Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
101	0.026	0.6733	lmo2768	N/A	-17.52	0.032492	230 -- 251	161 -- 179	hypothetical membrane protein	_____________229__________252\n_____________\|____________\|\n5'-UUA...GUAAU____________CUUUU...UAA-3'\n______________AUGAGAUGGAUG\n______________\|\|\|\|\|\|\|\|:\|\|\|\n______________UACUCUACUUAC\n3'-GUU...CCGGU____________UUUUG...GGA-5'\n_____________\|____________\|\n_____________180__________160\n	245 -- 251	161 -- 167	-15.292 kcal/mol	2.52317 kcal/mol	0.818518 kcal/mol	229 252@LB@ \| \|@LB@5'-UUA...GUAAU CUUUU...UAA-3'@LB@ AUGAGAUGGAUG@LB@ \|\|\|\|\|\|\|\|:\|\|\|@LB@ UACUCUACUUAC@LB@3'-GUU...CCGGU UUUUG...GGA-5'@LB@ \| \|@LB@ 180 160@LB@@LB@@LB@Energy -17.52 kcal/mol@LB@Hybridization Energy -15.292 kcal/mol@LB@Unfolding Energy - mRNA 2.52317 kcal/mol@LB@Unfolding Energy - sRNA 0.818518 kcal/mol@LB@@LB@Position - mRNA 230 -- 251@LB@Position - sRNA 161 -- 179@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 161 -- 167@LB@
102	0.02619	0.6733	lmo1799	N/A	-17.02	0.040600	38 -- 69	171 -- 207	peptidoglycan binding protein	_____________37______________70\n_____________\|_______________\|\n5'-CUA...AAAAC__G____________UUGGC...UGG-3'\n______________GA_GGGA_GGGGCAU\n______________:\|_\|\|\|\|_\|:\|\|\|\|\|\n______________UU_CCCU_CUCCGUA\n3'-GUU...ACGUA__G____A_______UCUGU...GGA-5'\n_____________\|_______________\|\n_____________208_____________170\n	45 -- 51	193 -- 199	-19.1902 kcal/mol	1.80232 kcal/mol	8.95053 kcal/mol	37 70@LB@ \| \|@LB@5'-CUA...AAAAC G UUGGC...UGG-3'@LB@ GA GGGA GGGGCAU@LB@ :\| \|\|\|\| \|:\|\|\|\|\|@LB@ UU CCCU CUCCGUA@LB@3'-GUU...ACGUA G A UCUGU...GGA-5'@LB@ \| \|@LB@ 208 170@LB@@LB@@LB@Energy -17.02 kcal/mol@LB@Hybridization Energy -19.1902 kcal/mol@LB@Unfolding Energy - mRNA 1.80232 kcal/mol@LB@Unfolding Energy - sRNA 8.95053 kcal/mol@LB@@LB@Position - mRNA 38 -- 69@LB@Position - sRNA 171 -- 207@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 193 -- 199@LB@
103	0.02634	0.6733	lmo2183	N/A	-17.72	0.029644	120 -- 189	95 -- 172	ferrichrome ABC transporter permease	_____________119_______________190\n_____________\|_________________\|\n5'-AGA...UGUGU____UU___________UUAUU...UCC-3'\n______________AUGA__UGGAUGAAAAU\n______________\|\|\|\|__\|\|:\|\|\|\|\|\|\|:\n______________UACU__ACUUACUUUUG\n3'-GUU...CCGGU____CU___________UUUAU...GGA-5'\n_____________\|_________________\|\n_____________173_______________94\n	130 -- 136	156 -- 162	-14.4355 kcal/mol	3.54286 kcal/mol	0.922045 kcal/mol	119 190@LB@ \| \|@LB@5'-AGA...UGUGU UU UUAUU...UCC-3'@LB@ AUGA UGGAUGAAAAU@LB@ \|\|\|\| \|\|:\|\|\|\|\|\|\|:@LB@ UACU ACUUACUUUUG@LB@3'-GUU...CCGGU CU UUUAU...GGA-5'@LB@ \| \|@LB@ 173 94@LB@@LB@@LB@Energy -17.72 kcal/mol@LB@Hybridization Energy -14.4355 kcal/mol@LB@Unfolding Energy - mRNA 3.54286 kcal/mol@LB@Unfolding Energy - sRNA 0.922045 kcal/mol@LB@@LB@Position - mRNA 120 -- 189@LB@Position - sRNA 95 -- 172@LB@Position Seed - mRNA 130 -- 136@LB@Position Seed - sRNA 156 -- 162@LB@
