Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
49	0.004667	0.3205	b1277	ribA	-9.86	0.291903	241 -- 249	16 -- 24	GTP cyclohydrolase II	_____________240________250\n_____________\|__________\|\n5'-CCA...AAUAC__________UUAUC...UCG-3'\n______________AGAACCAGCA\n______________\|:\|\|\|\|\|\|\|\|\n______________UUUUGGUCGU\n_______3'-UUUU__________CCAUA...UAG-5'\n_____________\|__________\|\n_____________25_________15\n	178 -- 184	214 -- 220	-13.9783 kcal/mol	2.87719 kcal/mol	5.75438 kcal/mol	240 250@LB@ \| \|@LB@5'-CCA...AAUAC UUAUC...UCG-3'@LB@ AGAACCAGCA@LB@ \|:\|\|\|\|\|\|\|\|@LB@ UUUUGGUCGU@LB@ 3'-UUUU CCAUA...UAG-5'@LB@ \| \|@LB@ 25 15@LB@@LB@@LB@Energy -9.86 kcal/mol@LB@Hybridization Energy -13.9783 kcal/mol@LB@Unfolding Energy - mRNA 2.87719 kcal/mol@LB@Unfolding Energy - sRNA 5.75438 kcal/mol@LB@@LB@Position - mRNA 241 -- 249@LB@Position - sRNA 16 -- 24@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 214 -- 220@LB@
50	0.004849	0.3217	b1079	flgH	-12.97	0.070541	130 -- 141	23 -- 34	flagellar protein of basal-body outer-membrane L ring	_____________129____________________142\n_____________\|______________________\|\n5'-ACU...CGAUC__A_____CA____________ACUCU...GGG-3'\n______________AG_UGCUG__AAAACUGACGCA\n______________\|\|_\|\|\|\|\|__\|\|\|\|\|\|\|\|\|\|\|\|\n______________UC_ACGAC__UUUUGACUGCGU\n3'-UUU...UUUUA________UA____________ACCCC...UAG-5'\n_____________\|______________________\|\n_____________35_____________________22\n	135 -- 141	23 -- 29	-26.3449 kcal/mol	4.56982 kcal/mol	12.5314 kcal/mol	129 142@LB@ \| \|@LB@5'-ACU...CGAUC A CA ACUCU...GGG-3'@LB@ AG UGCUG AAAACUGACGCA@LB@ \|\| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|@LB@ UC ACGAC UUUUGACUGCGU@LB@3'-UUU...UUUUA UA ACCCC...UAG-5'@LB@ \| \|@LB@ 35 22@LB@@LB@@LB@Energy -12.97 kcal/mol@LB@Hybridization Energy -26.3449 kcal/mol@LB@Unfolding Energy - mRNA 4.56982 kcal/mol@LB@Unfolding Energy - sRNA 12.5314 kcal/mol@LB@@LB@Position - mRNA 130 -- 141@LB@Position - sRNA 23 -- 34@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 23 -- 29@LB@
51	0.005002	0.3217	b0115	aceF	-12.52	0.087110	146 -- 171	149 -- 173	pyruvate dehydrogenase dihydrolipoyltransacetylase component E2	_____________145______________________________________172\n_____________\|________________________________________\|\n5'-GAU...GCAAU_____________________C_______AUA________CCGCG...CGC-3'\n______________CGCCAA_AUUCA_____ACAU_GAUGCAG___AAGUUAAC\n______________\|:\|\|\|\|_\|:\|\|\|_____\|\|\|\|_\|\|\|\|\|\|\|___\|\|\|\|:\|\|\|\n______________GUGGUU_UGAGU_____UGUA_CUACGUC___UUCAGUUG\n3'-UUU...AAAAU______A_____CAGUG____________CG_________AAAGU...UAG-5'\n_____________\|________________________________________\|\n_____________174______________________________________148\n	165 -- 171	149 -- 155	-31.6788 kcal/mol	5.24034 kcal/mol	12.6269 kcal/mol	145 172@LB@ \| \|@LB@5'-GAU...GCAAU C AUA CCGCG...CGC-3'@LB@ CGCCAA AUUCA ACAU GAUGCAG AAGUUAAC@LB@ \|:\|\|\|\| \|:\|\|\| \|\|\|\| \|\|\|\|\|\|\| \|\|\|\|:\|\|\|@LB@ GUGGUU UGAGU UGUA CUACGUC UUCAGUUG@LB@3'-UUU...AAAAU A CAGUG CG AAAGU...UAG-5'@LB@ \| \|@LB@ 174 148@LB@@LB@@LB@Energy -12.52 kcal/mol@LB@Hybridization Energy -31.6788 kcal/mol@LB@Unfolding Energy - mRNA 5.24034 kcal/mol@LB@Unfolding Energy - sRNA 12.6269 kcal/mol@LB@@LB@Position - mRNA 146 -- 171@LB@Position - sRNA 149 -- 173@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 149 -- 155@LB@
52	0.005023	0.3217	b0403	malZ	-9.64	0.320537	253 -- 286	155 -- 185	maltodextrin glucosidase	_____________252___________________________287\n_____________\|_____________________________\|\n5'-UUU...UCAAC_____________U____C__A_______AGACC...AGC-3'\n______________UGCUCA_UUACAC_GUGG_UG_CGGGCGA\n______________\|:\|\|\|\|_\|:\|\|\|\|_:\|\|:_\|\|_\|:\|\|\|:\|\n______________AUGAGU_AGUGUG_UACU_AC_GUCCGUU\n3'-UUU...UGGUU______C______________________CAGUU...UAG-5'\n_____________\|_____________________________\|\n_____________186___________________________154\n	280 -- 286	155 -- 161	-27.4446 kcal/mol	6.9929 kcal/mol	7.82707 kcal/mol	252 287@LB@ \| \|@LB@5'-UUU...UCAAC U C A AGACC...AGC-3'@LB@ UGCUCA UUACAC GUGG UG CGGGCGA@LB@ \|:\|\|\|\| \|:\|\|\|\| :\|\|: \|\| \|:\|\|\|:\|@LB@ AUGAGU AGUGUG UACU AC GUCCGUU@LB@3'-UUU...UGGUU C CAGUU...UAG-5'@LB@ \| \|@LB@ 186 154@LB@@LB@@LB@Energy -9.64 kcal/mol@LB@Hybridization Energy -27.4446 kcal/mol@LB@Unfolding Energy - mRNA 6.9929 kcal/mol@LB@Unfolding Energy - sRNA 7.82707 kcal/mol@LB@@LB@Position - mRNA 253 -- 286@LB@Position - sRNA 155 -- 185@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 155 -- 161@LB@
53	0.005067	0.3217	b3666	uhpT	-10.07	0.266829	134 -- 177	167 -- 215	hexose phosphate transporter	_____________133_______________________________________________________178\n_____________\|_________________________________________________________\|\n5'-GCC...UGGAC____UCU____CC______________________UA________AUGCC_______GGUAU...UGG-3'\n______________GCUA___CAGG__UGAUUUGCU_GC___UGAUUUU____CAAUGC_____UCACGCA\n______________:\|:\|___\|\|\|\|__\|:\|\|:\|\|\|\|_\|\|___\|\|\|\|\|\|\|____\|\|\|\|:\|_____\|\|\|\|:\|\|\n______________UGGU___GUCC__AUUAGACGA_CG___ACUAAAA____GUUAUG_____AGUGUGU\n____3'-UUUUUUU____C______AU_________C__CCC_______UGUG______AGUC________ACUAC...UAG-5'\n_____________\|_________________________________________________________\|\n_____________216_______________________________________________________166\n	171 -- 177	167 -- 173	-37.7334 kcal/mol	11.6521 kcal/mol	18.4949 kcal/mol	133 178@LB@ \| \|@LB@5'-GCC...UGGAC UCU CC UA AUGCC GGUAU...UGG-3'@LB@ GCUA CAGG UGAUUUGCU GC UGAUUUU CAAUGC UCACGCA@LB@ :\|:\| \|\|\|\| \|:\|\|:\|\|\|\| \|\| \|\|\|\|\|\|\| \|\|\|\|:\| \|\|\|\|:\|\|@LB@ UGGU GUCC AUUAGACGA CG ACUAAAA GUUAUG AGUGUGU@LB@ 3'-UUUUUUU C AU C CCC UGUG AGUC ACUAC...UAG-5'@LB@ \| \|@LB@ 216 166@LB@@LB@@LB@Energy -10.07 kcal/mol@LB@Hybridization Energy -37.7334 kcal/mol@LB@Unfolding Energy - mRNA 11.6521 kcal/mol@LB@Unfolding Energy - sRNA 18.4949 kcal/mol@LB@@LB@Position - mRNA 134 -- 177@LB@Position - sRNA 167 -- 215@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 167 -- 173@LB@
54	0.005402	0.3247	b3054	ygiF	-14.97	0.027422	5 -- 80	111 -- 177	inorganic triphosphatase	_____________4_______________________________________________________________________________81\n_____________\|_______________________________________________________________________________\|\n_______5'-GGCA_____GU___UAC_____AA___U______AUCAA_______CCUCUG___CA____U_G_______U___AA______CGCCC...CCG-3'\n______________UCAGU__CGC_____GCA__GCA__UCGAC_____UCGGAAU______UGU__GGUC_U_CCACUGC_UCA__CUUUUA\n______________\|\|\|\|\|__\|:\|_____\|\|\|__\|\|\|__\|\|:\|\|_____\|\|:\|\|\|\|______\|\|:__\|\|\|\|_\|_\|\|\|\|\|\|\|_\|\|\|__\|:\|\|:\|\n______________AGUCA__GUG_____CGU__CGU__AGUUG_____AGUCUUA______ACG__CCAG_A_GGUGACG_AGU__GGAAGU\n3'-UUU...UUAUG_____GU___UACUA___C____UC_____AA_________________________U_G_______U___CA______UCGUA...UAG-5'\n_____________\|_______________________________________________________________________________\|\n_____________178_____________________________________________________________________________110\n	62 -- 68	123 -- 129	-50.4846 kcal/mol	15.6316 kcal/mol	23.3828 kcal/mol	4 81@LB@ \| \|@LB@ 5'-GGCA GU UAC AA U AUCAA CCUCUG CA U G U AA CGCCC...CCG-3'@LB@ UCAGU CGC GCA GCA UCGAC UCGGAAU UGU GGUC U CCACUGC UCA CUUUUA@LB@ \|\|\|\|\| \|:\| \|\|\| \|\|\| \|\|:\|\| \|\|:\|\|\|\| \|\|: \|\|\|\| \| \|\|\|\|\|\|\| \|\|\| \|:\|\|:\|@LB@ AGUCA GUG CGU CGU AGUUG AGUCUUA ACG CCAG A GGUGACG AGU GGAAGU@LB@3'-UUU...UUAUG GU UACUA C UC AA U G U CA UCGUA...UAG-5'@LB@ \| \|@LB@ 178 110@LB@@LB@@LB@Energy -14.97 kcal/mol@LB@Hybridization Energy -50.4846 kcal/mol@LB@Unfolding Energy - mRNA 15.6316 kcal/mol@LB@Unfolding Energy - sRNA 23.3828 kcal/mol@LB@@LB@Position - mRNA 5 -- 80@LB@Position - sRNA 111 -- 177@LB@Position Seed - mRNA 62 -- 68@LB@Position Seed - sRNA 123 -- 129@LB@
55	0.005617	0.3247	b2829	ptsP	-11.41	0.146041	68 -- 133	122 -- 174	PEP-protein phosphotransferase enzyme I; GAF domain containing protein	______________68____________________________________________________________134\n______________\|_____________________________________________________________\|\n___________5'-_______UACACCAGA__U_____G_______AUG___AAGU_________C______G___GGUGG...UGG-3'\n______________CCAGCAG_________GU_UGA_C_GCUGGCG___GGU____UACUGGUAU_CGGUCA_ACA\n______________\|\|\|\|\|\|\|_________\|\|_\|:\|_\|_\|\|\|\|\|\|\|___\|:\|____:\|\|:::\|\|:_\|:\|\|\|\|_\|\|\|\n______________GGUCGUC_________CA_AUU_G_CGACCGC___CUA____GUGGUUAUG_GUCAGU_UGU\n___3'-UUUUUUUU__________________U___A_A_______CCA___AAAU_________A______G___ACUAC...UAG-5'\n_____________\|______________________________________________________________\|\n_____________175____________________________________________________________121\n	25 -- 31	197 -- 203	-46.8098 kcal/mol	17.4414 kcal/mol	18.4949 kcal/mol	68 134@LB@ \| \|@LB@ 5'- UACACCAGA U G AUG AAGU C G GGUGG...UGG-3'@LB@ CCAGCAG GU UGA C GCUGGCG GGU UACUGGUAU CGGUCA ACA@LB@ \|\|\|\|\|\|\| \|\| \|:\| \| \|\|\|\|\|\|\| \|:\| :\|\|:::\|\|: \|:\|\|\|\| \|\|\|@LB@ GGUCGUC CA AUU G CGACCGC CUA GUGGUUAUG GUCAGU UGU@LB@ 3'-UUUUUUUU U A A CCA AAAU A G ACUAC...UAG-5'@LB@ \| \|@LB@ 175 121@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -46.8098 kcal/mol@LB@Unfolding Energy - mRNA 17.4414 kcal/mol@LB@Unfolding Energy - sRNA 18.4949 kcal/mol@LB@@LB@Position - mRNA 68 -- 133@LB@Position - sRNA 122 -- 174@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 197 -- 203@LB@
56	0.005619	0.3247	b2781	mazG	-9.54	0.334798	182 -- 193	141 -- 152	nucleoside triphosphate pyrophosphohydrolase	_____________181__________194\n_____________\|____________\|\n5'-UGA...ACCCG____________CAAUU...CGC-3'\n______________CAAUUUUCAGGA\n______________\|\|\|:\|\|\|\|\|\|:\|\n______________GUUGAAAGUCUU\n3'-UUU...GUUCA____________AACGC...UAG-5'\n_____________\|____________\|\n_____________153__________140\n	187 -- 193	141 -- 147	-13.182 kcal/mol	1.77322 kcal/mol	1.81388 kcal/mol	181 194@LB@ \| \|@LB@5'-UGA...ACCCG CAAUU...CGC-3'@LB@ CAAUUUUCAGGA@LB@ \|\|\|:\|\|\|\|\|\|:\|@LB@ GUUGAAAGUCUU@LB@3'-UUU...GUUCA AACGC...UAG-5'@LB@ \| \|@LB@ 153 140@LB@@LB@@LB@Energy -9.54 kcal/mol@LB@Hybridization Energy -13.182 kcal/mol@LB@Unfolding Energy - mRNA 1.77322 kcal/mol@LB@Unfolding Energy - sRNA 1.81388 kcal/mol@LB@@LB@Position - mRNA 182 -- 193@LB@Position - sRNA 141 -- 152@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 141 -- 147@LB@
57	0.00565	0.3247	b2081	yegQ	-17.30	0.009213	136 -- 160	1 -- 21	putative peptidase	_____________135_______________________161\n_____________\|_________________________\|\n5'-AUU...AAGUG_______U_____AACC________AGGUG...GUU-3'\n______________GGGGCAC_CCCCU____GCUUCAUC\n______________\|\|\|\|\|\|\|_\|\|\|\|\|____\|\|\|\|\|\|\|\|\n______________CCCCGUG_GGGGA____CGAAGUAG\n3'-UUU...GCGUA_____________A___________-5'\n_____________\|________________________\|\n_____________22_______________________1\n	154 -- 160	1 -- 7	-36.7075 kcal/mol	11.7869 kcal/mol	10.2895 kcal/mol	135 161@LB@ \| \|@LB@5'-AUU...AAGUG U AACC AGGUG...GUU-3'@LB@ GGGGCAC CCCCU GCUUCAUC@LB@ \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\|@LB@ CCCCGUG GGGGA CGAAGUAG@LB@3'-UUU...GCGUA A -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -17.30 kcal/mol@LB@Hybridization Energy -36.7075 kcal/mol@LB@Unfolding Energy - mRNA 11.7869 kcal/mol@LB@Unfolding Energy - sRNA 10.2895 kcal/mol@LB@@LB@Position - mRNA 136 -- 160@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 1 -- 7@LB@
58	0.005664	0.3247	b1878	flhE	-13.77	0.048330	266 -- 286	140 -- 162	proton seal during flagellar secretion	_____________265_____________________287\n_____________\|_______________________\|\n5'-CGU...AUGUG__________AGUGUA_______UACGC...AUC-3'\n______________GCAGGCAAGU________GGAAU\n______________\|\|\|\|\|\|\|\|\|\|________:\|\|\|\|\n______________CGUCCGUUCA________UCUUA\n3'-UUU...UACUA__________GUUGAAAG_____ACGCC...UAG-5'\n_____________\|_______________________\|\n_____________163_____________________139\n	269 -- 275	153 -- 159	-20.7539 kcal/mol	2.26514 kcal/mol	7.696 kcal/mol	265 287@LB@ \| \|@LB@5'-CGU...AUGUG AGUGUA UACGC...AUC-3'@LB@ GCAGGCAAGU GGAAU@LB@ \|\|\|\|\|\|\|\|\|\| :\|\|\|\|@LB@ CGUCCGUUCA UCUUA@LB@3'-UUU...UACUA GUUGAAAG ACGCC...UAG-5'@LB@ \| \|@LB@ 163 139@LB@@LB@@LB@Energy -13.77 kcal/mol@LB@Hybridization Energy -20.7539 kcal/mol@LB@Unfolding Energy - mRNA 2.26514 kcal/mol@LB@Unfolding Energy - sRNA 7.696 kcal/mol@LB@@LB@Position - mRNA 266 -- 286@LB@Position - sRNA 140 -- 162@LB@Position Seed - mRNA 269 -- 275@LB@Position Seed - sRNA 153 -- 159@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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