Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
159	0.0318	0.6177	b2532	trmJ	-7.64	0.122614	101 -- 114	44 -- 55	tRNA mC32 mU32 2'-O-methyltransferase SAM-dependent	_____________100____________115\n_____________\|______________\|\n5'-CGU...UGGAU________AC____AAACG...UGU-3'\n______________GUUAAAGA__GGGA\n______________\|\|:\|\|\|\|\|__\|\|:\|\n______________CAGUUUCU__CCUU\n3'-UUU...CCGGG______________AAAGU...ACA-5'\n_____________\|______________\|\n_____________56_____________43\n	102 -- 108	48 -- 54	-10.9175 kcal/mol	2.33739 kcal/mol	0.941432 kcal/mol	100 115@LB@ \| \|@LB@5'-CGU...UGGAU AC AAACG...UGU-3'@LB@ GUUAAAGA GGGA@LB@ \|\|:\|\|\|\|\| \|\|:\|@LB@ CAGUUUCU CCUU@LB@3'-UUU...CCGGG AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -7.64 kcal/mol@LB@Hybridization Energy -10.9175 kcal/mol@LB@Unfolding Energy - mRNA 2.33739 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 101 -- 114@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 48 -- 54@LB@
195	0.04073	0.6177	b3166	truB	-9.24	0.058593	189 -- 197	45 -- 53	tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase	_____________188_______198\n_____________\|_________\|\n5'-GGC...GACAG_________CUAAU...AAC-3'\n______________CAAGGAGGA\n______________\|\|\|:\|\|\|\|\|\n______________GUUUCUCCU\n3'-UUU...GGGCA_________UAAAG...ACA-5'\n_____________\|_________\|\n_____________54________44\n	191 -- 197	45 -- 51	-13.1895 kcal/mol	3.02734 kcal/mol	0.917553 kcal/mol	188 198@LB@ \| \|@LB@5'-GGC...GACAG CUAAU...AAC-3'@LB@ CAAGGAGGA@LB@ \|\|\|:\|\|\|\|\|@LB@ GUUUCUCCU@LB@3'-UUU...GGGCA UAAAG...ACA-5'@LB@ \| \|@LB@ 54 44@LB@@LB@@LB@Energy -9.24 kcal/mol@LB@Hybridization Energy -13.1895 kcal/mol@LB@Unfolding Energy - mRNA 3.02734 kcal/mol@LB@Unfolding Energy - sRNA 0.917553 kcal/mol@LB@@LB@Position - mRNA 189 -- 197@LB@Position - sRNA 45 -- 53@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 45 -- 51@LB@
86	0.01794	0.6177	b3260	dusB	-7.53	0.128932	119 -- 138	38 -- 55	tRNA-dihydrouridine synthase B	_____________118__________________139\n_____________\|____________________\|\n5'-ACU...GAUUG_______UUU__CC______CUCGU...GAG-3'\n______________GUCAAAG___GG__UUUCAU\n______________\|\|\|\|\|\|\|___\|\|__\|\|\|\|\|\|\n______________CAGUUUC___CC__AAAGUA\n3'-UUU...CCGGG_______U____UU______AAAAA...ACA-5'\n_____________\|____________________\|\n_____________56___________________37\n	119 -- 125	49 -- 55	-13.3869 kcal/mol	4.6894 kcal/mol	1.17036 kcal/mol	118 139@LB@ \| \|@LB@5'-ACU...GAUUG UUU CC CUCGU...GAG-3'@LB@ GUCAAAG GG UUUCAU@LB@ \|\|\|\|\|\|\| \|\| \|\|\|\|\|\|@LB@ CAGUUUC CC AAAGUA@LB@3'-UUU...CCGGG U UU AAAAA...ACA-5'@LB@ \| \|@LB@ 56 37@LB@@LB@@LB@Energy -7.53 kcal/mol@LB@Hybridization Energy -13.3869 kcal/mol@LB@Unfolding Energy - mRNA 4.6894 kcal/mol@LB@Unfolding Energy - sRNA 1.17036 kcal/mol@LB@@LB@Position - mRNA 119 -- 138@LB@Position - sRNA 38 -- 55@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 49 -- 55@LB@