104	0.02699	0.6733	lmo1327	rbfA	-16.36	0.054483	188 -- 240	157 -- 199	ribosome-binding factor A	_____________187________________241\n_____________\|__________________\|\n5'-ACA...CGUGU_______CA_________AAGAA...CUG-3'\n______________CAGUGAG___AAUGAAAA\n______________\|\|:\|\|\|\|___\|\|\|\|\|\|\|\|\n______________GUUACUC___UUACUUUU\n3'-GUU...UGCCG_______UAC________GUUUA...GGA-5'\n_____________\|__________________\|\n_____________200________________156\n	234 -- 240	157 -- 163	-12.7883 kcal/mol	2.00785 kcal/mol	0.933292 kcal/mol	187 241@LB@ \| \|@LB@5'-ACA...CGUGU CA AAGAA...CUG-3'@LB@ CAGUGAG AAUGAAAA@LB@ \|\|:\|\|\|\| \|\|\|\|\|\|\|\|@LB@ GUUACUC UUACUUUU@LB@3'-GUU...UGCCG UAC GUUUA...GGA-5'@LB@ \| \|@LB@ 200 156@LB@@LB@@LB@Energy -16.36 kcal/mol@LB@Hybridization Energy -12.7883 kcal/mol@LB@Unfolding Energy - mRNA 2.00785 kcal/mol@LB@Unfolding Energy - sRNA 0.933292 kcal/mol@LB@@LB@Position - mRNA 188 -- 240@LB@Position - sRNA 157 -- 199@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 157 -- 163@LB@
105	0.02711	0.6733	lmo0347	N/A	-16.41	0.053410	62 -- 87	160 -- 186	dihydroxyacetone kinase	_____________61____________________________88\n_____________\|_____________________________\|\n5'-AUC...GUUGG__________ACU________________UUGGU...AUU-3'\n______________GAACUACGCG___UCGAUG_GAU__AUGA\n______________\|\|\|\|\|\|\|:\|\|___:\|:\|\|\|_\|\|\|__\|\|\|\|\n______________CUUGAUGUGC___GGUUAC_CUA__UACU\n3'-GUU...CUGUA__________C________U___CU____UUUGU...GGA-5'\n_____________\|_____________________________\|\n_____________187___________________________159\n	65 -- 71	177 -- 183	-23.7931 kcal/mol	4.23572 kcal/mol	9.57505 kcal/mol	61 88@LB@ \| \|@LB@5'-AUC...GUUGG ACU UUGGU...AUU-3'@LB@ GAACUACGCG UCGAUG GAU AUGA@LB@ \|\|\|\|\|\|\|:\|\| :\|:\|\|\| \|\|\| \|\|\|\|@LB@ CUUGAUGUGC GGUUAC CUA UACU@LB@3'-GUU...CUGUA C U CU UUUGU...GGA-5'@LB@ \| \|@LB@ 187 159@LB@@LB@@LB@Energy -16.41 kcal/mol@LB@Hybridization Energy -23.7931 kcal/mol@LB@Unfolding Energy - mRNA 4.23572 kcal/mol@LB@Unfolding Energy - sRNA 9.57505 kcal/mol@LB@@LB@Position - mRNA 62 -- 87@LB@Position - sRNA 160 -- 186@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 177 -- 183@LB@
106	0.02722	0.6733	lmo1616	N/A	-15.61	0.075454	67 -- 108	167 -- 211		_____________66______________________109\n_____________\|_______________________\|\n5'-AAG...AUAAC_____U_____GACU________AGGGC...UUU-3'\n______________GAACA_AUGUU____UGACUAGU\n______________:\|\|\|\|_\|\|\|\|:____\|\|\|\|\|\|\|\|\n______________UUUGU_UACAG____ACUGAUCA\n3'-GUU...ACAGA_____C_________________ACGGU...GGA-5'\n_____________\|_______________________\|\n_____________212_____________________166\n	90 -- 96	117 -- 123	-17.5219 kcal/mol	5.32799 kcal/mol	5.058 kcal/mol	66 109@LB@ \| \|@LB@5'-AAG...AUAAC U GACU AGGGC...UUU-3'@LB@ GAACA AUGUU UGACUAGU@LB@ :\|\|\|\| \|\|\|\|: \|\|\|\|\|\|\|\|@LB@ UUUGU UACAG ACUGAUCA@LB@3'-GUU...ACAGA C ACGGU...GGA-5'@LB@ \| \|@LB@ 212 166@LB@@LB@@LB@Energy -15.61 kcal/mol@LB@Hybridization Energy -17.5219 kcal/mol@LB@Unfolding Energy - mRNA 5.32799 kcal/mol@LB@Unfolding Energy - sRNA 5.058 kcal/mol@LB@@LB@Position - mRNA 67 -- 108@LB@Position - sRNA 167 -- 211@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 117 -- 123@LB@
107	0.02737	0.6733	lmo1575	N/A	-17.23	0.037041	80 -- 118	156 -- 205		_____________79______119\n_____________\|_______\|\n5'-GUA...UCACA_______C-3'\n______________AAUGGGG\n______________\|\|:\|\|\|:\n______________UUGCCCU\n3'-GUU...ACGUA_______ACUCC...GGA-5'\n_____________\|_______\|\n_____________206_____155\n	293 -- 299	201 -- 207	-8.20946 kcal/mol	0.611387 kcal/mol	0.326294 kcal/mol	79 119@LB@ \| \|@LB@5'-GUA...UCACA C-3'@LB@ AAUGGGG@LB@ \|\|:\|\|\|:@LB@ UUGCCCU@LB@3'-GUU...ACGUA ACUCC...GGA-5'@LB@ \| \|@LB@ 206 155@LB@@LB@@LB@Energy -17.23 kcal/mol@LB@Hybridization Energy -8.20946 kcal/mol@LB@Unfolding Energy - mRNA 0.611387 kcal/mol@LB@Unfolding Energy - sRNA 0.326294 kcal/mol@LB@@LB@Position - mRNA 80 -- 118@LB@Position - sRNA 156 -- 205@LB@Position Seed - mRNA 293 -- 299@LB@Position Seed - sRNA 201 -- 207@LB@
108	0.02743	0.6733	lmo2330	N/A	-16.53	0.050546	195 -- 214	191 -- 207		_____________194_________215\n_____________\|___________\|\n5'-ACG...GAGAA___________CAAGU...UUU-3'\n______________AGCGGGAUGAG\n______________\|:\|\|\|\|\|\|\|\|\|\n______________UUGCCCUACUC\n3'-GUU...ACGUA___________CGUAU...GGA-5'\n_____________\|___________\|\n_____________208_________190\n	199 -- 205	197 -- 203	-18.6004 kcal/mol	2.16538 kcal/mol	0.386761 kcal/mol	194 215@LB@ \| \|@LB@5'-ACG...GAGAA CAAGU...UUU-3'@LB@ AGCGGGAUGAG@LB@ \|:\|\|\|\|\|\|\|\|\|@LB@ UUGCCCUACUC@LB@3'-GUU...ACGUA CGUAU...GGA-5'@LB@ \| \|@LB@ 208 190@LB@@LB@@LB@Energy -16.53 kcal/mol@LB@Hybridization Energy -18.6004 kcal/mol@LB@Unfolding Energy - mRNA 2.16538 kcal/mol@LB@Unfolding Energy - sRNA 0.386761 kcal/mol@LB@@LB@Position - mRNA 195 -- 214@LB@Position - sRNA 191 -- 207@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 197 -- 203@LB@
