Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
192	0.00979	0.1776	b3879	yihR	-14.50	0.028125	239 -- 282	38 -- 72	putative sulphoquinovose mutarotase	_____________238__________________________________________283\n_____________\|____________________________________________\|\n5'-CAU...ACCAA_______U_____GGACAGACC________UGCCG_________AUCAC...UGA-3'\n______________UAUGCAU_GCAGU_________GUUUCACU_____CGGGGGAUU\n______________\|\|::\|\|:_\|\|\|:\|_________\|\|\|\|\|\|\|\|_____\|:\|\|\|\|\|\|\|\n______________AUGUGUG_CGUUA_________CAAAGUGA_____GUCCCCUAA\n3'-UUU...UUCUG_______U_____AGCAA__________________________AGGUA...GCA-5'\n_____________\|____________________________________________\|\n_____________73___________________________________________37\n	276 -- 282	38 -- 44	-32.397 kcal/mol	13.138 kcal/mol	4.76382 kcal/mol	238 283@LB@ \| \|@LB@5'-CAU...ACCAA U GGACAGACC UGCCG AUCAC...UGA-3'@LB@ UAUGCAU GCAGU GUUUCACU CGGGGGAUU@LB@ \|\|::\|\|: \|\|\|:\| \|\|\|\|\|\|\|\| \|:\|\|\|\|\|\|\|@LB@ AUGUGUG CGUUA CAAAGUGA GUCCCCUAA@LB@3'-UUU...UUCUG U AGCAA AGGUA...GCA-5'@LB@ \| \|@LB@ 73 37@LB@@LB@@LB@Energy -14.50 kcal/mol@LB@Hybridization Energy -32.397 kcal/mol@LB@Unfolding Energy - mRNA 13.138 kcal/mol@LB@Unfolding Energy - sRNA 4.76382 kcal/mol@LB@@LB@Position - mRNA 239 -- 282@LB@Position - sRNA 38 -- 72@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 38 -- 44@LB@
30	0.0004324	0.05073	b3364	tsgA	-16.38	0.008733	203 -- 236	38 -- 75	putative transporter	_____________202_____________________________________237\n_____________\|_______________________________________\|\n5'-CCA...AUCAU___________________CAUCAA______CAU_____AGCUU...AUA-3'\n______________GACUA___ACAGCAAU_CG_______GCUCA___GGAUU\n______________\|\|\|\|\|___\|\|\|\|\|\|\|\|_\|\|_______:\|\|\|\|___\|\|\|\|\|\n______________CUGAU___UGUCGUUA_GC_______UGAGU___CCUAA\n3'-UUU...CGUUU_____GUG________A__AACAAAG_____CC______AGGUA...GCA-5'\n_____________\|_______________________________________\|\n_____________76______________________________________37\n	209 -- 215	60 -- 66	-26.4683 kcal/mol	5.30565 kcal/mol	4.77946 kcal/mol	202 237@LB@ \| \|@LB@5'-CCA...AUCAU CAUCAA CAU AGCUU...AUA-3'@LB@ GACUA ACAGCAAU CG GCUCA GGAUU@LB@ \|\|\|\|\| \|\|\|\|\|\|\|\| \|\| :\|\|\|\| \|\|\|\|\|@LB@ CUGAU UGUCGUUA GC UGAGU CCUAA@LB@3'-UUU...CGUUU GUG A AACAAAG CC AGGUA...GCA-5'@LB@ \| \|@LB@ 76 37@LB@@LB@@LB@Energy -16.38 kcal/mol@LB@Hybridization Energy -26.4683 kcal/mol@LB@Unfolding Energy - mRNA 5.30565 kcal/mol@LB@Unfolding Energy - sRNA 4.77946 kcal/mol@LB@@LB@Position - mRNA 203 -- 236@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 60 -- 66@LB@
181	0.009071	0.1747	b3962	sthA	-14.30	0.031527	83 -- 114	34 -- 76	pyridine nucleotide transhydrogenase soluble	_____________82___________________________________________115\n_____________\|____________________________________________\|\n5'-UAC...GAAAC____AAA___AA_______________________AU__AG___GAUAA...CAG-3'\n______________AGAC___GCA__GGC____________CGCUCAGG__AU__CCA\n______________\|\|\|\|___:\|\|__:\|\|____________\|:\|\|\|\|\|\|__\|\|__\|\|\|\n______________UCUG___UGU__UCG____________GUGAGUCC__UA__GGU\n3'-UUU...CCGUU____AUG___G____UUAAGCAACAAA________CC__AA___ACGAA...GCA-5'\n_____________\|____________________________________________\|\n_____________77___________________________________________33\n	99 -- 105	43 -- 49	-23.8174 kcal/mol	4.70621 kcal/mol	4.816 kcal/mol	82 115@LB@ \| \|@LB@5'-UAC...GAAAC AAA AA AU AG GAUAA...CAG-3'@LB@ AGAC GCA GGC CGCUCAGG AU CCA@LB@ \|\|\|\| :\|\| :\|\| \|:\|\|\|\|\|\| \|\| \|\|\|@LB@ UCUG UGU UCG GUGAGUCC UA GGU@LB@3'-UUU...CCGUU AUG G UUAAGCAACAAA CC AA ACGAA...GCA-5'@LB@ \| \|@LB@ 77 33@LB@@LB@@LB@Energy -14.30 kcal/mol@LB@Hybridization Energy -23.8174 kcal/mol@LB@Unfolding Energy - mRNA 4.70621 kcal/mol@LB@Unfolding Energy - sRNA 4.816 kcal/mol@LB@@LB@Position - mRNA 83 -- 114@LB@Position - sRNA 34 -- 76@LB@Position Seed - mRNA 99 -- 105@LB@Position Seed - sRNA 43 -- 49@LB@
75	0.003119	0.1452	b0871	poxB	-11.12	0.154817	181 -- 203	32 -- 50	pyruvate dehydrogenase thiamine triphosphate-binding FAD-binding	_____________180_____________________204\n_____________\|_______________________\|\n5'-CCG...UAUCA________AGAU___GAA_____AAACA...GUG-3'\n______________CAUUCAGG____GGA___CCAUG\n______________\|\|:\|\|\|\|\|____\|\|\|___\|\|\|\|\|\n______________GUGAGUCC____CCU___GGUAC\n3'-UUU...ACAAA_______________AAA_____GAAUA...GCA-5'\n_____________\|_______________________\|\n_____________51______________________31\n	182 -- 188	43 -- 49	-21.2585 kcal/mol	5.84423 kcal/mol	4.29868 kcal/mol	180 204@LB@ \| \|@LB@5'-CCG...UAUCA AGAU GAA AAACA...GUG-3'@LB@ CAUUCAGG GGA CCAUG@LB@ \|\|:\|\|\|\|\| \|\|\| \|\|\|\|\|@LB@ GUGAGUCC CCU GGUAC@LB@3'-UUU...ACAAA AAA GAAUA...GCA-5'@LB@ \| \|@LB@ 51 31@LB@@LB@@LB@Energy -11.12 kcal/mol@LB@Hybridization Energy -21.2585 kcal/mol@LB@Unfolding Energy - mRNA 5.84423 kcal/mol@LB@Unfolding Energy - sRNA 4.29868 kcal/mol@LB@@LB@Position - mRNA 181 -- 203@LB@Position - sRNA 32 -- 50@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 43 -- 49@LB@
17	0.0001292	0.02674	b1854	pykA	-13.69	0.043994	133 -- 157	36 -- 68	pyruvate kinase II	_____________132_______________________________158\n_____________\|_________________________________\|\n5'-GAA...GCCAU______________A__________________AUGUU...ACG-3'\n______________CGCGGC_____GUU_UUUCAUUC___GGAUUUC\n______________\|:\|:\|\|_____\|\|\|_\|\|\|\|\|:\|\|___\|\|\|\|\|\|\|\n______________GUGUCG_____CAA_AAAGUGAG___CCUAAAG\n3'-UUU...GAUGU______UUAAG___C________UCC_______GUACG...GCA-5'\n_____________\|_________________________________\|\n_____________69________________________________35\n	151 -- 157	36 -- 42	-24.2432 kcal/mol	5.79946 kcal/mol	4.75078 kcal/mol	132 158@LB@ \| \|@LB@5'-GAA...GCCAU A AUGUU...ACG-3'@LB@ CGCGGC GUU UUUCAUUC GGAUUUC@LB@ \|:\|:\|\| \|\|\| \|\|\|\|\|:\|\| \|\|\|\|\|\|\|@LB@ GUGUCG CAA AAAGUGAG CCUAAAG@LB@3'-UUU...GAUGU UUAAG C UCC GUACG...GCA-5'@LB@ \| \|@LB@ 69 35@LB@@LB@@LB@Energy -13.69 kcal/mol@LB@Hybridization Energy -24.2432 kcal/mol@LB@Unfolding Energy - mRNA 5.79946 kcal/mol@LB@Unfolding Energy - sRNA 4.75078 kcal/mol@LB@@LB@Position - mRNA 133 -- 157@LB@Position - sRNA 36 -- 68@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 36 -- 42@LB@
