| 171 |
0.01646 |
0.3152 |
bsu04650 |
endB |
-11.87 |
0.110785 |
167 -- 193 |
29 -- 53 |
antitoxin EndoAI |
|
_____________166_________________________194\n_____________|___________________________|\n5'-UUC...AAGAG___U___CGUAA____________U__AUGUU...GAA-3'\n______________UCG_AUC_____GUUUGGUGGAGG_GU\n______________|||_|||_____:|:||:|:||||_||\n______________AGC_UAG_____UAGACUAUCUCC_CA\n3'-UUU...AAAAA___C___AAC______________C__ACCCC...AUA-5'\n_____________|___________________________|\n_____________54__________________________28\n |
184 -- 190 |
32 -- 38 |
-19.891 kcal/mol |
7.03825 kcal/mol |
0.985603 kcal/mol |
166 194@LB@ | |@LB@5'-UUC...AAGAG U CGUAA U AUGUU...GAA-3'@LB@ UCG AUC GUUUGGUGGAGG GU@LB@ ||| ||| :|:||:|:|||| ||@LB@ AGC UAG UAGACUAUCUCC CA@LB@3'-UUU...AAAAA C AAC C ACCCC...AUA-5'@LB@ | |@LB@ 54 28@LB@@LB@@LB@Energy -11.87 kcal/mol@LB@Hybridization Energy -19.891 kcal/mol@LB@Unfolding Energy - mRNA 7.03825 kcal/mol@LB@Unfolding Energy - sRNA 0.985603 kcal/mol@LB@@LB@Position - mRNA 167 -- 193@LB@Position - sRNA 29 -- 53@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 198 |
0.0197 |
0.3269 |
bsu08980 |
yhbH |
-11.85 |
0.111658 |
189 -- 195 |
30 -- 36 |
stress response UPF0229 protein YhbH |
|
_____________188_____196\n_____________|_______|\n5'-GGC...GUUAA_______AAUUC...UGC-3'\n______________GGAGGGG\n______________:||||||\n______________UCUCCCC\n3'-UUU...GACUA_______AACCC...AUA-5'\n_____________|_______|\n_____________37______29\n |
189 -- 195 |
30 -- 36 |
-13.8096 kcal/mol |
1.82966 kcal/mol |
0.133643 kcal/mol |
188 196@LB@ | |@LB@5'-GGC...GUUAA AAUUC...UGC-3'@LB@ GGAGGGG@LB@ :||||||@LB@ UCUCCCC@LB@3'-UUU...GACUA AACCC...AUA-5'@LB@ | |@LB@ 37 29@LB@@LB@@LB@Energy -11.85 kcal/mol@LB@Hybridization Energy -13.8096 kcal/mol@LB@Unfolding Energy - mRNA 1.82966 kcal/mol@LB@Unfolding Energy - sRNA 0.133643 kcal/mol@LB@@LB@Position - mRNA 189 -- 195@LB@Position - sRNA 30 -- 36@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 74 |
0.005527 |
0.2441 |
bsu14880 |
ctaB |
-11.75 |
0.115909 |
1 -- 10 |
34 -- 43 |
protoheme IX farnesyltransferase 2 |
|
______________1_________11\n______________|_________|\n___________5'-__________AAUAA...UCG-3'\n______________GUCUGAUAGA\n______________:|||||||||\n______________UAGACUAUCU\n3'-UUU...AGAAC__________CCCCA...AUA-5'\n_____________|__________|\n_____________44_________33\n |
4 -- 10 |
34 -- 40 |
-13.2371 kcal/mol |
0.533082 kcal/mol |
0.957751 kcal/mol |
1 11@LB@ | |@LB@ 5'- AAUAA...UCG-3'@LB@ GUCUGAUAGA@LB@ :|||||||||@LB@ UAGACUAUCU@LB@3'-UUU...AGAAC CCCCA...AUA-5'@LB@ | |@LB@ 44 33@LB@@LB@@LB@Energy -11.75 kcal/mol@LB@Hybridization Energy -13.2371 kcal/mol@LB@Unfolding Energy - mRNA 0.533082 kcal/mol@LB@Unfolding Energy - sRNA 0.957751 kcal/mol@LB@@LB@Position - mRNA 1 -- 10@LB@Position - sRNA 34 -- 43@LB@Position Seed - mRNA 4 -- 10@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 114 |
0.009837 |
0.2871 |
bsu30580 |
ytmA |
-11.73 |
0.116519 |
193 -- 212 |
33 -- 52 |
peptidase YtmA |
|
_____________192____________________213\n_____________|______________________|\n5'-AGA...GGAGG___CU_________________AAAAG...UGG-3'\n______________CGG__GUUUUGAUU__GUAGAG\n______________|||__:|:|||||:__:|||||\n______________GCC__UAGAACUAG__UAUCUC\n3'-UUU...AAAAA______________AC______CCCAA...AUA-5'\n_____________|______________________|\n_____________53_____________________32\n |
200 -- 206 |