175	0.03585	0.6177	b2028	ugd	-9.30	0.056993	217 -- 248	46 -- 74	UDP-glucose 6-dehydrogenase	_____________216______________________________249\n_____________\|________________________________\|\n5'-GCU...CAUUU______C_G_____GUA___________C___CUUCU...CAC-3'\n______________CCGGUA_U_GCUAU___GGCUUGUCAAA_GGG\n______________\|\|::\|\|_\|_\|\|\|\|\|___\|\|\|::\|\|\|\|\|\|_:\|\|\n______________GGUUAU_A_CGAUA___CCGGGCAGUUU_UCC\n3'-UUU...UUACC________G_____A_____________C___UUAAA...ACA-5'\n_____________\|________________________________\|\n_____________75_______________________________45\n	238 -- 244	50 -- 56	-30.8605 kcal/mol	10.2215 kcal/mol	11.335 kcal/mol	216 249@LB@ \| \|@LB@5'-GCU...CAUUU C G GUA C CUUCU...CAC-3'@LB@ CCGGUA U GCUAU GGCUUGUCAAA GGG@LB@ \|\|::\|\| \| \|\|\|\|\| \|\|\|::\|\|\|\|\|\| :\|\|@LB@ GGUUAU A CGAUA CCGGGCAGUUU UCC@LB@3'-UUU...UUACC G A C UUAAA...ACA-5'@LB@ \| \|@LB@ 75 45@LB@@LB@@LB@Energy -9.30 kcal/mol@LB@Hybridization Energy -30.8605 kcal/mol@LB@Unfolding Energy - mRNA 10.2215 kcal/mol@LB@Unfolding Energy - sRNA 11.335 kcal/mol@LB@@LB@Position - mRNA 217 -- 248@LB@Position - sRNA 46 -- 74@LB@Position Seed - mRNA 238 -- 244@LB@Position Seed - sRNA 50 -- 56@LB@
155	0.03087	0.6177	b1003	yccJ	-9.03	0.064630	130 -- 145	43 -- 56	uncharacterized protein	_____________129______________146\n_____________\|________________\|\n5'-CCU...UUGCU_____UC_________AUGUC...GGU-3'\n______________CGUUA__AAGGGGAAU\n______________\|\|\|:\|__\|\|\|:\|\|\|\|\|\n______________GCAGU__UUCUCCUUA\n3'-UUU...ACCGG________________AAGUA...ACA-5'\n_____________\|________________\|\n_____________57_______________42\n	139 -- 145	43 -- 49	-13.8314 kcal/mol	3.84343 kcal/mol	0.953958 kcal/mol	129 146@LB@ \| \|@LB@5'-CCU...UUGCU UC AUGUC...GGU-3'@LB@ CGUUA AAGGGGAAU@LB@ \|\|\|:\| \|\|\|:\|\|\|\|\|@LB@ GCAGU UUCUCCUUA@LB@3'-UUU...ACCGG AAGUA...ACA-5'@LB@ \| \|@LB@ 57 42@LB@@LB@@LB@Energy -9.03 kcal/mol@LB@Hybridization Energy -13.8314 kcal/mol@LB@Unfolding Energy - mRNA 3.84343 kcal/mol@LB@Unfolding Energy - sRNA 0.953958 kcal/mol@LB@@LB@Position - mRNA 130 -- 145@LB@Position - sRNA 43 -- 56@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 43 -- 49@LB@
198	0.04148	0.6177	b1067	yceH	-6.24	0.226874	185 -- 202	38 -- 56	UPF0502 family protein	_____________184_________________203\n_____________\|___________________\|\n5'-GCA...CCGGC__C___________C____GAAAU...GUG-3'\n______________CG_UAAGGAG_AAU_UCAU\n______________\|\|_:\|\|:\|\|\|_\|\|\|_\|\|\|\|\n______________GC_GUUUCUC_UUA_AGUA\n3'-UUU...ACCGG__A_______C___A____AAAAA...ACA-5'\n_____________\|___________________\|\n_____________57__________________37\n	188 -- 194	47 -- 53	-10.8881 kcal/mol	3.47135 kcal/mol	1.17918 kcal/mol	184 203@LB@ \| \|@LB@5'-GCA...CCGGC C C GAAAU...GUG-3'@LB@ CG UAAGGAG AAU UCAU@LB@ \|\| :\|\|:\|\|\| \|\|\| \|\|\|\|@LB@ GC GUUUCUC UUA AGUA@LB@3'-UUU...ACCGG A C A AAAAA...ACA-5'@LB@ \| \|@LB@ 57 37@LB@@LB@@LB@Energy -6.24 kcal/mol@LB@Hybridization Energy -10.8881 kcal/mol@LB@Unfolding Energy - mRNA 3.47135 kcal/mol@LB@Unfolding Energy - sRNA 1.17918 kcal/mol@LB@@LB@Position - mRNA 185 -- 202@LB@Position - sRNA 38 -- 56@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 47 -- 53@LB@
80	0.01709	0.6177	b2498	upp	-4.70	0.418803	173 -- 195	44 -- 70	uracil phosphoribosyltransferase	_____________172_________________________196\n_____________\|___________________________\|\n5'-GGC...UUUUU___G___________C_______A___AGAGU...CCG-3'\n______________UGU_GCU____GCCC_UCAAAGG_GAA\n______________\|:\|_\|\|\|____\|\|\|\|_\|\|\|\|\|\|:_\|\|\|\n______________AUA_CGA____CGGG_AGUUUCU_CUU\n3'-UUU...CGGUU___G___UAAC____C_______C___AAAGU...ACA-5'\n_____________\|___________________________\|\n_____________71__________________________43\n	185 -- 191	48 -- 54	-22.3772 kcal/mol	6.37003 kcal/mol	11.3101 kcal/mol	172 196@LB@ \| \|@LB@5'-GGC...UUUUU G C A AGAGU...CCG-3'@LB@ UGU GCU GCCC UCAAAGG GAA@LB@ \|:\| \|\|\| \|\|\|\| \|\|\|\|\|\|: \|\|\|@LB@ AUA CGA CGGG AGUUUCU CUU@LB@3'-UUU...CGGUU G UAAC C C AAAGU...ACA-5'@LB@ \| \|@LB@ 71 43@LB@@LB@@LB@Energy -4.70 kcal/mol@LB@Hybridization Energy -22.3772 kcal/mol@LB@Unfolding Energy - mRNA 6.37003 kcal/mol@LB@Unfolding Energy - sRNA 11.3101 kcal/mol@LB@@LB@Position - mRNA 173 -- 195@LB@Position - sRNA 44 -- 70@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 48 -- 54@LB@
29	0.005664	0.6127	b3831	udp	-8.88	0.069383	184 -- 196	42 -- 54	uridine phosphorylase	_____________183___________197\n_____________\|_____________\|\n5'-AUC...CCGAU___C_________GUAUA...AGA-3'\n______________UCA_AGAGGAGUU\n______________\|\|\|_\|\|\|\|\|\|:\|\|\n______________AGU_UCUCCUUAA\n3'-UUU...CGGGC___U_________AGUAA...ACA-5'\n_____________\|_____________\|\n_____________55____________41\n	190 -- 196	42 -- 48	-14.4874 kcal/mol	4.66075 kcal/mol	0.945538 kcal/mol	183 197@LB@ \| \|@LB@5'-AUC...CCGAU C GUAUA...AGA-3'@LB@ UCA AGAGGAGUU@LB@ \|\|\| \|\|\|\|\|\|:\|\|@LB@ AGU UCUCCUUAA@LB@3'-UUU...CGGGC U AGUAA...ACA-5'@LB@ \| \|@LB@ 55 41@LB@@LB@@LB@Energy -8.88 kcal/mol@LB@Hybridization Energy -14.4874 kcal/mol@LB@Unfolding Energy - mRNA 4.66075 kcal/mol@LB@Unfolding Energy - sRNA 0.945538 kcal/mol@LB@@LB@Position - mRNA 184 -- 196@LB@Position - sRNA 42 -- 54@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 42 -- 48@LB@
34	0.007003	0.6129	b4199	yjfY	-12.43	0.013073	184 -- 195	46 -- 57	YhcN family protein periplasmic	_____________183__________196\n_____________\|____________\|\n5'-AGG...GUCAG____________UAUCU...ACG-3'\n______________CCGUCGAGGAGG\n______________\|\|\|\|\|:\|:\|\|\|\|\n______________GGCAGUUUCUCC\n3'-UUU...AACCG____________UUAAA...ACA-5'\n_____________\|____________\|\n_____________58___________45\n	189 -- 195	46 -- 52	-19.9606 kcal/mol	4.79886 kcal/mol	2.7365 kcal/mol	183 196@LB@ \| \|@LB@5'-AGG...GUCAG UAUCU...ACG-3'@LB@ CCGUCGAGGAGG@LB@ \|\|\|\|\|:\|:\|\|\|\|@LB@ GGCAGUUUCUCC@LB@3'-UUU...AACCG UUAAA...ACA-5'@LB@ \| \|@LB@ 58 45@LB@@LB@@LB@Energy -12.43 kcal/mol@LB@Hybridization Energy -19.9606 kcal/mol@LB@Unfolding Energy - mRNA 4.79886 kcal/mol@LB@Unfolding Energy - sRNA 2.7365 kcal/mol@LB@@LB@Position - mRNA 184 -- 195@LB@Position - sRNA 46 -- 57@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 46 -- 52@LB@
6	0.0006545	0.351	b1857	znuA	-11.78	0.017745	58 -- 67	45 -- 54	zinc ABC transporter periplasmic binding protein	_____________57_________68\n_____________\|__________\|\n5'-GCG...ACACA__________CGCGG...AAC-3'\n______________UCAGAGAGGA\n______________\|\|\|:\|\|\|\|\|\|\n______________AGUUUCUCCU\n3'-UUU...CGGGC__________UAAAG...ACA-5'\n_____________\|__________\|\n_____________55_________44\n	61 -- 67	45 -- 51	-14.7441 kcal/mol	2.03423 kcal/mol	0.929144 kcal/mol	57 68@LB@ \| \|@LB@5'-GCG...ACACA CGCGG...AAC-3'@LB@ UCAGAGAGGA@LB@ \|\|\|:\|\|\|\|\|\|@LB@ AGUUUCUCCU@LB@3'-UUU...CGGGC UAAAG...ACA-5'@LB@ \| \|@LB@ 55 44@LB@@LB@@LB@Energy -11.78 kcal/mol@LB@Hybridization Energy -14.7441 kcal/mol@LB@Unfolding Energy - mRNA 2.03423 kcal/mol@LB@Unfolding Energy - sRNA 0.929144 kcal/mol@LB@@LB@Position - mRNA 58 -- 67@LB@Position - sRNA 45 -- 54@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 45 -- 51@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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