Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
142	0.01047	0.2504	b3998	nfi	-11.68	0.142276	34 -- 51	5 -- 21	endonuclease V; deoxyinosine 3' endonuclease		_____________33_________________52\n_____________\|__________________\|\n5'-UGG...UGCCC_________A________CGACU...GAG-3'\n______________GCAUUGAAG_AGAAGUAG\n______________:\|\|\|::\|\|\|_\|:\|\|\|:\|\|\n______________UGUAGUUUC_UUUUCGUC\n3'-UUU...ACCCC__________________ACCG-5'\n_____________\|__________________\|\n_____________22_________________4\n	45 -- 51	5 -- 11	-17.0993 kcal/mol	3.91468 kcal/mol	1.5073 kcal/mol	33 52@LB@ \| \|@LB@5'-UGG...UGCCC A CGACU...GAG-3'@LB@ GCAUUGAAG AGAAGUAG@LB@ :\|\|\|::\|\|\| \|:\|\|\|:\|\|@LB@ UGUAGUUUC UUUUCGUC@LB@3'-UUU...ACCCC ACCG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -11.68 kcal/mol@LB@Hybridization Energy -17.0993 kcal/mol@LB@Unfolding Energy - mRNA 3.91468 kcal/mol@LB@Unfolding Energy - sRNA 1.5073 kcal/mol@LB@@LB@Position - mRNA 34 -- 51@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 5 -- 11@LB@
175	0.01275	0.2504	b3057	bacA	-10.43	0.234919	238 -- 261	5 -- 26	undecaprenyl pyrophosphate phosphatase		_____________237______________________262\n_____________\|________________________\|\n5'-UUC...AAUAU____U_UG_________UU_A___AAUUU...UUG-3'\n______________UGGG_G__GUCGAAGGA__G_CAG\n______________\|\|\|\|_\|__:\|\|:\|\|\|:\|__\|_\|\|\|\n______________ACCC_C__UAGUUUCUU__C_GUC\n3'-UUU...GUUUU______UG_________UU_____ACCG-5'\n_____________\|________________________\|\n_____________27_______________________4\n	248 -- 254	11 -- 17	-20.3624 kcal/mol	7.43347 kcal/mol	2.50108 kcal/mol	237 262@LB@ \| \|@LB@5'-UUC...AAUAU U UG UU A AAUUU...UUG-3'@LB@ UGGG G GUCGAAGGA G CAG@LB@ \|\|\|\| \| :\|\|:\|\|\|:\| \| \|\|\|@LB@ ACCC C UAGUUUCUU C GUC@LB@3'-UUU...GUUUU UG UU ACCG-5'@LB@ \| \|@LB@ 27 4@LB@@LB@@LB@Energy -10.43 kcal/mol@LB@Hybridization Energy -20.3624 kcal/mol@LB@Unfolding Energy - mRNA 7.43347 kcal/mol@LB@Unfolding Energy - sRNA 2.50108 kcal/mol@LB@@LB@Position - mRNA 238 -- 261@LB@Position - sRNA 5 -- 26@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 11 -- 17@LB@
177	0.01288	0.2504	b3455	livG	-10.61	0.218983	178 -- 198	5 -- 24	branched-chain amino acid ABC transporter ATPase		_____________177___________________199\n_____________\|_____________________\|\n5'-AUG...AAAAC__C___GCG____________GCAUG...UCG-3'\n______________GG_GCA___AAAGGAGAGCAG\n______________\|\|_:\|\|___\|\|\|\|:\|:\|\|\|\|\|\n______________CC_UGU___UUUCUUUUCGUC\n3'-UUU...UUUAC__C___AG_____________ACCG-5'\n_____________\|_____________________\|\n_____________25____________________4\n	192 -- 198	5 -- 11	-19.5574 kcal/mol	6.51739 kcal/mol	2.42705 kcal/mol	177 199@LB@ \| \|@LB@5'-AUG...AAAAC C GCG GCAUG...UCG-3'@LB@ GG GCA AAAGGAGAGCAG@LB@ \|\| :\|\| \|\|\|\|:\|:\|\|\|\|\|@LB@ CC UGU UUUCUUUUCGUC@LB@3'-UUU...UUUAC C AG ACCG-5'@LB@ \| \|@LB@ 25 4@LB@@LB@@LB@Energy -10.61 kcal/mol@LB@Hybridization Energy -19.5574 kcal/mol@LB@Unfolding Energy - mRNA 6.51739 kcal/mol@LB@Unfolding Energy - sRNA 2.42705 kcal/mol@LB@@LB@Position - mRNA 178 -- 198@LB@Position - sRNA 5 -- 24@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 5 -- 11@LB@
11	0.0003708	0.1087	b4201	priB	-14.90	0.029310	176 -- 193	6 -- 26	primosomal protein N		_____________175___________________194\n_____________\|_____________________\|\n5'-AGG...GAAGC__________U__________AUUUC...AUC-3'\n______________UGGGGAU_UC___GAAGAGUA\n______________\|\|\|\|\|\|:_\|\|___\|\|\|:\|\|:\|\n______________ACCCCUG_AG___CUUUUCGU\n3'-UUU...GUUUU_______U__UUU________CACCG-5'\n_____________\|_____________________\|\n_____________27____________________5\n	187 -- 193	6 -- 12	-19.0189 kcal/mol	3.41067 kcal/mol	0.706421 kcal/mol	175 194@LB@ \| \|@LB@5'-AGG...GAAGC U AUUUC...AUC-3'@LB@ UGGGGAU UC GAAGAGUA@LB@ \|\|\|\|\|\|: \|\| \|\|\|:\|\|:\|@LB@ ACCCCUG AG CUUUUCGU@LB@3'-UUU...GUUUU U UUU CACCG-5'@LB@ \| \|@LB@ 27 5@LB@@LB@@LB@Energy -14.90 kcal/mol@LB@Hybridization Energy -19.0189 kcal/mol@LB@Unfolding Energy - mRNA 3.41067 kcal/mol@LB@Unfolding Energy - sRNA 0.706421 kcal/mol@LB@@LB@Position - mRNA 176 -- 193@LB@Position - sRNA 6 -- 26@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 6 -- 12@LB@
30	0.001442	0.1635	b3458	livK	-12.84	0.084462	180 -- 215	6 -- 33	leucine transporter subunit		_____________179___________________________________216\n_____________\|_____________________________________\|\n5'-UGU...AACAU______________UUUUGACU__G___C____U___AAAAC...CGA-3'\n______________CAC_GAAUGGGGAU________AU_AAA_GGAA_GCG\n______________\|\|\|_:\|\|\|\|\|\|\|\|:________\|\|_\|\|\|_\|:\|\|_\|\|:\n______________GUG_UUUACCCCUG________UA_UUU_CUUU_CGU\n3'-UUU...CCGAG___U____________________G________U___CACCG-5'\n_____________\|_____________________________________\|\n_____________34____________________________________5\n	186 -- 192	20 -- 26	-21.4141 kcal/mol	5.49822 kcal/mol	3.07787 kcal/mol	179 216@LB@ \| \|@LB@5'-UGU...AACAU UUUUGACU G C U AAAAC...CGA-3'@LB@ CAC GAAUGGGGAU AU AAA GGAA GCG@LB@ \|\|\| :\|\|\|\|\|\|\|\|: \|\| \|\|\| \|:\|\| \|\|:@LB@ GUG UUUACCCCUG UA UUU CUUU CGU@LB@3'-UUU...CCGAG U G U CACCG-5'@LB@ \| \|@LB@ 34 5@LB@@LB@@LB@Energy -12.84 kcal/mol@LB@Hybridization Energy -21.4141 kcal/mol@LB@Unfolding Energy - mRNA 5.49822 kcal/mol@LB@Unfolding Energy - sRNA 3.07787 kcal/mol@LB@@LB@Position - mRNA 180 -- 215@LB@Position - sRNA 6 -- 33@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 20 -- 26@LB@
32	0.001499	0.1635	b0661	miaB	-12.88	0.082685	213 -- 250	6 -- 36	tRNA-i(6)A37 methylthiotransferase		_____________212____________________________________251\n_____________\|______________________________________\|\n5'-CGC...AAAAA_______UU_AAACC_____C_______U___C_____CGAUU...AAC-3'\n______________CUCCAUA__A_____UGGGG_UGUCAGA_GAA_GAGUA\n______________\|\|\|\|\|:\|__\|_____\|\|\|\|\|_::\|\|\|:\|_\|\|\|_:\|\|:\|\n______________GAGGUGU__U_____ACCCC_GUAGUUU_CUU_UUCGU\n3'-UUU...UACCC_______UU___________U_________________CACCG-5'\n_____________\|______________________________________\|\n_____________37_____________________________________5\n	234 -- 240	14 -- 20	-26.1374 kcal/mol	6.57825 kcal/mol	6.67617 kcal/mol	212 251@LB@ \| \|@LB@5'-CGC...AAAAA UU AAACC C U C CGAUU...AAC-3'@LB@ CUCCAUA A UGGGG UGUCAGA GAA GAGUA@LB@ \|\|\|\|\|:\| \| \|\|\|\|\| ::\|\|\|:\| \|\|\| :\|\|:\|@LB@ GAGGUGU U ACCCC GUAGUUU CUU UUCGU@LB@3'-UUU...UACCC UU U CACCG-5'@LB@ \| \|@LB@ 37 5@LB@@LB@@LB@Energy -12.88 kcal/mol@LB@Hybridization Energy -26.1374 kcal/mol@LB@Unfolding Energy - mRNA 6.57825 kcal/mol@LB@Unfolding Energy - sRNA 6.67617 kcal/mol@LB@@LB@Position - mRNA 213 -- 250@LB@Position - sRNA 6 -- 36@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 14 -- 20@LB@
72	0.004007	0.1953	b2175	mepS	-13.55	0.059767	177 -- 194	6 -- 23	murein DD-endopeptidase space-maker hydrolase mutational suppressor of prc thermosensitivity outer membrane lipoprotein weak murein LD-carboxypeptidase		_____________176________________195\n_____________\|__________________\|\n5'-CAA...GUUAA____U__________A__AACAA...CAA-3'\n______________GGAC_UCAAGGGAAA_CA\n______________\|\|\|\|_\|\|\|\|:\|:\|\|\|_\|\|\n______________CCUG_AGUUUCUUUU_GU\n3'-UUU...UUACC____U__________C__CACCG-5'\n_____________\|__________________\|\n_____________24_________________5\n	185 -- 191	9 -- 15	-17.1277 kcal/mol	2.97845 kcal/mol	0.599171 kcal/mol	176 195@LB@ \| \|@LB@5'-CAA...GUUAA U A AACAA...CAA-3'@LB@ GGAC UCAAGGGAAA CA@LB@ \|\|\|\| \|\|\|\|:\|:\|\|\| \|\|@LB@ CCUG AGUUUCUUUU GU@LB@3'-UUU...UUACC U C CACCG-5'@LB@ \| \|@LB@ 24 5@LB@@LB@@LB@Energy -13.55 kcal/mol@LB@Hybridization Energy -17.1277 kcal/mol@LB@Unfolding Energy - mRNA 2.97845 kcal/mol@LB@Unfolding Energy - sRNA 0.599171 kcal/mol@LB@@LB@Position - mRNA 177 -- 194@LB@Position - sRNA 6 -- 23@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 9 -- 15@LB@
97	0.005972	0.2161	b0768	ybhD	-15.34	0.022873	51 -- 78	6 -- 32	putative DNA-binding transcriptional regulator	b2409	_____________50___________________________79\n_____________\|____________________________\|\n5'-CAG...UUAUU____U____UU__________C______UCUCU...CAU-3'\n______________GCGA_AUGG__ACAUUAAGGG_AAAGCA\n______________:\|:\|_\|\|\|\|__\|\|\|\|:\|\|:\|:_\|\|\|\|\|\|\n______________UGUU_UACC__UGUAGUUUCU_UUUCGU\n3'-UUU...CGAGG____U____CC_________________CACCG-5'\n_____________\|____________________________\|\n_____________33___________________________5\n	64 -- 70	13 -- 19	-24.2506 kcal/mol	6.37495 kcal/mol	2.53457 kcal/mol	50 79@LB@ \| \|@LB@5'-CAG...UUAUU U UU C UCUCU...CAU-3'@LB@ GCGA AUGG ACAUUAAGGG AAAGCA@LB@ :\|:\| \|\|\|\| \|\|\|\|:\|\|:\|: \|\|\|\|\|\|@LB@ UGUU UACC UGUAGUUUCU UUUCGU@LB@3'-UUU...CGAGG U CC CACCG-5'@LB@ \| \|@LB@ 33 5@LB@@LB@@LB@Energy -15.34 kcal/mol@LB@Hybridization Energy -24.2506 kcal/mol@LB@Unfolding Energy - mRNA 6.37495 kcal/mol@LB@Unfolding Energy - sRNA 2.53457 kcal/mol@LB@@LB@Position - mRNA 51 -- 78@LB@Position - sRNA 6 -- 32@LB@Position Seed - mRNA 64 -- 70@LB@Position Seed - sRNA 13 -- 19@LB@
133	0.009634	0.2504	b2551	glyA	-13.83	0.051752	272 -- 286	6 -- 21	serine hydroxymethyltransferase		_____________271______________287\n_____________\|________________\|\n5'-CAA...AAAGU________________CAUCG...UCG-3'\n______________ACGUC_AGGAAGAGCA\n______________\|\|:\|\|_\|:\|\|\|:\|\|\|\|\n______________UGUAG_UUCUUUUCGU\n3'-UUU...ACCCC_____U__________CACCG-5'\n_____________\|________________\|\n_____________22_______________5\n	280 -- 286	6 -- 12	-16.7043 kcal/mol	2.28484 kcal/mol	0.584589 kcal/mol	271 287@LB@ \| \|@LB@5'-CAA...AAAGU CAUCG...UCG-3'@LB@ ACGUC AGGAAGAGCA@LB@ \|\|:\|\| \|:\|\|\|:\|\|\|\|@LB@ UGUAG UUCUUUUCGU@LB@3'-UUU...ACCCC U CACCG-5'@LB@ \| \|@LB@ 22 5@LB@@LB@@LB@Energy -13.83 kcal/mol@LB@Hybridization Energy -16.7043 kcal/mol@LB@Unfolding Energy - mRNA 2.28484 kcal/mol@LB@Unfolding Energy - sRNA 0.584589 kcal/mol@LB@@LB@Position - mRNA 272 -- 286@LB@Position - sRNA 6 -- 21@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 6 -- 12@LB@
159	0.01174	0.2504	b3886	yihY	-12.46	0.100688	17 -- 39	6 -- 26	BrkB family putative transporter inner membrane protein		_____________16______________________40\n_____________\|_______________________\|\n5'-UAU...AGAUC________CG____CCU______CGAAU...CAA-3'\n______________UGGGGAUG__CAAA___GAAGCA\n______________\|\|\|\|\|\|::__\|\|\|\|___:\|\|\|\|\|\n______________ACCCCUGU__GUUU___UUUCGU\n3'-UUU...GUUUU________A_____CU_______CACCG-5'\n_____________\|_______________________\|\n_____________27______________________5\n	18 -- 24	19 -- 25	-18.1196 kcal/mol	4.95346 kcal/mol	0.706421 kcal/mol	16 40@LB@ \| \|@LB@5'-UAU...AGAUC CG CCU CGAAU...CAA-3'@LB@ UGGGGAUG CAAA GAAGCA@LB@ \|\|\|\|\|\|:: \|\|\|\| :\|\|\|\|\|@LB@ ACCCCUGU GUUU UUUCGU@LB@3'-UUU...GUUUU A CU CACCG-5'@LB@ \| \|@LB@ 27 5@LB@@LB@@LB@Energy -12.46 kcal/mol@LB@Hybridization Energy -18.1196 kcal/mol@LB@Unfolding Energy - mRNA 4.95346 kcal/mol@LB@Unfolding Energy - sRNA 0.706421 kcal/mol@LB@@LB@Position - mRNA 17 -- 39@LB@Position - sRNA 6 -- 26@LB@Position Seed - mRNA 18 -- 24@LB@Position Seed - sRNA 19 -- 25@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching