Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
131	0.02569	0.6177	b0848	ybjM	-8.44	0.085177	187 -- 197	44 -- 55	inner membrane protein		_____________186__________198\n_____________\|____________\|\n5'-GAA...GCAGU____________CGAGU...GGG-3'\n______________GUC_AGGAGGAA\n______________\|\|\|_\|:\|\|\|\|\|\|\n______________CAG_UUCUCCUU\n3'-UUU...CCGGG___U________AAAGU...ACA-5'\n_____________\|____________\|\n_____________56___________43\n	191 -- 197	44 -- 50	-13.6185 kcal/mol	4.23971 kcal/mol	0.941432 kcal/mol	186 198@LB@ \| \|@LB@5'-GAA...GCAGU CGAGU...GGG-3'@LB@ GUC AGGAGGAA@LB@ \|\|\| \|:\|\|\|\|\|\|@LB@ CAG UUCUCCUU@LB@3'-UUU...CCGGG U AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -8.44 kcal/mol@LB@Hybridization Energy -13.6185 kcal/mol@LB@Unfolding Energy - mRNA 4.23971 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 187 -- 197@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 44 -- 50@LB@
132	0.02588	0.6177	b3180	yhbY	-5.98	0.252347	290 -- 297	46 -- 53	RNA binding protein associated with pre-50S ribosomal subunits		_____________289______298\n_____________\|________\|\n5'-CUG...UUGAC________UGC-3'\n______________CGAAGGGG\n______________\|:\|\|\|:\|\|\n______________GUUUCUCC\n3'-UUU...GGGCA________UUAAA...ACA-5'\n_____________\|________\|\n_____________54_______45\n	291 -- 297	46 -- 52	-10.2464 kcal/mol	3.35117 kcal/mol	0.910679 kcal/mol	289 298@LB@ \| \|@LB@5'-CUG...UUGAC UGC-3'@LB@ CGAAGGGG@LB@ \|:\|\|\|:\|\|@LB@ GUUUCUCC@LB@3'-UUU...GGGCA UUAAA...ACA-5'@LB@ \| \|@LB@ 54 45@LB@@LB@@LB@Energy -5.98 kcal/mol@LB@Hybridization Energy -10.2464 kcal/mol@LB@Unfolding Energy - mRNA 3.35117 kcal/mol@LB@Unfolding Energy - sRNA 0.910679 kcal/mol@LB@@LB@Position - mRNA 290 -- 297@LB@Position - sRNA 46 -- 53@LB@Position Seed - mRNA 291 -- 297@LB@Position Seed - sRNA 46 -- 52@LB@
133	0.02604	0.6177	b0876	ybjD	-5.23	0.342712	179 -- 199	45 -- 55	putative OLD family ATP-dependent endonuclease; DUF2813 family protein		_____________178___________________200\n_____________\|_____________________\|\n5'-CGU...GAUAA____CAUAUUUCUG_______UAUGA...GGG-3'\n______________GUCA__________GAGGGGA\n______________\|\|\|\|__________:\|\|:\|\|\|\n______________CAGU__________UUCUCCU\n3'-UUU...CCGGG_____________________UAAAG...ACA-5'\n_____________\|_____________________\|\n_____________56____________________44\n	193 -- 199	45 -- 51	-10.7419 kcal/mol	4.58374 kcal/mol	0.930875 kcal/mol	178 200@LB@ \| \|@LB@5'-CGU...GAUAA CAUAUUUCUG UAUGA...GGG-3'@LB@ GUCA GAGGGGA@LB@ \|\|\|\| :\|\|:\|\|\|@LB@ CAGU UUCUCCU@LB@3'-UUU...CCGGG UAAAG...ACA-5'@LB@ \| \|@LB@ 56 44@LB@@LB@@LB@Energy -5.23 kcal/mol@LB@Hybridization Energy -10.7419 kcal/mol@LB@Unfolding Energy - mRNA 4.58374 kcal/mol@LB@Unfolding Energy - sRNA 0.930875 kcal/mol@LB@@LB@Position - mRNA 179 -- 199@LB@Position - sRNA 45 -- 55@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 45 -- 51@LB@
134	0.02619	0.6177	b1126	potA	-9.47	0.052791	285 -- 299	37 -- 55	spermidine/putrescine ABC transporter ATPase		_____________284_________________300\n_____________\|___________________\|\n5'-CAA...UUGAU_GU________________C-3'\n______________G__AAAGAGG____UCAUU\n______________\|__\|\|\|\|\|\|\|____\|\|\|\|\|\n______________C__UUUCUCC____AGUAA\n3'-UUU...CCGGG_AG_______UUAA_____AAAAA...ACA-5'\n_____________\|___________________\|\n_____________56__________________36\n	288 -- 294	46 -- 52	-11.4861 kcal/mol	0.840334 kcal/mol	1.17789 kcal/mol	284 300@LB@ \| \|@LB@5'-CAA...UUGAU GU C-3'@LB@ G AAAGAGG UCAUU@LB@ \| \|\|\|\|\|\|\| \|\|\|\|\|@LB@ C UUUCUCC AGUAA@LB@3'-UUU...CCGGG AG UUAA AAAAA...ACA-5'@LB@ \| \|@LB@ 56 36@LB@@LB@@LB@Energy -9.47 kcal/mol@LB@Hybridization Energy -11.4861 kcal/mol@LB@Unfolding Energy - mRNA 0.840334 kcal/mol@LB@Unfolding Energy - sRNA 1.17789 kcal/mol@LB@@LB@Position - mRNA 285 -- 299@LB@Position - sRNA 37 -- 55@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 46 -- 52@LB@
135	0.02628	0.6177	b3547	yhjX	-9.70	0.047334	158 -- 184	28 -- 55	pyruvate-inducible inner membrane protein putative transporter		_____________157___________________________185\n_____________\|_____________________________\|\n5'-UAA...UGGUU_____C____AUU____C___________CUGCA...UUA-3'\n______________GUCGG_AGAG___UUUC___UUUUUAUUA\n______________\|\|\|::_\|\|\|\|___\|\|\|\|___\|\|\|\|\|\|\|\|\|\n______________CAGUU_UCUC___AAAG___AAAAAUAAU\n3'-UUU...CCGGG__________CUU____UAA_________AAUAC...ACA-5'\n_____________\|_____________________________\|\n_____________56____________________________27\n	178 -- 184	28 -- 34	-15.2509 kcal/mol	4.36169 kcal/mol	1.18814 kcal/mol	157 185@LB@ \| \|@LB@5'-UAA...UGGUU C AUU C CUGCA...UUA-3'@LB@ GUCGG AGAG UUUC UUUUUAUUA@LB@ \|\|\|:: \|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ CAGUU UCUC AAAG AAAAAUAAU@LB@3'-UUU...CCGGG CUU UAA AAUAC...ACA-5'@LB@ \| \|@LB@ 56 27@LB@@LB@@LB@Energy -9.70 kcal/mol@LB@Hybridization Energy -15.2509 kcal/mol@LB@Unfolding Energy - mRNA 4.36169 kcal/mol@LB@Unfolding Energy - sRNA 1.18814 kcal/mol@LB@@LB@Position - mRNA 158 -- 184@LB@Position - sRNA 28 -- 55@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 28 -- 34@LB@
136	0.02642	0.6177	b4036	lamB	-8.19	0.095416	26 -- 54	55 -- 84	maltose outer membrane porin (maltoporin)		_____________25_____________________________55\n_____________\|______________________________\|\n5'-CGC...GGUAG_____________CAU_______C______CCGCC...GUU-3'\n______________AAGAAGGU_GCCA___UCGCUAU_GGCCUG\n______________\|\|:\|\|::\|_\|\|\|\|___\|\|\|\|\|\|\|_\|\|\|\|:\|\n______________UUUUUUUA_CGGU___AGCGAUA_CCGGGC\n___________3'-________C____UAU_______A______AGUUU...ACA-5'\n______________\|_____________________________\|\n______________84____________________________54\n	41 -- 47	62 -- 68	-32.2235 kcal/mol	10.7352 kcal/mol	13.2983 kcal/mol	25 55@LB@ \| \|@LB@5'-CGC...GGUAG CAU C CCGCC...GUU-3'@LB@ AAGAAGGU GCCA UCGCUAU GGCCUG@LB@ \|\|:\|\|::\| \|\|\|\| \|\|\|\|\|\|\| \|\|\|\|:\|@LB@ UUUUUUUA CGGU AGCGAUA CCGGGC@LB@ 3'- C UAU A AGUUU...ACA-5'@LB@ \| \|@LB@ 84 54@LB@@LB@@LB@Energy -8.19 kcal/mol@LB@Hybridization Energy -32.2235 kcal/mol@LB@Unfolding Energy - mRNA 10.7352 kcal/mol@LB@Unfolding Energy - sRNA 13.2983 kcal/mol@LB@@LB@Position - mRNA 26 -- 54@LB@Position - sRNA 55 -- 84@LB@Position Seed - mRNA 41 -- 47@LB@Position Seed - sRNA 62 -- 68@LB@
137	0.02646	0.6177	b3807	cyaY	-4.99	0.375460	213 -- 221	38 -- 46	iron-dependent inhibitor of iron-sulfur cluster formation; frataxin; iron-binding and oxidizing protein		_____________212_______222\n_____________\|_________\|\n5'-UGA...ACAGU_________CGCCU...AAA-3'\n______________GAAUUUCAU\n______________\|\|\|\|\|\|\|\|\|\n______________CUUAAAGUA\n3'-UUU...UUCUC_________AAAAA...ACA-5'\n_____________\|_________\|\n_____________47________37\n	215 -- 221	38 -- 44	-7.89908 kcal/mol	1.90809 kcal/mol	1.00074 kcal/mol	212 222@LB@ \| \|@LB@5'-UGA...ACAGU CGCCU...AAA-3'@LB@ GAAUUUCAU@LB@ \|\|\|\|\|\|\|\|\|@LB@ CUUAAAGUA@LB@3'-UUU...UUCUC AAAAA...ACA-5'@LB@ \| \|@LB@ 47 37@LB@@LB@@LB@Energy -4.99 kcal/mol@LB@Hybridization Energy -7.89908 kcal/mol@LB@Unfolding Energy - mRNA 1.90809 kcal/mol@LB@Unfolding Energy - sRNA 1.00074 kcal/mol@LB@@LB@Position - mRNA 213 -- 221@LB@Position - sRNA 38 -- 46@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 38 -- 44@LB@
138	0.02658	0.6177	b2331	smrB	-9.17	0.060779	224 -- 232	45 -- 53	putative DNA endonuclease		_____________223_______233\n_____________\|_________\|\n5'-UCA...CUUAG_________CCAGG...GAC-3'\n______________CGAGGAGGA\n______________\|:\|:\|\|\|\|\|\n______________GUUUCUCCU\n3'-UUU...GGGCA_________UAAAG...ACA-5'\n_____________\|_________\|\n_____________54________44\n	226 -- 232	45 -- 51	-12.8038 kcal/mol	2.72026 kcal/mol	0.917553 kcal/mol	223 233@LB@ \| \|@LB@5'-UCA...CUUAG CCAGG...GAC-3'@LB@ CGAGGAGGA@LB@ \|:\|:\|\|\|\|\|@LB@ GUUUCUCCU@LB@3'-UUU...GGGCA UAAAG...ACA-5'@LB@ \| \|@LB@ 54 44@LB@@LB@@LB@Energy -9.17 kcal/mol@LB@Hybridization Energy -12.8038 kcal/mol@LB@Unfolding Energy - mRNA 2.72026 kcal/mol@LB@Unfolding Energy - sRNA 0.917553 kcal/mol@LB@@LB@Position - mRNA 224 -- 232@LB@Position - sRNA 45 -- 53@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 45 -- 51@LB@
139	0.02683	0.6177	b1474	fdnG	-8.74	0.074199	188 -- 196	44 -- 52	formate dehydrogenase-N alpha subunit nitrate-inducible	b3894	_____________187_______197\n_____________\|_________\|\n5'-AUU...UGAAA_________AGCAA...CUC-3'\n______________AAAGAGGAA\n______________\|\|\|\|\|\|\|\|\|\n______________UUUCUCCUU\n3'-UUU...GGCAG_________AAAGU...ACA-5'\n_____________\|_________\|\n_____________53________43\n	190 -- 196	44 -- 50	-11.3772 kcal/mol	1.78024 kcal/mol	0.860683 kcal/mol	187 197@LB@ \| \|@LB@5'-AUU...UGAAA AGCAA...CUC-3'@LB@ AAAGAGGAA@LB@ \|\|\|\|\|\|\|\|\|@LB@ UUUCUCCUU@LB@3'-UUU...GGCAG AAAGU...ACA-5'@LB@ \| \|@LB@ 53 43@LB@@LB@@LB@Energy -8.74 kcal/mol@LB@Hybridization Energy -11.3772 kcal/mol@LB@Unfolding Energy - mRNA 1.78024 kcal/mol@LB@Unfolding Energy - sRNA 0.860683 kcal/mol@LB@@LB@Position - mRNA 188 -- 196@LB@Position - sRNA 44 -- 52@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 44 -- 50@LB@
140	0.02703	0.6177	b3447	ggt	-5.41	0.319095	66 -- 81	38 -- 54	gamma-glutamyltranspeptidase		_____________65________________82\n_____________\|_________________\|\n5'-GUU...AAGAA________UC_______CGCGU...CGU-3'\n______________UCAGA_AG__GUUUCAU\n______________\|\|\|:\|_\|\|__:\|\|\|\|\|\|\n______________AGUUU_UC__UAAAGUA\n3'-UUU...CGGGC_____C__CU_______AAAAA...ACA-5'\n_____________\|_________________\|\n_____________55________________37\n	75 -- 81	38 -- 44	-8.61894 kcal/mol	2.03989 kcal/mol	1.17036 kcal/mol	65 82@LB@ \| \|@LB@5'-GUU...AAGAA UC CGCGU...CGU-3'@LB@ UCAGA AG GUUUCAU@LB@ \|\|\|:\| \|\| :\|\|\|\|\|\|@LB@ AGUUU UC UAAAGUA@LB@3'-UUU...CGGGC C CU AAAAA...ACA-5'@LB@ \| \|@LB@ 55 37@LB@@LB@@LB@Energy -5.41 kcal/mol@LB@Hybridization Energy -8.61894 kcal/mol@LB@Unfolding Energy - mRNA 2.03989 kcal/mol@LB@Unfolding Energy - sRNA 1.17036 kcal/mol@LB@@LB@Position - mRNA 66 -- 81@LB@Position - sRNA 38 -- 54@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 38 -- 44@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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