Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
11	0.002745	0.4469	lmo0197	N/A	-21.05	0.005861	175 -- 258	154 -- 211	regulatory protein SpoVG	_____________174__________________259\n_____________\|____________________\|\n5'-UUA...AAACU_______A____________UUGCU...AUA-3'\n______________GAUG_GA_GAAUGAGAGCGA\n______________:\|\|\|_\|\|_\|\|\|\|\|\|:\|:\|:\|\n______________UUAC_CU_CUUACUUUUGUU\n3'-GUU...GCCGG____U__A____________UAUUA...GGA-5'\n_____________\|____________________\|\n_____________212__________________153\n	252 -- 258	154 -- 160	-17.4227 kcal/mol	2.96559 kcal/mol	0.959215 kcal/mol	174 259@LB@ \| \|@LB@5'-UUA...AAACU A UUGCU...AUA-3'@LB@ GAUG GA GAAUGAGAGCGA@LB@ :\|\|\| \|\| \|\|\|\|\|\|:\|:\|:\|@LB@ UUAC CU CUUACUUUUGUU@LB@3'-GUU...GCCGG U A UAUUA...GGA-5'@LB@ \| \|@LB@ 212 153@LB@@LB@@LB@Energy -21.05 kcal/mol@LB@Hybridization Energy -17.4227 kcal/mol@LB@Unfolding Energy - mRNA 2.96559 kcal/mol@LB@Unfolding Energy - sRNA 0.959215 kcal/mol@LB@@LB@Position - mRNA 175 -- 258@LB@Position - sRNA 154 -- 211@LB@Position Seed - mRNA 252 -- 258@LB@Position Seed - sRNA 154 -- 160@LB@
12	0.002933	0.4469	lmo1952	lysA	-19.68	0.011630	182 -- 245	154 -- 213	diaminopimelate decarboxylase	_____________181___________________________________________________________246\n_____________\|_____________________________________________________________\|\n5'-UUG...CUAUA_____AAGG______U__________________A______UG___ACA_______U____GCAGA...CGC-3'\n______________AUGUA____UGGGAU__GGCGU_GACGU___UUG_ACGGCU___GA___AUGAAUG_AAAU\n______________\|\|\|:\|____:\|\|\|\|\|__\|\|\|:\|_\|\|\|:\|___:\|:_\|\|\|\|\|:___\|\|___\|\|\|\|\|\|\|_\|\|\|:\n______________UACGU____GCCCUA__CCGUA_CUGUA___GAU_UGCCGG___CU___UACUUAC_UUUG\n3'-GUU...GCGGA_____AUU_______CU_____U_____CUU___G______UUA__C_________U____UUUAU...GGA-5'\n_____________\|_____________________________________________________________\|\n_____________214___________________________________________________________153\n	225 -- 231	161 -- 167	-39.877 kcal/mol	10.395 kcal/mol	19.5834 kcal/mol	181 246@LB@ \| \|@LB@5'-UUG...CUAUA AAGG U A UG ACA U GCAGA...CGC-3'@LB@ AUGUA UGGGAU GGCGU GACGU UUG ACGGCU GA AUGAAUG AAAU@LB@ \|\|\|:\| :\|\|\|\|\| \|\|\|:\| \|\|\|:\| :\|: \|\|\|\|\|: \|\| \|\|\|\|\|\|\| \|\|\|:@LB@ UACGU GCCCUA CCGUA CUGUA GAU UGCCGG CU UACUUAC UUUG@LB@3'-GUU...GCGGA AUU CU U CUU G UUA C U UUUAU...GGA-5'@LB@ \| \|@LB@ 214 153@LB@@LB@@LB@Energy -19.68 kcal/mol@LB@Hybridization Energy -39.877 kcal/mol@LB@Unfolding Energy - mRNA 10.395 kcal/mol@LB@Unfolding Energy - sRNA 19.5834 kcal/mol@LB@@LB@Position - mRNA 182 -- 245@LB@Position - sRNA 154 -- 213@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 161 -- 167@LB@
13	0.002947	0.4469	lmo0233	N/A	-20.88	0.006381	238 -- 276	156 -- 198	DNA repair protein RadA	_____________237_____________________________________________277\n_____________\|_______________________________________________\|\n5'-AAA...GUGUC______GU__________________AA___________________UCCGA...CNN-3'\n______________AGGCAU__GGAUAUGAAU____CGGC__AAUG_GAUGG__GAAAAUG\n______________\|\|\|\|\|\|__:\|\|:\|\|\|\|\|:____\|\|\|\|__\|\|\|\|_\|\|\|\|:__\|\|\|\|\|::\n______________UCCGUA__UCUGUACUUG____GCCG__UUAC_CUACU__CUUUUGU\n3'-GUU...CCUAC__________________AUGU____G_____U_____UA_______UUAUU...GGA-5'\n_____________\|_______________________________________________\|\n_____________199_____________________________________________155\n	271 -- 277	155 -- 161	-35.3246 kcal/mol	8.28493 kcal/mol	13.976 kcal/mol	237 277@LB@ \| \|@LB@5'-AAA...GUGUC GU AA UCCGA...CNN-3'@LB@ AGGCAU GGAUAUGAAU CGGC AAUG GAUGG GAAAAUG@LB@ \|\|\|\|\|\| :\|\|:\|\|\|\|\|: \|\|\|\| \|\|\|\| \|\|\|\|: \|\|\|\|\|::@LB@ UCCGUA UCUGUACUUG GCCG UUAC CUACU CUUUUGU@LB@3'-GUU...CCUAC AUGU G U UA UUAUU...GGA-5'@LB@ \| \|@LB@ 199 155@LB@@LB@@LB@Energy -20.88 kcal/mol@LB@Hybridization Energy -35.3246 kcal/mol@LB@Unfolding Energy - mRNA 8.28493 kcal/mol@LB@Unfolding Energy - sRNA 13.976 kcal/mol@LB@@LB@Position - mRNA 238 -- 276@LB@Position - sRNA 156 -- 198@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 155 -- 161@LB@
14	0.00304	0.4469	lmo1187	N/A	-19.26	0.014311	139 -- 172	153 -- 183	ethanolamine utilization protein EutQ	_____________138_______________________________173\n_____________\|_________________________________\|\n5'-UAA...GGUAU____________CC__AU______GA_______AGCAA...UUU-3'\n______________CUAUAUGGCUAA__GA__GAUGAA__AAAAUAA\n______________\|\|\|:\|:\|\|\|:\|\|__\|\|__\|\|\|\|\|\|__\|\|\|\|:\|\|\n______________GAUGUGCCGGUU__CU__CUACUU__UUUUGUU\n3'-GUU...UACUU____________A___________AC_______UAUUA...GGA-5'\n_____________\|_________________________________\|\n_____________184_______________________________152\n	165 -- 171	154 -- 160	-25.2263 kcal/mol	3.98561 kcal/mol	9.539 kcal/mol	138 173@LB@ \| \|@LB@5'-UAA...GGUAU CC AU GA AGCAA...UUU-3'@LB@ CUAUAUGGCUAA GA GAUGAA AAAAUAA@LB@ \|\|\|:\|:\|\|\|:\|\| \|\| \|\|\|\|\|\| \|\|\|\|:\|\|@LB@ GAUGUGCCGGUU CU CUACUU UUUUGUU@LB@3'-GUU...UACUU A AC UAUUA...GGA-5'@LB@ \| \|@LB@ 184 152@LB@@LB@@LB@Energy -19.26 kcal/mol@LB@Hybridization Energy -25.2263 kcal/mol@LB@Unfolding Energy - mRNA 3.98561 kcal/mol@LB@Unfolding Energy - sRNA 9.539 kcal/mol@LB@@LB@Position - mRNA 139 -- 172@LB@Position - sRNA 153 -- 183@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 154 -- 160@LB@
