Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
132	0.0212	0.526	b2455	eutE	-9.17	0.140915	224 -- 235	1 -- 12	aldehyde oxidoreductase ethanolamine utilization protein		_____________223__________236\n_____________\|____________\|\n5'-GGU...GAACA________A___GGUAC...UCG-3'\n______________GGUGGUGA_AGC\n______________::\|\|:\|\|\|_\|\|\|\n______________UUACUACU_UCG\n3'-UUU...UUCAA________A___-5'\n_____________\|___________\|\n_____________13__________1\n	225 -- 231	5 -- 11	-12.5804 kcal/mol	3.3751 kcal/mol	0.0394463 kcal/mol	223 236@LB@ \| \|@LB@5'-GGU...GAACA A GGUAC...UCG-3'@LB@ GGUGGUGA AGC@LB@ ::\|\|:\|\|\| \|\|\|@LB@ UUACUACU UCG@LB@3'-UUU...UUCAA A -5'@LB@ \| \|@LB@ 13 1@LB@@LB@@LB@Energy -9.17 kcal/mol@LB@Hybridization Energy -12.5804 kcal/mol@LB@Unfolding Energy - mRNA 3.3751 kcal/mol@LB@Unfolding Energy - sRNA 0.0394463 kcal/mol@LB@@LB@Position - mRNA 224 -- 235@LB@Position - sRNA 1 -- 12@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 5 -- 11@LB@
134	0.02184	0.526	b0034	caiF	-11.03	0.059446	251 -- 259	1 -- 9	cai operon transcriptional activator		_____________250_______260\n_____________\|_________\|\n5'-UGA...GAAUG_________UGAAA...AUU-3'\n______________GAUGAUGGC\n______________\|\|\|\|\|\|:\|\|\n______________CUACUAUCG\n3'-UUU...AAUUA_________-5'\n_____________\|________\|\n_____________10_______1\n	253 -- 259	1 -- 7	-13.9707 kcal/mol	2.91573 kcal/mol	0.0200155 kcal/mol	250 260@LB@ \| \|@LB@5'-UGA...GAAUG UGAAA...AUU-3'@LB@ GAUGAUGGC@LB@ \|\|\|\|\|\|:\|\|@LB@ CUACUAUCG@LB@3'-UUU...AAUUA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -13.9707 kcal/mol@LB@Unfolding Energy - mRNA 2.91573 kcal/mol@LB@Unfolding Energy - sRNA 0.0200155 kcal/mol@LB@@LB@Position - mRNA 251 -- 259@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 1 -- 7@LB@
135	0.02191	0.526	b1342	ydaN	-17.19	0.001818	129 -- 197	1 -- 58	putative Zn(II) transporter		_____________128______________________________________________________________________198\n_____________\|________________________________________________________________________\|\n5'-CGU...GUAAU_______UG_______GCGUAACAGGGC______GCUA____UACA________UGC___________A___ACUGU...AUG-3'\n______________GGGGUGA__AGAUGUU____________CAGAAG_____GAC______AAAUAA___GUUGAUGAUGG_GGC\n______________\|\|\|\|\|:\|__\|\|\|::\|\|____________\|\|\|\|\|:_____\|\|\|______\|\|\|\|\|\|___\|\|\|:\|\|\|\|\|\|:_:\|\|\n______________CCCCAUU__UCUGUAA____________GUCUUU_____CUG______UUUAUU___CAAUUACUACU_UCG\n3'-UUU...UUUAU_______UG_________________________ACUUA___CCAUUA______U_____________A___-5'\n_____________\|_______________________________________________________________________\|\n_____________59______________________________________________________________________1\n	187 -- 193	5 -- 11	-40.4273 kcal/mol	16.9018 kcal/mol	6.33758 kcal/mol	128 198@LB@ \| \|@LB@5'-CGU...GUAAU