109	0.02745	0.6733	lmo0843	N/A	-19.30	0.014041	79 -- 127	106 -- 151		_____________78____________________________________________________128\n_____________\|_____________________________________________________\|\n5'-CCG...GUCCA____C_________________________AUAAUUU__U_____________CCCUG...CUG-3'\n______________AGUG_UUUUGGUG_CU_GAC_GAUGUUUGA_______CU__UUGCCAUUUUAU\n______________\|:\|\|_\|\|\|\|\|\|\|\|_\|\|_:\|\|_\|\|\|\|\|:\|\|\|_______\|\|__\|\|\|\|\|\|\|\|:\|\|\|\n______________UUAC_AAAACCAC_GA_UUG_CUACAGACU_______GA__AACGGUAAGAUA\n3'-GUU...GUUUA____U________A__U___U__________________UC____________ACCUU...GGA-5'\n_____________\|_____________________________________________________\|\n_____________152___________________________________________________105\n	118 -- 124	109 -- 115	-38.6588 kcal/mol	9.72702 kcal/mol	18.7291 kcal/mol	78 128@LB@ \| \|@LB@5'-CCG...GUCCA C AUAAUUU U CCCUG...CUG-3'@LB@ AGUG UUUUGGUG CU GAC GAUGUUUGA CU UUGCCAUUUUAU@LB@ \|:\|\| \|\|\|\|\|\|\|\| \|\| :\|\| \|\|\|\|\|:\|\|\| \|\| \|\|\|\|\|\|\|\|:\|\|\|@LB@ UUAC AAAACCAC GA UUG CUACAGACU GA AACGGUAAGAUA@LB@3'-GUU...GUUUA U A U U UC ACCUU...GGA-5'@LB@ \| \|@LB@ 152 105@LB@@LB@@LB@Energy -19.30 kcal/mol@LB@Hybridization Energy -38.6588 kcal/mol@LB@Unfolding Energy - mRNA 9.72702 kcal/mol@LB@Unfolding Energy - sRNA 18.7291 kcal/mol@LB@@LB@Position - mRNA 79 -- 127@LB@Position - sRNA 106 -- 151@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 109 -- 115@LB@
110	0.02752	0.6733	lmo0057	N/A	-17.32	0.035592	178 -- 213	150 -- 185		_____________177_________________214\n_____________\|___________________\|\n5'-AAA...GCGGG_______CA__________AAGUA...AAG-3'\n______________AGUGGGA__UGAAUGAAGA\n______________\|:\|\|:\|\|__\|\|\|\|\|\|\|\|:\|\n______________UUACUCU__ACUUACUUUU\n3'-GUU...GCCGG___________________GUUUA...GGA-5'\n_____________\|___________________\|\n_____________186_________________149\n	201 -- 207	157 -- 163	-16.939 kcal/mol	2.5385 kcal/mol	0.930085 kcal/mol	177 214@LB@ \| \|@LB@5'-AAA...GCGGG CA AAGUA...AAG-3'@LB@ AGUGGGA UGAAUGAAGA@LB@ \|:\|\|:\|\| \|\|\|\|\|\|\|\|:\|@LB@ UUACUCU ACUUACUUUU@LB@3'-GUU...GCCGG GUUUA...GGA-5'@LB@ \| \|@LB@ 186 149@LB@@LB@@LB@Energy -17.32 kcal/mol@LB@Hybridization Energy -16.939 kcal/mol@LB@Unfolding Energy - mRNA 2.5385 kcal/mol@LB@Unfolding Energy - sRNA 0.930085 kcal/mol@LB@@LB@Position - mRNA 178 -- 213@LB@Position - sRNA 150 -- 185@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 157 -- 163@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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