Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
72	0.001216	0.05856	b3699	gyrB	-10.53	0.031588	1 -- 17	9 -- 25	DNA gyrase subunit B	______________1________________18\n______________\|________________\|\n___________5'-___UG____________AGCUU...GCA-3'\n______________AGA__AUUUUGCCUCUG\n______________\|\|\|__\|\|:\|\|:\|\|\|\|\|\|\n______________UCU__UAGAAUGGAGAC\n3'-AUU...UUCUA___UG____________AUGGGAUG-5'\n_____________\|_________________\|\n_____________26________________8\n	11 -- 17	9 -- 15	-19.3204 kcal/mol	1.07855 kcal/mol	7.71376 kcal/mol	1 18@LB@ \| \|@LB@ 5'- UG AGCUU...GCA-3'@LB@ AGA AUUUUGCCUCUG@LB@ \|\|\| \|\|:\|\|:\|\|\|\|\|\|@LB@ UCU UAGAAUGGAGAC@LB@3'-AUU...UUCUA UG AUGGGAUG-5'@LB@ \| \|@LB@ 26 8@LB@@LB@@LB@Energy -10.53 kcal/mol@LB@Hybridization Energy -19.3204 kcal/mol@LB@Unfolding Energy - mRNA 1.07855 kcal/mol@LB@Unfolding Energy - sRNA 7.71376 kcal/mol@LB@@LB@Position - mRNA 1 -- 17@LB@Position - sRNA 9 -- 25@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 9 -- 15@LB@
106	0.003365	0.1101	b0955	ycbZ	-10.52	0.031644	269 -- 288	48 -- 66	putative peptidase	_____________268__________________289\n_____________\|____________________\|\n5'-UUG...UUUGC_______C_________A__AAAAC...CUU-3'\n______________UCAGCCA_AUUUGAUUG_CG\n______________\|\|\|\|\|\|\|_\|\|::\|\|\|\|:_\|\|\n______________AGUCGGU_UAGGCUAAU_GC\n3'-AUU...UUAUA_______U____________ACUUC...AUG-5'\n_____________\|____________________\|\n_____________67___________________47\n	279 -- 285	50 -- 56	-21.5915 kcal/mol	3.60671 kcal/mol	7.48271 kcal/mol	268 289@LB@ \| \|@LB@5'-UUG...UUUGC C A AAAAC...CUU-3'@LB@ UCAGCCA AUUUGAUUG CG@LB@ \|\|\|\|\|\|\| \|\|::\|\|\|\|: \|\|@LB@ AGUCGGU UAGGCUAAU GC@LB@3'-AUU...UUAUA U ACUUC...AUG-5'@LB@ \| \|@LB@ 67 47@LB@@LB@@LB@Energy -10.52 kcal/mol@LB@Hybridization Energy -21.5915 kcal/mol@LB@Unfolding Energy - mRNA 3.60671 kcal/mol@LB@Unfolding Energy - sRNA 7.48271 kcal/mol@LB@@LB@Position - mRNA 269 -- 288@LB@Position - sRNA 48 -- 66@LB@Position Seed - mRNA 279 -- 285@LB@Position Seed - sRNA 50 -- 56@LB@
55	0.0008105	0.05184	b3262	yhdJ	-10.49	0.032282	53 -- 83	1 -- 34	DNA adenine methyltransferase SAM-dependent	_____________52_________________________________84\n_____________\|__________________________________\|\n5'-CCU...CUGCG____U____________A____GUG____G____GUAAA...GUG-3'\n______________CUGA__GAU_GGGCAUC__ACC___GUAC_CUGC\n______________\|\|\|\|__\|\|\|_\|::\|\|\|\|__\|\|\|___\|\|\|\|_\|\|:\|\n______________GACU__CUA_CUUGUAG__UGG___CAUG_GAUG\n3'-AUU...UUCCA____UU___U_______AA___AGA____G____-5'\n_____________\|_________________________________\|\n_____________35________________________________1\n	61 -- 67	18 -- 24	-29.8286 kcal/mol	10.2122 kcal/mol	9.14469 kcal/mol	52 84@LB@ \| \|@LB@5'-CCU...CUGCG U A GUG G GUAAA...GUG-3'@LB@ CUGA GAU GGGCAUC ACC GUAC CUGC@LB@ \|\|\|\| \|\|\| \|::\|\|\|\| \|\|\| \|\|\|\| \|\|:\|@LB@ GACU CUA CUUGUAG UGG CAUG GAUG@LB@3'-AUU...UUCCA UU U AA AGA G -5'@LB@ \| \|@LB@ 35 1@LB@@LB@@LB@Energy -10.49 kcal/mol@LB@Hybridization Energy -29.8286 kcal/mol@LB@Unfolding Energy - mRNA 10.2122 kcal/mol@LB@Unfolding Energy - sRNA 9.14469 kcal/mol@LB@@LB@Position - mRNA 53 -- 83@LB@Position - sRNA 1 -- 34@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 18 -- 24@LB@
