Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
157	0.01768	0.3749	b1216	chaA	-11.60	0.134178	242 -- 292	18 -- 65	calcium/sodium:proton antiporter	_____________241_______________293\n_____________\|_________________\|\n5'-AUU...AAUGU_________C___A___UUUCU...GCC-3'\n______________UACCGAUAU_CAG_CAC\n______________:\|\|\|:\|\|\|:_\|\|\|_\|\|\|\n______________GUGGUUAUG_GUC_GUG\n3'-UUU...AAAAU_________A___A___UGUAC...UAG-5'\n_____________\|_________________\|\n_____________66________________17\n	120 -- 126	178 -- 184	-16.2011 kcal/mol	3.68451 kcal/mol	4.86651 kcal/mol	241 293@LB@ \| \|@LB@5'-AUU...AAUGU C A UUUCU...GCC-3'@LB@ UACCGAUAU CAG CAC@LB@ :\|\|\|:\|\|\|: \|\|\| \|\|\|@LB@ GUGGUUAUG GUC GUG@LB@3'-UUU...AAAAU A A UGUAC...UAG-5'@LB@ \| \|@LB@ 66 17@LB@@LB@@LB@Energy -11.60 kcal/mol@LB@Hybridization Energy -16.2011 kcal/mol@LB@Unfolding Energy - mRNA 3.68451 kcal/mol@LB@Unfolding Energy - sRNA 4.86651 kcal/mol@LB@@LB@Position - mRNA 242 -- 292@LB@Position - sRNA 18 -- 65@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 178 -- 184@LB@
195	0.02319	0.3833	b1233	ychJ	-7.86	0.620262	61 -- 80	1 -- 18	UPF0225 family protein	_____________60____________________________________81\n_____________\|_____________________________________\|\n5'-CUU...GCUAC_______________A___CUACU_____________ACGCC...UCA-3'\n______________UGCUCGGUUA_ACAU_GUG_____CAGGC_GGUUAAU\n______________\|:\|\|\|:\|\|:\|_\|\|\|\|_:\|\|_____\|\|\|\|\|_:\|\|:\|\|:\n______________AUGAGUCAGU_UGUA_UAC_____GUCCG_UCAGUUG\n3'-UUU...UGGUU__________G____C_____________U_______AAAGU...UAG-5'\n_____________\|_____________________________________\|\n_____________19____________________________________0\n	139 -- 145	171 -- 177	-29.0991 kcal/mol	9.80481 kcal/mol	8.18679 kcal/mol	60 81@LB@ \| \|@LB@5'-CUU...GCUAC A CUACU ACGCC...UCA-3'@LB@ UGCUCGGUUA ACAU GUG CAGGC GGUUAAU@LB@ \|:\|\|\|:\|\|:\| \|\|\|\| :\|\| \|\|\|\|\| :\|\|:\|\|:@LB@ AUGAGUCAGU UGUA UAC GUCCG UCAGUUG@LB@3'-UUU...UGGUU G C U AAAGU...UAG-5'@LB@ \| \|@LB@ 19 0@LB@@LB@@LB@Energy -7.86 kcal/mol@LB@Hybridization Energy -29.0991 kcal/mol@LB@Unfolding Energy - mRNA 9.80481 kcal/mol@LB@Unfolding Energy - sRNA 8.18679 kcal/mol@LB@@LB@Position - mRNA 61 -- 80@LB@Position - sRNA 1 -- 18@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 171 -- 177@LB@
74	0.007724	0.3458	b1261	trpB	-16.40	0.014006	36 -- 47	195 -- 206	tryptophan synthase beta subunit	_____________35___________48\n_____________\|____________\|\n5'-UGG...AACGU____________GGGCU...UCA-3'\n______________UCUGCUGGCGGG\n______________\|\|\|\|\|\|\|\|\|\|\|\|\n______________AGACGACCGCCC\n3'-UUU...AUAUU____________ACUAA...UAG-5'\n_____________\|____________\|\n_____________207__________194\n	41 -- 47	195 -- 201	-26.9412 kcal/mol	6.0368 kcal/mol	9.05854 kcal/mol	35 48@LB@ \| \|@LB@5'-UGG...AACGU GGGCU...UCA-3'@LB@ UCUGCUGGCGGG@LB@ \|\|\|\|\|\|\|\|\|\|\|\|@LB@ AGACGACCGCCC@LB@3'-UUU...AUAUU ACUAA...UAG-5'@LB@ \| \|@LB@ 207 194@LB@@LB@@LB@Energy -16.40 kcal/mol@LB@Hybridization Energy -26.9412 kcal/mol@LB@Unfolding Energy - mRNA 6.0368 kcal/mol@LB@Unfolding Energy - sRNA 9.05854 kcal/mol@LB@@LB@Position - mRNA 36 -- 47@LB@Position - sRNA 195 -- 206@LB@Position Seed - mRNA 41 -- 47@LB@Position Seed - sRNA 195 -- 201@LB@
49	0.004667	0.3205	b1277	ribA	-9.86	0.291903	241 -- 249	16 -- 24	GTP cyclohydrolase II	_____________240________250\n_____________\|__________\|\n5'-CCA...AAUAC__________UUAUC...UCG-3'\n______________AGAACCAGCA\n______________\|:\|\|\|\|\|\|\|\|\n______________UUUUGGUCGU\n_______3'-UUUU__________CCAUA...UAG-5'\n_____________\|__________\|\n_____________25_________15\n	178 -- 184	214 -- 220	-13.9783 kcal/mol	2.87719 kcal/mol	5.75438 kcal/mol	240 250@LB@ \| \|@LB@5'-CCA...AAUAC UUAUC...UCG-3'@LB@ AGAACCAGCA@LB@ \|:\|\|\|\|\|\|\|\|@LB@ UUUUGGUCGU@LB@ 3'-UUUU CCAUA...UAG-5'@LB@ \| \|@LB@ 25 15@LB@@LB@@LB@Energy -9.86 kcal/mol@LB@Hybridization Energy -13.9783 kcal/mol@LB@Unfolding Energy - mRNA 2.87719 kcal/mol@LB@Unfolding Energy - sRNA 5.75438 kcal/mol@LB@@LB@Position - mRNA 241 -- 249@LB@Position - sRNA 16 -- 24@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 214 -- 220@LB@
39	0.003509	0.2912	b1293	sapB	-10.75	0.197427	9 -- 16	196 -- 203	antimicrobial peptide transport ABC transporter permease	_____________8____________17\n_____________\|____________\|\n5'-UCG...UAUUU_______CA___GC-3'\n______________CACCCCU__UGC\n______________\|\|\|\|\|\|:__\|\|\|\n______________GUGGGGG__ACG\n3'-UUU...ACCCC_______A____AAGUAG-5'\n_____________\|____________\|\n_____________204__________195\n	287 -- 293	11 -- 17	-15.5024 kcal/mol	0.267702 kcal/mol	6.55606 kcal/mol	8 17@LB@ \| \|@LB@5'-UCG...UAUUU CA GC-3'@LB@ CACCCCU UGC@LB@ \|\|\|\|\|\|: \|\|\|@LB@ GUGGGGG ACG@LB@3'-UUU...ACCCC A AAGUAG-5'@LB@ \| \|@LB@ 204 195@LB@@LB@@LB@Energy -10.75 kcal/mol@LB@Hybridization Energy -15.5024 kcal/mol@LB@Unfolding Energy - mRNA 0.267702 kcal/mol@LB@Unfolding Energy - sRNA 6.55606 kcal/mol@LB@@LB@Position - mRNA 9 -- 16@LB@Position - sRNA 196 -- 203@LB@Position Seed - mRNA 287 -- 293@LB@Position Seed - sRNA 11 -- 17@LB@
110	0.01217	0.3575	b1324	tpx	-10.54	0.217228	251 -- 258	126 -- 133	lipid hydroperoxide peroxidase	_____________250____________259\n_____________\|______________\|\n5'-GCG...ACUCC______________GUCUG...AAG-3'\n______________UUGUUGUCGCGGUG\n______________\|:\|:\|\|\|\|\|\|\|\|\|:\n______________AGCGACAGCGCCAU\n3'-UUU...GUUGA______________UUUAU...UAG-5'\n_____________\|______________\|\n_____________134____________125\n	99 -- 105	48 -- 54	-22.761 kcal/mol	7.03952 kcal/mol	5.88061 kcal/mol	250 259@LB@ \| \|@LB@5'-GCG...ACUCC GUCUG...AAG-3'@LB@ UUGUUGUCGCGGUG@LB@ \|:\|:\|\|\|\|\|\|\|\|\|:@LB@ AGCGACAGCGCCAU@LB@3'-UUU...GUUGA UUUAU...UAG-5'@LB@ \| \|@LB@ 134 125@LB@@LB@@LB@Energy -10.54 kcal/mol@LB@Hybridization Energy -22.761 kcal/mol@LB@Unfolding Energy - mRNA 7.03952 kcal/mol@LB@Unfolding Energy - sRNA 5.88061 kcal/mol@LB@@LB@Position - mRNA 251 -- 258@LB@Position - sRNA 126 -- 133@LB@Position Seed - mRNA 99 -- 105@LB@Position Seed - sRNA 48 -- 54@LB@
112	0.01232	0.3575	b1540	rspR	-15.19	0.024761	13 -- 44	171 -- 202	transcriptional repressor for rspAB	_____________12_________________________________________________________45\n_____________\|__________________________________________________________\|\n5'-CAG...GCCGU__________UCA_______GCC______C___A____CAAUACCCUGGAA_______CGGUU...GCA-3'\n______________CUGGUGGGUG_____ACGCU___UGCUCA_GUC_AUAU_____________AUGAUGC\n______________\|\|\|\|:\|\|\|\|\|_____\|\|:\|:___\|:\|\|\|\|_\|\|\|_\|:\|:_____________\|\|\|\|\|\|\|\n______________GACCGCCCAC_____UGUGG___AUGAGU_CAG_UGUG_____________UACUACG\n3'-UUU...UAGAC__________UAAAA_____UU____________________________________UCCGU...UAG-5'\n_____________\|__________________________________________________________\|\n_____________203________________________________________________________170\n	62 -- 68	161 -- 167	-40.6001 kcal/mol	15.8189 kcal/mol	17.3482 kcal/mol	12 45@LB@ \| \|@LB@5'-CAG...GCCGU UCA GCC C A CAAUACCCUGGAA CGGUU...GCA-3'@LB@ CUGGUGGGUG ACGCU UGCUCA GUC AUAU AUGAUGC@LB@ \|\|\|\|:\|\|\|\|\| \|\|:\|: \|:\|\|\|\| \|\|\| \|:\|: \|\|\|\|\|\|\|@LB@ GACCGCCCAC UGUGG AUGAGU CAG UGUG UACUACG@LB@3'-UUU...UAGAC UAAAA UU UCCGU...UAG-5'@LB@ \| \|@LB@ 203 170@LB@@LB@@LB@Energy -15.19 kcal/mol@LB@Hybridization Energy -40.6001 kcal/mol@LB@Unfolding Energy - mRNA 15.8189 kcal/mol@LB@Unfolding Energy - sRNA 17.3482 kcal/mol@LB@@LB@Position - mRNA 13 -- 44@LB@Position - sRNA 171 -- 202@LB@Position Seed - mRNA 62 -- 68@LB@Position Seed - sRNA 161 -- 167@LB@
118	0.01293	0.3575	b1598	ydgD	-17.08	0.010207	53 -- 140	115 -- 186	putative peptidase	_____________52______________________________________________________________________141\n_____________\|_______________________________________________________________________\|\n5'-CUU...AUCCU_____UAUG__________UACC__CC___UUUAU_____CU______A____UCUGAUUUUAC_______UUCAU...AGG-3'\n______________UAUUU____CGCAC_UGAU____GC__GUU_____CUUUC__GAUUGU_GUUA___________UCCCACU\n______________\|\|:\|:____\|:\|\|\|_\|\|\|\|____\|\|__\|\|:_____\|\|\|\|\|__:\|\|\|\|:_\|\|:\|___________\|\|\|\|\|\|\|\n______________AUGAG____GUGUG_ACUA____CG__CAG_____GAAAG__UUAACG_CAGU___________AGGGUGA\n3'-UUU...UGGUU_____UCA______U____CGUC__UU___UU________UC______C______________________CGUAG...UAG-5'\n_____________\|_______________________________________________________________________\|\n_____________187_____________________________________________________________________114\n	122 -- 128	125 -- 131	-34.2678 kcal/mol	6.55961 kcal/mol	16.6417 kcal/mol	52 141@LB@ \| \|@LB@5'-CUU...AUCCU UAUG UACC CC UUUAU CU A UCUGAUUUUAC