Input and runtime details for job 4935127 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_003197
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4935127 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:12:23 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:14:40 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:16:11 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:17:25 CET 2020
	Job Completed		@ Mon Jan 27 14:17:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4935127&toolName=IntaRNA ( 30 days expiry )

Description of the job

GcvB - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
STM0022	-74	-55	-63	-57	2.39	GcvB	127	147	129	135	5.89	-14.36	-22.64	-1.39229	UGUUGGGAGGCGAGAAAGGG&CCCUUUUUCACUUCCUGUACA	(((..(((((.(((((((((&))))))))).)))))...)))	CUGUUGGGAGGCGAGAAAGGGGCGUUACGCGCGUCACUGCGCGUAACAAACCGACGGCAUCAGGAUAUUACGCAGAUGAAAAAAAACGUACCGAUUUUCCUUCGAUUAUUGCUACUGCUGUCGGCGGCCGGCCUGUCGUUUGCCGCGCAG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0140165	0.7904009	bcfB	1251540	fimbrial chaperone protein
STM0069	-71	-52	-71	-65	3.48	GcvB	60	81	75	81	6.88	-14.87	-25.23	-1.44174	ACAGCACAUGCGCAACCGUA&UACGGUUGUGAUGUUGUGUUGU	((((((((..((((((((((&))))))))))....))))))))	GCCUACAGCACAUGCGCAACCGUAGACCGGAUAAGGUGUUUACGCCGCCAUCCGGCAAAAAACCGAGGUACAACUAUGAGCUAUUACGCCUUUGAAGGACUGAUCCCGGUGGUGCAUCCGGACGCGUUUGUCCACCCCAGCGCCGUGCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0102821	0.7883581	caiE	1251587	phenylacetic acid degradation protein PaaY
STM2793	-73	-48	-63	-57	4.05	GcvB	49	72	56	62	8.51	-14.28	-26.84	-1.38453	CGUUUCGAUUGUAGGCCGAAUAAGCC&GGCUUUUGGCUUACGGUUGUGAUG	(((..(((((((((((((((..((((&)))))))))))))))))))..)))	ACCGUUUCGAUUGUAGGCCGAAUAAGCCAGGCGCCAUCCGGUAAACGAGCGCAAUAACAAUAAAGAGGUUUUAGGAUGGGGCAAUUGUCAGAAUCACAUGCAUUAGGGGGCGGGCUGAAAUCACGCCACGUCACCAUGUUAUCGAUCGCC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0147012	0.7904009	gabP	1254316	GABA permease
STM3600	-75	-47	-55	-49	1.65	GcvB	155	182	157	163	6.06	-15.65	-23.36	-1.51736	GUGGAAUAUUAAUUACAUCAGGAUGGAUA&UGUCUGUCCAUAGUGAUUAAUGUAGCAC	(((..(((((((((((....(((((((((&)))))))))...)))))))))))..)))	GUGGAAUAUUAAUUACAUCAGGAUGGAUAAAAAAGCAGGGACGCUUUUUAUUGUAAAGACAAACAAGGAAUAAUGAUGAGCAUCAGCGUAUUGGGUAUUGGCGACAACGUUGUCGAUAAAUACCUGCAUUCCGGCAUCAUGUACCCCGGC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0062731	0.7419678		1255123	fructoselysine 6-kinase
STM3527	-66	-47	-54	-48	2.5	GcvB	139	163	140	146	4.45	-14.85	-21.8	-1.4398	GGACAACAGUUGGUGCAGGA&UCCUGUACAUUUACCCUGUCUGUCC	(((((((((...((((((((&)))))))).......)))).)))))	AGCCCGCAGGGACAACAGUUGGUGCAGGAUGUGGGAUAUGUGCCGCUGUAUCCGGCCGCGAAAUAAAGGGACUAAAUGGAAGAGGUUAAAUUAUAUGUGGAACGGGUUGGCAUAGACCCGGCCAGCGGCGCUGAUAUUCGUAUUGCACGG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0104098	0.7883581		1255050	similar to E. coli orf hypothetical protein (AAC73602.1) Blastp hit to AAC73602.1 (86 aa) 35% identity in aa 21 - 85
STM1571	-69	-46	-53	-47	3	GcvB	65	89	66	72	6.66	-15.24	-24.9	-1.47761	CAGUCACAAAGCAUGACAUCACAA&UUGUGAUGUUGUGUUGUUGUGUUUG	((..((((((((((((((((((((&))))))))))))))).)))))..))	UCAGGACAGUCACAAAGCAUGACAUCACAAAAAGCAACGCUAAUAGGGCUUGUUGCCAUCGUUCUGUGGAGCACGAUGGUUGGGUUAAUUCGCGGCGUCAGCGAAGGUCUCGGACCGGUAGGCGGCGCAGCCAUGAUCUAUUCAUUAAGC	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0081593	0.7799855	yddG	1253089	putative permease
STM1524	-72	-45	-52	-46	2.44	GcvB	148	177	149	155	4.5	-15.64	-22.58	-1.51639	AUAUUAUUCAUUUUUCAGAAAGGGUAAA&UUUACCCUGUCUGUCCAUAGUGAUUAAUGU	((((((.(((((...((((.((((((((&)))))))).)))).....))))).))))))	UUAAUAUUAUUCAUUUUUCAGAAAGGGUAAAGGGCGUCAUGAUGGUACAACUCCCCUGAUAACGAGAGAAAGAACAUGACCAUGAUGACUGCAACACAGGCGUUAUCCGUGAAUCCUGCGACCGGCCAGACGCUGGCGGCAAUGCCCUGG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.006314	0.7419678	yneI	1253042	putative succinate-semialdehyde dehydrogenase
STM3257	-68	-44	-63	-57	1.65	GcvB	46	81	70	76	9.96	-13.98	-25.59	-1.35545	ACAAAACAACAUUACGAAAGCCUAU&AUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGU	((((.((((((((((((((((((((&))))))))...........)))))))))))).))))	CAAAAUCACAAAACAACAUUACGAAAGCCUAUAAAUUCUUUCGAAGAAGAAAAACGAAAGAACUCGGAGAUAAAAGUGAAAGAAAUAAUUGCUCGUCAUAAAGCAGGAGAACAUCUGGGCAUCUGUUCAGUUUGUUCUGCUCAUCCCCUG	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0175423	0.8300455		1254780	D-tagatose-16-bisphosphate aldolase subunit GatZ
STM1715	-75	-43	-52	-46	2.7	GcvB	42	71	45	51	9.85	-16.36	-28.91	-1.5862	AUCAAUUUCCUGUGAGAAUAUUUGCCCAUCAGA&UCUGAUGGGCUUUUGGCUUACGGUUGUGAU	((((.....(((((((.......((((((((((&))))))))))......)))))))...))))	AUCAAUUUCCUGUGAGAAUAUUUGCCCAUCAGAAGUGCUCAGCGUAGACUAAUCUCCUUGUUUAAGGAGUAAAAAAUGCAUAAAGAGACGCAACCUAUCGAUCGCGAAACACUGUUGCUUGAAGCCAACAAAAUUAUUCGGGAACAUGAA	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.0039103	0.6975035	yciN	1253234	similar to E. coli orf hypothetical protein (AAC74355.1) Blastp hit to AAC74355.1 (83 aa) 92% identity in aa 1 - 83
STM3093	-66	-41	-47	-41	4.25	GcvB	72	101	72	78	11.77	-15.59	-31.61	-1.51155	GCGGACUGAUUGCAUACAGACACAAC&GUUGUGUUGUUGUGUUUGCAAUUGGUCUGC	((((((((((((((((((.(((((((&)))))))...)))))..)))))))))))))	CUUUCCUGAGCGGACUGAUUGCAUACAGACACAACUUUCGUUACGUUGCGGGCUCGUUUUGCUAUGGAGUGUGCAAUGUACCGUAAUUUUUCUUUUGCCGCUGCGUUGCUGGCCGCAGCGUUUUCAGGCCAGGCCCUGGCCGAUGGCAUU	ACUUCCUGAGCCGGAACGAAAAGUUUUAUCGGAAUGCGUGUUCUGAUGGGCUUUUGGCUUACGGUUGUGAUGUUGUGUUGUUGUGUUUGCAAUUGGUCUGCGAUUCAGACCACGGUAGCGAGACUACCCUUUUUCACUUCCUGUACAUUUACCCUGUCUGUCCAUAGUGAUUAAUGUAGCACCGCCAUAUUGCGGUGCUUU	0.006522	0.7419678	endA	1254616	deoxyribonuclease I

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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