Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM3600	-75	-47	-55	-49	1.65	GcvB	155	182	157	163	6.06	-15.65	-23.36	-1.51736	GUGGAAUAUUAAUUACAUCAGGAUGGAUA&UGUCUGUCCAUAGUGAUUAAUGUAGCAC	(((..(((((((((((....(((((((((&)))))))))...)))))))))))..)))	GUGGAAUAUUAAUUACAUCAGGAUGGAUAAAAAAGCAGGGACGCUUUUUAUUGUAAAGACAAACAAGGAAUAAUGAUGAGCAUCAGCGUAUUGGGUAUUGGCGACAACGUUGUCGAUAAAUACCUGCAUUCCGGCAUCAUGUACCCCGGC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0062731	0.7419678		1255123	fructoselysine 6-kinase
STM3236	25	40	34	40	5.36	GcvB	154	169	154	160	2.34	-15.54	-23.24	-1.5067	CAAUGUGGACAGGCAG&CUGUCUGUCCAUAGUG	((.(((((((((((((&))))))))))))).))	CCGGGCAGAAUAUCUGGCUAACAAUGCAGCGUCCAGCCCCUUAACAUAAAAGGAAGUAAAGAGAGGUUAAUAACGAUGAUUACGACCCGAACCGCCAAACAAUGUGGACAGGCAGACUACGGAUGGCUACAGGCCCGCUAUACCUUCUCC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0067362	0.7419678	yhaK	1254759	similar to E. coli orf hypothetical protein (AAC76141.1) Blastp hit to AAC76141.1 (233 aa) 84% identity in aa 1 - 233
STM2925	58	74	59	65	5.88	GcvB	153	170	163	169	2.49	-14.45	-22.82	-1.40102	UCACUAUGGCUGGCAGG&CCUGUCUGUCCAUAGUGA	(((((((((..((((((&))))))...)))))))))	UUUCCCGUGUUCACUUAAUGCGUUGCACGGUUCUUACGUCACUGGUUGUUAACCAAAUUUUUUCCUGGGGGAUAAAUGAGCGCGGGAAGCCCAAAAUUCACCGUUAGUCGCAUUGCGGCACUAUCACUGGUUUCACUAUGGCUGGCAGGU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0132805	0.7904009	nlpD	1254448	lipoprotein NlpD
STM1282	56	70	64	70	2.38	GcvB	36	50	36	42	5.56	-14.86	-22.8	-1.44077	CCCAGGAGAACGCGC&GCGUGUUCUGAUGGG	((((..(((((((((&)))))))))..))))	AACAAAAACAACAUCGUUCAUCAUAGUGCUUCGCAGUAAAUGUUCAUAUUGCACCAGGAGUGAGAAAUGGCCGAAAUGACUGAAGUCGCGAAGGGUGUCGCUACACACCAACGUUUAGUGGCUUUAUUAACCCAGGAGAACGCGCGCUAU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0103458	0.7883581	yeaK	1252800	putative cytoplasmic protein
STM0022	-74	-55	-63	-57	2.39	GcvB	127	147	129	135	5.89	-14.36	-22.64	-1.39229	UGUUGGGAGGCGAGAAAGGG&CCCUUUUUCACUUCCUGUACA	(((..(((((.(((((((((&))))))))).)))))...)))	CUGUUGGGAGGCGAGAAAGGGGCGUUACGCGCGUCACUGCGCGUAACAAACCGACGGCAUCAGGAUAUUACGCAGAUGAAAAAAAACGUACCGAUUUUCCUUCGAUUAUUGCUACUGCUGUCGGCGGCCGGCCUGUCGUUUGCCGCGCAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0140165	0.7904009	bcfB	1251540	fimbrial chaperone protein
STM3564	-44	-17	-25	-19	1.58	GcvB	65	90	67	73	6.67	-14.37	-22.62	-1.39326	GCAAAAAACAAAACAUAUAACAUCACGA&UUGUGAUGUUGUGUUGUUGUGUUUGC	((((...(((.(((((((((((((((((&)))))))))))))).))).)))))))	UUUAUAAAAAGUACAGUCUGCUUUUUAACCAGCAAAAAACAAAACAUAUAACAUCACGAAUGGGGAUACAGGCACAUGAAACGGAAAGCGAAAACAAUAAUCGCAGGGAUUGUUGCAUUAGCAGUCUCGCAGGGGGCAAUGGCAGAUGAU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.013933	0.7904009	livK	1255087	leucine ABC transporter subunit substrate-binding protein LivK
STM1524	-72	-45	-52	-46	2.44	GcvB	148	177	149	155	4.5	-15.64	-22.58	-1.51639	AUAUUAUUCAUUUUUCAGAAAGGGUAAA&UUUACCCUGUCUGUCCAUAGUGAUUAAUGU	((((((.(((((...((((.((((((((&)))))))).)))).....))))).))))))	UUAAUAUUAUUCAUUUUUCAGAAAGGGUAAAGGGCGUCAUGAUGGUACAACUCCCCUGAUAACGAGAGAAAGAACAUGACCAUGAUGACUGCAACACAGGCGUUAUCCGUGAAUCCUGCGACCGGCCAGACGCUGGCGGCAAUGCCCUGG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.006314	0.7419678	yneI	1253042	putative succinate-semialdehyde dehydrogenase
STM0283	41	60	54	60	3.62	GcvB	152	169	152	158	2.38	-16.47	-22.47	-1.59687	CAGUAUGUGGCGUGACGGGG&CCCUGUCUGUCCAUAGUG	((.((((.(((..(((((((&))))))).))))))).))	CGGUGGCGGAUAACGGCAGUAAAGCCGGGCGGGCGAAAAACCGUCGGGUGGAAAUUCUGGUAGUGGAGUGAAUGAAUGAUGAAAAAAUCAACCUAUGAUGUGUCUCAUCAUUCGGCAGUAUGUGGCGUGACGGGGGAUUAUUAUCGGAUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0036267	0.6975035		1251802
STM3810	-30	-11	-21	-15	3.57	GcvB	72	91	77	83	4.11	-14.7	-22.38	-1.42526	UGCAAAUAACGACAACCAAC&GUUGUGUUGUUGUGUUUGCA	((((((((.(((((((((((&)))).)))))))))))))))	GUACUGGUGAUGUUAUUUUUAGCAACGGAUCGCUAUAACUCAUCAUGCAAAUAACGACAACCAACAGAUGAAUGAGUGAUGAUAAAAAACGUGUGGUUAACGCUGAUAAUAUGGAAUGGGAUGCUUUUAUUAAGCGGUUGUUCAAGCGUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0114137	0.7904009	yidQ	1255337	putative outer membrane lipoprotein
STM2818	-20	-9	-20	-14	2.85	GcvB	153	164	158	164	1.39	-18.09	-22.33	-1.75394	UGGACAGGCGGG&CCUGUCUGUCCA	((((((((((((&))))))))))))	AAUUGUAGGUUUGGCAUGUAUGGCCGCCAUUACAGUUAUGCUUAUUAAAACGAUUUGGACAGGCGGGAGGUCAAUUUGAUCCCGGACGUAUCACAGGCUCUGGCCUGGCUGGAAAAACAUCCUCAGGCGUUAAAGGGGAUACAGCGCGGG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.001114	0.4343002	gshA	1254341	glutamate--cysteine ligase

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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