Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
161	0.03295	0.6177	b1630	rsxD	-5.86	0.265655	34 -- 42	49 -- 57	SoxR iron-sulfur cluster reduction factor component; putative membrane protein of electron transport complex		_____________33________43\n_____________\|_________\|\n5'-AGC...AGCUG_________CGGCU...CAG-3'\n______________CCGUCGAAG\n______________\|\|\|\|\|:\|\|\|\n______________GGCAGUUUC\n3'-UUU...AACCG_________UCCUU...ACA-5'\n_____________\|_________\|\n_____________58________48\n	36 -- 42	49 -- 55	-12.0908 kcal/mol	3.50131 kcal/mol	2.72876 kcal/mol	33 43@LB@ \| \|@LB@5'-AGC...AGCUG CGGCU...CAG-3'@LB@ CCGUCGAAG@LB@ \|\|\|\|\|:\|\|\|@LB@ GGCAGUUUC@LB@3'-UUU...AACCG UCCUU...ACA-5'@LB@ \| \|@LB@ 58 48@LB@@LB@@LB@Energy -5.86 kcal/mol@LB@Hybridization Energy -12.0908 kcal/mol@LB@Unfolding Energy - mRNA 3.50131 kcal/mol@LB@Unfolding Energy - sRNA 2.72876 kcal/mol@LB@@LB@Position - mRNA 34 -- 42@LB@Position - sRNA 49 -- 57@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 49 -- 55@LB@
162	0.03332	0.6177	b3304	rplR	-8.58	0.079933	170 -- 177	46 -- 53	50S ribosomal subunit protein L18		_____________169______178\n_____________\|________\|\n5'-AUC...CGUAC________CUAAG...GUC-3'\n______________CAAAGAGG\n______________\|\|\|\|\|\|\|\|\n______________GUUUCUCC\n3'-UUU...GGGCA________UUAAA...ACA-5'\n_____________\|________\|\n_____________54_______45\n	171 -- 177	46 -- 52	-11.2869 kcal/mol	1.80103 kcal/mol	0.910679 kcal/mol	169 178@LB@ \| \|@LB@5'-AUC...CGUAC CUAAG...GUC-3'@LB@ CAAAGAGG@LB@ \|\|\|\|\|\|\|\|@LB@ GUUUCUCC@LB@3'-UUU...GGGCA UUAAA...ACA-5'@LB@ \| \|@LB@ 54 45@LB@@LB@@LB@Energy -8.58 kcal/mol@LB@Hybridization Energy -11.2869 kcal/mol@LB@Unfolding Energy - mRNA 1.80103 kcal/mol@LB@Unfolding Energy - sRNA 0.910679 kcal/mol@LB@@LB@Position - mRNA 170 -- 177@LB@Position - sRNA 46 -- 53@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 46 -- 52@LB@
163	0.03345	0.6177	b1235	rssB	-5.20	0.346272	186 -- 195	44 -- 55	PcnB-degradosome interaction factor; response regulator		_____________185__________196\n_____________\|____________\|\n5'-AAU...AGCCA____________AGAAC...CAA-3'\n______________GUC__AGGGGAG\n______________\|\|\|__\|\|:\|\|\|:\n______________CAG__UCUCCUU\n3'-UUU...CCGGG___UU_______AAAGU...ACA-5'\n_____________\|____________\|\n_____________56___________43\n	189 -- 195	44 -- 50	-10.1709 kcal/mol	4.02871 kcal/mol	0.941432 kcal/mol	185 196@LB@ \| \|@LB@5'-AAU...AGCCA AGAAC...CAA-3'@LB@ GUC AGGGGAG@LB@ \|\|\| \|\|:\|\|\|:@LB@ CAG UCUCCUU@LB@3'-UUU...CCGGG UU AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -5.20 kcal/mol@LB@Hybridization Energy -10.1709 kcal/mol@LB@Unfolding Energy - mRNA 4.02871 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 186 -- 195@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 44 -- 50@LB@
164	0.03346	0.6177	b1449	curA	-5.87	0.264423	114 -- 122	45 -- 53	curcumin/dihydrocurcumin reductase NADPH-dependent		_____________113_______123\n_____________\|_________\|\n5'-CGG...UGUUG_________UGUGA...UCG-3'\n______________CAGAGGGGA\n______________\|\|:\|\|:\|\|\|\n______________GUUUCUCCU\n3'-UUU...GGGCA_________UAAAG...ACA-5'\n_____________\|_________\|\n_____________54________44\n	116 -- 122	45 -- 51	-11.6619 kcal/mol	4.87234 kcal/mol	0.917553 kcal/mol	113 123@LB@ \| \|@LB@5'-CGG...UGUUG UGUGA...UCG-3'@LB@ CAGAGGGGA@LB@ \|\|:\|\|:\|\|\|@LB@ GUUUCUCCU@LB@3'-UUU...GGGCA UAAAG...ACA-5'@LB@ \| \|@LB@ 54 44@LB@@LB@@LB@Energy -5.87 kcal/mol@LB@Hybridization Energy -11.6619 kcal/mol@LB@Unfolding Energy - mRNA 4.87234 kcal/mol@LB@Unfolding Energy - sRNA 0.917553 kcal/mol@LB@@LB@Position - mRNA 114 -- 122@LB@Position - sRNA 45 -- 53@LB@Position Seed - mRNA 116 -- 122@LB@Position Seed - sRNA 45 -- 51@LB@
165	0.0337	0.6177	b3983	rplK	-8.80	0.071896	189 -- 197	41 -- 49	50S ribosomal subunit protein L11		_____________188_______198\n_____________\|_________\|\n5'-ACC...AACUU_________AUAAU...UAA-3'\n______________GAGGAAUUU\n______________\|\|\|\|\|\|\|\|\|\n______________CUCCUUAAA\n3'-UUU...AGUUU_________GUAAA...ACA-5'\n_____________\|_________\|\n_____________50________40\n	191 -- 197	41 -- 47	-11.4231 kcal/mol	1.76644 kcal/mol	0.852225 kcal/mol	188 198@LB@ \| \|@LB@5'-ACC...AACUU AUAAU...UAA-3'@LB@ GAGGAAUUU@LB@ \|\|\|\|\|\|\|\|\|@LB@ CUCCUUAAA@LB@3'-UUU...AGUUU GUAAA...ACA-5'@LB@ \| \|@LB@ 50 40@LB@@LB@@LB@Energy -8.80 kcal/mol@LB@Hybridization Energy -11.4231 kcal/mol@LB@Unfolding Energy - mRNA 1.76644 kcal/mol@LB@Unfolding Energy - sRNA 0.852225 kcal/mol@LB@@LB@Position - mRNA 189 -- 197@LB@Position - sRNA 41 -- 49@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 41 -- 47@LB@
166	0.03384	0.6177	b4035	malK	-4.35	0.473183	186 -- 196	44 -- 53	maltose ABC transportor ATPase		_____________185_________197\n_____________\|___________\|\n5'-CGC...UGUUA_______A___GGGCA...UGU-3'\n______________CAAAGGG_GAA\n______________\|\|\|\|\|:\|_\|\|\|\n______________GUUUCUC_CUU\n3'-UUU...GGGCA___________AAAGU...ACA-5'\n_____________\|___________\|\n_____________54__________43\n	186 -- 192	47 -- 53	-9.12531 kcal/mol	3.84302 kcal/mol	0.927997 kcal/mol	185 197@LB@ \| \|@LB@5'-CGC...UGUUA A GGGCA...UGU-3'@LB@ CAAAGGG GAA@LB@ \|\|\|\|\|:\| \|\|\|@LB@ GUUUCUC CUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -4.35 kcal/mol@LB@Hybridization Energy -9.12531 kcal/mol@LB@Unfolding Energy - mRNA 3.84302 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 186 -- 196@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 47 -- 53@LB@
167	0.03387	0.6177	b3980	tufB	-7.70	0.119070	183 -- 194	44 -- 55	translation elongation factor EF-Tu 2	b3339	_____________182__________195\n_____________\|____________\|\n5'-UCC...UCCGU___UU_______CAAUC...CUA-3'\n______________GUC__AGAGGGA\n______________\|\|\|__\|\|\|\|\|:\|\n______________CAG__UCUCCUU\n3'-UUU...CCGGG___UU_______AAAGU...ACA-5'\n_____________\|____________\|\n_____________56___________43\n	188 -- 194	44 -- 50	-14.3886 kcal/mol	5.74361 kcal/mol	0.941432 kcal/mol	182 195@LB@ \| \|@LB@5'-UCC...UCCGU UU CAAUC...CUA-3'@LB@ GUC AGAGGGA@LB@ \|\|\| \|\|\|\|\|:\|@LB@ CAG UCUCCUU@LB@3'-UUU...CCGGG UU AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -7.70 kcal/mol@LB@Hybridization Energy -14.3886 kcal/mol@LB@Unfolding Energy - mRNA 5.74361 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 183 -- 194@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 44 -- 50@LB@
