Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
126	0.00562	0.157	b3017	ftsP	-15.30	0.017476	190 -- 242	38 -- 84	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)	_____________189____________________________________________________243\n_____________\|______________________________________________________\|\n5'-GAC...UUUAC____A_____U__GU___U____CGG____CAG________GGCAUC_______GCACU...CGC-3'\n______________AUGG_GCAAA_AU__CAC_CAGU____CGU___UUCAUUCA______GGGGAUU\n______________\|\|\|\|_\|\|\|\|\|_\|:__\|\|\|_\|\|\|:____\|\|\|___\|\|\|\|:\|\|\|______\|\|\|\|\|\|\|\n______________UACC_CGUUU_UG__GUG_GUCG____GCA___AAGUGAGU______CCCCUAA\n3'-UUU...CCCUC__________C__AU___U____UUAA___ACA_____________________AGGUA...GCA-5'\n_____________\|______________________________________________________\|\n_____________85_____________________________________________________37\n	236 -- 242	38 -- 44	-40.4092 kcal/mol	11.1124 kcal/mol	13.9994 kcal/mol	189 243@LB@ \| \|@LB@5'-GAC...UUUAC A U GU U CGG CAG GGCAUC GCACU...CGC-3'@LB@ AUGG GCAAA AU CAC CAGU CGU UUCAUUCA GGGGAUU@LB@ \|\|\|\| \|\|\|\|\| \|: \|\|\| \|\|\|: \|\|\| \|\|\|\|:\|\|\| \|\|\|\|\|\|\|@LB@ UACC CGUUU UG GUG GUCG GCA AAGUGAGU CCCCUAA@LB@3'-UUU...CCCUC C AU U UUAA ACA AGGUA...GCA-5'@LB@ \| \|@LB@ 85 37@LB@@LB@@LB@Energy -15.30 kcal/mol@LB@Hybridization Energy -40.4092 kcal/mol@LB@Unfolding Energy - mRNA 11.1124 kcal/mol@LB@Unfolding Energy - sRNA 13.9994 kcal/mol@LB@@LB@Position - mRNA 190 -- 242@LB@Position - sRNA 38 -- 84@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 38 -- 44@LB@
26	0.0002514	0.03403	b4551	yheV	-11.83	0.112444	191 -- 229	42 -- 77	DUF2387 family putative metal-binding protein	_____________190_____________________________________230\n_____________\|_______________________________________\|\n5'-AUC...GAGUA____G__A_G________C__AAAAC________GC___CGCAA...UUG-3'\n______________GAGG_UA_A_AUGGCAAU_CG_____GUUUUAUU__GGG\n______________:\|\|:_\|\|_\|_\|::\|\|\|\|\|_\|\|_____\|\|\|\|:\|:\|__\|\|\|\n______________UUCU_AU_U_UGUCGUUA_GC_____CAAAGUGA__CCC\n3'-UUU...CCCGU____G__G_G________A__AA___________GU___CUAAA...GCA-5'\n_____________\|_______________________________________\|\n_____________78______________________________________41\n	217 -- 223	48 -- 54	-24.6454 kcal/mol	8.05545 kcal/mol	4.7648 kcal/mol	190 230@LB@ \| \|@LB@5'-AUC...GAGUA G A G C AAAAC GC CGCAA...UUG-3'@LB@ GAGG UA A AUGGCAAU CG GUUUUAUU GGG@LB@ :\|\|: \|\| \| \|::\|\|\|\|\| \|\| \|\|\|\|:\|:\| \|\|\|@LB@ UUCU AU U UGUCGUUA GC CAAAGUGA CCC@LB@3'-UUU...CCCGU G G G A AA GU CUAAA...GCA-5'@LB@ \| \|@LB@ 78 41@LB@@LB@@LB@Energy -11.83 kcal/mol@LB@Hybridization Energy -24.6454 kcal/mol@LB@Unfolding Energy - mRNA 8.05545 kcal/mol@LB@Unfolding Energy - sRNA 4.7648 kcal/mol@LB@@LB@Position - mRNA 191 -- 229@LB@Position - sRNA 42 -- 77@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 48 -- 54@LB@
