Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
173	0.01972	0.3669	b3426	glpD	-5.91	0.203159	207 -- 224	3 -- 20	sn-glycerol-3-phosphate dehydrogenase aerobic FAD/NAD(P)-binding	_____________206_________________225\n_____________\|___________________\|\n5'-AAC...UGGAA____A_________U____AUAGG...AGG-3'\n______________ACCA__AGAUCUGA_UGUG\n______________\|\|\|\|__\|:\|\|\|\|\|\|_\|\|\|\|\n______________UGGU__UUUAGACU_ACAC\n3'-UUU...CAAUG____CC_____________AA-5'\n_____________\|___________________\|\n_____________21__________________2\n	213 -- 219	7 -- 13	-16.1802 kcal/mol	2.74973 kcal/mol	7.52321 kcal/mol	206 225@LB@ \| \|@LB@5'-AAC...UGGAA A U AUAGG...AGG-3'@LB@ ACCA AGAUCUGA UGUG@LB@ \|\|\|\| \|:\|\|\|\|\|\| \|\|\|\|@LB@ UGGU UUUAGACU ACAC@LB@3'-UUU...CAAUG CC AA-5'@LB@ \| \|@LB@ 21 2@LB@@LB@@LB@Energy -5.91 kcal/mol@LB@Hybridization Energy -16.1802 kcal/mol@LB@Unfolding Energy - mRNA 2.74973 kcal/mol@LB@Unfolding Energy - sRNA 7.52321 kcal/mol@LB@@LB@Position - mRNA 207 -- 224@LB@Position - sRNA 3 -- 20@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 7 -- 13@LB@
99	0.007347	0.239	b4348	hsdS	-4.18	0.460078	133 -- 139	70 -- 76	specificity determinant for hsdM and hsdR	_____________132_____140\n_____________\|_______\|\n5'-CUG...GUGAA_______CGAGC...UAA-3'\n______________CUGGGGG\n______________\|\|\|:\|\|\|\n______________GACUCCC\n3'-UUU...GCUGG_______CCAGC...CAA-5'\n_____________\|_______\|\n_____________77______69\n	133 -- 139	70 -- 76	-10.3039 kcal/mol	2.51492 kcal/mol	3.60488 kcal/mol	132 140@LB@ \| \|@LB@5'-CUG...GUGAA CGAGC...UAA-3'@LB@ CUGGGGG@LB@ \|\|\|:\|\|\|@LB@ GACUCCC@LB@3'-UUU...GCUGG CCAGC...CAA-5'@LB@ \| \|@LB@ 77 69@LB@@LB@@LB@Energy -4.18 kcal/mol@LB@Hybridization Energy -10.3039 kcal/mol@LB@Unfolding Energy - mRNA 2.51492 kcal/mol@LB@Unfolding Energy - sRNA 3.60488 kcal/mol@LB@@LB@Position - mRNA 133 -- 139@LB@Position - sRNA 70 -- 76@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 70 -- 76@LB@
29	0.000834	0.09021	b2798	ygdG	-11.41	0.005967	211 -- 245	24 -- 57	Ssb-binding protein misidentified as ExoIX	_____________210_________________________________246\n_____________\|___________________________________\|\n5'-CCA...UGUUC_________UUGUC__U_____GAAUCUU______CGCAU...UCG-3'\n______________AUUUGCUUA_____GA_GCACU_______AUUCGU\n______________\|:\|\|\|\|\|\|\|_____\|\|_\|\|\|\|\|_______\|\|\|\|\|\|\n______________UGAACGAAU_____CU_CGUGA_______UAAGCA\n3'-UUU...UACUU_________UCGUU__U_____AUUUUU_______AUGUG...CAA-5'\n_____________\|___________________________________\|\n_____________58__________________________________23\n	213 -- 219	49 -- 55	-23.2646 kcal/mol	5.57825 kcal/mol	6.27607 kcal/mol	