168	0.03398	0.6177	b0321	yahG	-8.56	0.080369	183 -- 192	44 -- 53	DUF1116 family protein	b4572	_____________182________193\n_____________\|__________\|\n5'-AUC...AUCUG__________GAAGC...AAC-3'\n______________CAAAGGGGAG\n______________\|\|\|\|\|:\|\|\|:\n______________GUUUCUCCUU\n3'-UUU...GGGCA__________AAAGU...ACA-5'\n_____________\|__________\|\n_____________54_________43\n	186 -- 192	44 -- 50	-12.9704 kcal/mol	3.47901 kcal/mol	0.927997 kcal/mol	182 193@LB@ \| \|@LB@5'-AUC...AUCUG GAAGC...AAC-3'@LB@ CAAAGGGGAG@LB@ \|\|\|\|\|:\|\|\|:@LB@ GUUUCUCCUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -8.56 kcal/mol@LB@Hybridization Energy -12.9704 kcal/mol@LB@Unfolding Energy - mRNA 3.47901 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 183 -- 192@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 44 -- 50@LB@
169	0.03425	0.6177	b0336	codB	-12.15	0.014930	188 -- 202	38 -- 52	cytosine transporter		_____________187_____________203\n_____________\|_______________\|\n5'-CCA...GCAAA_______________GUCGC...UUU-3'\n______________AAGGGGGAAUUUCGU\n______________\|\|:\|:\|\|\|\|\|\|\|\|:\|\n______________UUUCUCCUUAAAGUA\n3'-UUU...GGCAG_______________AAAAA...ACA-5'\n_____________\|_______________\|\n_____________53______________37\n	196 -- 202	38 -- 44	-18.3448 kcal/mol	5.13025 kcal/mol	1.0693 kcal/mol	187 203@LB@ \| \|@LB@5'-CCA...GCAAA GUCGC...UUU-3'@LB@ AAGGGGGAAUUUCGU@LB@ \|\|:\|:\|\|\|\|\|\|\|\|:\|@LB@ UUUCUCCUUAAAGUA@LB@3'-UUU...GGCAG AAAAA...ACA-5'@LB@ \| \|@LB@ 53 37@LB@@LB@@LB@Energy -12.15 kcal/mol@LB@Hybridization Energy -18.3448 kcal/mol@LB@Unfolding Energy - mRNA 5.13025 kcal/mol@LB@Unfolding Energy - sRNA 1.0693 kcal/mol@LB@@LB@Position - mRNA 188 -- 202@LB@Position - sRNA 38 -- 52@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 38 -- 44@LB@
170	0.03452	0.6177	b0792	ybhR	-7.08	0.157577	208 -- 236	47 -- 69	putative ABC transporter permease		_____________207____________________________237\n_____________\|______________________________\|\n5'-CAG...GUUUC______UA___ACGUUAA____________UUGCA...AGG-3'\n______________AUCGCU__UGG_______UCCG_CAAAGAG\n______________\|\|\|\|\|\|__\|\|\|_______:\|\|\|_\|\|\|\|\|\|\|\n______________UAGCGA__ACC_______GGGC_GUUUCUC\n3'-UUU...GGUUA______UA______________A_______CUUAA...ACA-5'\n_____________\|______________________________\|\n_____________70_____________________________46\n	230 -- 236	47 -- 53	-23.2437 kcal/mol	4.95079 kcal/mol	11.2154 kcal/mol	207 237@LB@ \| \|@LB@5'-CAG...GUUUC UA ACGUUAA UUGCA...AGG-3'@LB@ AUCGCU UGG UCCG CAAAGAG@LB@ \|\|\|\|\|\| \|\|\| :\|\|\| \|\|\|\|\|\|\|@LB@ UAGCGA ACC GGGC GUUUCUC@LB@3'-UUU...GGUUA UA A CUUAA...ACA-5'@LB@ \| \|@LB@ 70 46@LB@@LB@@LB@Energy -7.08 kcal/mol@LB@Hybridization Energy -23.2437 kcal/mol@LB@Unfolding Energy - mRNA 4.95079 kcal/mol@LB@Unfolding Energy - sRNA 11.2154 kcal/mol@LB@@LB@Position - mRNA 208 -- 236@LB@Position - sRNA 47 -- 69@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 47 -- 53@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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