAGGU...GCA-5'@LB@ \| \|@LB@ 75 38@LB@@LB@@LB@Energy -11.21 kcal/mol@LB@Hybridization Energy -21.5478 kcal/mol@LB@Unfolding Energy - mRNA 5.56457 kcal/mol@LB@Unfolding Energy - sRNA 4.77831 kcal/mol@LB@@LB@Position - mRNA 210 -- 235@LB@Position - sRNA 39 -- 74@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 56 -- 62@LB@
130	0.005881	0.1577	b0965	yccU	-10.68	0.186123	93 -- 125	46 -- 74	putative CoA-binding protein		_____________92________________________________126\n_____________\|_________________________________\|\n5'-UCA...UACUU___A________A__UAU___CCGC________UCAUU...UGA-3'\n______________ACU_ACAUAUAG_CA___UUC____UUUUGCUC\n______________\|\|\|_\|\|\|:\|:\|\|_\|\|___\|\|\|____\|\|\|::\|\|\|\n______________UGA_UGUGUGUC_GU___AAG____AAAGUGAG\n3'-UUU...GUUUC_______________U_____CAAC________UCCCC...GCA-5'\n_____________\|_________________________________\|\n_____________75________________________________45\n	119 -- 125	46 -- 52	-18.0647 kcal/mol	3.65669 kcal/mol	3.72594 kcal/mol	92 126@LB@ \| \|@LB@5'-UCA...UACUU A A UAU CCGC UCAUU...UGA-3'@LB@ ACU ACAUAUAG CA UUC UUUUGCUC@LB@ \|\|\| \|\|\|:\|:\|\| \|\| \|\|\| \|\|\|::\|\|\|@LB@ UGA UGUGUGUC GU AAG AAAGUGAG@LB@3'-UUU...GUUUC U CAAC UCCCC...GCA-5'@LB@ \| \|@LB@ 75 45@LB@@LB@@LB@Energy -10.68 kcal/mol@LB@Hybridization Energy -18.0647 kcal/mol@LB@Unfolding Energy - mRNA 3.65669 kcal/mol@LB@Unfolding Energy - sRNA 3.72594 kcal/mol@LB@@LB@Position - mRNA 93 -- 125@LB@Position - sRNA 46 -- 74@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 46 -- 52@LB@
35	0.0005607	0.05638	b1434	ydcN	-11.45	0.133710	242 -- 253	62 -- 74	putative DNA-binding transcriptional regulator	b1508 b1299	_____________241___________254\n_____________\|_____________\|\n5'-CGG...AAACA_____________GCGCG...CUA-3'\n______________ACUACGC_CAGCA\n______________\|\|\|\|\|:\|_\|\|\|\|\|\n______________UGAUGUG_GUCGU\n3'-UUU...GUUUC_______U_____UAAGC...GCA-5'\n_____________\|_____________\|\n_____________75____________61\n	242 -- 248	68 -- 74	-17.054 kcal/mol	2.36402 kcal/mol	3.24359 kcal/mol	241 254@LB@ \| \|@LB@5'-CGG...AAACA GCGCG...CUA-3'@LB@ ACUACGC CAGCA@LB@ \|\|\|\|\|:\| \|\|\|\|\|@LB@ UGAUGUG GUCGU@LB@3'-UUU...GUUUC U UAAGC...GCA-5'@LB@ \| \|@LB@ 75 61@LB@@LB@@LB@Energy -11.45 kcal/mol@LB@Hybridization Energy -17.054 kcal/mol@LB@Unfolding Energy - mRNA 2.36402 kcal/mol@LB@Unfolding Energy - sRNA 3.24359 kcal/mol@LB@@LB@Position - mRNA 242 -- 253@LB@Position - sRNA 62 -- 74@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 68 -- 74@LB@
100	0.004525	0.1538	b2550	yphH	-8.03	0.477033	31 -- 47	39 -- 54	putative DNA-binding transcriptional regulator		_____________30_________________48\n_____________\|__________________\|\n5'-AGG...GCCCG_______A___UC_____CUCAA...UGG-3'\n______________GUUUCAU__GG__GGGAU\n______________\|\|\|\|\|\|:__:\|__\|\|\|\|\|\n______________CAAAGUG__UC__CCCUA\n3'-UUU...AGCAA_______AG_________AAGGU...GCA-5'\n_____________\|__________________\|\n_____________55_________________38\n	31 -- 37	48 -- 54	-12.873 kcal/mol	2.94673 kcal/mol	1.89674 kcal/mol	30 48@LB@ \| \|@LB@5'-AGG...GCCCG A UC CUCAA...UGG-3'@LB@ GUUUCAU GG GGGAU@LB@ \|\|\|\|\|\|: :\| \|\|\|\|\|@LB@ CAAAGUG UC CCCUA@LB@3'-UUU...AGCAA AG AAGGU...GCA-5'@LB@ \| \|@LB@ 55 38@LB@@LB@@LB@Energy -8.03 kcal/mol@LB@Hybridization Energy -12.873 kcal/mol@LB@Unfolding Energy - mRNA 2.94673 kcal/mol@LB@Unfolding Energy - sRNA 1.89674 kcal/mol@LB@@LB@Position - mRNA 31 -- 47@LB@Position - sRNA 39 -- 54@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 48 -- 54@LB@