41 -- 47 |
-17.5653 kcal/mol |
4.85678 kcal/mol |
0.976367 kcal/mol |
192 213@LB@ | |@LB@5'-AGA...GGAGG CU AAAAG...UGG-3'@LB@ CGG GUUUUGAUU GUAGAG@LB@ ||| :|:|||||: :|||||@LB@ GCC UAGAACUAG UAUCUC@LB@3'-UUU...AAAAA AC CCCAA...AUA-5'@LB@ | |@LB@ 53 32@LB@@LB@@LB@Energy -11.73 kcal/mol@LB@Hybridization Energy -17.5653 kcal/mol@LB@Unfolding Energy - mRNA 4.85678 kcal/mol@LB@Unfolding Energy - sRNA 0.976367 kcal/mol@LB@@LB@Position - mRNA 193 -- 212@LB@Position - sRNA 33 -- 52@LB@Position Seed - mRNA 200 -- 206@LB@Position Seed - sRNA 41 -- 47@LB@ |
| 80 |
0.005932 |
0.2448 |
bsu24430 |
spoIIIAA |
-11.72 |
0.117179 |
88 -- 116 |
28 -- 52 |
stage III sporulation protein AA |
|
_____________87______________________________117\n_____________|_______________________________|\n5'-GUC...UUUUA___GUGUU_______________C___U___CAUAA...GGA-3'\n______________CGG_____GUCUUGAUUU__UGG_GGG_GUU\n______________|||_____:|||||||:|__|:|_|||_|||\n______________GCC_____UAGAACUAGA__AUC_CCC_CAA\n3'-UUU...AAAAA__________________CU___U_______CCCCU...AUA-5'\n_____________|_______________________________|\n_____________53______________________________27\n |
99 -- 105 |
40 -- 46 |
-20.9874 kcal/mol |
8.2899 kcal/mol |
0.980555 kcal/mol |
87 117@LB@ | |@LB@5'-GUC...UUUUA GUGUU C U CAUAA...GGA-3'@LB@ CGG GUCUUGAUUU UGG GGG GUU@LB@ ||| :|||||||:| |:| ||| |||@LB@ GCC UAGAACUAGA AUC CCC CAA@LB@3'-UUU...AAAAA CU U CCCCU...AUA-5'@LB@ | |@LB@ 53 27@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -20.9874 kcal/mol@LB@Unfolding Energy - mRNA 8.2899 kcal/mol@LB@Unfolding Energy - sRNA 0.980555 kcal/mol@LB@@LB@Position - mRNA 88 -- 116@LB@Position - sRNA 28 -- 52@LB@Position Seed - mRNA 99 -- 105@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 122 |
0.01077 |
0.2883 |
bsu18910 |
rapK |
-11.72 |
0.116857 |
189 -- 197 |
28 -- 36 |
response regulator aspartate phosphatase K |
|
_____________188_______198\n_____________|_________|\n5'-UAU...CAGAA_________UAAAU...CAG-3'\n______________AGAGGGGUU\n______________|||||||||\n______________UCUCCCCAA\n3'-UUU...GACUA_________CCCCU...AUA-5'\n_____________|_________|\n_____________37________27\n |
191 -- 197 |
28 -- 34 |
-15.633 kcal/mol |
3.77416 kcal/mol |
0.134381 kcal/mol |
188 198@LB@ | |@LB@5'-UAU...CAGAA UAAAU...CAG-3'@LB@ AGAGGGGUU@LB@ |||||||||@LB@ UCUCCCCAA@LB@3'-UUU...GACUA CCCCU...AUA-5'@LB@ | |@LB@ 37 27@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -15.633 kcal/mol@LB@Unfolding Energy - mRNA 3.77416 kcal/mol@LB@Unfolding Energy - sRNA 0.134381 kcal/mol@LB@@LB@Position - mRNA 189 -- 197@LB@Position - sRNA 28 -- 36@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 160 |
0.01451 |
0.3008 |
bsu30080 |
yteV |
-11.71 |
0.117611 |
279 -- 298 |
34 -- 53 |
sporulation protein cse60 |
|
_____________278__________________299\n_____________|____________________|\n5'-UUU...GGAUA_____GGAGCAG________UA-3'\n______________UCGGA_______CUGAUAGA\n______________|||||_______||||||||\n______________AGCCU_______GACUAUCU\n3'-UUU...AAAAA_____AGAACUA________CCCCA...AUA-5'\n_____________|____________________|\n_____________54___________________33\n |
292 -- 298 |
34 -- 40 |
-18.1644 kcal/mol |
5.47565 kcal/mol |
0.98171 kcal/mol |