15	0.003165	0.4469	lmo0292	N/A	-21.41	0.004857	182 -- 249	155 -- 212	heat-shock protein htrA serine protease	_____________181_____________________________________________________________250\n_____________\|_______________________________________________________________\|\n5'-GAG...GCAGA____AAGGA__________G_G_AAGAAAAGAAUUUA_____AAACAGC______________CGCCG...AAC-3'\n______________GAGG_____AGACAUGGAC_A_A______________AAUGA_______GA_GAAUGAAAGCA\n______________\|\|\|\|_____\|\|\|\|\|\|\|:\|\|_\|_\|______________\|\|\|\|\|_______\|\|_\|\|\|\|\|\|\|\|:\|\|\n______________CUCC_____UCUGUACUUG_U_U______________UUACU_______CU_CUUACUUUUGU\n3'-GUU...CCCUA____GUA____________A_G_GCCGG_______________________A___________UUAUU...GGA-5'\n_____________\|_______________________________________________________________\|\n_____________213_____________________________________________________________154\n	243 -- 249	155 -- 161	-30.3296 kcal/mol	4.19045 kcal/mol	13.9822 kcal/mol	181 250@LB@ \| \|@LB@5'-GAG...GCAGA AAGGA G G AAGAAAAGAAUUUA AAACAGC CGCCG...AAC-3'@LB@ GAGG AGACAUGGAC A A AAUGA GA GAAUGAAAGCA@LB@ \|\|\|\| \|\|\|\|\|\|\|:\|\| \| \| \|\|\|\|\| \|\| \|\|\|\|\|\|\|\|:\|\|@LB@ CUCC UCUGUACUUG U U UUACU CU CUUACUUUUGU@LB@3'-GUU...CCCUA GUA A G GCCGG A UUAUU...GGA-5'@LB@ \| \|@LB@ 213 154@LB@@LB@@LB@Energy -21.41 kcal/mol@LB@Hybridization Energy -30.3296 kcal/mol@LB@Unfolding Energy - mRNA 4.19045 kcal/mol@LB@Unfolding Energy - sRNA 13.9822 kcal/mol@LB@@LB@Position - mRNA 182 -- 249@LB@Position - sRNA 155 -- 212@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 155 -- 161@LB@
16	0.003215	0.4469	lmo1403	mutS	-20.74	0.006867	183 -- 222	169 -- 209	DNA mismatch repair protein MutS	_____________182________________223\n_____________\|__________________\|\n5'-ACG...UGCUA__________________AGAAG...UGU-3'\n______________UAAUGAGA_GGAUGAAAA\n______________:\|\|\|\|\|\|\|_\|:\|\|\|\|\|\|\|\n______________GUUACUCU_CUUACUUUU\n3'-GUU...UGCCG________A_________GUUUA...GGA-5'\n_____________\|__________________\|\n_____________210________________168\n	172 -- 178	157 -- 163	-16.7756 kcal/mol	1.79354 kcal/mol	0.933292 kcal/mol	182 223@LB@ \| \|@LB@5'-ACG...UGCUA AGAAG...UGU-3'@LB@ UAAUGAGA GGAUGAAAA@LB@ :\|\|\|\|\|\|\| \|:\|\|\|\|\|\|\|@LB@ GUUACUCU CUUACUUUU@LB@3'-GUU...UGCCG A GUUUA...GGA-5'@LB@ \| \|@LB@ 210 168@LB@@LB@@LB@Energy -20.74 kcal/mol@LB@Hybridization Energy -16.7756 kcal/mol@LB@Unfolding Energy - mRNA 1.79354 kcal/mol@LB@Unfolding Energy - sRNA 0.933292 kcal/mol@LB@@LB@Position - mRNA 183 -- 222@LB@Position - sRNA 169 -- 209@LB@Position Seed - mRNA 172 -- 178@LB@Position Seed - sRNA 157 -- 163@LB@
