Input and runtime details for job 6038639 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6038639 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:35:57 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:19:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:35:30 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:35:37 CET 2018
	Job Completed		@ Fri Feb 16 21:35:51 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6038639&toolName=CopraRNA ( 30 days expiry )

Description of the job

IhtA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000117 Chlamydia trachomatis D/UW-3/CX

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
165	0.06872	0.3424	ct_859	lytB	-7.21	0.129268	10 -- 22	19 -- 31	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	_____________9_____________23\n_____________\|_____________\|\n5'-UCG...UAUAA___________A_UAUCU...CUA-3'\n______________AGGCUAUUCCG_G\n______________\|:\|\|\|\|\|\|\|\|:_\|\n______________UUCGAUAAGGU_C\n3'-UUU...CAGUC___________A_CGCAA...GAA-5'\n_____________\|_____________\|\n_____________32____________18\n	14 -- 20	21 -- 27	-14.969 kcal/mol	2.89565 kcal/mol	4.85984 kcal/mol	9 23@LB@ \| \|@LB@5'-UCG...UAUAA A UAUCU...CUA-3'@LB@ AGGCUAUUCCG G@LB@ \|:\|\|\|\|\|\|\|\|: \|@LB@ UUCGAUAAGGU C@LB@3'-UUU...CAGUC A CGCAA...GAA-5'@LB@ \| \|@LB@ 32 18@LB@@LB@@LB@Energy -7.21 kcal/mol@LB@Hybridization Energy -14.969 kcal/mol@LB@Unfolding Energy - mRNA 2.89565 kcal/mol@LB@Unfolding Energy - sRNA 4.85984 kcal/mol@LB@@LB@Position - mRNA 10 -- 22@LB@Position - sRNA 19 -- 31@LB@Position Seed - mRNA 14 -- 20@LB@Position Seed - sRNA 21 -- 27@LB@
61	0.02387	0.29	ct_628	ispA	-7.51	0.111028	11 -- 52	17 -- 56	farnesyl diphosphate synthase	_____________10__________________________________________53\n_____________\|___________________________________________\|\n5'-UUC...GAGAG_G____AA___A_____GAAAA________________UA___AGAGG...UAG-3'\n______________C_UUGU__UAC_AAACG_____AGUUAGGAGCU_UUCC__GGU\n______________\|_:\|\|\|__\|\|\|_\|\|\|\|:_____\|\|\|:\|\|:\|\|\|\|_\|\|\|\|__\|\|:\n______________G_GACA__AUG_UUUGU_____UCAGUCUUCGA_AAGG__CCG\n3'-UUU...AAGGG_G____C__________GAUC____________U____UA___CAAUC...GAA-5'\n_____________\|___________________________________________\|\n_____________57__________________________________________16\n	36 -- 42	28 -- 34	-30.6817 kcal/mol	10.2369 kcal/mol	12.9359 kcal/mol	10 53@LB@ \| \|@LB@5'-UUC...GAGAG G AA A GAAAA UA AGAGG...UAG-3'@LB@ C UUGU UAC AAACG AGUUAGGAGCU UUCC GGU@LB@ \| :\|\|\| \|\|\| \|\|\|\|: \|\|\|:\|\|:\|\|\|\| \|\|\|\| \|\|:@LB@ G GACA AUG UUUGU UCAGUCUUCGA AAGG CCG@LB@3'-UUU...AAGGG G C GAUC U UA CAAUC...GAA-5'@LB@ \| \|@LB@ 57 16@LB@@LB@@LB@Energy -7.51 kcal/mol@LB@Hybridization Energy -30.6817 kcal/mol@LB@Unfolding Energy - mRNA 10.2369 kcal/mol@LB@Unfolding Energy - sRNA 12.9359 kcal/mol@LB@@LB@Position - mRNA 11 -- 52@LB@Position - sRNA 17 -- 56@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 28 -- 34@LB@