122	0.004081	0.1177	b3940	metL	-10.48	0.032541	261 -- 280	41 -- 59	Bifunctional aspartokinase/homoserine dehydrogenase 2	_____________260__________________281\n_____________\|____________________\|\n5'-ACG...GCAGU_______CUG__________UUAUU...GCA-3'\n______________AGUCUGG___AUGUGAAGUG\n______________\|:\|\|:\|:___\|:\|\|\|\|\|\|\|:\n______________UUAGGCU___UGCACUUCAU\n3'-AUU...UCGGU_______AA___________UUUCC...AUG-5'\n_____________\|____________________\|\n_____________60___________________40\n	274 -- 280	41 -- 47	-17.3741 kcal/mol	3.59224 kcal/mol	3.31634 kcal/mol	260 281@LB@ \| \|@LB@5'-ACG...GCAGU CUG UUAUU...GCA-3'@LB@ AGUCUGG AUGUGAAGUG@LB@ \|:\|\|:\|: \|:\|\|\|\|\|\|\|:@LB@ UUAGGCU UGCACUUCAU@LB@3'-AUU...UCGGU AA UUUCC...AUG-5'@LB@ \| \|@LB@ 60 40@LB@@LB@@LB@Energy -10.48 kcal/mol@LB@Hybridization Energy -17.3741 kcal/mol@LB@Unfolding Energy - mRNA 3.59224 kcal/mol@LB@Unfolding Energy - sRNA 3.31634 kcal/mol@LB@@LB@Position - mRNA 261 -- 280@LB@Position - sRNA 41 -- 59@LB@Position Seed - mRNA 274 -- 280@LB@Position Seed - sRNA 41 -- 47@LB@
177	0.007672	0.1511	b2307	hisM	-10.48	0.032509	175 -- 237	1 -- 57	histidine ABC transporter permease	_____________174__________________________________________________________________238\n_____________\|____________________________________________________________________\|\n5'-CUC...GCUAC____UGGG___________________CCUGU___C___________AUGAAUA___GAA___G____UGUGG...CGG-3'\n______________UCCG____UGUGAAG___AGGG_CUGA_____GAU_GAA_AUCUUAC_______CUG___ACC_CUGC\n______________\|\|\|\|____:\|\|\|\|\|\|___\|:\|\|_\|\|\|\|_____\|\|\|_\|\|\|_\|\|\|\|\|\|\|_______\|\|\|___\|\|\|_\|\|:\|\n______________AGGC____GCACUUC___UUCC_GACU_____CUA_CUU_UAGAAUG_______GAC___UGG_GAUG\n3'-AUU...GGUUU____UAAU_______AUU____A____UU______U___G_______GA________A__________-5'\n_____________\|___________________________________________________________________\|\n_____________58__________________________________________________________________1\n	210 -- 216	14 -- 20	-38.5224 kcal/mol	15.358 kcal/mol	12.7026 kcal/mol	174 238@LB@ \| \|@LB@5'-CUC...GCUAC UGGG CCUGU C AUGAAUA GAA G UGUGG...CGG-3'@LB@ UCCG UGUGAAG AGGG CUGA GAU GAA AUCUUAC CUG ACC CUGC@LB@ \|\|\|\| :\|\|\|\|\|\| \|:\|\| \|\|\|\| \|\|\| \|\|\| \|\|\|\|\|\|\| \|\|\| \|\|\| \|\|:\|@LB@ AGGC GCACUUC UUCC GACU CUA CUU UAGAAUG GAC UGG GAUG@LB@3'-AUU...GGUUU UAAU AUU A UU U G GA A -5'@LB@ \| \|@LB@ 58 1@LB@@LB@@LB@Energy -10.48 kcal/mol@LB@Hybridization Energy -38.5224 kcal/mol@LB@Unfolding Energy - mRNA 15.358 kcal/mol@LB@Unfolding Energy - sRNA 12.7026 kcal/mol@LB@@LB@Position - mRNA 175 -- 237@LB@Position - sRNA 1 -- 57@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 14 -- 20@LB@
89	0.002377	0.09395	b4136	dsbD	-10.47	0.032769	211 -- 231	1 -- 22	thiol:disulfide interchange protein and activator of DsbC	_____________210____________________232\n_____________\|______________________\|\n5'-UGC...UCAAC_______UA_G___________UACUU...CGG-3'\n______________GCAUCUU__C_CUG_AUCCUGC\n______________:\|\|\|\|\|\|__\|_\|\|\|_\|:\|\|\|:\|\n______________UGUAGAA__G_GAC_UGGGAUG\n3'-AUU...UAUCU_______UG_A___A_______-5'\n_____________\|_____________________\|\n_____________23____________________1\n	225 -- 231	1 -- 7	-23.5098 kcal/mol	5.0672 kcal/mol	7.99236 kcal/mol	210 232@LB@ \| \|@LB@5'-UGC...UCAAC UA G UACUU...CGG-3'@LB@ GCAUCUU C CUG AUCCUGC@LB@ :\|\|\|\|\|\| \| \|\|\| \|:\|\|\|:\|@LB@ UGUAGAA G GAC UGGGAUG@LB@3'-AUU...UAUCU UG A A -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -10.47 kcal/mol@LB@Hybridization Energy -23.5098 kcal/mol@LB@Unfolding Energy - mRNA 5.0672 kcal/mol@LB@Unfolding Energy - sRNA 7.99236 kcal/mol@LB@@LB@Position - mRNA 211 -- 231@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 1 -- 7@LB@
11	3.674e-05	0.01134	b2099	yegU	-10.44	0.033453	166 -- 229	3 -- 59	ADP-ribosylglycohydrolase family protein	_____________165_________________________________________________________________230\n_____________\|___________________________________________________________________\|\n5'-GUG...UCGAU____G____AU____U___CAC______AAGUUAAA_____AC________AU______G_______UUAUG...CAC-3'\n______________GAUC_CGAU__UGCG_UGA___AAGGGG________UGAAA___AGAACGU____UCUC_GUGCUCU\n______________:\|\|\|_\|\|\|\|__\|:\|\|_\|\|\|___\|\|::\|\|________\|\|\|\|\|___\|\|\|\|\|:\|____:\|\|\|_\|\|:\|:\|\|\n______________UUAG_GCUA__AUGC_ACU___UUUUCC________ACUUU___UCUUGUA____GGAG_CAUGGGA\n3'-AUU...UCGGU___________________UCA______AG___________CUA_______GAAU____A_______UG-5'\n_____________\|___________________________________________________________________\|\n_____________60__________________________________________________________________2\n	223 -- 229	3 -- 9	-36.2809 kcal/mol	13.1575 kcal/mol	12.7026 kcal/mol	165 230@LB@ \| \|@LB@5'-GUG...UCGAU G AU U CAC AAGUUAAA AC AU G UUAUG...CAC-3'@LB@ GAUC CGAU UGCG UGA AAGGGG UGAAA AGAACGU UCUC GUGCUCU@LB@ :\|\|\| \|\|\|\| \|:\|\| \|\|\| \|\|::\|\| \|\|\|\|\| \|\|\|\|\|:\| :\|\|\| \|\|:\|:\|\|@LB@ UUAG GCUA AUGC ACU UUUUCC ACUUU UCUUGUA GGAG CAUGGGA@LB@3'-AUU...UCGGU UCA AG CUA GAAU A UG-5'@LB@ \| \|@LB@ 60 2@LB@@LB@@LB@Energy -10.44 kcal/mol@LB@Hybridization Energy -36.2809 kcal/mol@LB@Unfolding Energy - mRNA 13.1575 kcal/mol@LB@Unfolding Energy - sRNA 12.7026 kcal/mol@LB@@LB@Position - mRNA 166 -- 229@LB@Position - sRNA 3 -- 59@LB@Position Seed - mRNA 223 -- 229@LB@Position Seed - sRNA 3 -- 9@LB@
76	0.001511	0.06993	b3465	rsmD	-10.43	0.033857	227 -- 244	49 -- 65	16S rRNA m(2)G966 methyltransferase SAM-dependent	_____________226________________245\n_____________\|__________________\|\n5'-UUG...GGCAG____________C_____UGGCG...GCC-3'\n______________CGGCCAAAUCCG_AUUAU\n______________\|:\|\|\|\|\|\|\|\|\|\|_\|\|\|\|:\n______________GUCGGUUUAGGC_UAAUG\n3'-AUU...UAUAA__________________CACUU...AUG-5'\n_____________\|__________________\|\n_____________66_________________48\n	232 -- 238	54 -- 60	-22.129 kcal/mol	5.5281 kcal/mol	6.19707 kcal/mol	226 245@LB@ \| \|@LB@5'-UUG...GGCAG C UGGCG...GCC-3'@LB@ CGGCCAAAUCCG AUUAU@LB@ \|:\|\|\|\|\|\|\|\|\|\| \|\|\|\|:@LB@ GUCGGUUUAGGC UAAUG@LB@3'-AUU...UAUAA CACUU...AUG-5'@LB@ \| \|@LB@ 66 48@LB@@LB@@LB@Energy -10.43 kcal/mol@LB@Hybridization Energy -22.129 kcal/mol@LB@Unfolding Energy - mRNA 5.5281 kcal/mol@LB@Unfolding Energy - sRNA 6.19707 kcal/mol@LB@@LB@Position - mRNA 227 -- 244@LB@Position - sRNA 49 -- 65@LB@Position Seed - mRNA 232 -- 238@LB@Position Seed - sRNA 54 -- 60@LB@
70	0.001199	0.05856	b0954	fabA	-10.37	0.035200	228 -- 254	4 -- 31	beta-hydroxydecanoyl thioester dehydrase	_____________227__________________________255\n_____________\|____________________________\|\n5'-GUG...AUACA_________C_U___________GU_G_GGUGA...ACA-3'\n______________AAAGA_AGA_C_UCUUGCCUCUG__C_C\n______________\|\|\|\|\|_\|\|\|_\|_\|\|\|\|:\|\|\|\|\|\|__\|_\|\n______________UUUCU_UCU_G_AGAAUGGAGAC__G_G\n3'-AUU...CAGAC_____A___U_U___________AU_G_AUG-5'\n_____________\|____________________________\|\n_____________32___________________________3\n	243 -- 249	9 -- 15	-26.2676 kcal/mol	7.86183 kcal/mol	8.05676 kcal/mol	227 255@LB@ \| \|@LB@5'-GUG...AUACA C U GU G GGUGA...ACA-3'@LB@ AAAGA AGA C UCUUGCCUCUG C C@LB@ \|\|\|\|\| \|\|\| \| \|\|\|\|:\|\|\|\|\|\| \| \|@LB@ UUUCU UCU G AGAAUGGAGAC G G@LB@3'-AUU...CAGAC A U U AU G AUG-5'@LB@ \| \|@LB@ 32 3@LB@@LB@@LB@Energy -10.37 kcal/mol@LB@Hybridization Energy -26.2676 kcal/mol@LB@Unfolding Energy - mRNA 7.86183 kcal/mol@LB@Unfolding Energy - sRNA 8.05676 kcal/mol@LB@@LB@Position - mRNA 228 -- 254@LB@Position - sRNA 4 -- 31@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 9 -- 15@LB@
83	0.001985	0.08414	b1901	araF	-10.36	0.035498	164 -- 171	1 -- 8	L-arabinose ABC transporter periplasmic binding protein	_____________163______172\n_____________\|________\|\n5'-UCC...GUUUU________ACAAA...AGC-3'\n______________UGCCCUAC\n______________\|:\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	165 -- 171	1 -- 7	-13.292 kcal/mol	0.840759 kcal/mol	2.11603 kcal/mol	163 172@LB@ \| \|@LB@5'-UCC...GUUUU ACAAA...AGC-3'@LB@ UGCCCUAC@LB@ \|:\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -10.36 kcal/mol@LB@Hybridization Energy -13.292 kcal/mol@LB@Unfolding Energy - mRNA 0.840759 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 164 -- 171@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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