UG GCGUAACAGGGC GCUA UACA UGC A ACUGU...AUG-3'@LB@ GGGGUGA AGAUGUU CAGAAG GAC AAAUAA GUUGAUGAUGG GGC@LB@ \|\|\|\|\|:\| \|\|\|::\|\| \|\|\|\|\|: \|\|\| \|\|\|\|\|\| \|\|\|:\|\|\|\|\|\|: :\|\|@LB@ CCCCAUU UCUGUAA GUCUUU CUG UUUAUU CAAUUACUACU UCG@LB@3'-UUU...UUUAU UG ACUUA CCAUUA U A -5'@LB@ \| \|@LB@ 59 1@LB@@LB@@LB@Energy -17.19 kcal/mol@LB@Hybridization Energy -40.4273 kcal/mol@LB@Unfolding Energy - mRNA 16.9018 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 129 -- 197@LB@Position - sRNA 1 -- 58@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 5 -- 11@LB@
136	0.02238	0.526	b1883	cheB	-10.46	0.078180	185 -- 206	1 -- 23	fused chemotaxis regulator: protein-glutamate methylesterase in two-component regulatory system with CheA		_____________184______________________207\n_____________\|________________________\|\n5'-GGG...UGCGC_________A____C_________AAAAU...CGA-3'\n______________UAAGUAAGG_UUAA_GAUGA__GC\n______________\|\|\|:\|\|\|:\|_\|\|\|\|_\|\|\|\|\|__\|\|\n______________AUUUAUUUC_AAUU_CUACU__CG\n3'-UUU...CCAUU______________A_____AU__-5'\n_____________\|_______________________\|\n_____________24______________________1\n	187 -- 193	15 -- 21	-15.1436 kcal/mol	4.57462 kcal/mol	0.105066 kcal/mol	184 207@LB@ \| \|@LB@5'-GGG...UGCGC A C AAAAU...CGA-3'@LB@ UAAGUAAGG UUAA GAUGA GC@LB@ \|\|\|:\|\|\|:\| \|\|\|\| \|\|\|\|\| \|\|@LB@ AUUUAUUUC AAUU CUACU CG@LB@3'-UUU...CCAUU A AU -5'@LB@ \| \|@LB@ 24 1@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -15.1436 kcal/mol@LB@Unfolding Energy - mRNA 4.57462 kcal/mol@LB@Unfolding Energy - sRNA 0.105066 kcal/mol@LB@@LB@Position - mRNA 185 -- 206@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 15 -- 21@LB@
140	0.02319	0.526	b3615	waaH	-10.58	0.073933	178 -- 212	1 -- 47	LPS(HepIII)-glucuronic acid glycosyltransferase		_____________177______________________________________________213\n_____________\|________________________________________________\|\n5'-GAU...GGAUA____________C____U_______UU________________AC___ACCAA...GGA-3'\n______________AC_UUCAGAGGU____G_CGGUAAU___________AUGAUGA__AGC\n______________\|\|_\|\|\|\|\|\|::\|____\|_\|\|\|\|\|\|\|___________\|\|\|\|\|\|\|__\|\|\|\n______________UG_AAGUCUUUA____C_GCCAUUA___________UACUACU__UCG\n3'-UUU...UUGUC__U_________CUUA_U_______UUUAUUUCAAU_______A____-5'\n_____________\|_______________________________________________\|\n_____________48______________________________________________1\n	201 -- 207	5 -- 11	-24.9941 kcal/mol	8.09222 kcal/mol	6.32011 kcal/mol	177 213@LB@ \| \|@LB@5'-GAU...GGAUA C U UU AC ACCAA...GGA-3'@LB@ AC UUCAGAGGU G CGGUAAU AUGAUGA AGC@LB@ \|\| \|\|\|\|\|\|::\| \| \|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|@LB@ UG AAGUCUUUA C GCCAUUA UACUACU UCG@LB@3'-UUU...UUGUC U CUUA U UUUAUUUCAAU A -5'@LB@ \| \|@LB@ 48 1@LB@@LB@@LB@Energy -10.58 kcal/mol@LB@Hybridization Energy -24.9941 kcal/mol@LB@Unfolding Energy - mRNA 8.09222 kcal/mol@LB@Unfolding Energy - sRNA 6.32011 kcal/mol@LB@@LB@Position - mRNA 178 -- 212@LB@Position - sRNA 1 -- 47@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 5 -- 11@LB@