210 246@LB@ \| \|@LB@5'-CCA...UGUUC UUGUC U GAAUCUU CGCAU...UCG-3'@LB@ AUUUGCUUA GA GCACU AUUCGU@LB@ \|:\|\|\|\|\|\|\| \|\| \|\|\|\|\| \|\|\|\|\|\|@LB@ UGAACGAAU CU CGUGA UAAGCA@LB@3'-UUU...UACUU UCGUU U AUUUUU AUGUG...CAA-5'@LB@ \| \|@LB@ 58 23@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -23.2646 kcal/mol@LB@Unfolding Energy - mRNA 5.57825 kcal/mol@LB@Unfolding Energy - sRNA 6.27607 kcal/mol@LB@@LB@Position - mRNA 211 -- 245@LB@Position - sRNA 24 -- 57@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 49 -- 55@LB@
190	0.02239	0.3796	b0724	sdhB	-6.89	0.117799	143 -- 176	24 -- 60	succinate dehydrogenase FeS subunit	_____________142____________________________________177\n_____________\|______________________________________\|\n5'-UUU...GAACC_________GCCC____UUCC____C____________ACUUA...UGA-3'\n______________GAAAC_UGC____GGCA_____CGC___GAAGAUUCGU\n______________\|\|\|\|\|_\|\|\|____:\|\|\|_____\|:\|___:\|\|:\|\|\|\|\|\|\n______________CUUUG_ACG____UCGU_____GUG___UUUUUAAGCA\n3'-UUU...CCCUA_____A___AAU_____UCUUC___AAU__________AUGUG...CAA-5'\n_____________\|______________________________________\|\n_____________61_____________________________________23\n	170 -- 176	24 -- 30	-22.9017 kcal/mol	9.73835 kcal/mol	6.27609 kcal/mol	142 177@LB@ \| \|@LB@5'-UUU...GAACC GCCC UUCC C ACUUA...UGA-3'@LB@ GAAAC UGC GGCA CGC GAAGAUUCGU@LB@ \|\|\|\|\| \|\|\| :\|\|\| \|:\| :\|\|:\|\|\|\|\|\|@LB@ CUUUG ACG UCGU GUG UUUUUAAGCA@LB@3'-UUU...CCCUA A AAU UCUUC AAU AUGUG...CAA-5'@LB@ \| \|@LB@ 61 23@LB@@LB@@LB@Energy -6.89 kcal/mol@LB@Hybridization Energy -22.9017 kcal/mol@LB@Unfolding Energy - mRNA 9.73835 kcal/mol@LB@Unfolding Energy - sRNA 6.27609 kcal/mol@LB@@LB@Position - mRNA 143 -- 176@LB@Position - sRNA 24 -- 60@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 24 -- 30@LB@
100	0.007523	0.2423	b1744	astE	-9.81	0.018546	117 -- 135	36 -- 53	succinylglutamate desuccinylase	_____________116_________________136\n_____________\|___________________\|\n5'-GGU...GCUGC__GAA______________GGAUG...UGC-3'\n______________GC___GGGCGGGAAGCACU\n______________\|\|___::\|\|::\|\|\|\|\|\|\|\|\n______________CG___UUCGUUCUUCGUGA\n3'-UUU...UUGAA__AA_______________AUUUU...CAA-5'\n_____________\|___________________\|\n_____________54__________________35\n	129 -- 135	36 -- 42	-22.0517 kcal/mol	6.14291 kcal/mol	6.10238 kcal/mol	116 136@LB@ \| \|@LB@5'-GGU...GCUGC GAA GGAUG...UGC-3'@LB@ GC GGGCGGGAAGCACU@LB@ \|\| ::\|\|::\|\|\|\|\|\|\|\|@LB@ CG UUCGUUCUUCGUGA@LB@3'-UUU...UUGAA AA AUUUU...CAA-5'@LB@ \| \|@LB@ 54 35@LB@@LB@@LB@Energy -9.81 kcal/mol@LB@Hybridization Energy -22.0517 kcal/mol@LB@Unfolding Energy - mRNA 6.14291 kcal/mol@LB@Unfolding Energy - sRNA 6.10238 kcal/mol@LB@@LB@Position - mRNA 117 -- 135@LB@Position - sRNA 36 -- 53@LB@Position Seed - mRNA 129 -- 135@LB@Position Seed - sRNA 36 -- 42@LB@
104	0.008606	0.2665	b0956	matP	-4.56	0.392859	215 -- 228	25 -- 38	Ter macrodomain organizer matS-binding protein	_____________214____________229\n_____________\|______________\|\n5'-UUU...CAACA_________UC___AAAGC...AAG-3'\n______________ACUUGAAAA__UUG\n______________\|\|\|\|:\|\|\|\|__\|:\|\n______________UGAAUUUUU__AGC\n3'-UUU...CUUCG_________UA___AAUGU...CAA-5'\n_____________\|______________\|\n_____________39_____________24\n	216 -- 222	31 -- 37	-8.38693 kcal/mol	2.39574 kcal/mol	1.4343 kcal/mol	214 229@LB@ \| \|@LB@5'-UUU...CAACA UC AAAGC...AAG-3'@LB@ ACUUGAAAA UUG@LB@ \|\|\|\|:\|\|\|\| \|:\|@LB@ UGAAUUUUU AGC@LB@3'-UUU...CUUCG UA AAUGU...CAA-5'@LB@ \| \|@LB@ 39 24@LB@@LB@@LB@Energy -4.56 kcal/mol@LB@Hybridization Energy -8.38693 kcal/mol@LB@Unfolding Energy - mRNA 2.39574 kcal/mol@LB@Unfolding Energy - sRNA 1.4343 kcal/mol@LB@@LB@Position - mRNA 215 -- 228@LB@Position - sRNA 25 -- 38@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 31 -- 37@LB@
26	0.0007705	0.09021	b3991	thiG	-9.33	0.025562	246 -- 261	25 -- 41	thiamine biosynthesis ThiGH complex subunit	_____________245_______________262\n_____________\|_________________\|\n5'-GCA...UUACC_____A___________CUUCU...CCG-3'\n______________GGCAC__GGGAAAUUCG\n______________:\|\|\|\|__:::\|\|\|\|\|\|\|\n______________UCGUG__UUUUUUAAGC\n3'-UUU...GUUCU_____AA__________AAUGU...CAA-5'\n_____________\|_________________\|\n_____________42________________24\n	255 -- 261	25 -- 31	-14.087 kcal/mol	3.10801 kcal/mol	1.64839 kcal/mol	245 262@LB@ \| \|@LB@5'-GCA...UUACC A CUUCU...CCG-3'@LB@ GGCAC GGGAAAUUCG@LB@ :\|\|\|\| :::\|\|\|\|\|\|\|@LB@ UCGUG UUUUUUAAGC@LB@3'-UUU...GUUCU AA AAUGU...CAA-5'@LB@ \| \|@LB@ 42 24@LB@@LB@@LB@Energy -9.33 kcal/mol@LB@Hybridization Energy -14.087 kcal/mol@LB@Unfolding Energy - mRNA 3.10801 kcal/mol@LB@Unfolding Energy - sRNA 1.64839 kcal/mol@LB@@LB@Position - mRNA 246 -- 261@LB@Position - sRNA 25 -- 41@LB@Position Seed - mRNA 255 -- 261@LB@Position Seed - sRNA 25 -- 31@LB@
174	0.01992	0.3669	b1098	tmk	-8.25	0.051647	183 -- 196	29 -- 42	thymidylate kinase	_____________182____________197\n_____________\|______________\|\n5'-UAA...UCUGA______________AAAAA...GCG-3'\n______________AAGUGCUUAAGGAA\n______________\|\|\|::\|\|\|\|\|::\|\|\n______________UUCGUGAAUUUUUU\n3'-UUU...CGUUC______________AAGCA...CAA-5'\n_____________\|______________\|\n_____________43_____________28\n	190 -- 196	29 -- 35	-11.8447 kcal/mol	1.97362 kcal/mol	1.62369 kcal/mol	182 197@LB@ \| \|@LB@5'-UAA...UCUGA AAAAA...GCG-3'@LB@ AAGUGCUUAAGGAA@LB@ \|\|\|::\|\|\|\|\|::\|\|@LB@ UUCGUGAAUUUUUU@LB@3'-UUU...CGUUC AAGCA...CAA-5'@LB@ \| \|@LB@ 43 28@LB@@LB@@LB@Energy -8.25 kcal/mol@LB@Hybridization Energy -11.8447 kcal/mol@LB@Unfolding Energy - mRNA 1.97362 kcal/mol@LB@Unfolding Energy - sRNA 1.62369 kcal/mol@LB@@LB@Position - mRNA 183 -- 196@LB@Position - sRNA 29 -- 42@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 29 -- 35@LB@
67	0.003475	0.1645	b0416	nusB	-6.41	0.154688	140 -- 154	27 -- 40	transcription antitermination protein	_____________139_____________155\n_____________\|_______________\|\n5'-AAG...UGACC__________U____AAUGU...AGU-3'\n______________GCGCUUGAAA_GAUU\n______________\|\|:\|\|\|:\|\|\|_:\|\|\|\n______________CGUGAAUUUU_UUAA\n3'-UUU...UUCUU_______________GCAAU...CAA-5'\n_____________\|_______________\|\n_____________41______________26\n	142 -- 148	32 -- 38	-12.213 kcal/mol	4.16264 kcal/mol	1.6425 kcal/mol	139 155@LB@ \| \|@LB@5'-AAG...UGACC U AAUGU...AGU-3'@LB@ GCGCUUGAAA GAUU@LB@ \|\|:\|\|\|:\|\|\| :\|\|\|@LB@ CGUGAAUUUU UUAA@LB@3'-UUU...UUCUU GCAAU...CAA-5'@LB@ \| \|@LB@ 41 26@LB@@LB@@LB@Energy -6.41 kcal/mol@LB@Hybridization Energy -12.213 kcal/mol@LB@Unfolding Energy - mRNA 4.16264 kcal/mol@LB@Unfolding Energy - sRNA 1.6425 kcal/mol@LB@@LB@Position - mRNA 140 -- 154@LB@Position - sRNA 27 -- 40@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 32 -- 38@LB@
195	0.02349	0.3864	b3982	nusG	-7.66	0.074558	21 -- 55	24 -- 55	transcription termination factor	_____________20__________________________________56\n_____________\|___________________________________\|\n5'-GAC...UACCG______UU__CCGU_______GUACC_____C___AAGGU...ACA-3'\n______________UUGCUU__GC____GAAGCGC_____GAAGU_CGU\n______________\|\|\|\|\|\|__\|\|____\|\|\|\|\|:\|_____:\|\|:\|_\|\|\|\n______________AACGAA__CG____CUUCGUG_____UUUUA_GCA\n3'-UUU...CUUUG______UU__UU_________AAUU______A___AUGUG...CAA-5'\n_____________\|___________________________________\|\n_____________56__________________________________23\n	35 -- 41	37 -- 43	-23.8738 kcal/mol	9.94572 kcal/mol	6.27216 kcal/mol	20 56@LB@ \| \|@LB@5'-GAC...UACCG UU CCGU GUACC C AAGGU...ACA-3'@LB@ UUGCUU GC GAAGCGC GAAGU CGU@LB@ \|\|\|\|\|\| \|\| \|\|\|\|\|:\| :\|\|:\| \|\|\|@LB@ AACGAA CG CUUCGUG UUUUA GCA@LB@3'-UUU...CUUUG UU UU AAUU A AUGUG...CAA-5'@LB@ \| \|@LB@ 56 23@LB@@LB@@LB@Energy -7.66 kcal/mol@LB@Hybridization Energy -23.8738 kcal/mol@LB@Unfolding Energy - mRNA 9.94572 kcal/mol@LB@Unfolding Energy - sRNA 6.27216 kcal/mol@LB@@LB@Position - mRNA 21 -- 55@LB@Position - sRNA 24 -- 55@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 37 -- 43@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching