Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
81	0.02179	0.6733	lmo0087	N/A	-17.84	0.027970	185 -- 226	166 -- 207	lmo0087	_____________184____________________227\n_____________\|______________________\|\n5'-ACU...AUUAU___AU____G____________AACCU...AUC-3'\n______________UGA__UGGA_GAGAGCAAAUGA\n______________\|\|\|__\|\|:\|_\|\|:\|:\|\|\|\|\|:\|\n______________ACU__ACUU_CUUUUGUUUAUU\n3'-GUU...CGGUU___CU____A____________ACUAA...GGA-5'\n_____________\|______________________\|\n_____________208____________________165\n	195 -- 201	153 -- 159	-17.3998 kcal/mol	2.37482 kcal/mol	5.01319 kcal/mol	184 227@LB@ \| \|@LB@5'-ACU...AUUAU AU G AACCU...AUC-3'@LB@ UGA UGGA GAGAGCAAAUGA@LB@ \|\|\| \|\|:\| \|\|:\|:\|\|\|\|\|:\|@LB@ ACU ACUU CUUUUGUUUAUU@LB@3'-GUU...CGGUU CU A ACUAA...GGA-5'@LB@ \| \|@LB@ 208 165@LB@@LB@@LB@Energy -17.84 kcal/mol@LB@Hybridization Energy -17.3998 kcal/mol@LB@Unfolding Energy - mRNA 2.37482 kcal/mol@LB@Unfolding Energy - sRNA 5.01319 kcal/mol@LB@@LB@Position - mRNA 185 -- 226@LB@Position - sRNA 166 -- 207@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 153 -- 159@LB@
82	0.02188	0.6733	lmo0200	prfA	-17.77	0.028863	191 -- 222	162 -- 204	listeriolysin positive regulatory protein	_____________190______223\n_____________\|________\|\n5'-UCA...AUUGG________ACAUG...AUC-3'\n______________GGGAUGAG\n______________\|\|\|\|\|\|\|\|\n______________CCCUACUC\n3'-GUU...UAUUG________CGUAU...GGA-5'\n_____________\|________\|\n_____________205______161\n	192 -- 198	197 -- 203	-14.1607 kcal/mol	1.90446 kcal/mol	0.386531 kcal/mol	190 223@LB@ \| \|@LB@5'-UCA...AUUGG ACAUG...AUC-3'@LB@ GGGAUGAG@LB@ \|\|\|\|\|\|\|\|@LB@ CCCUACUC@LB@3'-GUU...UAUUG CGUAU...GGA-5'@LB@ \| \|@LB@ 205 161@LB@@LB@@LB@Energy -17.77 kcal/mol@LB@Hybridization Energy -14.1607 kcal/mol@LB@Unfolding Energy - mRNA 1.90446 kcal/mol@LB@Unfolding Energy - sRNA 0.386531 kcal/mol@LB@@LB@Position - mRNA 191 -- 222@LB@Position - sRNA 162 -- 204@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 197 -- 203@LB@
83	0.02274	0.6733	lmo1752	N/A	-17.06	0.039898	184 -- 210	151 -- 173	lmo1752	_____________183___________211\n_____________\|_____________\|\n5'-CUA...CAAGA_____________CGAUA...CAG-3'\n______________AAUGAGGUGAAUG\n______________\|\|\|\|\|\|:\|\|\|\|\|\|\n______________UUACUCUACUUAC\n3'-GUU...GCCGG_____________UUUUG...GGA-5'\n_____________\|_____________\|\n_____________174___________150\n	190 -- 196	161 -- 167	-16.9304 kcal/mol	1.40294 kcal/mol	0.838004 kcal/mol	183 211@LB@ \| \|@LB@5'-CUA...CAAGA CGAUA...CAG-3'@LB@ AAUGAGGUGAAUG@LB@ \|\|\|\|\|\|:\|\|\|\|\|\|@LB@ UUACUCUACUUAC@LB@3'-GUU...GCCGG UUUUG...GGA-5'@LB@ \| \|@LB@ 174 150@LB@@LB@@LB@Energy -17.06 kcal/mol@LB@Hybridization Energy -16.9304 kcal/mol@LB@Unfolding Energy - mRNA 1.40294 kcal/mol@LB@Unfolding Energy - sRNA 0.838004 kcal/mol@LB@@LB@Position - mRNA 184 -- 210@LB@Position - sRNA 151 -- 173@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 161 -- 167@LB@
84	0.02276	0.6733	lmo2467	N/A	-18.10	0.024762	166 -- 213	154 -- 198	chitin-binding protein	_____________165__________________________________________________214\n_____________\|____________________________________________________\|\n5'-UCA...CAAAA_____________________UGAAA__AA____CAUC______________CGAAG...AAC-3'\n______________AGGUAUA___AUGAACUAUAC_____GG__GGUG____AUGAA_GAAAAUGA\n______________\|\|\|:\|\|\|___\|\|\|\|\|\|\|\|:\|\|_____\|\|__::\|\|____\|\|\|\|\|_\|\|\|\|\|::\|\n______________UCCGUAU___UACUUGAUGUG_____CC__UUAC____UACUU_CUUUUGUU\n3'-GUU...CCUAC_______CUG__________________GG____UC_______A________UAUUA...GGA-5'\n_____________\|____________________________________________________\|\n_____________199__________________________________________________153\n	206 -- 212	155 -- 161	-31.7912 kcal/mol	6.75491 kcal/mol	13.9801 kcal/mol	165 214@LB@ \| \|@LB@5'-UCA...CAAAA UGAAA AA CAUC CGAAG...AAC-3'@LB@ AGGUAUA AUGAACUAUAC GG GGUG AUGAA GAAAAUGA@LB@ \|\|\|:\|\|\| \|\|\|\|\|\|\|\|:\|\| \|\| ::\|\| \|\|\|\|\| \|\|\|\|\|::\|@LB@ UCCGUAU UACUUGAUGUG CC UUAC UACUU CUUUUGUU@LB@3'-GUU...CCUAC CUG GG UC A UAUUA...GGA-5'@LB@ \| \|@LB@ 199 153@LB@@LB@@LB@Energy -18.10 kcal/mol@LB@Hybridization Energy -31.7912 kcal/mol@LB@Unfolding Energy - mRNA 6.75491 kcal/mol@LB@Unfolding Energy - sRNA 13.9801 kcal/mol@LB@@LB@Position - mRNA 166 -- 213@LB@Position - sRNA 154 -- 198@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 155 -- 161@LB@
85	0.0229	0.6733	lmo1788	N/A	-18.82	0.017630	177 -- 227	165 -- 211	transcriptional regulator	_____________176______________________________________________________228\n_____________\|________________________________________________________\|\n5'-UCU...AAAUG_______C______G____AA__G_____AAAA__A____CUGCUUAU________UUCAG...GGC-3'\n______________GAGGUGU_AGAUAU_GAAC__UG_ACGGU____GA_AUGG________GUGGGGAU\n______________\|\|\|\|::\|_\|\|\|:\|\|_\|\|\|\|__\|:_\|\|\|\|:____\|\|_\|\|\|:________:\|\|:::\|:\n______________CUCCGUA_UCUGUA_CUUG__AU_UGCCG____CU_UACU________UACUUUUG\n3'-GUU...CCCUA_______________________G_____GUUA__C____________________UUUAU...GGA-5'\n_____________\|________________________________________________________\|\n_____________212______________________________________________________164\n	238 -- 244	156 -- 162	-32.2408 kcal/mol	14.3369 kcal/mol	13.9689 kcal/mol	176 228@LB@ \| \|@LB@5'-UCU...AAAUG C G AA G AAAA A CUGCUUAU UUCAG...GGC-3'@LB@ GAGGUGU AGAUAU GAAC UG ACGGU GA AUGG GUGGGGAU@LB@ \|\|\|\|::\| \|\|\|:\|\| \|\|\|\| \|: \|\|\|\|: \|\| \|\|\|: :\|\|:::\|:@LB@ CUCCGUA UCUGUA CUUG AU UGCCG CU UACU UACUUUUG@LB@3'-GUU...CCCUA G GUUA C UUUAU...GGA-5'@LB@ \| \|@LB@ 212 164@LB@@LB@@LB@Energy -18.82 kcal/mol@LB@Hybridization Energy -32.2408 kcal/mol@LB@Unfolding Energy - mRNA 14.3369 kcal/mol@LB@Unfolding Energy - sRNA 13.9689 kcal/mol@LB@@LB@Position - mRNA 177 -- 227@LB@Position - sRNA 165 -- 211@LB@Position Seed - mRNA 238 -- 244@LB@Position Seed - sRNA 156 -- 162@LB@
86	0.02293	0.6733	lmo0853	N/A	-17.72	0.029549	77 -- 124	163 -- 214	SugE protein	_____________76__________________________________________________________125\n_____________\|___________________________________________________________\|\n5'-GAC...CGUUU________CUUCU______AC__________________________AUUUAU______ACGGA...UUU-3'\n______________UUUAUGCA_____UGAUGG__UGA_GUGUGGA________ACACGGC______GAUGAA\n______________::\|\|\|\|\|\|_____\|:\|:\|\|__\|\|\|_\|::\|:\|\|________\|\|\|\|\|\|\|______\|\|\|\|\|\|\n______________GGAUACGU_____AUUGCC__ACU_CGUAUCU________UGUGCCG______CUACUU\n3'-GUU...CCCGC___________________CU___C_______GUACUUGA_______GUUACU______ACUUU...GGA-5'\n_____________\|___________________________________________________________\|\n_____________215_________________________________________________________162\n	106 -- 112	175 -- 181	-38.3816 kcal/mol	10.3843 kcal/mol	19.6349 kcal/mol	76 125@LB@ \| \|@LB@5'-GAC...CGUUU CUUCU AC AUUUAU ACGGA...UUU-3'@LB@ UUUAUGCA UGAUGG UGA GUGUGGA ACACGGC GAUGAA@LB@ ::\|\|\|\|\|\| \|:\|:\|\| \|\|\| \|::\|:\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|@LB@ GGAUACGU AUUGCC ACU CGUAUCU UGUGCCG CUACUU@LB@3'-GUU...CCCGC CU C GUACUUGA GUUACU ACUUU...GGA-5'@LB@ \| \|@LB@ 215 162@LB@@LB@@LB@Energy -17.72 kcal/mol@LB@Hybridization Energy -38.3816 kcal/mol@LB@Unfolding Energy - mRNA 10.3843 kcal/mol@LB@Unfolding Energy - sRNA 19.6349 kcal/mol@LB@@LB@Position - mRNA 77 -- 124@LB@Position - sRNA 163 -- 214@LB@Position Seed - mRNA 106 -- 112@LB@Position Seed - sRNA 175 -- 181@LB@
87	0.02294	0.6733	lmo2765	N/A	-17.07	0.039744	188 -- 213	176 -- 199	PTS cellbiose transporter subunit IIA	_____________187_________________214\n_____________\|___________________\|\n5'-UUA...UAGAC________A__________GUUAU...AUU-3'\n______________AUGA_AUG_AAUGGAAACA\n______________\|\|\|\|_\|\|\|_\|\|\|\|:\|\|\|\|\|\n______________UACU_UAC_UUACUUUUGU\n3'-GUU...CCGGU____C______________UUAUU...GGA-5'\n_____________\|___________________\|\n_____________200_________________175\n	212 -- 218	155 -- 161	-13.3886 kcal/mol	2.47541 kcal/mol	0.935542 kcal/mol	187 214@LB@ \| \|@LB@5'-UUA...UAGAC A GUUAU...AUU-3'@LB@ AUGA AUG AAUGGAAACA@LB@ \|\|\|\| \|\|\| \|\|\|\|:\|\|\|\|\|@LB@ UACU UAC UUACUUUUGU@LB@3'-GUU...CCGGU C UUAUU...GGA-5'@LB@ \| \|@LB@ 200 175@LB@@LB@@LB@Energy -17.07 kcal/mol@LB@Hybridization Energy -13.3886 kcal/mol@LB@Unfolding Energy - mRNA 2.47541 kcal/mol@LB@Unfolding Energy - sRNA 0.935542 kcal/mol@LB@@LB@Position - mRNA 188 -- 213@LB@Position - sRNA 176 -- 199@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 155 -- 161@LB@
88	0.02298	0.6733	lmo0666	N/A	-13.39	0.184164	127 -- 173	156 -- 211		_____________126__________________________________________________________________________________174\n_____________\|____________________________________________________________________________________\|\n5'-AAG...AUUCA____A__GAACC______AA__C___AGU____AC___A__AAA_____GUUA______G_____GC_________________UCGUU...AUG-3'\n______________GCCA_CA______GGCGC__AU_GCA___AUGG__GGU_AG___AUAGA____GA___A_ACGGC_________GAAUGGAGAC\n______________\|\|\|\|_\|\|______\|\|\|\|\|__\|\|_\|\|\|___\|:\|\|__\|:\|_\|\|___\|\|\|\|\|____\|\|___\|_\|\|\|\|\|_________\|\|\|\|\|:\|:\|\|\n______________CGGU_GU______CCGCG__UA_CGU___UGCC__CUA_UC___UAUCU____CU___U_UGCCG_________CUUACUUUUG\n3'-GUU...CGCAA____A__ACAAGC_____GA______AU__________C__CG______GUA___UGA_G_____GUUACUCUA__________UUUAU...GGA-5'\n_____________\|____________________________________________________________________________________\|\n_____________212__________________________________________________________________________________155\n	167 -- 173	156 -- 162	-45.3275 kcal/mol	14.8697 kcal/mol	22.6898 kcal/mol	126 174@LB@ \| \|@LB@5'-AAG...AUUCA A GAACC AA C AGU AC A AAA GUUA G GC UCGUU...AUG-3'@LB@ GCCA CA GGCGC AU GCA AUGG GGU AG AUAGA GA A ACGGC GAAUGGAGAC@LB@ \|\|\|\| \|\| \|\|\|\|\| \|\| \|\|\| \|:\|\| \|:\| \|\| \|\|\|\|\| \|\| \| \|\|\|\|\| \|\|\|\|\|:\|:\|\|@LB@ CGGU GU CCGCG UA CGU UGCC CUA UC UAUCU CU U UGCCG CUUACUUUUG@LB@3'-GUU...CGCAA A ACAAGC GA AU C CG GUA UGA G GUUACUCUA UUUAU...GGA-5'@LB@ \| \|@LB@ 212 155@LB@@LB@@LB@Energy -13.39 kcal/mol@LB@Hybridization Energy -45.3275 kcal/mol@LB@Unfolding Energy - mRNA 14.8697 kcal/mol@LB@Unfolding Energy - sRNA 22.6898 kcal/mol@LB@@LB@Position - mRNA 127 -- 173@LB@Position - sRNA 156 -- 211@LB@Position Seed - mRNA 167 -- 173@LB@Position Seed - sRNA 156 -- 162@LB@
89	0.02343	0.6733	lmo1651	N/A	-15.38	0.082912	189 -- 217	147 -- 170	ABC transporter ATP-binding protein	_____________188___________________________218\n_____________\|_____________________________\|\n5'-GAA...AGAAG______UAGCA___________UG_____ACAAG...UUC-3'\n______________GGGGUG_____GAUGGAAGCGA__AAUGA\n______________\|:\|:\|\|_____:\|\|\|:\|\|:\|:\|__\|\|\|\|\|\n______________CUCUAC_____UUACUUUUGUU__UUACU\n3'-GUU...GGUUA______________________UA_____AAAAC...GGA-5'\n_____________\|_____________________________\|\n_____________171___________________________146\n	204 -- 210	154 -- 160	-21.1438 kcal/mol	4.28476 kcal/mol	5.93585 kcal/mol	188 218@LB@ \| \|@LB@5'-GAA...AGAAG UAGCA UG ACAAG...UUC-3'@LB@ GGGGUG GAUGGAAGCGA AAUGA@LB@ \|:\|:\|\| :\|\|\|:\|\|:\|:\| \|\|\|\|\|@LB@ CUCUAC UUACUUUUGUU UUACU@LB@3'-GUU...GGUUA UA AAAAC...GGA-5'@LB@ \| \|@LB@ 171 146@LB@@LB@@LB@Energy -15.38 kcal/mol@LB@Hybridization Energy -21.1438 kcal/mol@LB@Unfolding Energy - mRNA 4.28476 kcal/mol@LB@Unfolding Energy - sRNA 5.93585 kcal/mol@LB@@LB@Position - mRNA 189 -- 217@LB@Position - sRNA 147 -- 170@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 154 -- 160@LB@
90	0.02372	0.6733	lmo2712	N/A	-17.76	0.029098	218 -- 285	160 -- 210	gluconate kinase	_____________217________286\n_____________\|__________\|\n5'-AGA...AAAAU__________CUGUU...CGA-3'\n______________GCGGGGUGAG\n______________:\|\|\|\|:\|\|\|\|\n______________UGCCCUACUC\n3'-GUU...CGUAU__________CGUAU...GGA-5'\n_____________\|__________\|\n_____________211________159\n	190 -- 196	197 -- 203	-16.6296 kcal/mol	4.31289 kcal/mol	0.38676 kcal/mol	217 286@LB@ \| \|@LB@5'-AGA...AAAAU CUGUU...CGA-3'@LB@ GCGGGGUGAG@LB@ :\|\|\|\|:\|\|\|\|@LB@ UGCCCUACUC@LB@3'-GUU...CGUAU CGUAU...GGA-5'@LB@ \| \|@LB@ 211 159@LB@@LB@@LB@Energy -17.76 kcal/mol@LB@Hybridization Energy -16.6296 kcal/mol@LB@Unfolding Energy - mRNA 4.31289 kcal/mol@LB@Unfolding Energy - sRNA 0.38676 kcal/mol@LB@@LB@Position - mRNA 218 -- 285@LB@Position - sRNA 160 -- 210@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 197 -- 203@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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