17	0.003248	0.4469	lmo1161	N/A	-22.54	0.002685	189 -- 238	155 -- 204	ethanolamine utilization protein EutJ	_____________188___________________________________________________239\n_____________\|_____________________________________________________\|\n5'-GGU...GUAAG_____GGAAAA________U_______AACA________AC____________GCUUG...AAG-3'\n______________GAGGU______AUGGAUAU___UUACA____GCCAAUGA___GAAUGGAA_CA\n______________\|\|\|\|:______\|\|:\|\|:\|\|___:\|\|\|\|____\|\|\|\|\|\|\|\|___\|\|\|\|\|:\|\|_\|\|\n______________CUCCG______UAUCUGUA___GAUGU____CGGUUACU___CUUACUUU_GU\n3'-GUU...CCCUA___________________CUU_____GC__________CUA________U__UUAUU...GGA-5'\n_____________\|_____________________________________________________\|\n_____________205___________________________________________________154\n	230 -- 236	158 -- 164	-34.4634 kcal/mol	5.85583 kcal/mol	13.9822 kcal/mol	188 239@LB@ \| \|@LB@5'-GGU...GUAAG GGAAAA U AACA AC GCUUG...AAG-3'@LB@ GAGGU AUGGAUAU UUACA GCCAAUGA GAAUGGAA CA@LB@ \|\|\|\|: \|\|:\|\|:\|\| :\|\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|:\|\| \|\|@LB@ CUCCG UAUCUGUA GAUGU CGGUUACU CUUACUUU GU@LB@3'-GUU...CCCUA CUU GC CUA U UUAUU...GGA-5'@LB@ \| \|@LB@ 205 154@LB@@LB@@LB@Energy -22.54 kcal/mol@LB@Hybridization Energy -34.4634 kcal/mol@LB@Unfolding Energy - mRNA 5.85583 kcal/mol@LB@Unfolding Energy - sRNA 13.9822 kcal/mol@LB@@LB@Position - mRNA 189 -- 238@LB@Position - sRNA 155 -- 204@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 158 -- 164@LB@
18	0.003504	0.4469	lmo0129	N/A	-18.06	0.025228	187 -- 226	176 -- 203	N-acetylmuramoyl-L-alanine amidase	_____________186____________227\n_____________\|______________\|\n5'-UUU...GUCAG______________AAAAC...AAC-3'\n______________GAGA_GGAUGAGGA\n______________\|\|\|\|_\|:\|\|\|\|::\|\n______________CUCU_CUUACUUUU\n3'-GUU...GGUUA____A_________GUUUA...GGA-5'\n_____________\|______________\|\n_____________204____________175\n	189 -- 195	157 -- 163	-12.9517 kcal/mol	0.542108 kcal/mol	0.904482 kcal/mol	186 227@LB@ \| \|@LB@5'-UUU...GUCAG AAAAC...AAC-3'@LB@ GAGA GGAUGAGGA@LB@ \|\|\|\| \|:\|\|\|\|::\|@LB@ CUCU CUUACUUUU@LB@3'-GUU...GGUUA A GUUUA...GGA-5'@LB@ \| \|@LB@ 204 175@LB@@LB@@LB@Energy -18.06 kcal/mol@LB@Hybridization Energy -12.9517 kcal/mol@LB@Unfolding Energy - mRNA 0.542108 kcal/mol@LB@Unfolding Energy - sRNA 0.904482 kcal/mol@LB@@LB@Position - mRNA 187 -- 226@LB@Position - sRNA 176 -- 203@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 157 -- 163@LB@
19	0.003536	0.4469	lmo0321	N/A	-22.74	0.002408	114 -- 157	167 -- 207		_____________113____________________________________________158\n_____________\|______________________________________________\|\n5'-AUU...UGUUA________UA____G_AC__________GGA_____U_________AUGUU...AGG-3'\n______________AGCGGGAU__GAGG_A__UAGGUA_GAA____ACGG_CUAAUGAGA\n______________\|:\|\|\|\|\|\|__\|\|\|\|_\|__\|\|\|::\|_\|\|\|____\|\|\|\|_\|:\|\|\|\|\|\|\|\n______________UUGCCCUA__CUCC_U__AUCUGU_CUU____UGCC_GGUUACUCU\n3'-GUU...ACGUA______________G_________A___GAUG______________ACUUA...GGA-5'\n_____________\|______________________________________________\|\n_____________208____________________________________________166\n	151 -- 157	167 -- 173	-39.7249 kcal/mol	9.73371 kcal/mol	18.0488 kcal/mol	113 158@LB@ \| \|@LB@5'-AUU...UGUUA UA G AC GGA U AUGUU...AGG-3'@LB@ AGCGGGAU GAGG A UAGGUA GAA ACGG CUAAUGAGA@LB@ \|:\|\|\|\|\|\| \|\|\|\| \| \|\|\|::\| \|\|\| \|\|\|\| \|:\|\|\|\|\|\|\|@LB@ UUGCCCUA CUCC U AUCUGU CUU UGCC GGUUACUCU@LB@3'-GUU...ACGUA G A GAUG ACUUA...GGA-5'@LB@ \| \|@LB@ 208 166@LB@@LB@@LB@Energy -22.74 kcal/mol@LB@Hybridization Energy -39.7249 kcal/mol@LB@Unfolding Energy - mRNA 9.73371 kcal/mol@LB@Unfolding Energy - sRNA 18.0488 kcal/mol@LB@@LB@Position - mRNA 114 -- 157@LB@Position - sRNA 167 -- 207@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 167 -- 173@LB@
20	0.003655	0.4469	lmo1591	argC	-21.66	0.004270	183 -- 240	156 -- 208	N-acetyl-gamma-glutamyl-phosphate reductase	_____________182___________________________________________________________241\n_____________\|_____________________________________________________________\|\n5'-AAA...AUAAU________A________________AGUUUCAAU__U____G___C_______UG______UUGAG...GUU-3'\n______________UAACGGGG_GGGGU_UAGG_AUGAA_________CA_UGGC_CGA_GGGGU_A__GAGGAC\n______________\|\|\|\|\|\|\|:_\|:\|\|:_\|\|\|:_\|\|\|\|\|_________\|\|_:\|\|\|_\|:\|_\|:\|:\|_\|__\|\|::\|\|\n______________AUUGCCCU_CUCCG_AUCU_UACUU_________GU_GCCG_GUU_CUCUA_U__CUUUUG\n3'-GUU...UACGU________A_____U____G_____GAU_________________A_____C_UA______UUUAU...GGA-5'\n_____________\|_____________________________________________________________\|\n_____________209___________________________________________________________155\n	184 -- 190	201 -- 207	-40.8209 kcal/mol	11.7 kcal/mol	18.6528 kcal/mol	182 241@LB@ \| \|@LB@5'-AAA...AUAAU A AGUUUCAAU U G C UG UUGAG...GUU-3'@LB@ UAACGGGG GGGGU UAGG AUGAA CA UGGC CGA GGGGU A GAGGAC@LB@ \|\|\|\|\|\|\|: \|:\|\|: \|\|\|: \|\|\|\|\| \|\| :\|\|\| \|:\| \|:\|:\| \| \|\|::\|\|@LB@ AUUGCCCU CUCCG AUCU UACUU GU GCCG GUU CUCUA U CUUUUG@LB@3'-GUU...UACGU A U G GAU A C UA UUUAU...GGA-5'@LB@ \| \|@LB@ 209 155@LB@@LB@@LB@Energy -21.66 kcal/mol@LB@Hybridization Energy -40.8209 kcal/mol@LB@Unfolding Energy - mRNA 11.7 kcal/mol@LB@Unfolding Energy - sRNA 18.6528 kcal/mol@LB@@LB@Position - mRNA 183 -- 240@LB@Position - sRNA 156 -- 208@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 201 -- 207@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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