Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
47	0.002271	0.1648	b3169	nusA	-13.61	0.058009	55 -- 79	4 -- 28	transcription termination/antitermination L factor		_____________54__________________________80\n_____________\|___________________________\|\n5'-UUG...UCGUA_____CA________UC__________AGACG...CAG-3'\n______________AAUGG__GGGCGUUA____AAAGCGGU\n______________\|\|\|\|\|__\|\|:\|:\|:\|____\|\|\|\|\|:\|\|\n______________UUACC__CCUGUAGU____UUUCGUCA\n3'-UUU...GUGUU_______________UUCU________CCG-5'\n_____________\|___________________________\|\n_____________29__________________________3\n	73 -- 79	4 -- 10	-21.8237 kcal/mol	5.69952 kcal/mol	2.51457 kcal/mol	54 80@LB@ \| \|@LB@5'-UUG...UCGUA CA UC AGACG...CAG-3'@LB@ AAUGG GGGCGUUA AAAGCGGU@LB@ \|\|\|\|\| \|\|:\|:\|:\| \|\|\|\|\|:\|\|@LB@ UUACC CCUGUAGU UUUCGUCA@LB@3'-UUU...GUGUU UUCU CCG-5'@LB@ \| \|@LB@ 29 3@LB@@LB@@LB@Energy -13.61 kcal/mol@LB@Hybridization Energy -21.8237 kcal/mol@LB@Unfolding Energy - mRNA 5.69952 kcal/mol@LB@Unfolding Energy - sRNA 2.51457 kcal/mol@LB@@LB@Position - mRNA 55 -- 79@LB@Position - sRNA 4 -- 28@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 4 -- 10@LB@
180	0.01303	0.2504	b3487	yhiI	-13.61	0.058128	230 -- 256	1 -- 27	putative membrane fusion protein (MFP) of efflux pump		_____________229__________________________257\n_____________\|____________________________\|\n5'-UUU...GCGUG_____U________UUACU_________GGCUA...CGG-3'\n______________GUGGG__UGUCGGG_____GGCGGUGGC\n______________:\|\|\|\|__::\|\|:::_____:\|\|:\|\|\|\|\|\n______________UACCC__GUAGUUU_____UCGUCACCG\n3'-UUU...UGUUU_____CU_______CUUU__________-5'\n_____________\|___________________________\|\n_____________28__________________________1\n	250 -- 256	1 -- 7	-27.1902 kcal/mol	11.0661 kcal/mol	2.51867 kcal/mol	229 257@LB@ \| \|@LB@5'-UUU...GCGUG U UUACU GGCUA...CGG-3'@LB@ GUGGG UGUCGGG GGCGGUGGC@LB@ :\|\|\|\| ::\|\|::: :\|\|:\|\|\|\|\|@LB@ UACCC GUAGUUU UCGUCACCG@LB@3'-UUU...UGUUU CU CUUU -5'@LB@ \| \|@LB@ 28 1@LB@@LB@@LB@Energy -13.61 kcal/mol@LB@Hybridization Energy -27.1902 kcal/mol@LB@Unfolding Energy - mRNA 11.0661 kcal/mol@LB@Unfolding Energy - sRNA 2.51867 kcal/mol@LB@@LB@Position - mRNA 230 -- 256@LB@Position - sRNA 1 -- 27@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 1 -- 7@LB@
38	0.001836	0.1648	b3188	sfsB	-13.60	0.058336	253 -- 274	1 -- 32	malPQ operon transcriptional activator		_____________252______________________________275\n_____________\|________________________________\|\n5'-CGG...UUUGC______A___A_____________________GGCGG...UGA-3'\n______________GCAAGA_GGG_ACGUCAA__________UGGC\n______________:\|\|\|:\|_\|\|\|_\|\|:\|\|\|\|__________\|\|\|\|\n______________UGUUUU_CCC_UGUAGUU__________ACCG\n3'-UUU...CGAGG______A___C_______UCUUUUCGUC____-5'\n_____________\|_______________________________\|\n_____________33______________________________1\n	264 -- 270	15 -- 21	-21.5209 kcal/mol	5.38314 kcal/mol	2.53929 kcal/mol	252 275@LB@ \| \|@LB@5'-CGG...UUUGC A A GGCGG...UGA-3'@LB@ GCAAGA GGG ACGUCAA UGGC@LB@ :\|\|\|:\| \|\|\| \|\|:\|\|\|\| \|\|\|\|@LB@ UGUUUU CCC UGUAGUU ACCG@LB@3'-UUU...CGAGG A C UCUUUUCGUC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -13.60 kcal/mol@LB@Hybridization Energy -21.5209 kcal/mol@LB@Unfolding Energy - mRNA 5.38314 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 253 -- 274@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 15 -- 21@LB@
176	0.01279	0.2504	b3980	tufB	-13.59	0.058494	200 -- 216	7 -- 22	translation elongation factor EF-Tu 2	b3339	_____________199_______________217\n_____________\|_________________\|\n5'-UCC...CAAUC______U__________UUGAA...CUA-3'\n______________GAUGUC_AAAGAAAAGU\n______________\|\|::\|\|_\|\|\|\|\|\|\|\|\|:\n______________CUGUAG_UUUCUUUUCG\n3'-UUU...UACCC_________________UCACCG-5'\n_____________\|_________________\|\n_____________23________________6\n	210 -- 216	7 -- 13	-16.0105 kcal/mol	1.82662 kcal/mol	0.590875 kcal/mol	199 217@LB@ \| \|@LB@5'-UCC...CAAUC U UUGAA...CUA-3'@LB@ GAUGUC AAAGAAAAGU@LB@ \|\|::\|\| \|\|\|\|\|\|\|\|\|:@LB@ CUGUAG UUUCUUUUCG@LB@3'-UUU...UACCC UCACCG-5'@LB@ \| \|@LB@ 23 6@LB@@LB@@LB@Energy -13.59 kcal/mol@LB@Hybridization Energy -16.0105 kcal/mol@LB@Unfolding Energy - mRNA 1.82662 kcal/mol@LB@Unfolding Energy - sRNA 0.590875 kcal/mol@LB@@LB@Position - mRNA 200 -- 216@LB@Position - sRNA 7 -- 22@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 7 -- 13@LB@
160	0.0118	0.2504	b0078	ilvH	-13.58	0.059018	151 -- 175	1 -- 25	acetolactate synthase 3 small subunit valine-sensitive		_____________150_______________________176\n_____________\|_________________________\|\n5'-GCC...UUCGC_______GAAU___UGAAAU_____UAAGC...UGA-3'\n______________GGGGGCG____GGA______GUGGU\n______________\|\|\|\|:\|:____:\|\|______\|\|\|\|:\n______________CCCCUGU____UCU______CACCG\n3'-UUU...UUUUA_______AGUU___UUUCGU_____-5'\n_____________\|________________________\|\n_____________26_______________________1\n	151 -- 157	19 -- 25	-21.9405 kcal/mol	5.84786 kcal/mol	2.51748 kcal/mol	150 176@LB@ \| \|@LB@5'-GCC...UUCGC GAAU UGAAAU UAAGC...UGA-3'@LB@ GGGGGCG GGA GUGGU@LB@ \|\|\|\|:\|: :\|\| \|\|\|\|:@LB@ CCCCUGU UCU CACCG@LB@3'-UUU...UUUUA AGUU UUUCGU -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -13.58 kcal/mol@LB@Hybridization Energy -21.9405 kcal/mol@LB@Unfolding Energy - mRNA 5.84786 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 151 -- 175@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 19 -- 25@LB@
96	0.005909	0.216	b0511	ybbW	-13.57	0.059054	201 -- 236	1 -- 27	putative allantoin transporter		_____________200___________________________________237\n_____________\|_____________________________________\|\n5'-UGG...GAUUU____A_____________ACUAUUCC____AC_C___UAUAG...UUA-3'\n______________AUGG__ACAUCAGAGAAA________AGCA__G_GGC\n______________\|\|\|\|__\|\|\|\|\|\|:\|\|\|\|\|________\|\|\|\|__\|_\|\|\|\n______________UACC__UGUAGUUUCUUU________UCGU__C_CCG\n3'-UUU...UGUUU____CC___________________________A___-5'\n_____________\|____________________________________\|\n_____________28___________________________________1\n	211 -- 217	10 -- 16	-23.7564 kcal/mol	7.66376 kcal/mol	2.51867 kcal/mol	200 237@LB@ \| \|@LB@5'-UGG...GAUUU A ACUAUUCC AC C UAUAG...UUA-3'@LB@ AUGG ACAUCAGAGAAA AGCA G GGC@LB@ \|\|\|\| \|\|\|\|\|\|:\|\|\|\|\| \|\|\|\| \| \|\|\|@LB@ UACC UGUAGUUUCUUU UCGU C CCG@LB@3'-UUU...UGUUU CC A -5'@LB@ \| \|@LB@ 28 1@LB@@LB@@LB@Energy -13.57 kcal/mol@LB@Hybridization Energy -23.7564 kcal/mol@LB@Unfolding Energy - mRNA 7.66376 kcal/mol@LB@Unfolding Energy - sRNA 2.51867 kcal/mol@LB@@LB@Position - mRNA 201 -- 236@LB@Position - sRNA 1 -- 27@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 10 -- 16@LB@
72	0.004007	0.1953	b2175	mepS	-13.55	0.059767	177 -- 194	6 -- 23	murein DD-endopeptidase space-maker hydrolase mutational suppressor of prc thermosensitivity outer membrane lipoprotein weak murein LD-carboxypeptidase		_____________176________________195\n_____________\|__________________\|\n5'-CAA...GUUAA____U__________A__AACAA...CAA-3'\n______________GGAC_UCAAGGGAAA_CA\n______________\|\|\|\|_\|\|\|\|:\|:\|\|\|_\|\|\n______________CCUG_AGUUUCUUUU_GU\n3'-UUU...UUACC____U__________C__CACCG-5'\n_____________\|__________________\|\n_____________24_________________5\n	185 -- 191	9 -- 15	-17.1277 kcal/mol	2.97845 kcal/mol	0.599171 kcal/mol	176 195@LB@ \| \|@LB@5'-CAA...GUUAA U A AACAA...CAA-3'@LB@ GGAC UCAAGGGAAA CA@LB@ \|\|\|\| \|\|\|\|:\|:\|\|\| \|\|@LB@ CCUG AGUUUCUUUU GU@LB@3'-UUU...UUACC U C CACCG-5'@LB@ \| \|@LB@ 24 5@LB@@LB@@LB@Energy -13.55 kcal/mol@LB@Hybridization Energy -17.1277 kcal/mol@LB@Unfolding Energy - mRNA 2.97845 kcal/mol@LB@Unfolding Energy - sRNA 0.599171 kcal/mol@LB@@LB@Position - mRNA 177 -- 194@LB@Position - sRNA 6 -- 23@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 9 -- 15@LB@
109	0.007372	0.2348	b3701	dnaN	-13.44	0.063163	149 -- 162	9 -- 22	DNA polymerase III beta subunit		_____________148____________163\n_____________\|______________\|\n5'-GGC...GCCAC______________UUUUU...UGC-3'\n______________GAUAUCAAAGAAGA\n______________\|\|:\|\|\|\|\|\|\|\|\|:\|\n______________CUGUAGUUUCUUUU\n3'-UUU...UACCC______________CGUCACCG-5'\n_____________\|______________\|\n_____________23_____________8\n	156 -- 162	9 -- 15	-16.0152 kcal/mol	2.0875 kcal/mol	0.488786 kcal/mol	148 163@LB@ \| \|@LB@5'-GGC...GCCAC UUUUU...UGC-3'@LB@ GAUAUCAAAGAAGA@LB@ \|\|:\|\|\|\|\|\|\|\|\|:\|@LB@ CUGUAGUUUCUUUU@LB@3'-UUU...UACCC CGUCACCG-5'@LB@ \| \|@LB@ 23 8@LB@@LB@@LB@Energy -13.44 kcal/mol@LB@Hybridization Energy -16.0152 kcal/mol@LB@Unfolding Energy - mRNA 2.0875 kcal/mol@LB@Unfolding Energy - sRNA 0.488786 kcal/mol@LB@@LB@Position - mRNA 149 -- 162@LB@Position - sRNA 9 -- 22@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 9 -- 15@LB@
169	0.01228	0.2504	b3386	rpe	-13.36	0.065831	171 -- 203	3 -- 33	D-ribulose-5-phosphate 3-epimerase		_____________170_______________________________204\n_____________\|_________________________________\|\n5'-CGC...UCACC_______AAAUAAUUC______________A__AAACA...UGC-3'\n______________CGCGAAA_________UCAAGGAGAAGCGG_UG\n______________\|:\|:\|\|\|_________\|\|\|\|:\|\|:\|\|\|\|:\|_\|\|\n______________GUGUUUU_________AGUUUCUUUUCGUC_AC\n3'-UUU...CCGAG_______ACCCCUGU__________________CG-5'\n_____________\|_________________________________\|\n_____________34________________________________2\n	194 -- 200	5 -- 11	-23.2607 kcal/mol	6.82691 kcal/mol	3.0786 kcal/mol	170 204@LB@ \| \|@LB@5'-CGC...UCACC AAAUAAUUC A AAACA...UGC-3'@LB@ CGCGAAA UCAAGGAGAAGCGG UG@LB@ \|:\|:\|\|\| \|\|\|\|:\|\|:\|\|\|\|:\| \|\|@LB@ GUGUUUU AGUUUCUUUUCGUC AC@LB@3'-UUU...CCGAG ACCCCUGU CG-5'@LB@ \| \|@LB@ 34 2@LB@@LB@@LB@Energy -13.36 kcal/mol@LB@Hybridization Energy -23.2607 kcal/mol@LB@Unfolding Energy - mRNA 6.82691 kcal/mol@LB@Unfolding Energy - sRNA 3.0786 kcal/mol@LB@@LB@Position - mRNA 171 -- 203@LB@Position - sRNA 3 -- 33@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 5 -- 11@LB@
94	0.005808	0.2147	b0652	gltL	-13.33	0.066498	266 -- 292	1 -- 31	glutamate/aspartate ABC transporter ATPase		_____________265_____________________________293\n_____________\|_______________________________\|\n5'-CUC...UCAAC_______AAAA________C___________GGUUUGCG-3'\n______________CGAAGUG________AAGG_GAAGUGGUGGU\n______________\|:\|\|:\|\|________\|\|:\|_:\|\|\|::\|\|\|\|:\n______________GUUUUAC________UUUC_UUUCGUCACCG\n3'-UUU...GAGGU_______CCCUGUAG____U___________-5'\n_____________\|______________________________\|\n_____________32_____________________________1\n	286 -- 292	1 -- 7	-20.9442 kcal/mol	5.07032 kcal/mol	2.53929 kcal/mol	265 293@LB@ \| \|@LB@5'-CUC...UCAAC AAAA C GGUUUGCG-3'@LB@ CGAAGUG AAGG GAAGUGGUGGU@LB@ \|:\|\|:\|\| \|\|:\| :\|\|\|::\|\|\|\|:@LB@ GUUUUAC UUUC UUUCGUCACCG@LB@3'-UUU...GAGGU CCCUGUAG U -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -13.33 kcal/mol@LB@Hybridization Energy -20.9442 kcal/mol@LB@Unfolding Energy - mRNA 5.07032 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 266 -- 292@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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