Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
62	0.006126	0.3261	b3824	rhtB	-10.68	0.204362	7 -- 23	171 -- 187	homoserine homoserine lactone and S-methyl-methionine efflux pump	_____________6_________________24\n_____________\|_________________\|\n_____5'-ACGCUG_______AAA_______GCGGU...ACC-3'\n______________ACGCCAG___UCAGUCA\n______________\|\|:\|\|\|:___\|\|\|\|\|\|\|\n______________UGUGGUU___AGUCAGU\n3'-UUU...UAAAA_______AUG_______GUGUA...UAG-5'\n_____________\|_________________\|\n_____________188_______________170\n	17 -- 23	171 -- 177	-18.1223 kcal/mol	1.9449 kcal/mol	4.7016 kcal/mol	6 24@LB@ \| \|@LB@ 5'-ACGCUG AAA GCGGU...ACC-3'@LB@ ACGCCAG UCAGUCA@LB@ \|\|:\|\|\|: \|\|\|\|\|\|\|@LB@ UGUGGUU AGUCAGU@LB@3'-UUU...UAAAA AUG GUGUA...UAG-5'@LB@ \| \|@LB@ 188 170@LB@@LB@@LB@Energy -10.68 kcal/mol@LB@Hybridization Energy -18.1223 kcal/mol@LB@Unfolding Energy - mRNA 1.9449 kcal/mol@LB@Unfolding Energy - sRNA 4.7016 kcal/mol@LB@@LB@Position - mRNA 7 -- 23@LB@Position - sRNA 171 -- 187@LB@Position Seed - mRNA 17 -- 23@LB@Position Seed - sRNA 171 -- 177@LB@
127	0.01385	0.3575	b3832	rmuC	-11.83	0.120260	236 -- 246	193 -- 203	DNA recombination protein	_____________235____________________________________________________________247\n_____________\|______________________________________________________________\|\n5'-AAA...UUGUA________AUUC___UUU_____________GUU__G______AGGC_______AUU_____AUCAU...AGC-3'\n______________CAG_CACA____UAU___GUGCGGGUAAGUU___GC__UCAGG____GUUGUGG___UCUCA\n______________\|\|\|_\|\|\|\|____:\|\|___:\|\|\|:\|\|:\|\|\|\|:___:\|__\|\|\|\|:____:\|\|\|:\|\|___\|\|:\|\|\n______________GUC_GUGU____GUA___UACGUCCGUUCAG___UG__AGUCU____UAACGCC___AGGGU\n3'-UUU...UAUGA___A___________C_______________U____AA________________AGU_____GACGU...UAG-5'\n_____________\|______________________________________________________________\|\n_____________204____________________________________________________________192\n	198 -- 204	135 -- 141	-42.9883 kcal/mol	19.1075 kcal/mol	15.6146 kcal/mol	235 247@LB@ \| \|@LB@5'-AAA...UUGUA AUUC UUU GUU G AGGC AUU AUCAU...AGC-3'@LB@ CAG CACA UAU GUGCGGGUAAGUU GC UCAGG GUUGUGG UCUCA@LB@ \|\|\| \|\|\|\| :\|\| :\|\|\|:\|\|:\|\|\|\|: :\| \|\|\|\|: :\|\|\|:\|\| \|\|:\|\|@LB@ GUC GUGU GUA UACGUCCGUUCAG UG AGUCU UAACGCC AGGGU@LB@3'-UUU...UAUGA A C U AA AGU GACGU...UAG-5'@LB@ \| \|@LB@ 204 192@LB@@LB@@LB@Energy -11.83 kcal/mol@LB@Hybridization Energy -42.9883 kcal/mol@LB@Unfolding Energy - mRNA 19.1075 kcal/mol@LB@Unfolding Energy - sRNA 15.6146 kcal/mol@LB@@LB@Position - mRNA 236 -- 246@LB@Position - sRNA 193 -- 203@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 135 -- 141@LB@