118	0.04846	0.3262	ct_515	rs8	-12.50	0.006183	13 -- 43	3 -- 33	30S ribosomal protein S8	_____________12_______________________________44\n_____________\|________________________________\|\n5'-GGA...GACGA___U________UU__________UG______AAAUC...UGC-3'\n______________GGA_GUUAUUCC__UGGU_UUAGA__AUCAGC\n______________:\|\|_\|:\|\|\|\|\|\|__\|\|\|:_\|\|\|\|\|__\|:\|\|:\|\n______________UCU_CGAUAAGG__ACCG_AAUCU__UGGUUG\n3'-UUU...CUCAG___U________U_____C_____UA______AA-5'\n_____________\|________________________________\|\n_____________34_______________________________2\n	18 -- 24	22 -- 28	-29.6343 kcal/mol	8.5463 kcal/mol	8.59196 kcal/mol	12 44@LB@ \| \|@LB@5'-GGA...GACGA U UU UG AAAUC...UGC-3'@LB@ GGA GUUAUUCC UGGU UUAGA AUCAGC@LB@ :\|\| \|:\|\|\|\|\|\| \|\|\|: \|\|\|\|\| \|:\|\|:\|@LB@ UCU CGAUAAGG ACCG AAUCU UGGUUG@LB@3'-UUU...CUCAG U U C UA AA-5'@LB@ \| \|@LB@ 34 2@LB@@LB@@LB@Energy -12.50 kcal/mol@LB@Hybridization Energy -29.6343 kcal/mol@LB@Unfolding Energy - mRNA 8.5463 kcal/mol@LB@Unfolding Energy - sRNA 8.59196 kcal/mol@LB@@LB@Position - mRNA 13 -- 43@LB@Position - sRNA 3 -- 33@LB@Position Seed - mRNA 18 -- 24@LB@Position Seed - sRNA 22 -- 28@LB@
119	0.04857	0.3262	ct_706	clpP_2	-8.60	0.061801	14 -- 27	22 -- 35	ATP-dependent Clp protease proteolytic subunit	_____________13_____________28\n_____________\|______________\|\n5'-CGA...AAGAU______________UUCUU...GGC-3'\n______________UUAGAGGUUGUUCC\n______________\|:\|\|\|:\|:\|:\|\|\|\|\n______________AGUCUUCGAUAAGG\n3'-UUU...AUCUC______________UACCG...GAA-5'\n_____________\|______________\|\n_____________36_____________21\n	16 -- 22	27 -- 33	-17.9181 kcal/mol	4.58788 kcal/mol	4.72627 kcal/mol	13 28@LB@ \| \|@LB@5'-CGA...AAGAU UUCUU...GGC-3'@LB@ UUAGAGGUUGUUCC@LB@ \|:\|\|\|:\|:\|:\|\|\|\|@LB@ AGUCUUCGAUAAGG@LB@3'-UUU...AUCUC UACCG...GAA-5'@LB@ \| \|@LB@ 36 21@LB@@LB@@LB@Energy -8.60 kcal/mol@LB@Hybridization Energy -17.9181 kcal/mol@LB@Unfolding Energy - mRNA 4.58788 kcal/mol@LB@Unfolding Energy - sRNA 4.72627 kcal/mol@LB@@LB@Position - mRNA 14 -- 27@LB@Position - sRNA 22 -- 35@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 27 -- 33@LB@
200	0.0896	0.3683	ct_487	yhhF	-5.19	0.334580	14 -- 20	79 -- 85	methyltransferase	_____________13______21\n_____________\|_______\|\n5'-ACG...UCCUC_______UAAUG...UUA-3'\n______________CGGUUAG\n______________\|\|\|:\|\|:\n______________GCCGAUU\n3'-UUU...UCUAU_______GAGGU...GAA-5'\n_____________\|_______\|\n_____________86______78\n	14 -- 20	79 -- 85	-7.01592 kcal/mol	0.727938 kcal/mol	1.09751 kcal/mol	13 21@LB@ \| \|@LB@5'-ACG...UCCUC UAAUG...UUA-3'@LB@ CGGUUAG@LB@ \|\|\|:\|\|:@LB@ GCCGAUU@LB@3'-UUU...UCUAU GAGGU...GAA-5'@LB@ \| \|@LB@ 86 78@LB@@LB@@LB@Energy -5.19 kcal/mol@LB@Hybridization Energy -7.01592 kcal/mol@LB@Unfolding Energy - mRNA 0.727938 kcal/mol@LB@Unfolding Energy - sRNA 1.09751 kcal/mol@LB@@LB@Position - mRNA 14 -- 20@LB@Position - sRNA 79 -- 85@LB@Position Seed - mRNA 14 -- 20@LB@Position Seed - sRNA 79 -- 85@LB@
42	0.01617	0.29	ct_610	N/A	-8.69	0.058803	15 -- 22	80 -- 87	virulence protein CADD	_____________14_______23\n_____________\|________\|\n5'-GAU...GCAGU________UUGUU...GGG-3'\n______________UGCGGCUG\n______________\|:\|\|\|\|\|:\n______________AUGCCGAU\n3'-UUU...CCUCU________UGAGG...GAA-5'\n_____________\|________\|\n_____________88_______79\n	16 -- 22	80 -- 86	-11.8172 kcal/mol	2.02516 kcal/mol	1.09752 kcal/mol	14 23@LB@ \| \|@LB@5'-GAU...GCAGU UUGUU...GGG-3'@LB@ UGCGGCUG@LB@ \|:\|\|\|\|\|:@LB@ AUGCCGAU@LB@3'-UUU...CCUCU UGAGG...GAA-5'@LB@ \| \|@LB@ 88 79@LB@@LB@@LB@Energy -8.69 kcal/mol@LB@Hybridization Energy -11.8172 kcal/mol@LB@Unfolding Energy - mRNA 2.02516 kcal/mol@LB@Unfolding Energy - sRNA 1.09752 kcal/mol@LB@@LB@Position - mRNA 15 -- 22@LB@Position - sRNA 80 -- 87@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 80 -- 86@LB@
21	0.0049	0.1918	ct_531	lpxA	-7.30	0.123824	16 -- 37	17 -- 34	acyl-[acyl-carrier-protein]--UDP-N- acetylglucosamine O-acyltransferase	_____________15_____________________38\n_____________\|______________________\|\n5'-UUG...GUAUC___A____AAA_______CA__CUGUU...CGC-3'\n______________CAG_AAGC___AUUCCGU__GC\n______________\|\|\|_\|\|\|\|___\|\|\|\|\|:\|__\|\|\n______________GUC_UUCG___UAAGGUA__CG\n3'-UUU...UCUCA________A_________C___CAAUC...GAA-5'\n_____________\|______________________\|\n_____________35_____________________16\n	27 -- 33	20 -- 26	-14.3796 kcal/mol	2.10386 kcal/mol	4.97809 kcal/mol	15 38@LB@ \| \|@LB@5'-UUG...GUAUC A AAA CA CUGUU...CGC-3'@LB@ CAG AAGC AUUCCGU GC@LB@ \|\|\| \|\|\|\| \|\|\|\|\|:\| \|\|@LB@ GUC UUCG UAAGGUA CG@LB@3'-UUU...UCUCA A C CAAUC...GAA-5'@LB@ \| \|@LB@ 35 16@LB@@LB@@LB@Energy -7.30 kcal/mol@LB@Hybridization Energy -14.3796 kcal/mol@LB@Unfolding Energy - mRNA 2.10386 kcal/mol@LB@Unfolding Energy - sRNA 4.97809 kcal/mol@LB@@LB@Position - mRNA 16 -- 37@LB@Position - sRNA 17 -- 34@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 20 -- 26@LB@
155	0.06493	0.3424	ct_355	N/A	-6.27	0.205473	16 -- 22	80 -- 86		_____________15______23\n_____________\|_______\|\n5'-GUU...UUCUG_______GGUCC...CCU-3'\n______________ACGGCUG\n______________\|\|\|\|\|\|:\n______________UGCCGAU\n3'-UUU...CUCUA_______UGAGG...GAA-5'\n_____________\|_______\|\n_____________87______79\n	16 -- 22	80 -- 86	-10.68 kcal/mol	3.341 kcal/mol	1.06449 kcal/mol	15 23@LB@ \| \|@LB@5'-GUU...UUCUG GGUCC...CCU-3'@LB@ ACGGCUG@LB@ \|\|\|\|\|\|:@LB@ UGCCGAU@LB@3'-UUU...CUCUA UGAGG...GAA-5'@LB@ \| \|@LB@ 87 79@LB@@LB@@LB@Energy -6.27 kcal/mol@LB@Hybridization Energy -10.68 kcal/mol@LB@Unfolding Energy - mRNA 3.341 kcal/mol@LB@Unfolding Energy - sRNA 1.06449 kcal/mol@LB@@LB@Position - mRNA 16 -- 22@LB@Position - sRNA 80 -- 86@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 80 -- 86@LB@
31	0.009434	0.2502	ct_824	N/A	-6.02	0.231369	20 -- 32	21 -- 33	Zn-dependent peptidase	_____________19____________33\n_____________\|_____________\|\n5'-GUC...UUUUA________G____GUAUG...CCG-3'\n______________GGAAGCUA_UCUA\n______________:\|\|\|\|\|\|\|_\|\|:\|\n______________UCUUCGAU_AGGU\n3'-UUU...CUCAG________A____ACCGC...GAA-5'\n_____________\|_____________\|\n_____________34____________20\n	21 -- 27	26 -- 32	-13.8347 kcal/mol	3.08274 kcal/mol	4.73034 kcal/mol	19 33@LB@ \| \|@LB@5'-GUC...UUUUA G GUAUG...CCG-3'@LB@ GGAAGCUA UCUA@LB@ :\|\|\|\|\|\|\| \|\|:\|@LB@ UCUUCGAU AGGU@LB@3'-UUU...CUCAG A ACCGC...GAA-5'@LB@ \| \|@LB@ 34 20@LB@@LB@@LB@Energy -6.02 kcal/mol@LB@Hybridization Energy -13.8347 kcal/mol@LB@Unfolding Energy - mRNA 3.08274 kcal/mol@LB@Unfolding Energy - sRNA 4.73034 kcal/mol@LB@@LB@Position - mRNA 20 -- 32@LB@Position - sRNA 21 -- 33@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 26 -- 32@LB@
57	0.02238	0.29	ct_132	N/A	-4.44	0.450895	20 -- 27	53 -- 60		_____________19_______28\n_____________\|________\|\n5'-GAU...AUGGA________AAAAA...ACA-3'\n______________UCUCCCCU\n______________\|\|:\|\|\|\|\|\n______________AGGGGGGA\n3'-UUU...ACUGA________CACAU...GAA-5'\n_____________\|________\|\n_____________61_______52\n	21 -- 27	53 -- 59	-12.9803 kcal/mol	2.08802 kcal/mol	6.45136 kcal/mol	19 28@LB@ \| \|@LB@5'-GAU...AUGGA AAAAA...ACA-3'@LB@ UCUCCCCU@LB@ \|\|:\|\|\|\|\|@LB@ AGGGGGGA@LB@3'-UUU...ACUGA CACAU...GAA-5'@LB@ \| \|@LB@ 61 52@LB@@LB@@LB@Energy -4.44 kcal/mol@LB@Hybridization Energy -12.9803 kcal/mol@LB@Unfolding Energy - mRNA 2.08802 kcal/mol@LB@Unfolding Energy - sRNA 6.45136 kcal/mol@LB@@LB@Position - mRNA 20 -- 27@LB@Position - sRNA 53 -- 60@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 53 -- 59@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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