UUCAU...AGG-3'@LB@ UAUUU CGCAC UGAU GC GUU CUUUC GAUUGU GUUA UCCCACU@LB@ \|\|:\|: \|:\|\|\| \|\|\|\| \|\| \|\|: \|\|\|\|\| :\|\|\|\|: \|\|:\| \|\|\|\|\|\|\|@LB@ AUGAG GUGUG ACUA CG CAG GAAAG UUAACG CAGU AGGGUGA@LB@3'-UUU...UGGUU UCA U CGUC UU UU UC C CGUAG...UAG-5'@LB@ \| \|@LB@ 187 114@LB@@LB@@LB@Energy -17.08 kcal/mol@LB@Hybridization Energy -34.2678 kcal/mol@LB@Unfolding Energy - mRNA 6.55961 kcal/mol@LB@Unfolding Energy - sRNA 16.6417 kcal/mol@LB@@LB@Position - mRNA 53 -- 140@LB@Position - sRNA 115 -- 186@LB@Position Seed - mRNA 122 -- 128@LB@Position Seed - sRNA 125 -- 131@LB@
143	0.01531	0.3575	b1641	slyB	-10.83	0.190300	158 -- 167	83 -- 92	outer membrane lipoprotein	_____________157____________________________168\n_____________\|______________________________\|\n5'-CCG...UCACA_______U______UGAC____AGG_____UUGCG...AAG-3'\n______________UAUGCCA_AUACUU____CAUG___GAUGC\n______________\|\|::\|\|\|_\|\|\|\|\|:____\|\|::___\|\|\|\|\|\n______________AUGUGGU_UAUGAG____GUGU___CUACG\n3'-UUU...CUAAA______________UCA_____GUA_____UCCGU...UAG-5'\n_____________\|______________________________\|\n_____________93_____________________________82\n	135 -- 141	182 -- 188	-20.8318 kcal/mol	7.22081 kcal/mol	7.72227 kcal/mol	157 168@LB@ \| \|@LB@5'-CCG...UCACA U UGAC AGG UUGCG...AAG-3'@LB@ UAUGCCA AUACUU CAUG GAUGC@LB@ \|\|::\|\|\| \|\|\|\|\|: \|\|:: \|\|\|\|\|@LB@ AUGUGGU UAUGAG GUGU CUACG@LB@3'-UUU...CUAAA UCA GUA UCCGU...UAG-5'@LB@ \| \|@LB@ 93 82@LB@@LB@@LB@Energy -10.83 kcal/mol@LB@Hybridization Energy -20.8318 kcal/mol@LB@Unfolding Energy - mRNA 7.22081 kcal/mol@LB@Unfolding Energy - sRNA 7.72227 kcal/mol@LB@@LB@Position - mRNA 158 -- 167@LB@Position - sRNA 83 -- 92@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 182 -- 188@LB@
191	0.02237	0.3833	b1658	purR	-11.05	0.172432	254 -- 265	163 -- 174	transcriptional repressor hypoxanthine-binding	_____________253_______________266\n_____________\|_________________\|\n5'-UUU...CUACA___GU____________CAACA...GCA-3'\n______________ACU__GUCACACGUGAU\n______________\|\|\|__\|\|\|\|\|\|\|:\|\|\|\|\n______________UGA__CAGUGUGUACUA\n3'-UUU...GGUUA___GU____________CGUCC...UAG-5'\n_____________\|_________________\|\n_____________175_______________162\n	259 -- 265	163 -- 169	-20.3148 kcal/mol	3.57431 kcal/mol	4.30742 kcal/mol	253 266@LB@ \| \|@LB@5'-UUU...CUACA GU CAACA...GCA-3'@LB@ ACU GUCACACGUGAU@LB@ \|\|\| \|\|\|\|\|\|\|:\|\|\|\|@LB@ UGA CAGUGUGUACUA@LB@3'-UUU...GGUUA GU CGUCC...UAG-5'@LB@ \| \|@LB@ 175 162@LB@@LB@@LB@Energy -11.05 kcal/mol@LB@Hybridization Energy -20.3148 kcal/mol@LB@Unfolding Energy - mRNA 3.57431 kcal/mol@LB@Unfolding Energy - sRNA 4.30742 kcal/mol@LB@@LB@Position - mRNA 254 -- 265@LB@Position - sRNA 163 -- 174@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 163 -- 169@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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