278 299@LB@ | |@LB@5'-UUU...GGAUA GGAGCAG UA-3'@LB@ UCGGA CUGAUAGA@LB@ ||||| ||||||||@LB@ AGCCU GACUAUCU@LB@3'-UUU...AAAAA AGAACUA CCCCA...AUA-5'@LB@ | |@LB@ 54 33@LB@@LB@@LB@Energy -11.71 kcal/mol@LB@Hybridization Energy -18.1644 kcal/mol@LB@Unfolding Energy - mRNA 5.47565 kcal/mol@LB@Unfolding Energy - sRNA 0.98171 kcal/mol@LB@@LB@Position - mRNA 279 -- 298@LB@Position - sRNA 34 -- 53@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 192 |
0.01921 |
0.3269 |
bsu12649 |
ykzM |
-11.69 |
0.118448 |
82 -- 103 |
11 -- 32 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu26089 |
_____________81______________________104\n_____________|_______________________|\n5'-AAG...GACAA____A____________C_A___GGCCA...UCG-3'\n______________GGGU__GGGGACAGGGG_G_GGC\n______________||||__|||:||||:|:_|_:||\n______________CCCA__CCCUUGUCUCU_C_UCG\n3'-UUU...AUCUC____AC_____________A___AAGAG...AUA-5'\n_____________|_______________________|\n_____________33______________________10\n |
88 -- 94 |
19 -- 25 |
-26.088 kcal/mol |
7.89329 kcal/mol |
6.50677 kcal/mol |
81 104@LB@ | |@LB@5'-AAG...GACAA A C A GGCCA...UCG-3'@LB@ GGGU GGGGACAGGGG G GGC@LB@ |||| |||:||||:|: | :||@LB@ CCCA CCCUUGUCUCU C UCG@LB@3'-UUU...AUCUC AC A AAGAG...AUA-5'@LB@ | |@LB@ 33 10@LB@@LB@@LB@Energy -11.69 kcal/mol@LB@Hybridization Energy -26.088 kcal/mol@LB@Unfolding Energy - mRNA 7.89329 kcal/mol@LB@Unfolding Energy - sRNA 6.50677 kcal/mol@LB@@LB@Position - mRNA 82 -- 103@LB@Position - sRNA 11 -- 32@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 79 |
0.005838 |
0.2448 |
bsu25980 |
yqbT |
-11.66 |
0.119794 |
52 -- 65 |
32 -- 53 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type ps: structure' |
bsu12730 |
_____________51_____________________66\n_____________|______________________|\n5'-CGU...AUCCC______________________AAGCA...CGU-3'\n______________UCGG________CUGAUAGAGG\n______________||||________||||||||||\n______________AGCC________GACUAUCUCC\n3'-UUU...AAAAA____UAGAACUA__________CCAAC...AUA-5'\n_____________|______________________|\n_____________54_____________________31\n |
59 -- 65 |
32 -- 38 |
-19.4839 kcal/mol |
6.84464 kcal/mol |
0.981755 kcal/mol |
51 66@LB@ | |@LB@5'-CGU...AUCCC AAGCA...CGU-3'@LB@ UCGG CUGAUAGAGG@LB@ |||| ||||||||||@LB@ AGCC GACUAUCUCC@LB@3'-UUU...AAAAA UAGAACUA CCAAC...AUA-5'@LB@ | |@LB@ 54 31@LB@@LB@@LB@Energy -11.66 kcal/mol@LB@Hybridization Energy -19.4839 kcal/mol@LB@Unfolding Energy - mRNA 6.84464 kcal/mol@LB@Unfolding Energy - sRNA 0.981755 kcal/mol@LB@@LB@Position - mRNA 52 -- 65@LB@Position - sRNA 32 -- 53@LB@Position Seed - mRNA 59 -- 65@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 175 |
0.01672 |
0.3152 |
bsu04630 |
ydcC |
-11.64 |
0.120416 |
196 -- 203 |
27 -- 34 |
sporulation protein YdcC |
|
_____________195______204\n_____________|________|\n5'-CCG...GAAAA________AAAAA...UGG-3'\n______________AGGGGUUG\n______________||||||||\n______________UCCCCAAC\n3'-UUU...CUAUC________CCCUU...AUA-5'\n_____________|________|\n_____________35_______26\n |
197 -- 203 |
27 -- 33 |
-14.5305 kcal/mol |
2.76844 kcal/mol |
0.118612 kcal/mol |
195 204@LB@ | |@LB@5'-CCG...GAAAA AAAAA...UGG-3'@LB@ AGGGGUUG@LB@ ||||||||@LB@ UCCCCAAC@LB@3'-UUU...CUAUC CCCUU...AUA-5'@LB@ | |@LB@ 35 26@LB@@LB@@LB@Energy -11.64 kcal/mol@LB@Hybridization Energy -14.5305 kcal/mol@LB@Unfolding Energy - mRNA 2.76844 kcal/mol@LB@Unfolding Energy - sRNA 0.118612 kcal/mol@LB@@LB@Position - mRNA 196 -- 203@LB@Position - sRNA 27 -- 34@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 27 -- 33@LB@ |
