Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM2745	-21	11	-6	1	8.34	GcvB	36	61	44	50	5.69	-14.8	-28.83	-1.43495	UGAGCCGACUGAUACCCCAUCAUGAAACGCGC&GCGUGUUCUGAUGGGCUUUUGGCUUA	((((((((.......(((((((.((.((((((&)))))))))))))))...))))))))	AAUCCGUCAGUGGCAACAGCGCCGCACCGUCAUCCGUUAAUCCACGCUCAGGGAUGAGCCGACUGAUACCCCAUCAUGAAACGCGCGCCCUUUCUCUGUAAGCAGUCUCCGGACCGCACGCUCGAAGUGGUGAUCCUUGCCGGAAGCCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0107353	0.7904009		1254268
STM1257	-35	-12	-32	-26	7.95	GcvB	166	189	180	186	6.29	-13.92	-28.16	-1.34963	UGUGGUGGUGCUUAAUCGAUCGCU&AGUGAUUAAUGUAGCACCGCCAUA	((((((((((((..((..((((((&))))))..))..))))))))))))	CACUAUUAGUGUUUGCCGGAAAUACGAUUUCAGCCUGGCUUGUGGUGGUGCUUAAUCGAUCGCUGGAGCGUCCCGAUGCUCUAUAAUCCUGCCCCGACGUUGUUUCGUCUGCUCCUCUCCGUUACCUGUCUGCUGUUUAUUGCCGGAUAC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.018166	0.8371189		1252775	peptide ABC transporter permease
STM1033	42	70	60	66	5.67	GcvB	132	162	137	143	7.38	-15.02	-28.07	-1.45628	GACAGCAGGGAACGGACGCGGGGAGGGAA&UUUCACUUCCUGUACAUUUACCCUGUCUGUC	((((((((((........(((((((((((&)))).)))))))........))))).)))))	ACGAAAACCGUAAGCUGGGACUGGUAUUUGAUACUGAUCCGGCACAUGACCCGGGGGAACAGGAUAAUGCCGGAAGUGAAGACAACAGUGGCGGCGAUAAAAAUGCCGCCGGUGAUGACAGCAGGGAACGGACGCGGGGAGGGAAGCAGU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0093682	0.7883581		1252551	Gifsy-2 prophage Clp protease-like protein
STM1576	4	30	4	10	5.35	GcvB	61	86	80	86	7.74	-14.27	-27.36	-1.38356	ACACGACAAAACGAGAAUUAUAACCGU&ACGGUUGUGAUGUUGUGUUGUUGUGU	(((((((((.((((..(((((((((((&))))))))))).)))).)))))))))	UUCUCAAAAGAGCGCGUGACGCUCUGUCUCUUUUUUGUGUUUUUCUUCAAAUGAUAUGCGCAUGUGAGGGGUAAAAUGACACGACAAAACGAGAAUUAUAACCGUUAUCUCUUGAGUGACUGGCGACCCGAAAAUCCAGCCUUCUGGGAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0147888	0.7904009	narU	1253094	nitrate/nitrite transporter NarU
STM1871	-72	-28	-46	-40	5.98	GcvB	143	187	154	160	6.86	-14.41	-27.25	-1.39714	UGGCGAUACAGGACAGGAAAUUACAACGGACGGUGGAUCAGGUAC&GUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCA	(((((.......(((...((((((.((((((((.((.....((((&)))).....)))))))))).....))))))..))).....)))))	GGCUGGCGAUACAGGACAGGAAAUUACAACGGACGGUGGAUCAGGUACAACUUCAUAGCGCAAAACCACACGCCAGUGGCGCUUAUUGAAAAACUAAAAGGAACGGACUCAUUCACUGUGUCCGCAUGGAUUGAUCGCUAUGAGGUUUUA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0136032	0.7904009		1253392	putative phage integrase protein
STM3567	-51	-28	-45	-39	4.12	GcvB	63	87	75	81	7.58	-15.35	-27.05	-1.48828	AACACCACAACACACGUAACAACC&GGUUGUGAUGUUGUGUUGUUGUGUU	(((((.(((((((((((.((((((&)))))).)))).))))))).)))))	AAUCGAAAUAAAGUGCUGAACAAUAACACCACAACACACGUAACAACCAGAAUAAUGGGGAUUAUCAGGAUGAAUAUGAAGGGUAAAACGUUAUUGGCAGGAUGUAUCGCCCUGUCAUUAAGCCAUAUGGCAUUCGCCGACGACAUUAAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0076089	0.7711365	livJ	1255090	high-affinity branched-chain amino acid transporter
STM1822	43	69	62	68	6.4	GcvB	162	185	163	169	6.16	-14.36	-26.92	-1.39229	GUGGUGAUCUACAAUAAUACGCUGUGG&CCAUAGUGAUUAAUGUAGCACCGC	((((((..(((((.((((.((((((((&)))))))))))).)))))))))))	GUUACAGAGGUGACGGCGGCGCCACAUUGUGGCAGUCUUGAACCAUUCAAAUGAAAAGCAAAUGAGGAAUAAGUAAUGUCUAUCGUGCGUAUUGAUGCCGAAGAUCGUUGGUCAGACGUGGUGAUCUACAAUAAUACGCUGUGGUAUACC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0140165	0.7904009	yoaB	1253341	similar to E. coli orf hypothetical protein (AAC74879.1) Blastp hit to AAC74879.1 (130 aa) 92% identity in aa 17 - 130
STM1789	-10	11	5	11	3.84	GcvB	35	55	35	41	5.74	-17.32	-26.9	-1.67928	AGGAGCCCUCAUGAAUGCGCA&UGCGUGUUCUGAUGGGCUUUU	(((((((((((.(((((((((&)))))))))))).))))))))	CGAUACGGUGAUCUCCACCAUGAUCAAUGGCUAUCGCAGCCAUAAAUUCCCCAAACCGCACGACAAGGAGCCCUCAUGAAUGCGCAACGCGUAGUGGUGAUGGGGUUAGGAAACCUGCUGUGGGCCGAUGAAGGAUUCGGUAUCCGGGUC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0019866	0.598099		1253308	hydrogenase expression/formation protein
STM2793	-73	-48	-63	-57	4.05	GcvB	49	72	56	62	8.51	-14.28	-26.84	-1.38453	CGUUUCGAUUGUAGGCCGAAUAAGCC&GGCUUUUGGCUUACGGUUGUGAUG	(((..(((((((((((((((..((((&)))))))))))))))))))..)))	ACCGUUUCGAUUGUAGGCCGAAUAAGCCAGGCGCCAUCCGGUAAACGAGCGCAAUAACAAUAAAGAGGUUUUAGGAUGGGGCAAUUGUCAGAAUCACAUGCAUUAGGGGGCGGGCUGAAAUCACGCCACGUCACCAUGUUAUCGAUCGCC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0147012	0.7904009	gabP	1254316	GABA permease
STM0849	-26	-9	-22	-16	4.38	GcvB	140	162	152	158	4.45	-17.84	-26.67	-1.7297	GACAGGCAGGGUUUCAGG&CCUGUACAUUUACCCUGUCUGUC	((((((((((((..((((&)))).......))))))))))))	UGUUGCGCGGCCGCUGCAGGAUAAUGCGCUCUCGCGCUUAUAACAUUCAGACAGGCAGGGUUUCAGGAGAACAACAUGACGCAAUUUAUUACGCAUAAAUGGCUGGCGGCGCUGGGACUGGCCUCAUCCAUUGCGGCUUUUCCGGCCCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0013491	0.4351811	yliB	1252368	glutathione ABC transporter substrate-binding protein GsiB

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching