Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
44	0.001875	0.1369	b4485	ytfR	-11.10	0.007490	215 -- 227	10 -- 22	putative sugar ABC transporter ATPase		_____________214___________228\n_____________\|_____________\|\n5'-CCU...GACCA_____________CUCCG...GCC-3'\n______________ACACCAGGAGAUC\n______________\|\|\|\|\|\|\|\|\|:\|\|\|\n______________UGUGGUCCUUUAG\n3'-UUU...AGCAA_____________ACUAC...CAA-5'\n_____________\|_____________\|\n_____________23____________9\n	221 -- 227	10 -- 16	-21.5971 kcal/mol	2.94097 kcal/mol	7.55838 kcal/mol	214 228@LB@ \| \|@LB@5'-CCU...GACCA CUCCG...GCC-3'@LB@ ACACCAGGAGAUC@LB@ \|\|\|\|\|\|\|\|\|:\|\|\|@LB@ UGUGGUCCUUUAG@LB@3'-UUU...AGCAA ACUAC...CAA-5'@LB@ \| \|@LB@ 23 9@LB@@LB@@LB@Energy -11.10 kcal/mol@LB@Hybridization Energy -21.5971 kcal/mol@LB@Unfolding Energy - mRNA 2.94097 kcal/mol@LB@Unfolding Energy - sRNA 7.55838 kcal/mol@LB@@LB@Position - mRNA 215 -- 227@LB@Position - sRNA 10 -- 22@LB@Position Seed - mRNA 221 -- 227@LB@Position Seed - sRNA 10 -- 16@LB@
82	0.005534	0.2146	b1342	ydaN	-6.57	0.141434	180 -- 206	30 -- 54	putative Zn(II) transporter		_____________179_________________________207\n_____________\|___________________________\|\n5'-CGU...AAUAA___G____U_AU_________GU____GCGAU...AUG-3'\n______________UGC_UUGA_G__GGAGGCACU__GGAA\n______________\|\|\|_\|\|:\|_\|__:\|\|:\|\|\|\|\|__::\|\|\n______________ACG_AAUU_C__UCUUCGUGA__UUUU\n3'-UUU...UUUGA__________GU_________AU____UAAGC...CAA-5'\n_____________\|___________________________\|\n_____________55__________________________29\n	194 -- 200	36 -- 42	-19.056 kcal/mol	6.29262 kcal/mol	6.19579 kcal/mol	179 207@LB@ \| \|@LB@5'-CGU...AAUAA G U AU GU GCGAU...AUG-3'@LB@ UGC UUGA G GGAGGCACU GGAA@LB@ \|\|\| \|\|:\| \| :\|\|:\|\|\|\|\| ::\|\|@LB@ ACG AAUU C UCUUCGUGA UUUU@LB@3'-UUU...UUUGA GU AU UAAGC...CAA-5'@LB@ \| \|@LB@ 55 29@LB@@LB@@LB@Energy -6.57 kcal/mol@LB@Hybridization Energy -19.056 kcal/mol@LB@Unfolding Energy - mRNA 6.29262 kcal/mol@LB@Unfolding Energy - sRNA 6.19579 kcal/mol@LB@@LB@Position - mRNA 180 -- 206@LB@Position - sRNA 30 -- 54@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 36 -- 42@LB@
20	0.0005034	0.08107	b3962	sthA	-8.00	0.060263	274 -- 291	37 -- 54	pyridine nucleotide transhydrogenase soluble		_____________273________________292\n_____________\|__________________\|\n5'-UAC...GGGCC__G__________U____GCGUC...CAG-3'\n______________UG_UUAAGCAAGG_GCGC\n______________\|\|_\|\|\|\|\|\|\|\|\|:_\|\|:\|\n______________AC_AAUUCGUUCU_CGUG\n3'-UUU...UUUGA__G__________U____AAUUU...CAA-5'\n_____________\|__________________\|\n_____________55_________________36\n	280 -- 286	42 -- 48	-21.3093 kcal/mol	7.14965 kcal/mol	6.1584 kcal/mol	273 292@LB@ \| \|@LB@5'-UAC...GGGCC G U GCGUC...CAG-3'@LB@ UG UUAAGCAAGG GCGC@LB@ \|\| \|\|\|\|\|\|\|\|\|: \|\|:\|@LB@ AC AAUUCGUUCU CGUG@LB@3'-UUU...UUUGA G U AAUUU...CAA-5'@LB@ \| \|@LB@ 55 36@LB@@LB@@LB@Energy -8.00 kcal/mol@LB@Hybridization Energy -21.3093 kcal/mol@LB@Unfolding Energy - mRNA 7.14965 kcal/mol@LB@Unfolding Energy - sRNA 6.1584 kcal/mol@LB@@LB@Position - mRNA 274 -- 291@LB@Position - sRNA 37 -- 54@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 42 -- 48@LB@
164	0.01824	0.3566	b1854	pykA	-7.33	0.090768	1 -- 31	25 -- 60	pyruvate kinase II		______________1___________________________________32\n______________\|___________________________________\|\n___________5'-_____GG__CA_____________________A___CGUUU...ACG-3'\n______________GAAGC__GU______AAGAAGCGCU_GAAGGA_UCG\n______________\|\|\|:\|__\|:______\|\|\|\|\|\|\|:\|\|_:\|\|::\|_\|\|\|\n______________CUUUG__CG______UUCUUCGUGA_UUUUUU_AGC\n3'-UUU...CCCUA_____AA__AAUUCG__________A______A___AAUGU...CAA-5'\n_____________\|____________________________________\|\n_____________61___________________________________24\n	15 -- 21	36 -- 42	-23.5606 kcal/mol	9.95433 kcal/mol	6.27609 kcal/mol	1 32@LB@ \| \|@LB@ 5'- GG CA A CGUUU...ACG-3'@LB@ GAAGC GU AAGAAGCGCU GAAGGA UCG@LB@ \|\|\|:\| \|: \|\|\|\|\|\|\|:\|\| :\|\|::\| \|\|\|@LB@ CUUUG CG UUCUUCGUGA UUUUUU AGC@LB@3'-UUU...CCCUA AA AAUUCG A A AAUGU...CAA-5'@LB@ \| \|@LB@ 61 24@LB@@LB@@LB@Energy -7.33 kcal/mol@LB@Hybridization Energy -23.5606 kcal/mol@LB@Unfolding Energy - mRNA 9.95433 kcal/mol@LB@Unfolding Energy - sRNA 6.27609 kcal/mol@LB@@LB@Position - mRNA 1 -- 31@LB@Position - sRNA 25 -- 60@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 36 -- 42@LB@
193	0.0233	0.3864	b1563	relE	-9.36	0.025073	16 -- 27	29 -- 40	Qin prophage; toxin of the RelE-RelB toxin-antitoxin system		_____________15___________28\n_____________\|____________\|\n5'-GCG...ACGCC____________AUGGG...UAC-3'\n______________GCGCUUGAAAAA\n______________\|\|:\|\|\|:\|\|\|\|\|\n______________CGUGAAUUUUUU\n3'-UUU...UUCUU____________AAGCA...CAA-5'\n_____________\|____________\|\n_____________41___________28\n	21 -- 27	29 -- 35	-12.3855 kcal/mol	1.40487 kcal/mol	1.62106 kcal/mol	15 28@LB@ \| \|@LB@5'-GCG...ACGCC AUGGG...UAC-3'@LB@ GCGCUUGAAAAA@LB@ \|\|:\|\|\|:\|\|\|\|\|@LB@ CGUGAAUUUUUU@LB@3'-UUU...UUCUU AAGCA...CAA-5'@LB@ \| \|@LB@ 41 28@LB@@LB@@LB@Energy -9.36 kcal/mol@LB@Hybridization Energy -12.3855 kcal/mol@LB@Unfolding Energy - mRNA 1.40487 kcal/mol@LB@Unfolding Energy - sRNA 1.62106 kcal/mol@LB@@LB@Position - mRNA 16 -- 27@LB@Position - sRNA 29 -- 40@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 29 -- 35@LB@
148	0.01496	0.3255	b2202	napC	-5.47	0.254772	189 -- 211	26 -- 46	quinol dehydrogenase electron source for NapAB		_____________188_____________________212\n_____________\|_______________________\|\n5'-ACG...GGAAA_______U____A__________UGACC...GUU-3'\n______________UAAGAGG_UAUU_UGGGAAAUUC\n______________:\|\|\|\|:\|_:\|:\|_\|:::\|\|\|\|\|\|\n______________GUUCUUC_GUGA_AUUUUUUAAG\n3'-UUU...AAUUC_______________________CAAUG...CAA-5'\n_____________\|_______________________\|\n_____________47______________________25\n	205 -- 211	26 -- 32	-14.2639 kcal/mol	2.66276 kcal/mol	6.13041 kcal/mol	188 212@LB@ \| \|@LB@5'-ACG...GGAAA U A UGACC...GUU-3'@LB@ UAAGAGG UAUU UGGGAAAUUC@LB@ :\|\|\|\|:\| :\|:\| \|:::\|\|\|\|\|\|@LB@ GUUCUUC GUGA AUUUUUUAAG@LB@3'-UUU...AAUUC CAAUG...CAA-5'@LB@ \| \|@LB@ 47 25@LB@@LB@@LB@Energy -5.47 kcal/mol@LB@Hybridization Energy -14.2639 kcal/mol@LB@Unfolding Energy - mRNA 2.66276 kcal/mol@LB@Unfolding Energy - sRNA 6.13041 kcal/mol@LB@@LB@Position - mRNA 189 -- 211@LB@Position - sRNA 26 -- 46@LB@Position Seed - mRNA 205 -- 211@LB@Position Seed - sRNA 26 -- 32@LB@
46	0.002046	0.1433	b3106	yhaK	-10.10	0.015122	97 -- 120	7 -- 28	redox-sensitive bicupin		_____________96_______________________121\n_____________\|________________________\|\n5'-UCC...GCCAU______CU__U_____________ACAUA...AAU-3'\n______________UUCGUU__CA_UCAGGAAAUUUGA\n______________\|\|\|\|\|\|__\|\|_:\|\|\|\|\|\|\|\|:\|\|\|\n______________AAGCAA__GU_GGUCCUUUAGACU\n3'-UUU...UUUUU______U_________________ACACAA-5'\n_____________\|________________________\|\n_____________29_______________________6\n	111 -- 117	10 -- 16	-23.1033 kcal/mol	4.40095 kcal/mol	8.59864 kcal/mol	96 121@LB@ \| \|@LB@5'-UCC...GCCAU CU U ACAUA...AAU-3'@LB@ UUCGUU CA UCAGGAAAUUUGA@LB@ \|\|\|\|\|\| \|\| :\|\|\|\|\|\|\|\|:\|\|\|@LB@ AAGCAA GU GGUCCUUUAGACU@LB@3'-UUU...UUUUU U ACACAA-5'@LB@ \| \|@LB@ 29 6@LB@@LB@@LB@Energy -10.10 kcal/mol@LB@Hybridization Energy -23.1033 kcal/mol@LB@Unfolding Energy - mRNA 4.40095 kcal/mol@LB@Unfolding Energy - sRNA 8.59864 kcal/mol@LB@@LB@Position - mRNA 97 -- 120@LB@Position - sRNA 7 -- 28@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 10 -- 16@LB@
85	0.005692	0.2157	b4052	dnaB	-8.38	0.047402	89 -- 103	26 -- 40	replicative DNA helicase		_____________88______________104\n_____________\|_______________\|\n5'-UGC...CCACC_______U_______AAUUC...CGG-3'\n______________GUGCUUG_GAAAUUC\n______________\|::\|\|\|:_:\|\|\|\|\|\|\n______________CGUGAAU_UUUUAAG\n3'-UUU...UUCUU_______U_______CAAUG...CAA-5'\n_____________\|_______________\|\n_____________41______________25\n	97 -- 103	26 -- 32	-13.045 kcal/mol	3.01413 kcal/mol	1.64811 kcal/mol	88 104@LB@ \| \|@LB@5'-UGC...CCACC U AAUUC...CGG-3'@LB@ GUGCUUG GAAAUUC@LB@ \|::\|\|\|: :\|\|\|\|\|\|@LB@ CGUGAAU UUUUAAG@LB@3'-UUU...UUCUU U CAAUG...CAA-5'@LB@ \| \|@LB@ 41 25@LB@@LB@@LB@Energy -8.38 kcal/mol@LB@Hybridization Energy -13.045 kcal/mol@LB@Unfolding Energy - mRNA 3.01413 kcal/mol@LB@Unfolding Energy - sRNA 1.64811 kcal/mol@LB@@LB@Position - mRNA 89 -- 103@LB@Position - sRNA 26 -- 40@LB@Position Seed - mRNA 97 -- 103@LB@Position Seed - sRNA 26 -- 32@LB@
69	0.00357	0.1645	b0571	cusR	-5.90	0.203942	86 -- 99	6 -- 21	response regulator in two-component regulatory system with CusS	b1969	_____________85_______________100\n_____________\|________________\|\n5'-AGC...UUACU_______C________UUUCC...GGC-3'\n______________CGCCAGG___UCUGAU\n______________\|:\|\|\|\|\|___\|\|\|\|\|\|\n______________GUGGUCC___AGACUA\n3'-UUU...GCAAU_______UUU______CACAA-5'\n_____________\|________________\|\n_____________22_______________5\n	86 -- 92	15 -- 21	-18.4182 kcal/mol	4.95815 kcal/mol	7.56001 kcal/mol	85 100@LB@ \| \|@LB@5'-AGC...UUACU C UUUCC...GGC-3'@LB@ CGCCAGG UCUGAU@LB@ \|:\|\|\|\|\| \|\|\|\|\|\|@LB@ GUGGUCC AGACUA@LB@3'-UUU...GCAAU UUU CACAA-5'@LB@ \| \|@LB@ 22 5@LB@@LB@@LB@Energy -5.90 kcal/mol@LB@Hybridization Energy -18.4182 kcal/mol@LB@Unfolding Energy - mRNA 4.95815 kcal/mol@LB@Unfolding Energy - sRNA 7.56001 kcal/mol@LB@@LB@Position - mRNA 86 -- 99@LB@Position - sRNA 6 -- 21@LB@Position Seed - mRNA 86 -- 92@LB@Position Seed - sRNA 15 -- 21@LB@
47	0.002119	0.1452	b0694	kdpE	-6.96	0.113004	177 -- 189	26 -- 38	response regulator in two-component regulatory system with KdpD		_____________176___________190\n_____________\|_____________\|\n5'-ACG...CCUGA_____________CAUGA...CGC-3'\n______________ACUUGAAGAAUUU\n______________\|\|\|\|:\|\|:\|\|\|\|:\n______________UGAAUUUUUUAAG\n3'-UUU...CUUCG_____________CAAUG...CAA-5'\n_____________\|_____________\|\n_____________39____________25\n	179 -- 185	30 -- 36	-10.6038 kcal/mol	2.21135 kcal/mol	1.43364 kcal/mol	176 190@LB@ \| \|@LB@5'-ACG...CCUGA CAUGA...CGC-3'@LB@ ACUUGAAGAAUUU@LB@ \|\|\|\|:\|\|:\|\|\|\|:@LB@ UGAAUUUUUUAAG@LB@3'-UUU...CUUCG CAAUG...CAA-5'@LB@ \| \|@LB@ 39 25@LB@@LB@@LB@Energy -6.96 kcal/mol@LB@Hybridization Energy -10.6038 kcal/mol@LB@Unfolding Energy - mRNA 2.21135 kcal/mol@LB@Unfolding Energy - sRNA 1.43364 kcal/mol@LB@@LB@Position - mRNA 177 -- 189@LB@Position - sRNA 26 -- 38@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 30 -- 36@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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