180	0.009034	0.1747	b3146	rsmI	-12.76	0.071583	191 -- 238	34 -- 76	16S rRNA C1402 2'-O-ribose methyltransferase SAM-dependent	_____________190____________________________________________________239\n_____________\|______________________________________________________\|\n5'-CAG...AAUAC____G____AAUGAAACA___C_______GC_GAUAAUU_______________GCUUU...AGG-3'\n______________GGAC_ACAC_________ACA_CAA_UCG__G_______CUCAGGG_____CCA\n______________:\|\|\|_\|\|\|\|_________\|\|\|_\|\|\|_\|\|\|__\|_______\|\|\|\|\|\|\|_____\|\|\|\n______________UCUG_UGUG_________UGU_GUU_AGC__C_______GAGUCCC_____GGU\n3'-UUU...CCGUU____A________________C___A___AA_AAAGU_________CUAAA___ACGAA...GCA-5'\n_____________\|______________________________________________________\|\n_____________77_____________________________________________________33\n	229 -- 235	42 -- 48	-25.3144 kcal/mol	7.73901 kcal/mol	4.816 kcal/mol	190 239@LB@ \| \|@LB@5'-CAG...AAUAC G AAUGAAACA C GC GAUAAUU GCUUU...AGG-3'@LB@ GGAC ACAC ACA CAA UCG G CUCAGGG CCA@LB@ :\|\|\| \|\|\|\| \|\|\| \|\|\| \|\|\| \| \|\|\|\|\|\|\| \|\|\|@LB@ UCUG UGUG UGU GUU AGC C GAGUCCC GGU@LB@3'-UUU...CCGUU A C A AA AAAGU CUAAA ACGAA...GCA-5'@LB@ \| \|@LB@ 77 33@LB@@LB@@LB@Energy -12.76 kcal/mol@LB@Hybridization Energy -25.3144 kcal/mol@LB@Unfolding Energy - mRNA 7.73901 kcal/mol@LB@Unfolding Energy - sRNA 4.816 kcal/mol@LB@@LB@Position - mRNA 191 -- 238@LB@Position - sRNA 34 -- 76@LB@Position Seed - mRNA 229 -- 235@LB@Position Seed - sRNA 42 -- 48@LB@
14	0.0001025	0.0245	b4051	qorA	-14.73	0.024599	192 -- 224	48 -- 76	quinone oxidoreductase NADPH-dependent	_____________191_______________________________225\n_____________\|_________________________________\|\n5'-CGC...GUUCU___GG___________CA__AA___AA______AAGCA...UCG-3'\n______________GGA__ACACAUGGCAA__CG__UUG__UUUCAC\n______________:\|\|__\|\|\|\|\|::\|\|\|\|__\|\|__\|\|\|__\|\|\|\|\|\|\n______________UCU__UGUGUGUCGUU__GC__AAC__AAAGUG\n3'-UUU...CCGUU___GA___________AA_______________AGUCC...GCA-5'\n_____________\|_________________________________\|\n_____________77________________________________47\n	201 -- 207	61 -- 67	-23.6874 kcal/mol	5.27405 kcal/mol	3.68714 kcal/mol	191 225@LB@ \| \|@LB@5'-CGC...GUUCU GG CA AA AA AAGCA...UCG-3'@LB@ GGA ACACAUGGCAA CG UUG UUUCAC@LB@ :\|\| \|\|\|\|\|::\|\|\|\| \|\| \|\|\| \|\|\|\|\|\|@LB@ UCU UGUGUGUCGUU GC AAC AAAGUG@LB@3'-UUU...CCGUU GA AA AGUCC...GCA-5'@LB@ \| \|@LB@ 77 47@LB@@LB@@LB@Energy -14.73 kcal/mol@LB@Hybridization Energy -23.6874 kcal/mol@LB@Unfolding Energy - mRNA 5.27405 kcal/mol@LB@Unfolding Energy - sRNA 3.68714 kcal/mol@LB@@LB@Position - mRNA 192 -- 224@LB@Position - sRNA 48 -- 76@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 61 -- 67@LB@
21	0.0001827	0.02926	b1937	fliE	-13.91	0.039102	192 -- 221	38 -- 77	flagellar basal-body component	_____________191________________________________________222\n_____________\|__________________________________________\|\n5'-CUG...UCCGG_____GAGA___U_______A_____________________GAAGG...GUU-3'\n______________GAGAC____AUG_CAGCGAU_____________CAGGGGAUU\n______________:\|\|\|\|____\|::_\|\|\|\|:\|\|_____________\|\|\|\|\|\|\|\|\|\n______________UUCUG____UGU_GUCGUUA_____________GUCCCCUAA\n3'-UUU...CCCGU_____AUG____________AGCAACAAAGUGA_________AGGUA...GCA-5'\n_____________\|__________________________________________\|\n_____________78_________________________________________37\n	215 -- 221	38 -- 44	-25.7557 kcal/mol	7.06678 kcal/mol	4.77969 kcal/mol	191 222@LB@ \| \|@LB@5'-CUG...UCCGG GAGA U A GAAGG...GUU-3'@LB@ GAGAC AUG CAGCGAU CAGGGGAUU@LB@ :\|\|\|\| \|:: \|\|\|\|:\|\| \|\|\|\|\|\|\|\|\|@LB@ UUCUG UGU GUCGUUA GUCCCCUAA@LB@3'-UUU...CCCGU AUG AGCAACAAAGUGA AGGUA...GCA-5'@LB@ \| \|@LB@ 78 37@LB@@LB@@LB@Energy -13.91 kcal/mol@LB@Hybridization Energy -25.7557 kcal/mol@LB@Unfolding Energy - mRNA 7.06678 kcal/mol@LB@Unfolding Energy - sRNA 4.77969 kcal/mol@LB@@LB@Position - mRNA 192 -- 221@LB@Position - sRNA 38 -- 77@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 38 -- 44@LB@
142	0.006549	0.1605	b3902	rhaD	-9.93	0.251242	192 -- 218	44 -- 69	rhamnulose-1-phosphate aldolase	_____________191_________________________219\n_____________\|___________________________\|\n5'-AGA...CAGGA______AACAUGCAAAACA________UCCUG...UGA-3'\n______________ACACAG_____________UUACUCAG\n______________\|\|\|\|\|\|_____________\|:\|\|\|\|\|\|\n______________UGUGUC_____________AGUGAGUC\n3'-UUU...UGAUG______GUUAAGCAACAA_________CCCUA...GCA-5'\n_____________\|___________________________\|\n_____________70__________________________43\n	212 -- 218	44 -- 50	-16.231 kcal/mol	2.20309 kcal/mol	4.09554 kcal/mol	191 219@LB@ \| \|@LB@5'-AGA...CAGGA AACAUGCAAAACA UCCUG...UGA-3'@LB@ ACACAG UUACUCAG@LB@ \|\|\|\|\|\| \|:\|\|\|\|\|\|@LB@ UGUGUC AGUGAGUC@LB@3'-UUU...UGAUG GUUAAGCAACAA CCCUA...GCA-5'@LB@ \| \|@LB@ 70 43@LB@@LB@@LB@Energy -9.93 kcal/mol@LB@Hybridization Energy -16.231 kcal/mol@LB@Unfolding Energy - mRNA 2.20309 kcal/mol@LB@Unfolding Energy - sRNA 4.09554 kcal/mol@LB@@LB@Position - mRNA 192 -- 218@LB@Position - sRNA 44 -- 69@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 44 -- 50@LB@
56	0.001982	0.1223	b2304	yfcH	-16.49	0.008143	193 -- 225	40 -- 77	putative NAD-dependent nucleotide-sugar epimerase	_____________192_________________________________________226\n_____________\|___________________________________________\|\n5'-AAA...AUCGG________AUGA__________________________A____CAGGA...ACC-3'\n______________GAGGCUGC____ACAUAGUGA_________UCAC_CGG_GGGA\n______________:\|\|:\|\|:\|____\|\|\|:\|\|::\|_________\|\|\|\|_\|:\|_\|\|\|\|\n______________UUCUGAUG____UGUGUCGUU_________AGUG_GUC_CCCU\n3'-UUU...CCCGU_____________________AAGCAACAA____A________AAAGG...GCA-5'\n_____________\|___________________________________________\|\n_____________78__________________________________________39\n	206 -- 212	62 -- 68	-29.217 kcal/mol	7.9505 kcal/mol	4.77871 kcal/mol	192 226@LB@ \| \|@LB@5'-AAA...AUCGG AUGA A CAGGA...ACC-3'@LB@ GAGGCUGC ACAUAGUGA UCAC CGG GGGA@LB@ :\|\|:\|\|:\| \|\|\|:\|\|::\| \|\|\|\| \|:\| \|\|\|\|@LB@ UUCUGAUG UGUGUCGUU AGUG GUC CCCU@LB@3'-UUU...CCCGU AAGCAACAA A AAAGG...GCA-5'@LB@ \| \|@LB@ 78 39@LB@@LB@@LB@Energy -16.49 kcal/mol@LB@Hybridization Energy -29.217 kcal/mol@LB@Unfolding Energy - mRNA 7.9505 kcal/mol@LB@Unfolding Energy - sRNA 4.77871 kcal/mol@LB@@LB@Position - mRNA 193 -- 225@LB@Position - sRNA 40 -- 77@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 62 -- 68@LB@
115	0.005088	0.155	b4131	cadA	-16.57	0.007707	197 -- 237	37 -- 75	lysine decarboxylase acid-inducible	_____________196__________________________________________238\n_____________\|____________________________________________\|\n5'-CUC...GAUAU______GA___UUAUU_____A_____AA____AU_________AUUUU...UUG-3'\n______________GACUAU__ACG_____GCAAU___UUG__UCAC___GGGGGUUU\n______________\|\|\|\|\|:__\|\|:_____\|\|\|\|\|___\|\|\|__\|\|\|\|___\|\|\|\|:\|\|\|\n______________CUGAUG__UGU_____CGUUA___AAC__AGUG___CCCCUAAA\n3'-UUU...CGUUU______UG_____________AGC___AA____AGU________GGUAC...GCA-5'\n_____________\|____________________________________________\|\n_____________76___________________________________________36\n	231 -- 237	37 -- 43	-27.1465 kcal/mol	5.79755 kcal/mol	4.77966 kcal/mol	196 238@LB@ \| \|@LB@5'-CUC...GAUAU GA UUAUU A AA AU AUUUU...UUG-3'@LB@ GACUAU ACG GCAAU UUG UCAC GGGGGUUU@LB@ \|\|\|\|\|: \|\|: \|\|\|\|\| \|\|\| \|\|\|\| \|\|\|\|:\|\|\|@LB@ CUGAUG UGU CGUUA AAC AGUG CCCCUAAA@LB@3'-UUU...CGUUU UG AGC AA AGU GGUAC...GCA-5'@LB@ \| \|@LB@ 76 36@LB@@LB@@LB@Energy -16.57 kcal/mol@LB@Hybridization Energy -27.1465 kcal/mol@LB@Unfolding Energy - mRNA 5.79755 kcal/mol@LB@Unfolding Energy - sRNA 4.77966 kcal/mol@LB@@LB@Position - mRNA 197 -- 237@LB@Position - sRNA 37 -- 75@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 37 -- 43@LB@
34	0.0005364	0.05552	b4244	pyrI	-10.55	0.196904	198 -- 215	60 -- 77	aspartate carbamoyltransferase regulatory subunit	_____________197________________216\n_____________\|__________________\|\n5'-UUC...AAAUA_____G_______A____AAAUU...UCA-3'\n______________GAGAU_ACACACG_UAAU\n______________:\|\|\|:_\|\|\|\|\|\|:_:\|\|\|\n______________UUCUG_UGUGUGU_GUUA\n3'-UUU...CCCGU_____A_______C____AGCAA...GCA-5'\n_____________\|__________________\|\n_____________78_________________59\n	204 -- 210	65 -- 71	-15.1619 kcal/mol	1.32522 kcal/mol	3.29036 kcal/mol	197 216@LB@ \| \|@LB@5'-UUC...AAAUA G A AAAUU...UCA-3'@LB@ GAGAU ACACACG UAAU@LB@ :\|\|\|: \|\|\|\|\|\|: :\|\|\|@LB@ UUCUG UGUGUGU GUUA@LB@3'-UUU...CCCGU A C AGCAA...GCA-5'@LB@ \| \|@LB@ 78 59@LB@@LB@@LB@Energy -10.55 kcal/mol@LB@Hybridization Energy -15.1619 kcal/mol@LB@Unfolding Energy - mRNA 1.32522 kcal/mol@LB@Unfolding Energy - sRNA 3.29036 kcal/mol@LB@@LB@Position - mRNA 198 -- 215@LB@Position - sRNA 60 -- 77@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 65 -- 71@LB@
53	0.001859	0.1218	b0529	folD	-11.41	0.136116	200 -- 226	61 -- 85	bifunctional 5 10-methylene-tetrahydrofolate dehydrogenase/ 5 10-methylene-tetrahydrofolate cyclohydrolase	_____________199_________________________227\n_____________\|___________________________\|\n5'-CAA...UCUCU_____CA___________UG_______AACGA...CAC-3'\n______________GAUGG__GCAAAGAUUAU__ACGGUAA\n______________\|\|\|\|\|__\|\|\|\|\|\|\|:\|\|:__\|\|:\|:\|\|\n______________CUACC__CGUUUCUGAUG__UGUCGUU\n3'-UUU...ACCCU__________________UG_______AAGCA...GCA-5'\n_____________\|___________________________\|\n_____________86__________________________60\n	220 -- 226	61 -- 67	-27.4215 kcal/mol	5.77154 kcal/mol	10.2439 kcal/mol	199 227@LB@ \| \|@LB@5'-CAA...UCUCU CA UG AACGA...CAC-3'@LB@ GAUGG GCAAAGAUUAU ACGGUAA@LB@ \|\|\|\|\| \|\|\|\|\|\|\|:\|\|: \|\|:\|:\|\|@LB@ CUACC CGUUUCUGAUG UGUCGUU@LB@3'-UUU...ACCCU UG AAGCA...GCA-5'@LB@ \| \|@LB@ 86 60@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -27.4215 kcal/mol@LB@Unfolding Energy - mRNA 5.77154 kcal/mol@LB@Unfolding Energy - sRNA 10.2439 kcal/mol@LB@@LB@Position - mRNA 200 -- 226@LB@Position - sRNA 61 -- 85@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 61 -- 67@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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