141	0.0236	0.526	b3339	tufA	-10.69	0.070202	69 -- 84	1 -- 19	translation elongation factor EF-Tu 1	b3980	_____________68__________________85\n_____________\|___________________\|\n5'-AAU...AUUCC___________________GCCGA...CCA-3'\n______________UGAAGU__AUGAUGA_AGC\n______________\|:\|\|\|\|__\|\|\|\|\|\|\|_\|\|\|\n______________AUUUCA__UACUACU_UCG\n3'-UUU...UAUUU______AU_______A___-5'\n_____________\|__________________\|\n_____________20_________________1\n	75 -- 81	5 -- 11	-13.5879 kcal/mol	2.83361 kcal/mol	0.0638423 kcal/mol	68 85@LB@ \| \|@LB@5'-AAU...AUUCC GCCGA...CCA-3'@LB@ UGAAGU AUGAUGA AGC@LB@ \|:\|\|\|\| \|\|\|\|\|\|\| \|\|\|@LB@ AUUUCA UACUACU UCG@LB@3'-UUU...UAUUU AU A -5'@LB@ \| \|@LB@ 20 1@LB@@LB@@LB@Energy -10.69 kcal/mol@LB@Hybridization Energy -13.5879 kcal/mol@LB@Unfolding Energy - mRNA 2.83361 kcal/mol@LB@Unfolding Energy - sRNA 0.0638423 kcal/mol@LB@@LB@Position - mRNA 69 -- 84@LB@Position - sRNA 1 -- 19@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 5 -- 11@LB@
142	0.02395	0.526	b0192	nlpE	-14.44	0.009771	167 -- 226	1 -- 55	lipoprotein involved with copper homeostasis and adhesion		_____________166_______________________________________________________________227\n_____________\|_________________________________________________________________\|\n5'-UCA...GCAAA___C_______G________AAAAAGAAG____G________AA_______C____A____C___GAUGG...CGC-3'\n______________GUG_GCAG_CG_UCGGGAAU_________GAAU_GAUGGUGA_____AAAG__GAU_GUGA_AGC\n______________\|\|:_:\|\|\|_\|:_\|\|:\|:\|\|\|_________\|\|\|\|_\|\|:\|\|\|:\|_____\|\|\|\|__:\|\|_:\|\|\|_\|\|\|\n______________CAU_UGUC_GU_AGUCUUUA_________CUUA_CUGCCAUU_____UUUC__UUA_UACU_UCG\n3'-UUU...AUCCC___U____U__A______________________________AUUUA____AA___C____A___-5'\n_____________\|________________________________________________________________\|\n_____________56_______________________________________________________________1\n	201 -- 207	24 -- 30	-31.0616 kcal/mol	10.2836 kcal/mol	6.33758 kcal/mol	166 227@LB@ \| \|@LB@5'-UCA...GCAAA C G AAAAAGAAG G AA C A C GAUGG...CGC-3'@LB@ GUG GCAG CG UCGGGAAU GAAU GAUGGUGA AAAG GAU GUGA AGC@LB@ \|\|: :\|\|\| \|: \|\|:\|:\|\|\| \|\|\|\| \|\|:\|\|\|:\| \|\|\|\| :\|\| :\|\|\| \|\|\|@LB@ CAU UGUC GU AGUCUUUA CUUA CUGCCAUU UUUC UUA UACU UCG@LB@3'-UUU...AUCCC U U A AUUUA AA C A -5'@LB@ \| \|@LB@ 56 1@LB@@LB@@LB@Energy -14.44 kcal/mol@LB@Hybridization Energy -31.0616 kcal/mol@LB@Unfolding Energy - mRNA 10.2836 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 167 -- 226@LB@Position - sRNA 1 -- 55@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 24 -- 30@LB@
