Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
180	0.02081	0.3833	b3728	pstS	-9.23	0.378852	18 -- 25	128 -- 135	phosphate ABC transporter periplasmic binding protein	_____________17___________26\n_____________\|____________\|\n5'-AGA...UCCUA____________GUAGC...GUG-3'\n______________UUUUGCUUC_UC\n______________::\|\|\|\|\|\|\|_\|\|\n______________GGAACGAAG_AG\n3'-UUU...GUGGG_________U__-5'\n_____________\|___________\|\n_____________136_________128\n	149 -- 155	4 -- 10	-10.5275 kcal/mol	1.71006 kcal/mol	1.04836 kcal/mol	17 26@LB@ \| \|@LB@5'-AGA...UCCUA GUAGC...GUG-3'@LB@ UUUUGCUUC UC@LB@ ::\|\|\|\|\|\|\| \|\|@LB@ GGAACGAAG AG@LB@3'-UUU...GUGGG U -5'@LB@ \| \|@LB@ 136 128@LB@@LB@@LB@Energy -9.23 kcal/mol@LB@Hybridization Energy -10.5275 kcal/mol@LB@Unfolding Energy - mRNA 1.71006 kcal/mol@LB@Unfolding Energy - sRNA 1.04836 kcal/mol@LB@@LB@Position - mRNA 18 -- 25@LB@Position - sRNA 128 -- 135@LB@Position Seed - mRNA 149 -- 155@LB@Position Seed - sRNA 4 -- 10@LB@
181	0.0209	0.3833	b2233	yfaL	-9.66	0.317681	275 -- 285	50 -- 60	adhesin	_____________274______________286\n_____________\|________________\|\n5'-UAU...UCUCU___AA__G________CGGUC...CAU-3'\n______________GCU__CG_UGUCGCGG\n______________:\|\|__\|\|_\|\|\|\|\|\|\|\|\n______________UGA__GC_ACAGCGCC\n3'-UUU...CGUGU___A___G________AUUUU...UAG-5'\n_____________\|________________\|\n_____________61_______________49\n	279 -- 285	50 -- 56	-20.2435 kcal/mol	4.65708 kcal/mol	6.1451 kcal/mol	274 286@LB@ \| \|@LB@5'-UAU...UCUCU AA G CGGUC...CAU-3'@LB@ GCU CG UGUCGCGG@LB@ :\|\| \|\| \|\|\|\|\|\|\|\|@LB@ UGA GC ACAGCGCC@LB@3'-UUU...CGUGU A G AUUUU...UAG-5'@LB@ \| \|@LB@ 61 49@LB@@LB@@LB@Energy -9.66 kcal/mol@LB@Hybridization Energy -20.2435 kcal/mol@LB@Unfolding Energy - mRNA 4.65708 kcal/mol@LB@Unfolding Energy - sRNA 6.1451 kcal/mol@LB@@LB@Position - mRNA 275 -- 285@LB@Position - sRNA 50 -- 60@LB@Position Seed - mRNA 279 -- 285@LB@Position Seed - sRNA 50 -- 56@LB@
183	0.02111	0.3833	b0461	tomB	-12.41	0.091700	125 -- 158	128 -- 160	Hha toxicity attenuator; conjugation-related protein	_____________124_________________________________________159\n_____________\|___________________________________________\|\n5'-UGC...GGAUG________CCCAAA_G_______AUC____C__C____G____UCUGU...AUC-3'\n______________AAUACUCA______A_ACAUGAU____GCA_AG_UUAA_UUUC\n______________\|\|\|\|\|\|\|\|______\|_\|\|\|\|\|\|\|____\|\|\|_\|\|_\|:\|\|_\|\|\|\|\n______________UUAUGAGU______U_UGUACUA____CGU_UC_AGUU_AAAG\n3'-UUU...UGUGG________CAG____G_______CGUC___________G____UCUUA...UAG-5'\n_____________\|___________________________________________\|\n_____________161_________________________________________127\n	224 -- 230	163 -- 169	-23.2008 kcal/mol	3.84731 kcal/mol	8.26055 kcal/mol	124 159@LB@ \| \|@LB@5'-UGC...GGAUG CCCAAA G AUC C C G UCUGU...AUC-3'@LB@ AAUACUCA A ACAUGAU GCA AG UUAA UUUC@LB@ \|\|\|\|\|\|\|\| \| \|\|\|\|\|\|\| \|\|\| \|\| \|:\|\| \|\|\|\|@LB@ UUAUGAGU U UGUACUA CGU UC AGUU AAAG@LB@3'-UUU...UGUGG CAG G CGUC G UCUUA...UAG-5'@LB@ \| \|@LB@ 161 127@LB@@LB@@LB@Energy -12.41 kcal/mol@LB@Hybridization Energy -23.2008 kcal/mol@LB@Unfolding Energy - mRNA 3.84731 kcal/mol@LB@Unfolding Energy - sRNA 8.26055 kcal/mol@LB@@LB@Position - mRNA 125 -- 158@LB@Position - sRNA 128 -- 160@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 163 -- 169@LB@
184	0.02128	0.3833	b2831	mutH	-12.07	0.107608	142 -- 230	114 -- 189	methyl-directed mismatch repair protein	_____________141______________231\n_____________\|________________\|\n5'-UUC...CUGCG________________AUUGC...CGG-3'\n______________AAUAUUCGG_CACAUA\n______________\|\|\|\|:\|\|:\|_\|\|\|\|:\|\n______________UUAUGAGUC_GUGUGU\n3'-UUU...UGUGG_________A______ACUAC...UAG-5'\n_____________\|________________\|\n_____________190______________113\n	154 -- 160	174 -- 180	-15.1193 kcal/mol	2.199 kcal/mol	0.596263 kcal/mol	141 231@LB@ \| \|@LB@5'-UUC...CUGCG AUUGC...CGG-3'@LB@ AAUAUUCGG CACAUA@LB@ \|\|\|\|:\|\|:\| \|\|\|\|:\|@LB@ UUAUGAGUC GUGUGU@LB@3'-UUU...UGUGG A ACUAC...UAG-5'@LB@ \| \|@LB@ 190 113@LB@@LB@@LB@Energy -12.07 kcal/mol@LB@Hybridization Energy -15.1193 kcal/mol@LB@Unfolding Energy - mRNA 2.199 kcal/mol@LB@Unfolding Energy - sRNA 0.596263 kcal/mol@LB@@LB@Position - mRNA 142 -- 230@LB@Position - sRNA 114 -- 189@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 174 -- 180@LB@
185	0.02128	0.3833	b2276	nuoN	-8.72	0.461673	62 -- 81	196 -- 211	NADH:ubiquinone oxidoreductase membrane subunit N	_____________61______________82\n_____________\|_______________\|\n5'-GUA...UAUGA__________U____GUGCU...AUC-3'\n______________UC_CUGUUGC_GGUG\n______________\|\|_\|\|\|\|:\|\|_\|\|\|:\n______________AG_GACAGCG_CCAU\n3'-UUU...GUUGA__C____________UUUAU...UAG-5'\n_____________\|_______________\|\n_____________212_____________195\n	69 -- 75	52 -- 58	-15.8669 kcal/mol	5.2118 kcal/mol	5.88061 kcal/mol	61 82@LB@ \| \|@LB@5'-GUA...UAUGA U GUGCU...AUC-3'@LB@ UC CUGUUGC GGUG@LB@ \|\| \|\|\|\|:\|\| \|\|\|:@LB@ AG GACAGCG CCAU@LB@3'-UUU...GUUGA C UUUAU...UAG-5'@LB@ \| \|@LB@ 212 195@LB@@LB@@LB@Energy -8.72 kcal/mol@LB@Hybridization Energy -15.8669 kcal/mol@LB@Unfolding Energy - mRNA 5.2118 kcal/mol@LB@Unfolding Energy - sRNA 5.88061 kcal/mol@LB@@LB@Position - mRNA 62 -- 81@LB@Position - sRNA 196 -- 211@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 52 -- 58@LB@
186	0.02152	0.3833	b3581	sgbH	-10.17	0.255482	135 -- 158	123 -- 153	3-keto-L-gulonate 6-phosphate decarboxylase	_____________134________159\n_____________\|__________\|\n5'-UUU...UAAGG__________UAUGA...AAG-3'\n______________AG_CACACAU\n______________\|\|_\|\|\|\|\|\|\|\n______________UC_GUGUGUA\n3'-UUU...AUGAG__A_______CUACG...UAG-5'\n_____________\|__________\|\n_____________154________122\n	193 -- 199	166 -- 172	-9.15702 kcal/mol	1.98532 kcal/mol	0.591348 kcal/mol	134 159@LB@ \| \|@LB@5'-UUU...UAAGG UAUGA...AAG-3'@LB@ AG CACACAU@LB@ \|\| \|\|\|\|\|\|\|@LB@ UC GUGUGUA@LB@3'-UUU...AUGAG A CUACG...UAG-5'@LB@ \| \|@LB@ 154 122@LB@@LB@@LB@Energy -10.17 kcal/mol@LB@Hybridization Energy -9.15702 kcal/mol@LB@Unfolding Energy - mRNA 1.98532 kcal/mol@LB@Unfolding Energy - sRNA 0.591348 kcal/mol@LB@@LB@Position - mRNA 135 -- 158@LB@Position - sRNA 123 -- 153@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 166 -- 172@LB@
187	0.02173	0.3833	b3679	yidK	-10.16	0.256396	240 -- 256	191 -- 205	putative transporter	_____________239________________________________________257\n_____________\|__________________________________________\|\n5'-UUU...UUACG_________GC______________U____GAA_________AAACA...AAG-3'\n______________CUGCUGGUG__GGUGAU____CACC_GGUG___GGUC_CGCA\n______________\|\|\|\|\|\|\|:\|__\|\|\|\|\|\|____\|\|\|\|_::\|:___:\|\|\|_\|:\|\|\n______________GACGACCGC__CCACUA____GUGG_UUAU___UCAG_GUGU\n3'-UUU...UAUUA_________________AAAU_________GAG____U____ACUAC...UAG-5'\n_____________\|__________________________________________\|\n_____________206________________________________________190\n	242 -- 248	197 -- 203	-39.2862 kcal/mol	16.885 kcal/mol	15.3993 kcal/mol	239 257@LB@ \| \|@LB@5'-UUU...UUACG GC U GAA AAACA...AAG-3'@LB@ CUGCUGGUG GGUGAU CACC GGUG GGUC CGCA@LB@ \|\|\|\|\|\|\|:\| \|\|\|\|\|\| \|\|\|\| ::\|: :\|\|\| \|:\|\|@LB@ GACGACCGC CCACUA GUGG UUAU UCAG GUGU@LB@3'-UUU...UAUUA AAAU GAG U ACUAC...UAG-5'@LB@ \| \|@LB@ 206 190@LB@@LB@@LB@Energy -10.16 kcal/mol@LB@Hybridization Energy -39.2862 kcal/mol@LB@Unfolding Energy - mRNA 16.885 kcal/mol@LB@Unfolding Energy - sRNA 15.3993 kcal/mol@LB@@LB@Position - mRNA 240 -- 256@LB@Position - sRNA 191 -- 205@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 197 -- 203@LB@
188	0.0218	0.3833	b3357	crp	-15.41	0.022266	196 -- 257	123 -- 172	cAMP-activated global transcription factor mediator of catabolite repression	_____________195_________________258\n_____________\|___________________\|\n5'-GAC...AUAAC____________UU_____ACCGC...AGG-3'\n______________CGCGCAUGGUGC__GGCAA\n______________\|:\|:\|\|\|\|:\|\|\|__\|\|\|\|\|\n______________GUGUGUACUACG__CCGUU\n3'-UUU...AGUCA____________U______CAGUU...UAG-5'\n_____________\|___________________\|\n_____________173_________________122\n	201 -- 207	161 -- 167	-26.7729 kcal/mol	8.17538 kcal/mol	7.52835 kcal/mol	195 258@LB@ \| \|@LB@5'-GAC...AUAAC UU ACCGC...AGG-3'@LB@ CGCGCAUGGUGC GGCAA@LB@ \|:\|:\|\|\|\|:\|\|\| \|\|\|\|\|@LB@ GUGUGUACUACG CCGUU@LB@3'-UUU...AGUCA U CAGUU...UAG-5'@LB@ \| \|@LB@ 173 122@LB@@LB@@LB@Energy -15.41 kcal/mol@LB@Hybridization Energy -26.7729 kcal/mol@LB@Unfolding Energy - mRNA 8.17538 kcal/mol@LB@Unfolding Energy - sRNA 7.52835 kcal/mol@LB@@LB@Position - mRNA 196 -- 257@LB@Position - sRNA 123 -- 172@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 161 -- 167@LB@
189	0.02188	0.3833	b4033	malF	-11.66	0.130598	273 -- 284	194 -- 205	maltose transporter subunit	_____________272__________285\n_____________\|____________\|\n5'-CUG...UCGGC____________UACCU...UAA-3'\n______________CUGCUGGUGGGU\n______________\|\|\|\|\|\|\|:\|\|\|\|\n______________GACGACCGCCCA\n3'-UUU...UAUUA____________CUAAA...UAG-5'\n_____________\|____________\|\n_____________206__________193\n	278 -- 284	194 -- 200	-24.1506 kcal/mol	7.59361 kcal/mol	9.05892 kcal/mol	272 285@LB@ \| \|@LB@5'-CUG...UCGGC UACCU...UAA-3'@LB@ CUGCUGGUGGGU@LB@ \|\|\|\|\|\|\|:\|\|\|\|@LB@ GACGACCGCCCA@LB@3'-UUU...UAUUA CUAAA...UAG-5'@LB@ \| \|@LB@ 206 193@LB@@LB@@LB@Energy -11.66 kcal/mol@LB@Hybridization Energy -24.1506 kcal/mol@LB@Unfolding Energy - mRNA 7.59361 kcal/mol@LB@Unfolding Energy - sRNA 9.05892 kcal/mol@LB@@LB@Position - mRNA 273 -- 284@LB@Position - sRNA 194 -- 205@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 194 -- 200@LB@
190	0.02217	0.3833	b2754	ygbF	-10.33	0.238061	176 -- 225	124 -- 165	CRISPR adaptation ssRNA endonuclease	_____________175____________226\n_____________\|______________\|\n5'-UGG...AAAAU________GC____ACGAG...GUG-3'\n______________GUACCUCC__GCUU\n______________\|:\|\|\|:\|\|__\|\|\|\|\n______________CGUGGGGG__CGAA\n3'-UUU...UACCC________AA____GUAG-5'\n_____________\|______________\|\n_____________166____________123\n	232 -- 238	11 -- 17	-17.8256 kcal/mol	3.33543 kcal/mol	6.90657 kcal/mol	175 226@LB@ \| \|@LB@5'-UGG...AAAAU GC ACGAG...GUG-3'@LB@ GUACCUCC GCUU@LB@ \|:\|\|\|:\|\| \|\|\|\|@LB@ CGUGGGGG CGAA@LB@3'-UUU...UACCC AA GUAG-5'@LB@ \| \|@LB@ 166 123@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -17.8256 kcal/mol@LB@Unfolding Energy - mRNA 3.33543 kcal/mol@LB@Unfolding Energy - sRNA 6.90657 kcal/mol@LB@@LB@Position - mRNA 176 -- 225@LB@Position - sRNA 124 -- 165@LB@Position Seed - mRNA 232 -- 238@LB@Position Seed - sRNA 11 -- 17@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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