Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
13	0.0005091	0.1286	b2062	wza	-15.65	0.004784	187 -- 207	5 -- 27	colanic acid export protein; outer membrane auxillary lipoprotein		_____________186_____________________208\n_____________\|_______________________\|\n5'-AAA...CCAAA____________C__________AAUCC...GCA-3'\n______________GUGAUAAAUAAA___AAUGAUGA\n______________\|\|:\|\|\|\|\|\|\|\|\|___\|\|\|\|\|\|\|\|\n______________CAUUAUUUAUUU___UUACUACU\n3'-UUU...ACUGC____________CAA________AUCG-5'\n_____________\|_______________________\|\n_____________28______________________4\n	201 -- 207	5 -- 11	-18.4073 kcal/mol	2.04721 kcal/mol	0.709121 kcal/mol	186 208@LB@ \| \|@LB@5'-AAA...CCAAA C AAUCC...GCA-3'@LB@ GUGAUAAAUAAA AAUGAUGA@LB@ \|\|:\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|@LB@ CAUUAUUUAUUU UUACUACU@LB@3'-UUU...ACUGC CAA AUCG-5'@LB@ \| \|@LB@ 28 4@LB@@LB@@LB@Energy -15.65 kcal/mol@LB@Hybridization Energy -18.4073 kcal/mol@LB@Unfolding Energy - mRNA 2.04721 kcal/mol@LB@Unfolding Energy - sRNA 0.709121 kcal/mol@LB@@LB@Position - mRNA 187 -- 207@LB@Position - sRNA 5 -- 27@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 5 -- 11@LB@
28	0.00198	0.2275	b2821	ptrA	-8.02	0.226801	174 -- 191	5 -- 22	protease III		_____________173________________192\n_____________\|__________________\|\n5'-GCU...ACAGA____U________U____GGUCC...CCA-3'\n______________AAAU_AAGUUGAU_AUGA\n______________\|\|\|\|_\|\|\|\|\|:\|\|_\|\|\|\|\n______________UUUA_UUCAAUUA_UACU\n3'-UUU...CAUUA____U________C____AUCG-5'\n_____________\|__________________\|\n_____________23_________________4\n	180 -- 186	10 -- 16	-10.4485 kcal/mol	2.32937 kcal/mol	0.0977783 kcal/mol	173 192@LB@ \| \|@LB@5'-GCU...ACAGA U U GGUCC...CCA-3'@LB@ AAAU AAGUUGAU AUGA@LB@ \|\|\|\| \|\|\|\|\|:\|\| \|\|\|\|@LB@ UUUA UUCAAUUA UACU@LB@3'-UUU...CAUUA U C AUCG-5'@LB@ \| \|@LB@ 23 4@LB@@LB@@LB@Energy -8.02 kcal/mol@LB@Hybridization Energy -10.4485 kcal/mol@LB@Unfolding Energy - mRNA 2.32937 kcal/mol@LB@Unfolding Energy - sRNA 0.0977783 kcal/mol@LB@@LB@Position - mRNA 174 -- 191@LB@Position - sRNA 5 -- 22@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 10 -- 16@LB@
60	0.008489	0.4523	b3912	cpxR	-10.34	0.082729	218 -- 229	5 -- 16	response regulator in two-component regulatory system with CpxA		_____________217__________230\n_____________\|____________\|\n5'-UGA...CUGUU____________CCGAG...AUG-3'\n______________AGUUGAUGAUGA\n______________\|\|\|\|:\|\|\|\|\|\|\|\n______________UCAAUUACUACU\n3'-UUU...UUAUU____________AUCG-5'\n_____________\|____________\|\n_____________17___________4\n	223 -- 229	5 -- 11	-14.1725 kcal/mol	3.78244 kcal/mol	0.0462955 kcal/mol	217 230@LB@ \| \|@LB@5'-UGA...CUGUU CCGAG...AUG-3'@LB@ AGUUGAUGAUGA@LB@ \|\|\|\|:\|\|\|\|\|\|\|@LB@ UCAAUUACUACU@LB@3'-UUU...UUAUU AUCG-5'@LB@ \| \|@LB@ 17 4@LB@@LB@@LB@Energy -10.34 kcal/mol@LB@Hybridization Energy -14.1725 kcal/mol@LB@Unfolding Energy - mRNA 3.78244 kcal/mol@LB@Unfolding Energy - sRNA 0.0462955 kcal/mol@LB@@LB@Position - mRNA 218 -- 229@LB@Position - sRNA 5 -- 16@LB@Position Seed - mRNA 223 -- 229@LB@Position Seed - sRNA 5 -- 11@LB@
93	0.0151	0.5059	b1675	ydhZ	-8.81	0.164498	185 -- 226	5 -- 54	uncharacterized protein		_____________184________________________________________________227\n_____________\|__________________________________________________\|\n5'-CUU...CAUGA___________AA____UG___AAU___AC____________________ACUGU...AGC-3'\n______________UAAAUAGGUAU__AGAA__GGA___CGG____AAA_AGAG_____GAUGA\n______________\|\|\|\|:\|\|::\|\|__\|\|\|\|__\|:\|___\|\|\|____\|\|\|_\|:\|\|_____\|\|\|\|\|\n______________AUUUGUCUGUA__UCUU__CUU___GCC____UUU_UUUC_____CUACU\n3'-UUU...UCCCC___________AG____UA___ACU___AUUA___A____AAUUA_____AUCG-5'\n_____________\|__________________________________________________\|\n_____________55_________________________________________________4\n	186 -- 192	47 -- 53	-19.2855 kcal/mol	4.14282 kcal/mol	6.33746 kcal/mol	184 227@LB@ \| \|@LB@5'-CUU...CAUGA AA UG AAU AC ACUGU...AGC-3'@LB@ UAAAUAGGUAU AGAA GGA CGG AAA AGAG GAUGA@LB@ \|\|\|\|:\|\|::\|\| \|\|\|\| \|:\| \|\|\| \|\|\| \|:\|\| \|\|\|\|\|@LB@ AUUUGUCUGUA UCUU CUU GCC UUU UUUC CUACU@LB@3'-UUU...UCCCC AG UA ACU AUUA A AAUUA AUCG-5'@LB@ \| \|@LB@ 55 4@LB@@LB@@LB@Energy -8.81 kcal/mol@LB@Hybridization Energy -19.2855 kcal/mol@LB@Unfolding Energy - mRNA 4.14282 kcal/mol@LB@Unfolding Energy - sRNA 6.33746 kcal/mol@LB@@LB@Position - mRNA 185 -- 226@LB@Position - sRNA 5 -- 54@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 47 -- 53@LB@
97	0.01584	0.5059	b0116	lpd	-10.63	0.072174	157 -- 204	5 -- 49	dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase		_____________156_______________________________________________205\n_____________\|_________________________________________________\|\n5'-CCG...CUGAA_____A_____U____CC_C___A________UAUA___GUC_______GUACU...UAA-3'\n______________AGACG__CGGG_AUGA__G_CGG_GAUAAAUA____GAG___AUGAUGA\n______________\|\|\|\|:__\|:\|:_\|\|\|\|__\|_\|\|\|_:\|\|\|\|\|\|\|____:\|\|___\|\|\|\|\|\|\|\n______________UCUGU__GUCU_UACU__C_GCC_UUAUUUAU____UUC___UACUACU\n3'-UUU...AUUUG_____AA____U____UA_U___A_______________AAU_______AUCG-5'\n_____________\|_________________________________________________\|\n_____________50________________________________________________4\n	198 -- 204	5 -- 11	-27.6335 kcal/mol	10.6788 kcal/mol	6.32229 kcal/mol	156 205@LB@ \| \|@LB@5'-CCG...CUGAA A U CC C A UAUA GUC GUACU...UAA-3'@LB@ AGACG CGGG AUGA G CGG GAUAAAUA GAG AUGAUGA@LB@ \|\|\|\|: \|:\|: \|\|\|\| \| \|\|\| :\|\|\|\|\|\|\| :\|\| \|\|\|\|\|\|\|@LB@ UCUGU GUCU UACU C GCC UUAUUUAU UUC UACUACU@LB@3'-UUU...AUUUG AA U UA U A AAU AUCG-5'@LB@ \| \|@LB@ 50 4@LB@@LB@@LB@Energy -10.63 kcal/mol@LB@Hybridization Energy -27.6335 kcal/mol@LB@Unfolding Energy - mRNA 10.6788 kcal/mol@LB@Unfolding Energy - sRNA 6.32229 kcal/mol@LB@@LB@Position - mRNA 157 -- 204@LB@Position - sRNA 5 -- 49@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 5 -- 11@LB@
126	0.01995	0.5155	b2452	eutH	-9.16	0.141344	190 -- 206	5 -- 22	ethanolamine transporter		_____________189________________207\n_____________\|__________________\|\n5'-UUG...ACAGG________GC________AUUAA...UCC-3'\n______________GAGUAAAG__GAUGG_GA\n______________:\|:\|\|\|\|\|__:\|\|\|:_\|\|\n______________UUUAUUUC__UUACU_CU\n3'-UUU...CAUUA________AA_____A__AUCG-5'\n_____________\|__________________\|\n_____________23_________________4\n	191 -- 197	15 -- 21	-11.7395 kcal/mol	2.48279 kcal/mol	0.0977783 kcal/mol	189 207@LB@ \| \|@LB@5'-UUG...ACAGG GC AUUAA...UCC-3'@LB@ GAGUAAAG GAUGG GA@LB@ :\|:\|\|\|\|\| :\|\|\|: \|\|@LB@ UUUAUUUC UUACU CU@LB@3'-UUU...CAUUA AA A AUCG-5'@LB@ \| \|@LB@ 23 4@LB@@LB@@LB@Energy -9.16 kcal/mol@LB@Hybridization Energy -11.7395 kcal/mol@LB@Unfolding Energy - mRNA 2.48279 kcal/mol@LB@Unfolding Energy - sRNA 0.0977783 kcal/mol@LB@@LB@Position - mRNA 190 -- 206@LB@Position - sRNA 5 -- 22@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 15 -- 21@LB@
100	0.01591	0.5059	b0531	sfmC	-11.86	0.039421	211 -- 227	6 -- 22	putative periplasmic pilus chaperone	b4316	_____________210_______________228\n_____________\|_________________\|\n5'-AAU...GACUA___________U_____CUCAU...GUA-3'\n______________AAAUAAAGUUA_UGAUG\n______________\|\|\|\|\|\|\|\|\|\|\|_\|\|\|\|\|\n______________UUUAUUUCAAU_ACUAC\n3'-UUU...CAUUA___________U_____UAUCG-5'\n_____________\|_________________\|\n_____________23________________5\n	215 -- 221	12 -- 18	-15.888 kcal/mol	3.9302 kcal/mol	0.0976382 kcal/mol	210 228@LB@ \| \|@LB@5'-AAU...GACUA U CUCAU...GUA-3'@LB@ AAAUAAAGUUA UGAUG@LB@ \|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|@LB@ UUUAUUUCAAU ACUAC@LB@3'-UUU...CAUUA U UAUCG-5'@LB@ \| \|@LB@ 23 5@LB@@LB@@LB@Energy -11.86 kcal/mol@LB@Hybridization Energy -15.888 kcal/mol@LB@Unfolding Energy - mRNA 3.9302 kcal/mol@LB@Unfolding Energy - sRNA 0.0976382 kcal/mol@LB@@LB@Position - mRNA 211 -- 227@LB@Position - sRNA 6 -- 22@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 12 -- 18@LB@
176	0.02871	0.5353	b0518	fdrA	-11.39	0.049925	288 -- 297	6 -- 15	putative NAD(P)-binding acyl-CoA synthetase	b0320	_____________287________298\n_____________\|__________\|\n5'-CGC...AAAAU__________UUU-3'\n______________GUUGAUGAUG\n______________\|\|\|:\|\|\|\|\|\|\n______________CAAUUACUAC\n3'-UUU...UAUUU__________UAUCG-5'\n_____________\|__________\|\n_____________16_________5\n	291 -- 297	6 -- 12	-11.8608 kcal/mol	0.426728 kcal/mol	0.0442104 kcal/mol	287 298@LB@ \| \|@LB@5'-CGC...AAAAU UUU-3'@LB@ GUUGAUGAUG@LB@ \|\|\|:\|\|\|\|\|\|@LB@ CAAUUACUAC@LB@3'-UUU...UAUUU UAUCG-5'@LB@ \| \|@LB@ 16 5@LB@@LB@@LB@Energy -11.39 kcal/mol@LB@Hybridization Energy -11.8608 kcal/mol@LB@Unfolding Energy - mRNA 0.426728 kcal/mol@LB@Unfolding Energy - sRNA 0.0442104 kcal/mol@LB@@LB@Position - mRNA 288 -- 297@LB@Position - sRNA 6 -- 15@LB@Position Seed - mRNA 291 -- 297@LB@Position Seed - sRNA 6 -- 12@LB@
23	0.001279	0.1825	b0199	metN	-18.57	0.000714	152 -- 205	7 -- 50	DL-methionine transporter subunit		_____________151_______________________________________________________206\n_____________\|_________________________________________________________\|\n5'-CGC...CGUUU________________CCACGACUAACUU_______A__________UC________AAAAC...UUU-3'\n______________CAGGCAUUC_GAGAUG_____________AAUGACG_UAAUAAAUAA____AAUGAU\n______________\|\|\|:\|\|\|\|\|_\|\|:\|\|\|_____________\|\|\|\|\|\|\|_\|\|\|\|\|\|\|\|\|\|____\|\|\|\|\|\|\n______________GUCUGUAAG_CUUUAC_____________UUACUGC_AUUAUUUAUU____UUACUA\n3'-UUU...CAUUU_________U__________________________C__________UCAA______CUAUCG-5'\n_____________\|_________________________________________________________\|\n_____________51________________________________________________________6\n	191 -- 197	17 -- 23	-35.1892 kcal/mol	10.2873 kcal/mol	6.33206 kcal/mol	151 206@LB@ \| \|@LB@5'-CGC...CGUUU CCACGACUAACUU A UC AAAAC...UUU-3'@LB@ CAGGCAUUC GAGAUG AAUGACG UAAUAAAUAA AAUGAU@LB@ \|\|\|:\|\|\|\|\| \|\|:\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|@LB@ GUCUGUAAG CUUUAC UUACUGC AUUAUUUAUU UUACUA@LB@3'-UUU...CAUUU U C UCAA CUAUCG-5'@LB@ \| \|@LB@ 51 6@LB@@LB@@LB@Energy -18.57 kcal/mol@LB@Hybridization Energy -35.1892 kcal/mol@LB@Unfolding Energy - mRNA 10.2873 kcal/mol@LB@Unfolding Energy - sRNA 6.33206 kcal/mol@LB@@LB@Position - mRNA 152 -- 205@LB@Position - sRNA 7 -- 50@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 17 -- 23@LB@
74	0.0107	0.4523	b3385	gph	-12.85	0.023515	41 -- 77	8 -- 48	phosphoglycolate phosphatase		_____________40____________________________________________78\n_____________\|_____________________________________________\|\n5'-ACU...GCUUU_______GACU__________________C_______________ACAAC...UGU-3'\n______________GACAUUC____AGAAGUG___GACGGUGG___GUGAAG___GUGA\n______________\|\|\|\|\|\|\|____\|\|\|\|:\|\|___\|\|\|\|\|\|::___:\|:\|\|\|___:\|\|\|\n______________CUGUAAG____UCUUUAC___CUGCCAUU___UAUUUC___UACU\n3'-UUU...UUUGU__________________UUA________AUU______AAU____ACUAUCG-5'\n_____________\|_____________________________________________\|\n_____________49____________________________________________7\n	60 -- 66	24 -- 30	-29.3756 kcal/mol	10.2075 kcal/mol	6.31567 kcal/mol	40 78@LB@ \| \|@LB@5'-ACU...GCUUU GACU C ACAAC...UGU-3'@LB@ GACAUUC AGAAGUG GACGGUGG GUGAAG GUGA@LB@ \|\|\|\|\|\|\| \|\|\|\|:\|\| \|\|\|\|\|\|:: :\|:\|\|\| :\|\|\|@LB@ CUGUAAG UCUUUAC CUGCCAUU UAUUUC UACU@LB@3'-UUU...UUUGU UUA AUU AAU ACUAUCG-5'@LB@ \| \|@LB@ 49 7@LB@@LB@@LB@Energy -12.85 kcal/mol@LB@Hybridization Energy -29.3756 kcal/mol@LB@Unfolding Energy - mRNA 10.2075 kcal/mol@LB@Unfolding Energy - sRNA 6.31567 kcal/mol@LB@@LB@Position - mRNA 41 -- 77@LB@Position - sRNA 8 -- 48@LB@Position Seed - mRNA 60 -- 66@LB@Position Seed - sRNA 24 -- 30@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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