143	0.02405	0.526	b3292	zntR	-11.17	0.055543	275 -- 285	1 -- 12	zntA gene transcriptional activator		_____________274__________286\n_____________\|____________\|\n5'-UGA...CAGCA____________AUGAA...CUG-3'\n______________GAUGAUGG_AGC\n______________:\|\|\|\|\|\|:_\|\|\|\n______________UUACUACU_UCG\n3'-UUU...UUCAA________A___-5'\n_____________\|___________\|\n_____________13__________1\n	276 -- 282	5 -- 11	-12.3439 kcal/mol	1.13072 kcal/mol	0.0394463 kcal/mol	274 286@LB@ \| \|@LB@5'-UGA...CAGCA AUGAA...CUG-3'@LB@ GAUGAUGG AGC@LB@ :\|\|\|\|\|\|: \|\|\|@LB@ UUACUACU UCG@LB@3'-UUU...UUCAA A -5'@LB@ \| \|@LB@ 13 1@LB@@LB@@LB@Energy -11.17 kcal/mol@LB@Hybridization Energy -12.3439 kcal/mol@LB@Unfolding Energy - mRNA 1.13072 kcal/mol@LB@Unfolding Energy - sRNA 0.0394463 kcal/mol@LB@@LB@Position - mRNA 275 -- 285@LB@Position - sRNA 1 -- 12@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 5 -- 11@LB@
147	0.02463	0.526	b2830	rppH	-8.72	0.170707	205 -- 218	1 -- 14	RNA pyrophosphohydrolase		_____________204____________219\n_____________\|______________\|\n5'-UUC...GGUGA_______C______UACCG...GGC-3'\n______________UUGAUGA_GAUGGC\n______________\|\|:\|\|\|\|_\|\|\|:\|\|\n______________AAUUACU_CUAUCG\n3'-UUU...AUUUC_______A______-5'\n_____________\|_____________\|\n_____________15____________1\n	205 -- 211	8 -- 14	-13.7427 kcal/mol	4.97796 kcal/mol	0.0473494 kcal/mol	204 219@LB@ \| \|@LB@5'-UUC...GGUGA C UACCG...GGC-3'@LB@ UUGAUGA GAUGGC@LB@ \|\|:\|\|\|\| \|\|\|:\|\|@LB@ AAUUACU CUAUCG@LB@3'-UUU...AUUUC A -5'@LB@ \| \|@LB@ 15 1@LB@@LB@@LB@Energy -8.72 kcal/mol@LB@Hybridization Energy -13.7427 kcal/mol@LB@Unfolding Energy - mRNA 4.97796 kcal/mol@LB@Unfolding Energy - sRNA 0.0473494 kcal/mol@LB@@LB@Position - mRNA 205 -- 218@LB@Position - sRNA 1 -- 14@LB@Position Seed - mRNA 205 -- 211@LB@Position Seed - sRNA 8 -- 14@LB@
149	0.02481	0.526	b2108	yehA	-13.80	0.014025	228 -- 241	1 -- 14	putative fimbrial-like adhesin protein		_____________227____________242\n_____________\|______________\|\n5'-GUU...UUAUA______________UAUGC...UUG-3'\n______________UUAAUGAUGAUGGU\n______________\|\|\|\|\|\|\|\|\|\|\|:\|:\n______________AAUUACUACUAUCG\n3'-UUU...AUUUC______________-5'\n_____________\|_____________\|\n_____________15____________1\n	235 -- 241	1 -- 7	-16.5389 kcal/mol	2.69058 kcal/mol	0.0473494 kcal/mol	227 242@LB@ \| \|@LB@5'-GUU...UUAUA UAUGC...UUG-3'@LB@ UUAAUGAUGAUGGU@LB@ \|\|\|\|\|\|\|\|\|\|\|:\|:@LB@ AAUUACUACUAUCG@LB@3'-UUU...AUUUC -5'@LB@ \| \|@LB@ 15 1@LB@@LB@@LB@Energy -13.80 kcal/mol@LB@Hybridization Energy -16.5389 kcal/mol@LB@Unfolding Energy - mRNA 2.69058 kcal/mol@LB@Unfolding Energy - sRNA 0.0473494 kcal/mol@LB@@LB@Position - mRNA 228 -- 241@LB@Position - sRNA 1 -- 14@LB@Position Seed - mRNA 235 -- 241@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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