41	0.0008963	0.07693	b1378	pfo	-13.27	0.055135	136 -- 164	58 -- 78	pyruvate-flavodoxin oxidoreductase	_____________135___________________________165\n_____________\|_____________________________\|\n5'-GCU...AAUUG_________UACUGGGC____________CGUGC...CAG-3'\n______________AAAGAUUAC________GCGCAGCAAUUU\n______________\|\|\|\|\|:\|\|\|________:\|:\|\|\|\|\|\|\|\|:\n______________UUUCUGAUG________UGUGUCGUUAAG\n3'-UUU...ACCCG_____________________________CAACA...GCA-5'\n_____________\|_____________________________\|\n_____________79____________________________57\n	158 -- 164	58 -- 64	-22.0871 kcal/mol	5.50788 kcal/mol	3.31018 kcal/mol	135 165@LB@ \| \|@LB@5'-GCU...AAUUG UACUGGGC CGUGC...CAG-3'@LB@ AAAGAUUAC GCGCAGCAAUUU@LB@ \|\|\|\|\|:\|\|\| :\|:\|\|\|\|\|\|\|\|:@LB@ UUUCUGAUG UGUGUCGUUAAG@LB@3'-UUU...ACCCG CAACA...GCA-5'@LB@ \| \|@LB@ 79 57@LB@@LB@@LB@Energy -13.27 kcal/mol@LB@Hybridization Energy -22.0871 kcal/mol@LB@Unfolding Energy - mRNA 5.50788 kcal/mol@LB@Unfolding Energy - sRNA 3.31018 kcal/mol@LB@@LB@Position - mRNA 136 -- 164@LB@Position - sRNA 58 -- 78@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 58 -- 64@LB@
14	0.0001025	0.0245	b4051	qorA	-14.73	0.024599	192 -- 224	48 -- 76	quinone oxidoreductase NADPH-dependent	_____________191_______________________________225\n_____________\|_________________________________\|\n5'-CGC...GUUCU___GG___________CA__AA___AA______AAGCA...UCG-3'\n______________GGA__ACACAUGGCAA__CG__UUG__UUUCAC\n______________:\|\|__\|\|\|\|\|::\|\|\|\|__\|\|__\|\|\|__\|\|\|\|\|\|\n______________UCU__UGUGUGUCGUU__GC__AAC__AAAGUG\n3'-UUU...CCGUU___GA___________AA_______________AGUCC...GCA-5'\n_____________\|_________________________________\|\n_____________77________________________________47\n	201 -- 207	61 -- 67	-23.6874 kcal/mol	5.27405 kcal/mol	3.68714 kcal/mol	191 225@LB@ \| \|@LB@5'-CGC...GUUCU GG CA AA AA AAGCA...UCG-3'@LB@ GGA ACACAUGGCAA CG UUG UUUCAC@LB@ :\|\| \|\|\|\|\|::\|\|\|\| \|\| \|\|\| \|\|\|\|\|\|@LB@ UCU UGUGUGUCGUU GC AAC AAAGUG@LB@3'-UUU...CCGUU GA AA AGUCC...GCA-5'@LB@ \| \|@LB@ 77 47@LB@@LB@@LB@Energy -14.73 kcal/mol@LB@Hybridization Energy -23.6874 kcal/mol@LB@Unfolding Energy - mRNA 5.27405 kcal/mol@LB@Unfolding Energy - sRNA 3.68714 kcal/mol@LB@@LB@Position - mRNA 192 -- 224@LB@Position - sRNA 48 -- 76@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 61 -- 67@LB@
179	0.009001	0.1747	b2725	hycA	-11.58	0.125812	239 -- 264	43 -- 70	regulator of the transcriptional regulator FhlA	_____________238_____________________________265\n_____________\|_______________________________\|\n5'-UUA...UACAU__________CA____________CUA____ACCAG...AGG-3'\n______________CGCACAGCGG__UCG_____UCGC___CAGG\n______________\|:\|\|\|\|\|\|::__\|\|\|_____\|\|:\|___\|\|\|\|\n______________GUGUGUCGUU__AGC_____AGUG___GUCC\n3'-UUU...CUGAU__________A____AACAA____A______CCUAA...GCA-5'\n_____________\|_______________________________\|\n_____________71______________________________42\n	242 -- 248	61 -- 67	-23.0547 kcal/mol	7.15003 kcal/mol	4.32399 kcal/mol	238 265@LB@ \| \|@LB@5'-UUA...UACAU CA CUA ACCAG...AGG-3'@LB@ CGCACAGCGG UCG UCGC CAGG@LB@ \|:\|\|\|\|\|\|:: \|\|\| \|\|:\| \|\|\|\|@LB@ GUGUGUCGUU AGC AGUG GUCC@LB@3'-UUU...CUGAU A AACAA A CCUAA...GCA-5'@LB@ \| \|@LB@ 71 42@LB@@LB@@LB@Energy -11.58 kcal/mol@LB@Hybridization Energy -23.0547 kcal/mol@LB@Unfolding Energy - mRNA 7.15003 kcal/mol@LB@Unfolding Energy - sRNA 4.32399 kcal/mol@LB@@LB@Position - mRNA 239 -- 264@LB@Position - sRNA 43 -- 70@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 61 -- 67@LB@
60	0.002324	0.1363	b0881	clpS	-11.74	0.117068	9 -- 15	40 -- 46	regulatory protein for ClpA substrate specificity	_____________8_______16\n_____________\|_______\|\n___5'-GCAUUUGA_______CAAGG...UCA-3'\n______________CAGGGGA\n______________\|\|\|\|\|\|\|\n______________GUCCCCU\n3'-UUU...AGUGA_______AAAGG...GCA-5'\n_____________\|_______\|\n_____________47______39\n	9 -- 15	40 -- 46	-13.2936 kcal/mol	0.199257 kcal/mol	1.35582 kcal/mol	8 16@LB@ \| \|@LB@ 5'-GCAUUUGA CAAGG...UCA-3'@LB@ CAGGGGA@LB@ \|\|\|\|\|\|\|@LB@ GUCCCCU@LB@3'-UUU...AGUGA AAAGG...GCA-5'@LB@ \| \|@LB@ 47 39@LB@@LB@@LB@Energy -11.74 kcal/mol@LB@Hybridization Energy -13.2936 kcal/mol@LB@Unfolding Energy - mRNA 0.199257 kcal/mol@LB@Unfolding Energy - sRNA 1.35582 kcal/mol@LB@@LB@Position - mRNA 9 -- 15@LB@Position - sRNA 40 -- 46@LB@Position Seed - mRNA 9 -- 15@LB@Position Seed - sRNA 40 -- 46@LB@
111	0.004903	0.1545	b1735	chbR	-11.17	0.151186	109 -- 138	30 -- 50	repressor of chb operon for N N'-diacetylchitobiose utilization	_____________108____________________________139\n_____________\|______________________________\|\n5'-GAU...GUCCA________AUCAUUUAAU__CG________UGCGC...CUG-3'\n______________CGCUCAGG__________GA__UCCAUGCU\n______________\|:\|\|\|\|\|\|__________\|\|__\|\|\|\|\|\|\|\|\n______________GUGAGUCC__________CU__AGGUACGA\n3'-UUU...ACAAA________C___________AA________AUAUU...GCA-5'\n_____________\|______________________________\|\n_____________51_____________________________29\n	132 -- 138	30 -- 36	-23.5874 kcal/mol	7.28235 kcal/mol	5.13509 kcal/mol	108 139@LB@ \| \|@LB@5'-GAU...GUCCA AUCAUUUAAU CG UGCGC...CUG-3'@LB@ CGCUCAGG GA UCCAUGCU@LB@ \|:\|\|\|\|\|\| \|\| \|\|\|\|\|\|\|\|@LB@ GUGAGUCC CU AGGUACGA@LB@3'-UUU...ACAAA C AA AUAUU...GCA-5'@LB@ \| \|@LB@ 51 29@LB@@LB@@LB@Energy -11.17 kcal/mol@LB@Hybridization Energy -23.5874 kcal/mol@LB@Unfolding Energy - mRNA 7.28235 kcal/mol@LB@Unfolding Energy - sRNA 5.13509 kcal/mol@LB@@LB@Position - mRNA 109 -- 138@LB@Position - sRNA 30 -- 50@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 30 -- 36@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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