174	0.008627	0.1745	b1926	fliT	-7.57	0.540698	180 -- 211	31 -- 53	putative flagellar synthesis and assembly chaperone		_____________179______________________________212\n_____________\|________________________________\|\n5'-GUG...CCUUC___G_______ACCCAGUCU___GAA______ACCGC...UCA-3'\n______________UUU_AUUCAGG_________GAU___CCAUGC\n______________\|\|\|_\|:\|\|\|\|\|_________\|\|\|___\|\|\|\|\|\|\n______________AAA_UGAGUCC_________CUA___GGUACG\n3'-UUU...GCAAC___G_______C___________AA_______AAUAU...GCA-5'\n_____________\|________________________________\|\n_____________54_______________________________30\n	184 -- 190	43 -- 49	-18.5651 kcal/mol	6.60729 kcal/mol	4.38339 kcal/mol	179 212@LB@ \| \|@LB@5'-GUG...CCUUC G ACCCAGUCU GAA ACCGC...UCA-3'@LB@ UUU AUUCAGG GAU CCAUGC@LB@ \|\|\| \|:\|\|\|\|\| \|\|\| \|\|\|\|\|\|@LB@ AAA UGAGUCC CUA GGUACG@LB@3'-UUU...GCAAC G C AA AAUAU...GCA-5'@LB@ \| \|@LB@ 54 30@LB@@LB@@LB@Energy -7.57 kcal/mol@LB@Hybridization Energy -18.5651 kcal/mol@LB@Unfolding Energy - mRNA 6.60729 kcal/mol@LB@Unfolding Energy - sRNA 4.38339 kcal/mol@LB@@LB@Position - mRNA 180 -- 211@LB@Position - sRNA 31 -- 53@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 43 -- 49@LB@
175	0.008768	0.1747	b0658	ybeX	-12.37	0.086971	210 -- 256	35 -- 75	putative ion transport		_____________209_____________________________________________257\n_____________\|_______________________________________________\|\n5'-UUA...GCGAC___AAUU___________GACACGAUAAG__ACA__AA_________CUCCC...ACC-3'\n______________GAC____CACACAGUAGU___________CA___AG__GGGAUUUUU\n______________\|\|\|____\|\|\|\|\|\|\|:\|:\|___________\|\|___\|\|__\|\|\|\|\|\|\|::\n______________CUG____GUGUGUCGUUA___________GU___UC__CCCUAAAGG\n3'-UUU...CGUUU___AU_____________AGCAACAAA____GAG_____________UACGA...GCA-5'\n_____________\|_______________________________________________\|\n_____________76______________________________________________34\n	249 -- 255	36 -- 42	-26.9454 kcal/mol	9.77444 kcal/mol	4.80541 kcal/mol	209 257@LB@ \| \|@LB@5'-UUA...GCGAC AAUU GACACGAUAAG ACA AA CUCCC...ACC-3'@LB@ GAC CACACAGUAGU CA AG GGGAUUUUU@LB@ \|\|\| \|\|\|\|\|\|\|:\|:\| \|\| \|\| \|\|\|\|\|\|\|::@LB@ CUG GUGUGUCGUUA GU UC CCCUAAAGG@LB@3'-UUU...CGUUU AU AGCAACAAA GAG UACGA...GCA-5'@LB@ \| \|@LB@ 76 34@LB@@LB@@LB@Energy -12.37 kcal/mol@LB@Hybridization Energy -26.9454 kcal/mol@LB@Unfolding Energy - mRNA 9.77444 kcal/mol@LB@Unfolding Energy - sRNA 4.80541 kcal/mol@LB@@LB@Position - mRNA 210 -- 256@LB@Position - sRNA 35 -- 75@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 36 -- 42@LB@
76	0.003141	0.1452	b0187	yaeR	-8.98	0.354982	261 -- 292	48 -- 78	putative lyase		_____________260_______________________________293\n_____________\|_________________________________\|\n5'-AAA...UGAGC_______UUC___U_____AU__UUG_______GCUGCAAA-3'\n______________AAAG_CU___UAC_GCGAU__UC___GUUUCAC\n______________\|\|\|\|_\|\|___:\|\|_\|\|:\|\|__\|\|___\|\|\|\|\|\|\|\n______________UUUC_GA___GUG_CGUUA__AG___CAAAGUG\n3'-UUU...ACCCG____U__UGU___U_________CAA_______AGUCC...GCA-5'\n_____________\|_________________________________\|\n_____________79________________________________47\n	286 -- 292	48 -- 54	-17.4111 kcal/mol	4.74942 kcal/mol	3.68736 kcal/mol	260 293@LB@ \| \|@LB@5'-AAA...UGAGC UUC U AU UUG GCUGCAAA-3'@LB@ AAAG CU UAC GCGAU UC GUUUCAC@LB@ \|\|\|\| \|\| :\|\| \|\|:\|\| \|\| \|\|\|\|\|\|\|@LB@ UUUC GA GUG CGUUA AG CAAAGUG@LB@3'-UUU...ACCCG U UGU U CAA AGUCC...GCA-5'@LB@ \| \|@LB@ 79 47@LB@@LB@@LB@Energy -8.98 kcal/mol@LB@Hybridization Energy -17.4111 kcal/mol@LB@Unfolding Energy - mRNA 4.74942 kcal/mol@LB@Unfolding Energy - sRNA 3.68736 kcal/mol@LB@@LB@Position - mRNA 261 -- 292@LB@Position - sRNA 48 -- 78@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 48 -- 54@LB@
189	0.009605	0.1776	b0927	ycbL	-15.21	0.018444	14 -- 51	33 -- 78	putative metal-binding enzyme		_____________13__________________________________________________52\n_____________\|___________________________________________________\|\n5'-GGA...GAAGA________U_____UAAAG__________________CGAU__________AUUGA...GCG-3'\n______________AAAG_CUA_CACAC________________GC_CAGG____GGAUUUCCAU\n______________\|\|\|\|_\|\|\|_\|\|\|\|\|________________:\|_\|\|\|\|____\|\|\|\|\|\|\|\|\|\|\n______________UUUC_GAU_GUGUG________________UG_GUCC____CCUAAAGGUA\n3'-UUU...ACCCG____U_________UCGUUAAGCAACAAAG__A__________________CGAAU...GCA-5'\n_____________\|___________________________________________________\|\n_____________79__________________________________________________32\n	45 -- 51	33 -- 39	-26.8189 kcal/mol	6.79395 kcal/mol	4.81602 kcal/mol	13 52@LB@ \| \|@LB@5'-GGA...GAAGA U UAAAG CGAU AUUGA...GCG-3'@LB@ AAAG CUA CACAC GC CAGG GGAUUUCCAU@LB@ \|\|\|\| \|\|\| \|\|\|\|\| :\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|@LB@ UUUC GAU GUGUG UG GUCC CCUAAAGGUA@LB@3'-UUU...ACCCG U UCGUUAAGCAACAAAG A CGAAU...GCA-5'@LB@ \| \|@LB@ 79 32@LB@@LB@@LB@Energy -15.21 kcal/mol@LB@Hybridization Energy -26.8189 kcal/mol@LB@Unfolding Energy - mRNA 6.79395 kcal/mol@LB@Unfolding Energy - sRNA 4.81602 kcal/mol@LB@@LB@Position - mRNA 14 -- 51@LB@Position - sRNA 33 -- 78@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 33 -- 39@LB@
136	0.006258	0.1605	b0219	yafV	-11.82	0.112632	212 -- 233	61 -- 78	putative NAD(P)-binding C-N hydrolase family amidase		_____________211____________________234\n_____________\|______________________\|\n5'-GUC...GGUUU___________UUUU_______CCACU...CCG-3'\n______________GAAGAUUACGC____GCAGCAA\n______________:\|\|\|\|:\|\|\|:\|____:\|\|\|\|\|\|\n______________UUUCUGAUGUG____UGUCGUU\n3'-UUU...ACCCG______________________AAGCA...GCA-5'\n_____________\|______________________\|\n_____________79_____________________60\n	227 -- 233	61 -- 67	-20.0989 kcal/mol	5.01896 kcal/mol	3.25783 kcal/mol	211 234@LB@ \| \|@LB@5'-GUC...GGUUU UUUU CCACU...CCG-3'@LB@ GAAGAUUACGC GCAGCAA@LB@ :\|\|\|\|:\|\|\|:\| :\|\|\|\|\|\|@LB@ UUUCUGAUGUG UGUCGUU@LB@3'-UUU...ACCCG AAGCA...GCA-5'@LB@ \| \|@LB@ 79 60@LB@@LB@@LB@Energy -11.82 kcal/mol@LB@Hybridization Energy -20.0989 kcal/mol@LB@Unfolding Energy - mRNA 5.01896 kcal/mol@LB@Unfolding Energy - sRNA 3.25783 kcal/mol@LB@@LB@Position - mRNA 212 -- 233@LB@Position - sRNA 61 -- 78@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 61 -- 67@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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