65	0.006332	0.3261	b3857	mobA	-11.59	0.134663	143 -- 181	126 -- 173	molybdopterin-guanine dinucleotide synthase	_____________142________________________________________________________182\n_____________\|__________________________________________________________\|\n5'-UCC...AUAGU_______C_____U_U___GA___A__C______AG______________A_______AAUAA...AAC-3'\n______________GAUGCUC__UUAC_C_UGA__CAG_CA_GUUAGC____________GGUC_AUCCCAC\n______________:\|\|:\|\|\|__\|:\|\|_\|_\|\|\|__\|\|\|_\|\|_\|\|:\|:\|____________\|\|\|\|_\|\|\|\|\|\|\|\n______________UUAUGAG__AGUG_G_ACU__GUC_GU_CAGUUG____________CCAG_UAGGGUG\n3'-UUU...UGUGG_______UC____U_U___AC___C__U______AAAGUCUUAACG____________ACGUA...UAG-5'\n_____________\|__________________________________________________________\|\n_____________174________________________________________________________125\n	175 -- 181	126 -- 132	-38.7055 kcal/mol	10.57 kcal/mol	15.6637 kcal/mol	142 182@LB@ \| \|@LB@5'-UCC...AUAGU C U U GA A C AG A AAUAA...AAC-3'@LB@ GAUGCUC UUAC C UGA CAG CA GUUAGC GGUC AUCCCAC@LB@ :\|\|:\|\|\| \|:\|\| \| \|\|\| \|\|\| \|\| \|\|:\|:\| \|\|\|\| \|\|\|\|\|\|\|@LB@ UUAUGAG AGUG G ACU GUC GU CAGUUG CCAG UAGGGUG@LB@3'-UUU...UGUGG UC U U AC C U AAAGUCUUAACG ACGUA...UAG-5'@LB@ \| \|@LB@ 174 125@LB@@LB@@LB@Energy -11.59 kcal/mol@LB@Hybridization Energy -38.7055 kcal/mol@LB@Unfolding Energy - mRNA 10.57 kcal/mol@LB@Unfolding Energy - sRNA 15.6637 kcal/mol@LB@@LB@Position - mRNA 143 -- 181@LB@Position - sRNA 126 -- 173@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 126 -- 132@LB@
107	0.01169	0.3575	b3858	yihD	-12.50	0.087903	89 -- 104	63 -- 77	DUF1040 protein YihD	_____________88______________________________105\n_____________\|_______________________________\|\n5'-AUA...UUUAC___CU___________GCC____AUC_____CAUCA...CGA-3'\n______________CGC__GCCAGCACAAC___UGUU___GUCGU\n______________\|\|\|__:\|\|\|\|\|\|\|\|\|\|___:\|:\|___\|\|\|\|:\n______________GCG__UGGUCGUGUUG___GCGA___CAGCG\n3'-UUU...UGGUU___U____________AA_____________CCAUU...UAG-5'\n_____________\|_______________________________\|\n_____________78______________________________62\n	98 -- 104	63 -- 69	-31.4348 kcal/mol	4.67185 kcal/mol	17.7932 kcal/mol	88 105@LB@ \| \|@LB@5'-AUA...UUUAC CU GCC AUC CAUCA...CGA-3'@LB@ CGC GCCAGCACAAC UGUU GUCGU@LB@ \|\|\| :\|\|\|\|\|\|\|\|\|\| :\|:\| \|\|\|\|:@LB@ GCG UGGUCGUGUUG GCGA CAGCG@LB@3'-UUU...UGGUU U AA CCAUU...UAG-5'@LB@ \| \|@LB@ 78 62@LB@@LB@@LB@Energy -12.50 kcal/mol@LB@Hybridization Energy -31.4348 kcal/mol@LB@Unfolding Energy - mRNA 4.67185 kcal/mol@LB@Unfolding Energy - sRNA 17.7932 kcal/mol@LB@@LB@Position - mRNA 89 -- 104@LB@Position - sRNA 63 -- 77@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 63 -- 69@LB@
35	0.002845	0.2736	b3887	dtd	-14.98	0.027287	55 -- 74	191 -- 211	D-tyr-tRNA(Tyr) deacylase	_____________54____________________75\n_____________\|_____________________\|\n5'-GGA...UCAUU______G______________CCCCA...UCG-3'\n______________UAUGGU_UGCUGGCGG_UGAU\n______________\|\|\|::\|_\|\|\|\|\|\|\|\|\|_\|\|\|\|\n______________AUAUUA_ACGACCGCC_ACUA\n3'-UUU...CGUCC______G_________C____AAAUG...UAG-5'\n_____________\|_____________________\|\n_____________212___________________190\n	64 -- 70	196 -- 202	-27.702 kcal/mol	6.2401 kcal/mol	11.5765 kcal/mol	54 75@LB@ \| \|@LB@5'-GGA...UCAUU G CCCCA...UCG-3'@LB@ UAUGGU UGCUGGCGG UGAU@LB@ \|\|\|::\| \|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ AUAUUA ACGACCGCC ACUA@LB@3'-UUU...CGUCC G C AAAUG...UAG-5'@LB@ \| \|@LB@ 212 190@LB@@LB@@LB@Energy -14.98 kcal/mol@LB@Hybridization Energy -27.702 kcal/mol@LB@Unfolding Energy - mRNA 6.2401 kcal/mol@LB@Unfolding Energy - sRNA 11.5765 kcal/mol@LB@@LB@Position - mRNA 55 -- 74@LB@Position - sRNA 191 -- 211@LB@Position Seed - mRNA 64 -- 70@LB@Position Seed - sRNA 196 -- 202@LB@
3	8.668e-05	0.08442	b3892	fdoI	-15.80	0.018551	255 -- 278	1 -- 22	formate dehydrogenase-O cytochrome b556 subunit	_____________254_________________279\n_____________\|___________________\|\n5'-CCA...UGGAU____G___UC_________CUGGC...UGG-3'\n______________CACC_CCU__UGCUUCAUC\n______________\|\|\|\|_\|\|\|__\|\|\|\|\|\|\|\|\|\n______________GUGG_GGA__ACGAAGUAG\n3'-UUU...ACCCC____G______________-5'\n_____________\|__________________\|\n_____________23_________________1\n	272 -- 278	1 -- 7	-24.2792 kcal/mol	4.44512 kcal/mol	7.16879 kcal/mol	254 279@LB@ \| \|@LB@5'-CCA...UGGAU G UC CUGGC...UGG-3'@LB@ CACC CCU UGCUUCAUC@LB@ \|\|\|\| \|\|\| \|\|\|\|\|\|\|\|\|@LB@ GUGG GGA ACGAAGUAG@LB@3'-UUU...ACCCC G -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -15.80 kcal/mol@LB@Hybridization Energy -24.2792 kcal/mol@LB@Unfolding Energy - mRNA 4.44512 kcal/mol@LB@Unfolding Energy - sRNA 7.16879 kcal/mol@LB@@LB@Position - mRNA 255 -- 278@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 272 -- 278@LB@Position Seed - sRNA 1 -- 7@LB@
170	0.01941	0.3812	b3911	cpxA	-13.13	0.065341	265 -- 299	168 -- 204	sensory histidine kinase in two-component regulatory system with CpxR	_____________264______300\n_____________\|________\|\n5'-UAU...GUUUC________GAUAG...GCC-3'\n______________UGCUUCAU\n______________\|\|\|\|\|\|\|\|\n______________ACGAAGUA\n3'-UUU...GGGGA________G-5'\n_____________\|________\|\n_____________205______167\n	196 -- 202	2 -- 8	-10.3801 kcal/mol	3.0426 kcal/mol	0.709873 kcal/mol	264 300@LB@ \| \|@LB@5'-UAU...GUUUC GAUAG...GCC-3'@LB@ UGCUUCAU@LB@ \|\|\|\|\|\|\|\|@LB@ ACGAAGUA@LB@3'-UUU...GGGGA G-5'@LB@ \| \|@LB@ 205 167@LB@@LB@@LB@Energy -13.13 kcal/mol@LB@Hybridization Energy -10.3801 kcal/mol@LB@Unfolding Energy - mRNA 3.0426 kcal/mol@LB@Unfolding Energy - sRNA 0.709873 kcal/mol@LB@@LB@Position - mRNA 265 -- 299@LB@Position - sRNA 168 -- 204@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 2 -- 8@LB@
160	0.01787	0.3749	b3935	priA	-8.27	0.542824	222 -- 250	110 -- 140	Primosome factor n' (replication factor Y)	_____________221_________________________251\n_____________\|___________________________\|\n5'-GAA...UAACC______AU___A_____G_________UAUGC...GCG-3'\n______________CACCGA__UUC_AGUCA_G_AUGAUGC\n______________\|\|\|\|:\|__:\|\|_\|\|\|\|\|_:_\|\|\|\|\|\|\|\n______________GUGGUU__GAG_UCAGU_U_UACUACG\n3'-UUU...AAAAU______AU_________G_G_______UCCGU...UAG-5'\n_____________\|___________________________\|\n_____________141_________________________109\n	193 -- 199	161 -- 167	-24.457 kcal/mol	4.47787 kcal/mol	7.72013 kcal/mol	221 251@LB@ \| \|@LB@5'-GAA...UAACC AU A G UAUGC...GCG-3'@LB@ CACCGA UUC AGUCA G AUGAUGC@LB@ \|\|\|\|:\| :\|\| \|\|\|\|\| : \|\|\|\|\|\|\|@LB@ GUGGUU GAG UCAGU U UACUACG@LB@3'-UUU...AAAAU AU G G UCCGU...UAG-5'@LB@ \| \|@LB@ 141 109@LB@@LB@@LB@Energy -8.27 kcal/mol@LB@Hybridization Energy -24.457 kcal/mol@LB@Unfolding Energy - mRNA 4.47787 kcal/mol@LB@Unfolding Energy - sRNA 7.72013 kcal/mol@LB@@LB@Position - mRNA 222 -- 250@LB@Position - sRNA 110 -- 140@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 161 -- 167@LB@
137	0.01486	0.3575	b3967	murI	-16.98	0.010727	107 -- 167	42 -- 96	glutamate racemase	_____________106__________________________168\n_____________\|____________________________\|\n5'-GUU...GACUG________ACU_____________G___UCGCU...GGU-3'\n______________GGGU_AUU___UAUCAGUAUUUAG_CAC\n______________\|\|\|\|_\|\|\|___:\|:\|\|:\|\|:\|:\|\|_\|\|\|\n______________CCCA_UAA___GUGGUUAUGAGUC_GUG\n3'-UUU...GACCG____C___AAU_____________A___UGUAC...UAG-5'\n_____________\|____________________________\|\n_____________97___________________________41\n	35 -- 41	174 -- 180	-22.1424 kcal/mol	5.45848 kcal/mol	5.03792 kcal/mol	106 168@LB@ \| \|@LB@5'-GUU...GACUG ACU G UCGCU...GGU-3'@LB@ GGGU AUU UAUCAGUAUUUAG CAC@LB@ \|\|\|\| \|\|\| :\|:\|\|:\|\|:\|:\|\| \|\|\|@LB@ CCCA UAA GUGGUUAUGAGUC GUG@LB@3'-UUU...GACCG C AAU A UGUAC...UAG-5'@LB@ \| \|@LB@ 97 41@LB@@LB@@LB@Energy -16.98 kcal/mol@LB@Hybridization Energy -22.1424 kcal/mol@LB@Unfolding Energy - mRNA 5.45848 kcal/mol@LB@Unfolding Energy - sRNA 5.03792 kcal/mol@LB@@LB@Position - mRNA 107 -- 167@LB@Position - sRNA 42 -- 96@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 174 -- 180@LB@
90	0.01008	0.3575	b3994	thiC	-11.53	0.138483	145 -- 158	1 -- 14	phosphomethylpyrimidine synthase	_____________144_________159\n_____________\|___________\|\n5'-AUG...CGUCU___________CGUCG...UUU-3'\n______________CUUGCUUCAUC\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________GAACGAAGUAG\n3'-UUU...UGGGG___________-5'\n_____________\|__________\|\n_____________15_________1\n	152 -- 158	1 -- 7	-17.5294 kcal/mol	1.22878 kcal/mol	1.03329 kcal/mol	144 159@LB@ \| \|@LB@5'-AUG...CGUCU CGUCG...UUU-3'@LB@ CUUGCUUCAUC@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ GAACGAAGUAG@LB@3'-UUU...UGGGG -5'@LB@ \| \|@LB@ 15 1@LB@@LB@@LB@Energy -11.53 kcal/mol@LB@Hybridization Energy -17.5294 kcal/mol@LB@Unfolding Energy - mRNA 1.22878 kcal/mol@LB@Unfolding Energy - sRNA 1.03329 kcal/mol@LB@@LB@Position - mRNA 145 -- 158@LB@Position - sRNA 1 -- 14@LB@Position Seed - mRNA 152 -- 158@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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