Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
109	0.02227	0.6177	b4040	ubiA	-8.97	0.066674	182 -- 194	44 -- 55	p-hydroxybenzoate octaprenyltransferase	_____________181___________195\n_____________\|_____________\|\n5'-AGC...CCGUU__A_U________AAAAA...UAU-3'\n______________GU_C_AAGAGGAA\n______________\|\|_\|_\|\|\|\|\|\|\|\|\n______________CA_G_UUCUCCUU\n3'-UUU...CCGGG____U________AAAGU...ACA-5'\n_____________\|_____________\|\n_____________56____________43\n	188 -- 194	44 -- 50	-10.7786 kcal/mol	0.870226 kcal/mol	0.941432 kcal/mol	181 195@LB@ \| \|@LB@5'-AGC...CCGUU A U AAAAA...UAU-3'@LB@ GU C AAGAGGAA@LB@ \|\| \| \|\|\|\|\|\|\|\|@LB@ CA G UUCUCCUU@LB@3'-UUU...CCGGG U AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -8.97 kcal/mol@LB@Hybridization Energy -10.7786 kcal/mol@LB@Unfolding Energy - mRNA 0.870226 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 182 -- 194@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 44 -- 50@LB@
99	0.01953	0.6177	b2473	ypfH	-12.09	0.015349	8 -- 40	44 -- 76	palmitoyl-CoA esterase activity uncertain physiological substrate	_____________7__________________________________41\n_____________\|__________________________________\|\n____5'-ACUUUCA_____CAAGA_____C___________C______GAGGC...GGG-3'\n______________GGCCG_____UGUUA_UGGUCCGUCAG_G_GGAA\n______________\|\|\|\|:_____:\|:\|\|_\|\|\|:\|\|\|\|\|\|:_\|_\|\|\|\|\n______________CCGGU_____GCGAU_ACCGGGCAGUU_C_CCUU\n___3'-UUUUUUUA_____UAUA______A___________U_U____AAAGU...ACA-5'\n_____________\|__________________________________\|\n_____________77_________________________________43\n	28 -- 34	51 -- 57	-34.8713 kcal/mol	9.35151 kcal/mol	13.433 kcal/mol	7 41@LB@ \| \|@LB@ 5'-ACUUUCA CAAGA C C GAGGC...GGG-3'@LB@ GGCCG UGUUA UGGUCCGUCAG G GGAA@LB@ \|\|\|\|: :\|:\|\| \|\|\|:\|\|\|\|\|\|: \| \|\|\|\|@LB@ CCGGU GCGAU ACCGGGCAGUU C CCUU@LB@ 3'-UUUUUUUA UAUA A U U AAAGU...ACA-5'@LB@ \| \|@LB@ 77 43@LB@@LB@@LB@Energy -12.09 kcal/mol@LB@Hybridization Energy -34.8713 kcal/mol@LB@Unfolding Energy - mRNA 9.35151 kcal/mol@LB@Unfolding Energy - sRNA 13.433 kcal/mol@LB@@LB@Position - mRNA 8 -- 40@LB@Position - sRNA 44 -- 76@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 51 -- 57@LB@
163	0.03345	0.6177	b1235	rssB	-5.20	0.346272	186 -- 195	44 -- 55	PcnB-degradosome interaction factor; response regulator	_____________185__________196\n_____________\|____________\|\n5'-AAU...AGCCA____________AGAAC...CAA-3'\n______________GUC__AGGGGAG\n______________\|\|\|__\|\|:\|\|\|:\n______________CAG__UCUCCUU\n3'-UUU...CCGGG___UU_______AAAGU...ACA-5'\n_____________\|____________\|\n_____________56___________43\n	189 -- 195	44 -- 50	-10.1709 kcal/mol	4.02871 kcal/mol	0.941432 kcal/mol	185 196@LB@ \| \|@LB@5'-AAU...AGCCA AGAAC...CAA-3'@LB@ GUC AGGGGAG@LB@ \|\|\| \|\|:\|\|\|:@LB@ CAG UCUCCUU@LB@3'-UUU...CCGGG UU AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -5.20 kcal/mol@LB@Hybridization Energy -10.1709 kcal/mol@LB@Unfolding Energy - mRNA 4.02871 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 186 -- 195@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 44 -- 50@LB@
55	0.01149	0.6177	b3287	def	-9.61	0.049470	133 -- 146	41 -- 54	peptide deformylase	_____________132____________147\n_____________\|______________\|\n5'-AUA...GUCAA______________GUCUA...AUG-3'\n______________UCAGAGGGGGAUUU\n______________\|\|\|:\|\|:\|\|:\|\|\|\|\n______________AGUUUCUCCUUAAA\n3'-UUU...CGGGC______________GUAAA...ACA-5'\n_____________\|______________\|\n_____________55_____________40\n	140 -- 146	41 -- 47	-17.2173 kcal/mol	6.66439 kcal/mol	0.946064 kcal/mol	132 147@LB@ \| \|@LB@5'-AUA...GUCAA GUCUA...AUG-3'@LB@ UCAGAGGGGGAUUU@LB@ \|\|\|:\|\|:\|\|:\|\|\|\|@LB@ AGUUUCUCCUUAAA@LB@3'-UUU...CGGGC GUAAA...ACA-5'@LB@ \| \|@LB@ 55 40@LB@@LB@@LB@Energy -9.61 kcal/mol@LB@Hybridization Energy -17.2173 kcal/mol@LB@Unfolding Energy - mRNA 6.66439 kcal/mol@LB@Unfolding Energy - sRNA 0.946064 kcal/mol@LB@@LB@Position - mRNA 133 -- 146@LB@Position - sRNA 41 -- 54@LB@Position Seed - mRNA 140 -- 146@LB@Position Seed - sRNA 41 -- 47@LB@
92	0.01873	0.6177	b2297	pta	-7.94	0.106741	187 -- 219	22 -- 52	phosphate acetyltransferase	_____________186_______________________________220\n_____________\|_________________________________\|\n5'-CGC...UAACG________UAAAC___GUCCCG___________UGAUC...GUC-3'\n______________AAAGAGGA_____CGU______UAUUAUUAUGC\n______________\|\|\|\|\|\|\|\|_____\|:\|______\|\|\|\|\|\|\|\|\|\|\|\n______________UUUCUCCU_____GUA______AUAAUAAUACG\n3'-UUU...GGCAG________UAAA____AAAAA____________GCAGU...ACA-5'\n_____________\|_________________________________\|\n_____________53________________________________21\n	213 -- 219	22 -- 28	-21.7034 kcal/mol	9.76868 kcal/mol	3.9903 kcal/mol	186 220@LB@ \| \|@LB@5'-CGC...UAACG UAAAC GUCCCG UGAUC...GUC-3'@LB@ AAAGAGGA CGU UAUUAUUAUGC@LB@ \|\|\|\|\|\|\|\| \|:\| \|\|\|\|\|\|\|\|\|\|\|@LB@ UUUCUCCU GUA AUAAUAAUACG@LB@3'-UUU...GGCAG UAAA AAAAA GCAGU...ACA-5'@LB@ \| \|@LB@ 53 21@LB@@LB@@LB@Energy -7.94 kcal/mol@LB@Hybridization Energy -21.7034 kcal/mol@LB@Unfolding Energy - mRNA 9.76868 kcal/mol@LB@Unfolding Energy - sRNA 3.9903 kcal/mol@LB@@LB@Position - mRNA 187 -- 219@LB@Position - sRNA 22 -- 52@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 22 -- 28@LB@
32	0.006131	0.6129	b2585	pssA	-9.74	0.046374	32 -- 41	45 -- 54	phosphatidylserine synthase; CDP-diacylglycerol-serine O-phosphatidyltransferase	_____________31_________42\n_____________\|__________\|\n5'-CGC...CCAGC__________GAUCG...CCG-3'\n______________UCGAAGAGGG\n______________\|\|:\|\|\|\|\|\|:\n______________AGUUUCUCCU\n3'-UUU...CGGGC__________UAAAG...ACA-5'\n_____________\|__________\|\n_____________55_________44\n	35 -- 41	45 -- 51	-13.2862 kcal/mol	2.61225 kcal/mol	0.929144 kcal/mol	31 42@LB@ \| \|@LB@5'-CGC...CCAGC GAUCG...CCG-3'@LB@ UCGAAGAGGG@LB@ \|\|:\|\|\|\|\|\|:@LB@ AGUUUCUCCU@LB@3'-UUU...CGGGC UAAAG...ACA-5'@LB@ \| \|@LB@ 55 44@LB@@LB@@LB@Energy -9.74 kcal/mol@LB@Hybridization Energy -13.2862 kcal/mol@LB@Unfolding Energy - mRNA 2.61225 kcal/mol@LB@Unfolding Energy - sRNA 0.929144 kcal/mol@LB@@LB@Position - mRNA 32 -- 41@LB@Position - sRNA 45 -- 54@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 45 -- 51@LB@
22	0.004195	0.5992	b3715	yieH	-7.44	0.134211	287 -- 299	41 -- 55	phosphoenolpyruvate and 6-phosphogluconate phosphatase	_____________286_____________300\n_____________\|_______________\|\n5'-CUG...ACGAU___UC__________G-3'\n______________GUU____AGGAAUUU\n______________\|\|:____\|\|\|\|\|\|\|\|\n______________CAG____UCCUUAAA\n3'-UUU...CCGGG___UUUC________GUAAA...ACA-5'\n_____________\|_______________\|\n_____________56______________40\n	293 -- 299	41 -- 47	-8.9861 kcal/mol	0.60049 kcal/mol	0.947844 kcal/mol	286 300@LB@ \| \|@LB@5'-CUG...ACGAU UC G-3'@LB@ GUU AGGAAUUU@LB@ \|\|: \|\|\|\|\|\|\|\|@LB@ CAG UCCUUAAA@LB@3'-UUU...CCGGG UUUC GUAAA...ACA-5'@LB@ \| \|@LB@ 56 40@LB@@LB@@LB@Energy -7.44 kcal/mol@LB@Hybridization Energy -8.9861 kcal/mol@LB@Unfolding Energy - mRNA 0.60049 kcal/mol@LB@Unfolding Energy - sRNA 0.947844 kcal/mol@LB@@LB@Position - mRNA 287 -- 299@LB@Position - sRNA 41 -- 55@LB@Position Seed - mRNA 293 -- 299@LB@Position Seed - sRNA 41 -- 47@LB@
65	0.01408	0.6177	b3176	glmM	-9.74	0.046438	133 -- 141	48 -- 56	phosphoglucosamine mutase	_____________132_______142\n_____________\|_________\|\n5'-GUU...CAUGA_________AACCG...CGG-3'\n______________CGUCAAAGA\n______________\|\|\|\|\|\|\|\|\|\n______________GCAGUUUCU\n3'-UUU...ACCGG_________CCUUA...ACA-5'\n_____________\|_________\|\n_____________57________47\n	135 -- 141	48 -- 54	-11.8395 kcal/mol	1.20122 kcal/mol	0.896433 kcal/mol	132 142@LB@ \| \|@LB@5'-GUU...CAUGA AACCG...CGG-3'@LB@ CGUCAAAGA@LB@ \|\|\|\|\|\|\|\|\|@LB@ GCAGUUUCU@LB@3'-UUU...ACCGG CCUUA...ACA-5'@LB@ \| \|@LB@ 57 47@LB@@LB@@LB@Energy -9.74 kcal/mol@LB@Hybridization Energy -11.8395 kcal/mol@LB@Unfolding Energy - mRNA 1.20122 kcal/mol@LB@Unfolding Energy - sRNA 0.896433 kcal/mol@LB@@LB@Position - mRNA 133 -- 141@LB@Position - sRNA 48 -- 56@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 48 -- 54@LB@
94	0.01884	0.6177	b2048	cpsG	-10.80	0.028164	47 -- 56	45 -- 54	phosphomannomutase	_____________46_________57\n_____________\|__________\|\n5'-GGG...UUAUC__________UGAUG...GCG-3'\n______________UCGAAGAGGA\n______________\|\|:\|\|\|\|\|\|\|\n______________AGUUUCUCCU\n3'-UUU...CGGGC__________UAAAG...ACA-5'\n_____________\|__________\|\n_____________55_________44\n	50 -- 56	45 -- 51	-14.144 kcal/mol	2.41054 kcal/mol	0.929144 kcal/mol	46 57@LB@ \| \|@LB@5'-GGG...UUAUC UGAUG...GCG-3'@LB@ UCGAAGAGGA@LB@ \|\|:\|\|\|\|\|\|\|@LB@ AGUUUCUCCU@LB@3'-UUU...CGGGC UAAAG...ACA-5'@LB@ \| \|@LB@ 55 44@LB@@LB@@LB@Energy -10.80 kcal/mol@LB@Hybridization Energy -14.144 kcal/mol@LB@Unfolding Energy - mRNA 2.41054 kcal/mol@LB@Unfolding Energy - sRNA 0.929144 kcal/mol@LB@@LB@Position - mRNA 47 -- 56@LB@Position - sRNA 45 -- 54@LB@Position Seed - mRNA 50 -- 56@LB@Position Seed - sRNA 45 -- 51@LB@
76	0.01599	0.6177	b1849	purT	-9.18	0.060248	187 -- 212	25 -- 50	phosphoribosylglycinamide formyltransferase 2	_____________186________________________213\n_____________\|__________________________\|\n5'-UUU...UAAUC______UA______AUGAC_______GGCAC...GUC-3'\n______________AGGGGA__UUCGUU_____GUUAUUA\n______________\|\|:\|\|\|__\|\|\|:\|\|_____:\|\|\|\|\|\|\n______________UCUCCU__AAGUAA_____UAAUAAU\n3'-UUU...CAGUU______UA______AAAAA_______ACGGC...ACA-5'\n_____________\|__________________________\|\n_____________51_________________________24\n	206 -- 212	25 -- 31	-15.1619 kcal/mol	4.88468 kcal/mol	1.09239 kcal/mol	186 213@LB@ \| \|@LB@5'-UUU...UAAUC UA AUGAC GGCAC...GUC-3'@LB@ AGGGGA UUCGUU GUUAUUA@LB@ \|\|:\|\|\| \|\|\|:\|\| :\|\|\|\|\|\|@LB@ UCUCCU AAGUAA UAAUAAU@LB@3'-UUU...CAGUU UA AAAAA ACGGC...ACA-5'@LB@ \| \|@LB@ 51 24@LB@@LB@@LB@Energy -9.18 kcal/mol@LB@Hybridization Energy -15.1619 kcal/mol@LB@Unfolding Energy - mRNA 4.88468 kcal/mol@LB@Unfolding Energy - sRNA 1.09239 kcal/mol@LB@@LB@Position - mRNA 187 -- 212@LB@Position - sRNA 25 -- 50@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 25 -- 31@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching